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NCBI: 26-AUG-2013

Summary[edit source | edit]

  • organism: Staphylococcus aureus N315
  • locus tag: SA2333 [new locus tag: SA_RS13370 ]
  • pan locus tag?: SAUPAN006206000
  • symbol: mvaA
  • pan gene symbol?: mvaA
  • synonym:
  • product: hydroxymethylglutaryl-CoA reductase

Genome View[edit source | edit]

Gene[edit source | edit]

General[edit source | edit]

  • type: CDS
  • locus tag: SA2333 [new locus tag: SA_RS13370 ]
  • symbol: mvaA
  • product: hydroxymethylglutaryl-CoA reductase
  • replicon: chromosome
  • strand: -
  • coordinates: 2617222..2618499
  • length: 1278
  • essential: no DEG other strains

Accession numbers[edit source | edit]

Phenotype[edit source | edit]

  • Share your knowledge and add information here. [edit]

DNA sequence[edit source | edit]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    ATGCAAAGTTTAGATAAGAATTTCCGACATTTATCTCGTCAACAAAAGTTACAACAATTG
    GTAGATAAGCAATGGTTATCAGAAGATCAATTCGACATTTTATTGAATCATCCATTAATT
    GATGAGGAAGTAGCAAATAGTTTAATTGAAAATGTCATCGCGCAAGGTGCATTACCCGTT
    GGATTATTACCGAATATCATTGTGGACGATAAGGCATATGTTGTACCTATGATGGTGGAA
    GAGCCTTCAGTTGTCGCTGCAGCTAGTTATGGTGCAAAGCTAGTGAATCAGACTGGCGGA
    TTTAAAACGGTATCTTCTGAACGTATTATGATAGGTCAAATCGTCTTTGATGGCGTTGAC
    GATACTGAAAAATTATCAGCAGACATTAAAGCTTTAGAAAAGCAAATTCATAAAATTGCG
    GATGAGGCATATCCTTCTATTAAAGCGCGTGGTGGTGGTTACCAACGTATAGCTATTGAT
    ACATTTCCTGAGCAACAGTTACTATCTTTAAAAGTATTTGTTGATACGAAAGATGCTATG
    GGCGCTAATATGCTTAATACGATTTTAGAGGCCATAACTGCATTTTTAAAAAATGAATCT
    CCACAAAGCGACATTTTAATGAGTATTTTATCCAATCATGCAACAGCGTCCGTTGTTAAA
    GTTCAAGGCGAAATTGACGTTAAAGATTTAGCAAGGGGCGAGAGAACTGGAGAAGAGGTT
    GCCAAACGAATGGAACGTGCTTCTGTATTGGCACAAGTTGATATTCATCGTGCTGCAACA
    CATAATAAAGGTGTTATGAATGGCATACATGCCGTTGTTTTAGCAACAGGAAATGATACG
    CGTGGTGCAGAAGCAAGTGCGCATGCATACGCGAGTCGTGACGGACAGTATCGTGGTATT
    GCAACATGGAGATACGATCAAAAACGTCAACGTTTAATTGGTACAATAGAAGTGCCTATG
    ACATTGGCAATCGTTGGCGGTGGTACAAAAGTATTACCAATTGCTAAAGCTTCTTTAGAA
    TTGCTAAATGTAGATTCAGCACAAGAATTAGGTCATGTAGTTGCTGCCGTTGGTTTAGCA
    CAGAACTTTGCAGCATGTCGCGCGCTCGTTTCCGAAGGTATCCAGCAAGGCCATATGAGC
    TTGCAATATAAATCTTTAGCTATTGTTGTAGGTGCAAAAGGTGATGAAATTGCGCAAGTA
    GCTGAAGCATTGAAGCAAGAACCCCGTGCGAATACACAAGTAGCTGAACGCATTTTACAA
    GAAATTAGACAACAATAG
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1200
    1260
    1278

Protein[edit source | edit]

General[edit source | edit]

  • locus tag: SA2333 [new locus tag: SA_RS13370 ]
  • symbol: MvaA
  • description: hydroxymethylglutaryl-CoA reductase
  • length: 425
  • theoretical pI: 6.02407
  • theoretical MW: 46180.5
  • GRAVY: -0.107294

