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NCBI: 10-JUN-2013

Summary[edit source | edit]

  • organism: Staphylococcus aureus COL
  • locus tag: SACOL0428 [new locus tag: SACOL_RS02155 ]
  • pan locus tag?: SAUPAN001900000
  • symbol: metE
  • pan gene symbol?: metE
  • synonym:
  • product: 5- methyltetrahydropteroyltriglutamate/homocysteine S-methyltransferase

Genome View[edit source | edit]

Gene[edit source | edit]

General[edit source | edit]

  • type: CDS
  • locus tag: SACOL0428 [new locus tag: SACOL_RS02155 ]
  • symbol: metE
  • product: 5- methyltetrahydropteroyltriglutamate/homocysteine S-methyltransferase
  • replicon: chromosome
  • strand: -
  • coordinates: 431989..434217
  • length: 2229
  • essential: unknown other strains

Accession numbers[edit source | edit]

Phenotype[edit source | edit]

  • Share your knowledge and add information here. [edit]

DNA sequence[edit source | edit]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    ATGACAACAATTAAAACATCAAACTTAGGATTCCCAAGATTAGGTAGAAAAAGAGAATGG
    AAAAAAGCCATCGAAAGTTATTGGGCAAAGAAGATTTCTAAAGAAGAATTAGATCAAACA
    TTAACTGATCTACATAAAGAAAATTTATTATTACAAAAGTACTATCACTTAGATAGCATC
    CCAGTTGGTGATTTCTCTTTATATGACCATATATTAGATACGTCATTATTATTCAACATC
    ATCCCTGAACGTTTCCAAGGAAGAACTATTGATGATGATTTATTATTTGATATTGCTCGC
    GGTAATAAAGACCATGTTGCAAGTGCACTTATAAAATGGTTTAACACTAACTATCATTAC
    ATCGTACCTGAATGGGACAATGTTGAGCCTAAAGTGAGTCGCAATGTATTATTAGATCGC
    TTTAAATATGCCCAATCTTTAAACGTTAACGCTCACCCTGTCATTGTTGGTCCGATTACT
    TTTGTTAAATTATCAAAAGGCGGTCACCAAACTTTTGAAGAAAAAGTTAAAACGTTATTA
    CCACTTTATAAAGAAGTGTTTGAATCATTAATTGATGCAGGTGCTGAGTACATTCAAGTT
    GATGAGCCAATCTTAGTTACAGACGACAGCGAAAGCTATGAAAATATTACACGTGAAGCT
    TATGACTATTTCGAAAAAGCTGGTGTTGCTAAAAAATTAGTCATTCAAACATACTTTGAA
    CGTGCACATCTTAAATTCTTAAGTTCTTTACCTGTTGGTGGTATAGGTTTAGACTTTGTC
    CATGATAATGGCTATAACCTTAAACAAATTGAAGCTGGAGATTTTGATAAATCAAAAACA
    TTATACGCTGGAATTATTGATGGTCGTAATGTATGGGCAAGTGACATTGAAGCTAAAAAA
    GTCTTAATCGATAAATTGTTAGCACACACTAATGAACTTGTCATTCAACCATCATCTTCA
    TTATTACATGTTCCAGTATCTTTAGATGATGAAACATTAGATACAAGTGTTGGAGAAGGC
    TTAAGCTTTGCAACTGAAAAATTAGACGAATTAGATGCATTGCGTCGCCTATTTAATCAA
    AACGATAGTGTTAAATATGATAAATTAAAAGCACGTTATGAGCGTTTCCAAAATCAATCA
    TTCAAAAACTTAGATTATGATTTCGAAAGCGTGCGTACTTCAAGACAATCACCATTCGCG
    CAACGTATTGAACAACAACAAAAACGTTTGAACTTACCGGATTTACCAACAACAACTATT
    GGATCATTCCCACAAAGCCGAGAAGTTCGAAAATACCGTGCAGATTGGAAGAACAAACGC
    ATTACAGACGAAGCATATGAAACATTCTTAAAAAATGAAATTGCTCGATGGATTAAAATT
    CAAGAAGACATTGGCTTAGATGTATTAGTTCACGGTGAATTTGAACGTAATGACATGGTT
    GAATTCTTCGGAGAAAAATTACAAGGTTTCTTAGTAACTAAATTCGGTTGGGTGCAATCA
    TATGGTTCACGTGCCGTAAAACCACCAATCATTTATGGTGATGTAAAATGGACAGCGCCT
    TTAACTGTTGATGAAACAGTTTATGCACAAAGCTTAACAGATAAACCAGTTAAAGGTATG
    TTAACTGGACCTGTAACAATTCTAAACTGGTCATTTGAACGTGTTGATTTACCACGTAAA
    GTCGTTCAAGATCAAATTGCTTTAGCAATCAACGAAGAAGTATTAGCACTTGAAGCTGCA
    GGAATCAAAGTTATCCAAGTTGACGAACCTGCATTACGTGAAGGCTTACCATTACGCTCT
    GAATATCACGAACAATATCTTAAAGATGCTGTTTTATCATTTAAACTTGCAACGTCTTCA
    GTTCGTGATGAAACTCAAATCCATACACATATGTGTTATTCTCAATTCGGTCAAATCATT
    CATGCTATTCATGACTTAGATGCTGATGTTATTTCAATTGAAACATCTCGTAGCCATGGT
    GATTTAATTAAAGACTTTGAAGATATTAATTATGATTTAGGTATTGGTCTAGGTGTATAT
    GATATTCATAGTCCACGTATTCCAACAAAAGAAGAAATTACTACAGCAATCAATCGTTCA
    CTACAACAAATCGACCGCTCATTATTCTGGGTAAACCCTGACTGTGGTTTAAAAACGCGA
    AAAGAAGAAGAAGTTAAAGATGCATTGACTGTGCTTGTGAATGCTGTTAAAGCTAAACGC
    CAAGAATAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1200
    1260
    1320
    1380
    1440
    1500
    1560
    1620
    1680
    1740
    1800
    1860
    1920
    1980
    2040
    2100
    2160
    2220
    2229

