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NCBI: 10-JUN-2013

Summary[edit source | edit]

  • organism: Staphylococcus aureus COL
  • locus tag: SACOL0407 [new locus tag: SACOL_RS02050 ]
  • pan locus tag?: SAUPAN001872000
  • symbol: glpT
  • pan gene symbol?: glpT
  • synonym:
  • product: glycerol-3-phosphate transporter

Genome View[edit source | edit]

Gene[edit source | edit]

General[edit source | edit]

  • type: CDS
  • locus tag: SACOL0407 [new locus tag: SACOL_RS02050 ]
  • symbol: glpT
  • product: glycerol-3-phosphate transporter
  • replicon: chromosome
  • strand: -
  • coordinates: 413356..414714
  • length: 1359
  • essential: unknown other strains

Accession numbers[edit source | edit]

Phenotype[edit source | edit]

  • Share your knowledge and add information here. [edit]

DNA sequence[edit source | edit]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    ATGAATTTTCTTAAACCTGCAAAGCATATTAAGCCTTTGCCAGAAAATCAGATAGATGAT
    ACCTATAAACGATTACGTCTCCAAGTATTTCTTGGTATTTTCATCGGTTACGCTGGGTAC
    TATTTATTACGTAAAAACTTTTCGTTAGCGATGCCGGCATTGCAAGAGCAAGGTTTTACA
    AAAGCGGAACTAGGTTTTGCACTTTCTGCTGTTTCCATCGCATATGGATTTAGTAAGTTC
    TTTATGGGTACTGTAAGTGATCGGAGCAATGCTCGGATATTCTTAGTTCTTGGATTAGCA
    CTCACTGCTATCGTCAATTTGTTAATGGGATTTGTACCGTTCTTTACATCAGGTATCGGT
    ATTATGTTTGTCCTATTATTCTTAAATGGATGGTTTCAAGGTATGGGCTGGCCACCTTCA
    GGCCGTGTTCTCGTTCACTGGTTTAGTGTAAGTGAACGCGGAAGTAAGACTGCCCTTTGG
    AACGTTGCGCATAATGTTGGTGGAGGTATTATGGCACCTATTGCTGCTTGGGGTATTACA
    ACAACTGCATTTATCAACTTTGGTTATTTAAAAGGTTTCGAAGGTGTATTCATTTACCCT
    GCACTCTTAGCACTTATCATTGCCGCAATTTCATACGTATTGATTAGAGACACACCTCAA
    TCTCAAGGTTTACCTCCAATCGAAATTTATAAAAATGACTTTGCTACAAGCGATAAGAAA
    ACATTAGAAACAGAATTAACTACAAAAGAAATTTTATTTAAATATGTACTGAACAATAAA
    TGGGTATGGGCAATTGCCTTTGCAAATATATTTGTTTATTTCGTGCGTTATGGTGTACTT
    GATTGGGCGCCAGTCTACTTAAGTGAAGAAAAACATTTCGACTTAAAAGCATCAGGTTGG
    GCATACTTCTTATACGAATGGGCTGGAATTCCTGGTACATTATTATGTGGTTACATTTCT
    GATAAATTATTCAAAGGTCGTCGTGGACCTGCAGGTTTCTTCTTTATGTTAGGTGTCACA
    GTATTTGTATTAATTTATTGGTTAAATCCTCCAGGCAATGCTTGGTTAGACAATGTCTCA
    TTAATTGCCATTGGTTTCTTAATATATGGACCAGTTATGTTAATTGGTTTACAAGCATTA
    GATTATGTACCTAAAAAAGCAGCTGGCACAGCAGCTGGATTAACAGGATTATTTGGTTAT
    CTGTTTGGTGCTGTAATGGCCAACATCGTCTTAGGTGCTGTAGTTGATAAATTCGGATGG
    GATGTCGGTTTTATTTTATTAACAGCAATTAGTGTGTTTGCAATGTTGAGCTTTATCCTC
    ACTTGGAATAAAGTAGGACAAGAAACCGTTCATCATTAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1200
    1260
    1320
    1359

Protein[edit source | edit]

General[edit source | edit]

  • locus tag: SACOL0407 [new locus tag: SACOL_RS02050 ]
  • symbol: GlpT
  • description: glycerol-3-phosphate transporter
  • length: 452
  • theoretical pI: 9.59874
  • theoretical MW: 50167.7
  • GRAVY: 0.568363

Function[edit source | edit]

