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04-0298108BA0217611819-9771193COLECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50N315NCTC8325NewmanRF122ST398T0131TCH60TW20USA300_FPR3757USA300_TCH1516VC40
NCBI: 10-JUN-2013

Summary[edit source | edit]

  • organism: Staphylococcus aureus COL
  • locus tag: SACOL0389 [new locus tag: SACOL_RS01955 ]
  • pan locus tag?:
  • symbol: SACOL0389
  • pan gene symbol?:
  • synonym:
  • product: prophage L54a, amidase

Genome View[edit source | edit]

Gene[edit source | edit]

General[edit source | edit]

  • type: CDS
  • locus tag: SACOL0389 [new locus tag: SACOL_RS01955 ]
  • symbol: SACOL0389
  • product: prophage L54a, amidase
  • replicon: chromosome
  • strand: +
  • coordinates: 396062..397516
  • length: 1455
  • essential: unknown

Accession numbers[edit source | edit]

Phenotype[edit source | edit]

  • Share your knowledge and add information here. [edit]

DNA sequence[edit source | edit]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    ATGTTAATGACAAAAAATCAAGCAGAAAAATGGTTTGACAATTCATTAGGGAAACAATTC
    AACCCAGATGGTTGGTATGGATTTCAGTGTTATGATTACGCCAATATGTTCTTTATGTTA
    GCGACAGGCGAAAGGCTGCAAGGTTTATATGCTTATAATATCCCGTTTGATAATAAAGCA
    AAGATTGAAAAATATGGTCAAATAATTAAAAACTATGACAGCTTTTTACCGCAAAAGTTG
    GACATTGTCGTTTTCCCGTCAAAGTATGGTGGCGGAGCTGGACACGTTGAAATTGTTGAG
    AGCGCAAATTTAAATACTTTCACATCATTTGGTCAAAACTGGAACGGTAAAGGTTGGACT
    AATGGCGTTGCGCAACCTGGTTGGGGTCCTGAAACTGTGACAAGACATGTTCATTATTAT
    GACAATCCAATGTATTTTATTAGGTTAAACTTCCCTAACAACTTAAGCGTTGGCAATAAA
    GCTAAAGGTATTATTAAGCAAGCGACTACAAAAAAAGAGGCAGTAATTAAACCTAAAAAA
    ATTATGCTTGTAGCCGGTCATGGTTATAACGATCCTGGAGCAGTAGGAAACGGAACAAAC
    GAACGCGATTTTATACGTAAATATATAACGCCTAATATCGCTAAGTATTTAAGACATGCA
    GGACATGAAGTTGCATTATACGGTGGCTCAAGTCAATCACAAGATATGTATCAAGATACT
    GCATACGGTGTTAATGTAGGCAATAAAAAAGATTATGGCTTATATTGGGTTAAATCACAG
    GGGTATGACATTGTTCTAGAAATACATTTAGACGCAGCAGGAGAAAGCGCAAGTGGTGGG
    CATGTTATTATCTCAAGTCAATTCAATGCAGATACTATTGATAAAAGTATACAAGATGTT
    ATTAAAAATAACTTAGGACAAATAAGAGGTGTGACACCTCGTAATGATTTACTAAATGTT
    AATGTATCAGCAGAAATAAATATAAATTATCGTTTATCTGAATTAGGTTTTATTACTAAT
    AAAAATGATATGGATTGGATTAAGAAAAACTATGACTTGTATTCTAAATTAATAGCCGGT
    GCGATTCATGGTAAGCCTATAGGTGGTTTGGTAGCTGGTAATGTTAAAACATCAGCTAAA
    AACCAAAAAAATCCACCAGTGCCAGCAGGTTATACACTCGATAAGAATAATGTCCCTTAT
    AAAAAAGAACAAGGCAATTACACAGTAGCTAATGTTAAAGGTAATAATGTAAGAGACGGT
    TATTCAACTAATTCAAGAATTACAGGGGTATTACCCAACAACACAACAATTACGTATGAC
    GGTGCATATTGTATTAATGGTTATAGATGGATTACTTATATTGCTAATAGTGGACAACGT
    CGTTATATAGCGACAGGAGAGGTAGACAAGGCAGGTAATAGAATAAGTAGTTTTGGTAAG
    TTTAGCACGATTTAG
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1200
    1260
    1320
    1380
    1440
    1455

Protein[edit source | edit]

General[edit source | edit]

  • locus tag: SACOL0389 [new locus tag: SACOL_RS01955 ]
  • symbol: SACOL0389
  • description: prophage L54a, amidase
  • length: 484
  • theoretical pI: 9.66172
  • theoretical MW: 53800.2
  • GRAVY: -0.508884