Protein[edit source | edit]

General[edit source | edit]

  • locus tag: SACOL0428 [new locus tag: SACOL_RS02155 ]
  • symbol: MetE
  • description: 5- methyltetrahydropteroyltriglutamate/homocysteine S-methyltransferase
  • length: 742
  • theoretical pI: 5.40741
  • theoretical MW: 85074.8
  • GRAVY: -0.39434

Function[edit source | edit]

  • reaction:
    EC 2.1.1.14?  ExPASy
    5-methyltetrahydropteroyltriglutamate--homocysteine S-methyltransferase5-methyltetrahydropteroyltri-L-glutamate + L-homocysteine = tetrahydropteroyltri-L-glutamate + L-methionine
  • TIGRFAM:
    MetabolismAmino acid biosynthesisAspartate family5-methyltetrahydropteroyltriglutamate--homocysteine S-methyltransferase (TIGR01371; EC 2.1.1.14; HMM-score: 1093.7)
    conserved hypothetical protein (TIGR04540; HMM-score: 14.2)
  • TheSEED:  
    Amino Acids and DerivativesLysine, threonine, methionine, and cysteineMethionine Biosynthesis 5-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase (EC 2.1.1.14) 
  • PFAM:
    Methionine_synt (CL0160) Meth_synt_2; Cobalamin-independent synthase, Catalytic domain (PF01717; HMM-score: 473.3)
    Meth_synt_1; Cobalamin-independent synthase, N-terminal domain (PF08267; HMM-score: 389.1)
    URO-D; Uroporphyrinogen decarboxylase (URO-D) (PF01208; HMM-score: 20)
    T3SS-Chaperone (CL0419) FliJ; Flagellar FliJ protein (PF02050; HMM-score: 8)

Structure, modifications & interactions[edit source | edit]

  • domains:
  • modifications:
  • cofactors: Zn2+
  • effectors:
  • protein partners:

Localization[edit source | edit]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 7.5
    • Cytoplasmic Membrane Score: 1.15
    • Cellwall Score: 0.62
    • Extracellular Score: 0.73
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular Possibility: 1
    • Signal Peptide Possibility: -1
    • N-terminally Anchored Score: -1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • Ymax: 0.14
    • Ymax_pos: 16
    • Cmax: 0.105
    • Cmax_pos: 24
    • Smax: 0.267
    • Smax_pos: 6
    • Smean: 0.187
    • D: 0.158
  • predicted transmembrane helices (TMHMM): 0

Accession numbers[edit source | edit]

Protein sequence[edit source | edit]