Function[edit source | edit]

  • reaction:
  • TIGRFAM:
    MetabolismEnergy metabolismOtherhydroxymethylglutaryl-CoA reductase, degradative (TIGR00532; EC 1.1.1.88; HMM-score: 330.2)
    MetabolismCentral intermediary metabolismOtherhydroxymethylglutaryl-CoA reductase (NADPH) (TIGR00533; EC 1.1.1.34; HMM-score: 61.3)
    MetabolismTransport and binding proteinsCarbohydrates, organic alcohols, and acids3-hydroxy-3-methylglutaryl-coenzyme A reductase (TIGR00920; HMM-score: 49.7)
  • TheSEED:  
    Fatty Acids, Lipids, and IsoprenoidsIsoprenoidsArchaeal lipids Hydroxymethylglutaryl-CoA reductase (EC 1.1.1.34) 
    IsoprenoidsIsoprenoid Biosynthesis Hydroxymethylglutaryl-CoA reductase (EC 1.1.1.34) 
  • PFAM:
    no clan definedHMG-CoA_red; Hydroxymethylglutaryl-coenzyme A reductase (PF00368; HMM-score: 338)
    UPF0180; Uncharacterised protein family (UPF0180) (PF03698; HMM-score: 16)
    Co_AT_N; Cob(I)alamin adenosyltransferase N terminal (PF12557; HMM-score: 13.5)

Structure, modifications & interactions[edit source | edit]

  • domains:
  • modifications:
  • cofactors:
  • effectors:
  • protein partners:

Localization[edit source | edit]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 7.5
    • Cytoplasmic Membrane Score: 1.15
    • Cellwall Score: 0.62
    • Extracellular Score: 0.73
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular Possibility: 1
    • Signal Peptide Possibility: -1
    • N-terminally Anchored Score: -1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • Ymax: 0.122
    • Ymax_pos: 11
    • Cmax: 0.109
    • Cmax_pos: 57
    • Smax: 0.249
    • Smax_pos: 2
    • Smean: 0.142
    • D: 0.13
  • predicted transmembrane helices (TMHMM): 0

Accession numbers[edit source | edit]

  • GI: 15928124 NCBI
  • RefSeq: NP_375657 NCBI
  • UniProt:

Protein sequence[edit source | edit]

  • MQSLDKNFRHLSRQQKLQQLVDKQWLSEDQFDILLNHPLIDEEVANSLIENVIAQGALPVGLLPNIIVDDKAYVVPMMVEEPSVVAAASYGAKLVNQTGGFKTVSSERIMIGQIVFDGVDDTEKLSADIKALEKQIHKIADEAYPSIKARGGGYQRIAIDTFPEQQLLSLKVFVDTKDAMGANMLNTILEAITAFLKNESPQSDILMSILSNHATASVVKVQGEIDVKDLARGERTGEEVAKRMERASVLAQVDIHRAATHNKGVMNGIHAVVLATGNDTRGAEASAHAYASRDGQYRGIATWRYDQKRQRLIGTIEVPMTLAIVGGGTKVLPIAKASLELLNVDSAQELGHVVAAVGLAQNFAACRALVSEGIQQGHMSLQYKSLAIVVGAKGDEIAQVAEALKQEPRANTQVAERILQEIRQQ

Experimental data[edit source | edit]

  • experimentally validated: no data available

Expression & Regulation[edit source | edit]

Operon[edit source | edit]

  • mvaA no polycistronic organisation predicted

Regulation[edit source | edit]

  • sigma factor:
  • regulator: CcpA
    CcpA (TF) important in Carbon catabolismRegPrecise

Transcription pattern[edit source | edit]

Protein synthesis (provided by Aureolib)[edit source | edit]

Protein stability[edit source | edit]

  • half-life: no data available

Biological Material[edit source | edit]

Mutants[edit source | edit]

Expression vector[edit source | edit]

lacZ fusion[edit source | edit]

GFP fusion[edit source | edit]

two-hybrid system[edit source | edit]

FLAG-tag construct[edit source | edit]

Antibody[edit source | edit]

Other Information[edit source | edit]

You are kindly invited to share additional interesting facts.

Literature[edit source | edit]

References[edit source | edit]

Relevant publications[edit source | edit]