Function[edit source | edit]

  • reaction:
  • TIGRFAM:
    Cell structureCell envelopeBiosynthesis and degradation of murein sacculus and peptidoglycanN-acetylmuramoyl-L-alanine amidase CwlD (TIGR02883; EC 3.5.1.28; HMM-score: 43.8)
    Cellular processesCellular processesSporulation and germinationN-acetylmuramoyl-L-alanine amidase CwlD (TIGR02883; EC 3.5.1.28; HMM-score: 43.8)
  • TheSEED:  
    Phage N-acetylmuramoyl-L-alanine amidase (EC 3.5.1.28) 
  • PFAM:
    Peptidase_MH (CL0035) Amidase_3; N-acetylmuramoyl-L-alanine amidase (PF01520; HMM-score: 136.8)
    Peptidase_CA (CL0125) CHAP; CHAP domain (PF05257; HMM-score: 37.5)
    SH3 (CL0010) SH3_5; Bacterial SH3 domain (PF08460; HMM-score: 35.6)
    SH3_3; Bacterial SH3 domain (PF08239; HMM-score: 17.2)

Structure, modifications & interactions[edit source | edit]

  • domains:
  • modifications:
  • cofactors:
  • effectors:
  • protein partners:

Localization[edit source | edit]

  • PSORTb: unknown (no significant prediction)
    • Cytoplasmic Score: 2.5
    • Cytoplasmic Membrane Score: 2.5
    • Cellwall Score: 2.5
    • Extracellular Score: 2.5
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular Possibility: 1
    • Signal Peptide Possibility: -1
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • Ymax: 0.131
    • Ymax_pos: 11
    • Cmax: 0.115
    • Cmax_pos: 52
    • Smax: 0.262
    • Smax_pos: 6
    • Smean: 0.165
    • D: 0.144
  • predicted transmembrane helices (TMHMM): 0

Accession numbers[edit source | edit]

Protein sequence[edit source | edit]

  • MLMTKNQAEKWFDNSLGKQFNPDGWYGFQCYDYANMFFMLATGERLQGLYAYNIPFDNKAKIEKYGQIIKNYDSFLPQKLDIVVFPSKYGGGAGHVEIVESANLNTFTSFGQNWNGKGWTNGVAQPGWGPETVTRHVHYYDNPMYFIRLNFPNNLSVGNKAKGIIKQATTKKEAVIKPKKIMLVAGHGYNDPGAVGNGTNERDFIRKYITPNIAKYLRHAGHEVALYGGSSQSQDMYQDTAYGVNVGNKKDYGLYWVKSQGYDIVLEIHLDAAGESASGGHVIISSQFNADTIDKSIQDVIKNNLGQIRGVTPRNDLLNVNVSAEININYRLSELGFITNKNDMDWIKKNYDLYSKLIAGAIHGKPIGGLVAGNVKTSAKNQKNPPVPAGYTLDKNNVPYKKEQGNYTVANVKGNNVRDGYSTNSRITGVLPNNTTITYDGAYCINGYRWITYIANSGQRRYIATGEVDKAGNRISSFGKFSTI

Experimental data[edit source | edit]

  • experimental localization: Cytoplasmic [1] [2]

Expression & Regulation[edit source | edit]

Operon[edit source | edit]

Regulation[edit source | edit]

  • sigma factor:
  • regulator:

Transcription pattern[edit source | edit]

Protein synthesis (provided by Aureolib)[edit source | edit]

Protein stability[edit source | edit]

  • half-life: no data available

Biological Material[edit source | edit]

Mutants[edit source | edit]

Expression vector[edit source | edit]

lacZ fusion[edit source | edit]

GFP fusion[edit source | edit]

two-hybrid system[edit source | edit]

FLAG-tag construct[edit source | edit]

Antibody[edit source | edit]

Other Information[edit source | edit]

You are kindly invited to share additional interesting facts.

Literature[edit source | edit]

References[edit source | edit]

  1. Kristina Hempel, Florian-Alexander Herbst, Martin Moche, Michael Hecker, Dörte Becher
    Quantitative proteomic view on secreted, cell surface-associated, and cytoplasmic proteins of the methicillin-resistant human pathogen Staphylococcus aureus under iron-limited conditions.
    J. Proteome Res.: 2011, 10(4);1657-66
    [PubMed:21323324] [WorldCat.org] [DOI] (I p)
  2. Andreas Otto, Jan Maarten van Dijl, Michael Hecker, Dörte Becher
    The Staphylococcus aureus proteome.
    Int. J. Med. Microbiol.: 2014, 304(2);110-20
    [PubMed:24439828] [WorldCat.org] [DOI] (I p)

Relevant publications[edit source | edit]