  • MTTIKTSNLGFPRLGRKREWKKAIESYWAKKISKEELDQTLTDLHKENLLLQKYYHLDSIPVGDFSLYDHILDTSLLFNIIPERFQGRTIDDDLLFDIARGNKDHVASALIKWFNTNYHYIVPEWDNVEPKVSRNVLLDRFKYAQSLNVNAHPVIVGPITFVKLSKGGHQTFEEKVKTLLPLYKEVFESLIDAGAEYIQVDEPILVTDDSESYENITREAYDYFEKAGVAKKLVIQTYFERAHLKFLSSLPVGGIGLDFVHDNGYNLKQIEAGDFDKSKTLYAGIIDGRNVWASDIEAKKVLIDKLLAHTNELVIQPSSSLLHVPVSLDDETLDTSVGEGLSFATEKLDELDALRRLFNQNDSVKYDKLKARYERFQNQSFKNLDYDFESVRTSRQSPFAQRIEQQQKRLNLPDLPTTTIGSFPQSREVRKYRADWKNKRITDEAYETFLKNEIARWIKIQEDIGLDVLVHGEFERNDMVEFFGEKLQGFLVTKFGWVQSYGSRAVKPPIIYGDVKWTAPLTVDETVYAQSLTDKPVKGMLTGPVTILNWSFERVDLPRKVVQDQIALAINEEVLALEAAGIKVIQVDEPALREGLPLRSEYHEQYLKDAVLSFKLATSSVRDETQIHTHMCYSQFGQIIHAIHDLDADVISIETSRSHGDLIKDFEDINYDLGIGLGVYDIHSPRIPTKEEITTAINRSLQQIDRSLFWVNPDCGLKTRKEEEVKDALTVLVNAVKAKRQE

Experimental data[edit source | edit]

  • experimentally validated: PeptideAtlas
    experimental localization: Cytoplasmic [1] [2] [3]
    quantitative data / protein copy number per cell: 18051 [4]

Expression & Regulation[edit source | edit]

Operon[edit source | edit]

Regulation[edit source | edit]

  • sigma factor:
  • regulator:

Transcription pattern[edit source | edit]

Protein synthesis (provided by Aureolib)[edit source | edit]

Protein stability[edit source | edit]

  • half-life: 35.21 h [5]

Biological Material[edit source | edit]

Mutants[edit source | edit]

Expression vector[edit source | edit]

lacZ fusion[edit source | edit]

GFP fusion[edit source | edit]

two-hybrid system[edit source | edit]

FLAG-tag construct[edit source | edit]

Antibody[edit source | edit]

Other Information[edit source | edit]

You are kindly invited to share additional interesting facts.

Literature[edit source | edit]

References[edit source | edit]

  1. Dörte Becher, Kristina Hempel, Susanne Sievers, Daniela Zühlke, Jan Pané-Farré, Andreas Otto, Stephan Fuchs, Dirk Albrecht, Jörg Bernhardt, Susanne Engelmann, Uwe Völker, Jan Maarten van Dijl, Michael Hecker
    A proteomic view of an important human pathogen--towards the quantification of the entire Staphylococcus aureus proteome.
    PLoS ONE: 2009, 4(12);e8176
    [PubMed:19997597] [WorldCat.org] [DOI] (I e)
  2. Kristina Hempel, Florian-Alexander Herbst, Martin Moche, Michael Hecker, Dörte Becher
    Quantitative proteomic view on secreted, cell surface-associated, and cytoplasmic proteins of the methicillin-resistant human pathogen Staphylococcus aureus under iron-limited conditions.
    J. Proteome Res.: 2011, 10(4);1657-66
    [PubMed:21323324] [WorldCat.org] [DOI] (I p)
  3. Andreas Otto, Jan Maarten van Dijl, Michael Hecker, Dörte Becher
    The Staphylococcus aureus proteome.
    Int. J. Med. Microbiol.: 2014, 304(2);110-20
    [PubMed:24439828] [WorldCat.org] [DOI] (I p)
  4. Daniela Zühlke, Kirsten Dörries, Jörg Bernhardt, Sandra Maaß, Jan Muntel, Volkmar Liebscher, Jan Pané-Farré, Katharina Riedel, Michael Lalk, Uwe Völker, Susanne Engelmann, Dörte Becher, Stephan Fuchs, Michael Hecker
    Costs of life - Dynamics of the protein inventory of Staphylococcus aureus during anaerobiosis.
    Sci Rep: 2016, 6;28172
    [PubMed:27344979] [WorldCat.org] [DOI] (I e)
  5. Stephan Michalik, Jörg Bernhardt, Andreas Otto, Martin Moche, Dörte Becher, Hanna Meyer, Michael Lalk, Claudia Schurmann, Rabea Schlüter, Holger Kock, Ulf Gerth, Michael Hecker
    Life and death of proteins: a case study of glucose-starved Staphylococcus aureus.
    Mol. Cell Proteomics: 2012, 11(9);558-70
    [PubMed:22556279] [WorldCat.org] [DOI] (I p)

Relevant publications[edit source | edit]