  • reaction:
  • TIGRFAM:
    MetabolismTransport and binding proteinsOtherglycerol-3-phosphate transporter (TIGR00712; HMM-score: 666.5)
    MetabolismTransport and binding proteinsCarbohydrates, organic alcohols, and acidsphosphoglycerate transporter family protein (TIGR00881; HMM-score: 467.2)
    MetabolismTransport and binding proteinsCarbohydrates, organic alcohols, and acidsD-galactonate transporter (TIGR00893; HMM-score: 128)
    MetabolismTransport and binding proteinsAnionsNa(+)-dependent inorganic phosphate cotransporter (TIGR00894; HMM-score: 56.7)
    MetabolismTransport and binding proteinsCarbohydrates, organic alcohols, and acidsMFS transporter, aromatic acid:H+ symporter (AAHS) family (TIGR00895; HMM-score: 46.2)
    MetabolismTransport and binding proteinsUnknown substrateMFS transporter, metabolite:H+ symporter (MHS) family protein (TIGR00883; HMM-score: 44.4)
    MetabolismTransport and binding proteinsCarbohydrates, organic alcohols, and acidsoxalate/formate antiporter family transporter (TIGR00890; HMM-score: 26.5)
    MetabolismTransport and binding proteinsCarbohydrates, organic alcohols, and acidsMFS transporter, sialate:H+ symporter (SHS) family (TIGR00891; HMM-score: 22.1)
    multidrug resistance protein (TIGR00880; HMM-score: 16.2)
    Cellular processesCellular processesPathogenesistype III secretion protein, HrcV family (TIGR01399; HMM-score: 9)
    Genetic information processingProtein fateProtein and peptide secretion and traffickingtype III secretion protein, HrcV family (TIGR01399; HMM-score: 9)
  • TheSEED:  
    CarbohydratesSugar alcoholsGlycerol and Glycerol-3-phosphate Uptake and Utilization Glycerol-3-phosphate transporter 
  • PFAM:
    MFS (CL0015) MFS_1; Major Facilitator Superfamily (PF07690; HMM-score: 162.4)
    OATP; Organic Anion Transporter Polypeptide (OATP) family (PF03137; HMM-score: 9.6)

Structure, modifications & interactions[edit source | edit]

  • domains:
  • modifications:
  • cofactors:
  • effectors:
  • protein partners:

Localization[edit source | edit]

  • PSORTb: Cytoplasmic Membrane
    • Cytoplasmic Score: 0
    • Cytoplasmic Membrane Score: 10
    • Cellwall Score: 0
    • Extracellular Score: 0
    • Internal Helices: 12
  • LocateP: Multi-transmembrane
    • Prediction by SwissProt Classification: Membrane
    • Pathway Prediction: Sec-(SPI)
    • Intracellular Possibility: 0.17
    • Signal Peptide Possibility: -1
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • Ymax: 0.12
    • Ymax_pos: 11
    • Cmax: 0.144
    • Cmax_pos: 51
    • Smax: 0.211
    • Smax_pos: 1
    • Smean: 0.126
    • D: 0.122
  • predicted transmembrane helices (TMHMM): 12

Accession numbers[edit source | edit]

Protein sequence[edit source | edit]

  • MNFLKPAKHIKPLPENQIDDTYKRLRLQVFLGIFIGYAGYYLLRKNFSLAMPALQEQGFTKAELGFALSAVSIAYGFSKFFMGTVSDRSNARIFLVLGLALTAIVNLLMGFVPFFTSGIGIMFVLLFLNGWFQGMGWPPSGRVLVHWFSVSERGSKTALWNVAHNVGGGIMAPIAAWGITTTAFINFGYLKGFEGVFIYPALLALIIAAISYVLIRDTPQSQGLPPIEIYKNDFATSDKKTLETELTTKEILFKYVLNNKWVWAIAFANIFVYFVRYGVLDWAPVYLSEEKHFDLKASGWAYFLYEWAGIPGTLLCGYISDKLFKGRRGPAGFFFMLGVTVFVLIYWLNPPGNAWLDNVSLIAIGFLIYGPVMLIGLQALDYVPKKAAGTAAGLTGLFGYLFGAVMANIVLGAVVDKFGWDVGFILLTAISVFAMLSFILTWNKVGQETVHH

Experimental data[edit source | edit]

  • experimental localization: Integral membrane [1] [2]

Expression & Regulation[edit source | edit]

Operon[edit source | edit]

  • glpT no polycistronic organisation predicted

Regulation[edit source | edit]

  • sigma factor:
  • regulator:

Transcription pattern[edit source | edit]

Protein synthesis (provided by Aureolib)[edit source | edit]

Protein stability[edit source | edit]

  • half-life: no data available

Biological Material[edit source | edit]

Mutants[edit source | edit]

Expression vector[edit source | edit]

lacZ fusion[edit source | edit]

GFP fusion[edit source | edit]

two-hybrid system[edit source | edit]

FLAG-tag construct[edit source | edit]

Antibody[edit source | edit]

Other Information[edit source | edit]

You are kindly invited to share additional interesting facts.

Literature[edit source | edit]

References[edit source | edit]

  1. Dörte Becher, Kristina Hempel, Susanne Sievers, Daniela Zühlke, Jan Pané-Farré, Andreas Otto, Stephan Fuchs, Dirk Albrecht, Jörg Bernhardt, Susanne Engelmann, Uwe Völker, Jan Maarten van Dijl, Michael Hecker
    A proteomic view of an important human pathogen--towards the quantification of the entire Staphylococcus aureus proteome.
    PLoS ONE: 2009, 4(12);e8176
    [PubMed:19997597] [WorldCat.org] [DOI] (I e)
  2. Andreas Otto, Jan Maarten van Dijl, Michael Hecker, Dörte Becher
    The Staphylococcus aureus proteome.
    Int. J. Med. Microbiol.: 2014, 304(2);110-20
    [PubMed:24439828] [WorldCat.org] [DOI] (I p)

Relevant publications[edit source | edit]