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PangenomeCOLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 10-JUN-2013

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus COL
  • locus tag: SACOL0321 [new locus tag: SACOL_RS01620 ]
  • pan locus tag?: SAUPAN001421000
  • symbol: SACOL0321
  • pan gene symbol?: —
  • synonym:
  • product: prophage L54a, repressor protein

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SACOL0321 [new locus tag: SACOL_RS01620 ]
  • symbol: SACOL0321
  • product: prophage L54a, repressor protein
  • replicon: chromosome
  • strand: -
  • coordinates: 357357..358076
  • length: 720
  • essential: unknown other strains

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    ATGGATAAAAAAGAATTAGCGAAATTTATAGGCAATAAAATCAGATACTATAGAACCAAA
    TTGAACTTAACTCAAGATCAACTTGGAGAAAAACTCAACACTAAAAAAGCTACTATTTCA
    AATTATGAGACAGGGTACAGAACTCCTAAACAAGATGATTTGTTTGAAATTGCTCATATT
    TTAAATATCAGTATCGATGATTTGTTTCCTACAAGAAATAATAAAAAAAACGACATCACT
    TCCATATACAACAAACTCACACCTCCCCGCCAAGAAAACGTACTTAACTACGCAAATGAG
    CAATTAGATGAACAGAATAAAGTCACTTCTATAGATGAATATAAAGAGTCTAAACTAGTA
    TCGTATATTGCATGTGGTGCAACTGGTGCTGGCATAGGAGAAGAATTATATGATGACATA
    TTGCATGAAGAAGTATTTTTTAAAGAAGACGAAACGCCATCAAATGCTGATTTTTGTATT
    TTAGTTAATGGTGATTCAATGGAACCTATGTTAAAACAAGGAACATACGCTTTTATTAAG
    AAAGAAGATTCTATTAAAGATGGTACAATTGCACTCGTTGTATTAGATGGAGTAAGTCTT
    ATCAAGCGTGTAGATATATGCGAAGACTATATTAATTTGGTATCTCTAAATCCGAAGTAT
    GATGATATCAAAGTCGCTTCGTTTAGTAATATTAAAGTAATGGGCAAAGTTGTATTGTGA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SACOL0321 [new locus tag: SACOL_RS01620 ]
  • symbol: SACOL0321
  • description: prophage L54a, repressor protein
  • length: 239
  • theoretical pI: 4.74333
  • theoretical MW: 27155.7
  • GRAVY: -0.451883

⊟Function[edit | edit source]

  • TIGRFAM:
    Signal transduction Regulatory functions DNA interactions transcriptional regulator, y4mF family (TIGR03070; HMM-score: 32.1)
    putative zinc finger/helix-turn-helix protein, YgiT family (TIGR03830; HMM-score: 28.3)
    Genetic information processing DNA metabolism DNA replication, recombination, and repair repressor LexA (TIGR00498; EC 3.4.21.88; HMM-score: 27.9)
    Signal transduction Regulatory functions DNA interactions repressor LexA (TIGR00498; EC 3.4.21.88; HMM-score: 27.9)
    and 6 more
    Hypothetical proteins Conserved TIGR00270 family protein (TIGR00270; HMM-score: 21.7)
    Metabolism Energy metabolism Biosynthesis and degradation of polysaccharides glucose-1-phosphate adenylyltransferase, GlgD subunit (TIGR02092; EC 2.7.7.27; HMM-score: 20.5)
    signal peptidase I (TIGR02228; EC 3.4.21.89; HMM-score: 15.8)
    Genetic information processing Protein fate Protein and peptide secretion and trafficking signal peptidase I (TIGR02227; EC 3.4.21.89; HMM-score: 13.5)
    Cellular processes Cellular processes Detoxification nickel-type superoxide dismutase maturation protease (TIGR02754; EC 3.4.21.-; HMM-score: 13.2)
    Genetic information processing Protein fate Protein modification and repair nickel-type superoxide dismutase maturation protease (TIGR02754; EC 3.4.21.-; HMM-score: 13.2)
  • TheSEED  :
    • Phage repressor protein cI
  • PFAM:
    HTH (CL0123) HTH_3; Helix-turn-helix (PF01381; HMM-score: 59.3)
    and 7 more
    Peptidase_SF (CL0299) Peptidase_S24; Peptidase S24-like (PF00717; HMM-score: 41.5)
    HTH (CL0123) HTH_19; Helix-turn-helix domain (PF12844; HMM-score: 40.7)
    HTH_31; Helix-turn-helix domain (PF13560; HMM-score: 31.3)
    Pou; Pou domain - N-terminal to homeobox domain (PF00157; HMM-score: 16.1)
    HTH_26; Cro/C1-type HTH DNA-binding domain (PF13443; HMM-score: 14.7)
    no clan defined BNIP2; Bcl2-/adenovirus E1B nineteen kDa-interacting protein 2 (PF12496; HMM-score: 13.7)
    HTH (CL0123) MqsA_antitoxin; Antitoxin component of bacterial toxin-antitoxin system, MqsA (PF15731; HMM-score: 12.2)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 7.5
    • Cytoplasmic Membrane Score: 1.15
    • Cellwall Score: 0.62
    • Extracellular Score: 0.73
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular possibility: 1
    • Signal peptide possibility: -1
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.005387
    • TAT(Tat/SPI): 0.000305
    • LIPO(Sec/SPII): 0.0006
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MDKKELAKFIGNKIRYYRTKLNLTQDQLGEKLNTKKATISNYETGYRTPKQDDLFEIAHILNISIDDLFPTRNNKKNDITSIYNKLTPPRQENVLNYANEQLDEQNKVTSIDEYKESKLVSYIACGATGAGIGEELYDDILHEEVFFKEDETPSNADFCILVNGDSMEPMLKQGTYAFIKKEDSIKDGTIALVVLDGVSLIKRVDICEDYINLVSLNPKYDDIKVASFSNIKVMGKVVL

