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Pangenome04-0298108BA0217611819-9771193COLECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50N315NCTC8325NewmanRF122ST398T0131TCH60TW20USA300_FPR3757USA300_TCH1516VC40
NCBI: 10-JUN-2013

Summary[edit source | edit]

  • organism: Staphylococcus aureus COL
  • locus tag: SACOL0646 [new locus tag: SACOL_RS03335 ]
  • pan locus tag?: SAUPAN002447000
  • symbol: SACOL0646
  • pan gene symbol?:
  • synonym:
  • product: hypothetical protein

Genome View[edit source | edit]

Gene[edit source | edit]

General[edit source | edit]

  • type: CDS
  • locus tag: SACOL0646 [new locus tag: SACOL_RS03335 ]
  • symbol: SACOL0646
  • product: hypothetical protein
  • replicon: chromosome
  • strand: +
  • coordinates: 676995..677612
  • length: 618
  • essential: unknown other strains

Accession numbers[edit source | edit]

Phenotype[edit source | edit]

  • Share your knowledge and add information here. [edit]

DNA sequence[edit source | edit]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    TTGCTTTGTGAAACCGAAATTATCAATAAAAATCCGAAATACAGAGTTATTAAGTATGAT
    GATGAGTATTTAATGGTCGATGTAATAAGAACTTGGCTTGTATACTTTTTTCCATTTATT
    AATTGGTTCATTCCAAAAAGGTGCGCCAAAATTAGTAGAGAAGAGTATGAAAAACTGAAC
    ACTGTTAAGCCAGTTAAAAATAAAAATTTTTGGCCTGTTGTAGGAGGAACAATCCTACTA
    GGTGCTACTTCCAGAAAGTACATACACTTACTTAATATTCAATTAGAAAAAAGATCGGTT
    ATATTTATATGCTTCGTTGTATTTCTATGTATTTTAATCTTTTTTGTGCTTCTGAATCGA
    AAATTAAAATTGAAAGTTTTCGATAATAAAAAAGAAGAACAAAAAATAATTTTAGTACCA
    ACATTAAAAAATGCTGTTCTTATATTATATGGCTATTTGTTGATTGGAGGCATGTCAATA
    TTAGCTTTAAGCATGTTGCTGACATTAGAAAACCAGAATTTAATAACTTTTATAGCTTGG
    GGTATGGGGTTAATGTTGTTTTTCTTGATGAATATAACACTCATTGTCAATAAAACAGTC
    AAAGTTATAAAAAGGTGA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    618

Protein[edit source | edit]

General[edit source | edit]

  • locus tag: SACOL0646 [new locus tag: SACOL_RS03335 ]
  • symbol: SACOL0646
  • description: hypothetical protein
  • length: 205
  • theoretical pI: 10.306
  • theoretical MW: 24120.3
  • GRAVY: 0.514634

Function[edit source | edit]

  • reaction:
  • TIGRFAM:
    tandem five-TM protein (TIGR01218; HMM-score: 171.3)
  • TheSEED:  
    repetative hypothetical protein , fig|282458.1.peg.574 homolog 
  • PFAM:
    no clan definedDUF443; Protein of unknown function (DUF443) (PF04276; HMM-score: 202.8)
    LysE (CL0292) TauE; Sulfite exporter TauE/SafE (PF01925; HMM-score: 22.6)
    no clan definedDHHC; DHHC palmitoyltransferase (PF01529; HMM-score: 10.6)
    DUF4131; Domain of unknown function (DUF4131) (PF13567; HMM-score: 6)

Structure, modifications & interactions[edit source | edit]

  • domains:
  • modifications:
  • cofactors:
  • effectors:
  • protein partners:

Localization[edit source | edit]

  • PSORTb: Cytoplasmic Membrane
    • Cytoplasmic Score: 0
    • Cytoplasmic Membrane Score: 10
    • Cellwall Score: 0
    • Extracellular Score: 0
    • Internal Helices: 4
  • LocateP: Multi-transmembrane
    • Prediction by SwissProt Classification: Membrane
    • Pathway Prediction: Sec-(SPI)
    • Intracellular Possibility: 0.17
    • Signal Peptide Possibility: -1
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • Ymax: 0.104
    • Ymax_pos: 52
    • Cmax: 0.121
    • Cmax_pos: 52
    • Smax: 0.133
    • Smax_pos: 49
    • Smean: 0.066
    • D: 0.089
  • predicted transmembrane helices (TMHMM): 5

Accession numbers[edit source | edit]

Protein sequence[edit source | edit]

  • MLCETEIINKNPKYRVIKYDDEYLMVDVIRTWLVYFFPFINWFIPKRCAKISREEYEKLNTVKPVKNKNFWPVVGGTILLGATSRKYIHLLNIQLEKRSVIFICFVVFLCILIFFVLLNRKLKLKVFDNKKEEQKIILVPTLKNAVLILYGYLLIGGMSILALSMLLTLENQNLITFIAWGMGLMLFFLMNITLIVNKTVKVIKR

Experimental data[edit source | edit]

  • experimentally validated: no data available

Expression & Regulation[edit source | edit]

Operon[edit source | edit]

Regulation[edit source | edit]

  • sigma factor:
  • regulator:

Transcription pattern[edit source | edit]

Protein synthesis (provided by Aureolib)[edit source | edit]

Protein stability[edit source | edit]

  • half-life: no data available

Biological Material[edit source | edit]

Mutants[edit source | edit]

Expression vector[edit source | edit]

lacZ fusion[edit source | edit]

GFP fusion[edit source | edit]

two-hybrid system[edit source | edit]

FLAG-tag construct[edit source | edit]

Antibody[edit source | edit]

Other Information[edit source | edit]

You are kindly invited to share additional interesting facts.

Literature[edit source | edit]

References[edit source | edit]

Relevant publications[edit source | edit]