⊟Experimental data[edit | edit source]

  • experimentally validated: PeptideAtlas
  • protein localization: Cytoplasmic [1] [2] [3] [4]
  • quantitative data / protein copy number per cell: 127 [5]
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator:

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: 7.48 h [6]

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

  1. ↑ Dörte Becher, Kristina Hempel, Susanne Sievers, Daniela Zühlke, Jan Pané-Farré, Andreas Otto, Stephan Fuchs, Dirk Albrecht, Jörg Bernhardt, Susanne Engelmann, Uwe Völker, Jan Maarten van Dijl, Michael Hecker
    A proteomic view of an important human pathogen--towards the quantification of the entire Staphylococcus aureus proteome.
    PLoS One: 2009, 4(12);e8176
    [PubMed:19997597] [WorldCat.org] [DOI] (I e)
  2. ↑ Kristina Hempel, Jan Pané-Farré, Andreas Otto, Susanne Sievers, Michael Hecker, Dörte Becher
    Quantitative cell surface proteome profiling for SigB-dependent protein expression in the human pathogen Staphylococcus aureus via biotinylation approach.
    J Proteome Res: 2010, 9(3);1579-90
    [PubMed:20108986] [WorldCat.org] [DOI] (I p)
  3. ↑ Kristina Hempel, Florian-Alexander Herbst, Martin Moche, Michael Hecker, Dörte Becher
    Quantitative proteomic view on secreted, cell surface-associated, and cytoplasmic proteins of the methicillin-resistant human pathogen Staphylococcus aureus under iron-limited conditions.
    J Proteome Res: 2011, 10(4);1657-66
    [PubMed:21323324] [WorldCat.org] [DOI] (I p)
  4. ↑ Andreas Otto, Jan Maarten van Dijl, Michael Hecker, Dörte Becher
    The Staphylococcus aureus proteome.
    Int J Med Microbiol: 2014, 304(2);110-20
    [PubMed:24439828] [WorldCat.org] [DOI] (I p)
  5. ↑ Daniela Zühlke, Kirsten Dörries, Jörg Bernhardt, Sandra Maaß, Jan Muntel, Volkmar Liebscher, Jan Pané-Farré, Katharina Riedel, Michael Lalk, Uwe Völker, Susanne Engelmann, Dörte Becher, Stephan Fuchs, Michael Hecker
    Costs of life - Dynamics of the protein inventory of Staphylococcus aureus during anaerobiosis.
    Sci Rep: 2016, 6;28172
    [PubMed:27344979] [WorldCat.org] [DOI] (I e)
  6. ↑ Stephan Michalik, Jörg Bernhardt, Andreas Otto, Martin Moche, Dörte Becher, Hanna Meyer, Michael Lalk, Claudia Schurmann, Rabea Schlüter, Holger Kock, Ulf Gerth, Michael Hecker
    Life and death of proteins: a case study of glucose-starved Staphylococcus aureus.
    Mol Cell Proteomics: 2012, 11(9);558-70
    [PubMed:22556279] [WorldCat.org] [DOI] (I p)

⊟Relevant publications[edit | edit source]

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