From AureoWiki
Jump to: navigation, search
NCBI: 10-JUN-2013

Summary[edit source | edit]

  • organism: Staphylococcus aureus COL
  • locus tag: SACOL0801 [new locus tag: SACOL_RS04120 ]
  • pan locus tag?: SAUPAN002642000
  • symbol: murB
  • pan gene symbol?: murB
  • synonym:
  • product: UDP-N-acetylenolpyruvoylglucosamine reductase

Genome View[edit source | edit]

Gene[edit source | edit]

General[edit source | edit]

  • type: CDS
  • locus tag: SACOL0801 [new locus tag: SACOL_RS04120 ]
  • symbol: murB
  • product: UDP-N-acetylenolpyruvoylglucosamine reductase
  • replicon: chromosome
  • strand: -
  • coordinates: 824105..825028
  • length: 924
  • essential: unknown other strains

Accession numbers[edit source | edit]

Phenotype[edit source | edit]

  • Share your knowledge and add information here. [edit]

DNA sequence[edit source | edit]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    GTGATAAATAAAGACATCTATCAAGCTTTACAACAACTTATCCCAAATGAAAAAATTAAA
    GTTGATGAACCTTTAAAACGATACACTTATACTAAAACAGGTGGTAATGCCGACTTTTAC
    ATTACCCCTACTAAAAATGAAGAAGTACAAGCAGTTGTTAAATATGCCTATCAAAATGAG
    ATTCCTGTTACATATTTAGGAAATGGCTCAAATATTATTATCCGTGAAGGTGGTATTCGC
    GGTATTGTAATTAGTTTATTATCACTAGATCATATCGAAGTATCTGATGATGCGATAATA
    GCCGGTAGCGGCGCTGCAATTATTGATGTCTCACGTGTTGCTCTTGATTACGCACTTACT
    GGCCTTGAATTTGCATGTGGTATTCCAGGTTCAATTGGTGGTGCAGTGTATATGAATGCT
    GGCGCTTATGGTGGCGAAGTTAAAGATTGTATAGACTATGCGCTTTGCGTAAACGAACAA
    GGCTCGTTAATTAAACTTACAACAAAAGAATTAGAGTTAGATTATCGTAATAGCATTATT
    CAAAAAGAACACTTAGTTGTATTAGAAGCTGCATTTACTTTAGCTCCTGGTAAAATGACT
    GAAATACAAGCTAAAATGGATGATTTAACAGAACGTAGAGAATCTAAACAACCTTTAGAG
    TATCCTTCATGTGGTAGTGTATTCCAAAGACCGCCTGGTCATTTTGCAGGTAAATTGATA
    CAAGATTCTAATTTGCAAGGTCACCGTATTGGCGGCGTTGAAGTTTCAACCAAACACGCT
    GGTTTTATGGTAAATGTAGACAATGGAACTGCTACAGATTATGAAAACCTTATTCATTAT
    GTACAAAAGACCGTCAAAGAAAAATTTGGCATTGAATTAAATCGTGAAGTTCGCATTATT
    GGTGAACATCCAAAGGAATCGTAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    924

Protein[edit source | edit]

General[edit source | edit]

  • locus tag: SACOL0801 [new locus tag: SACOL_RS04120 ]
  • symbol: MurB
  • description: UDP-N-acetylenolpyruvoylglucosamine reductase
  • length: 307
  • theoretical pI: 5.09771
  • theoretical MW: 33754.2
  • GRAVY: -0.214984

Function[edit source | edit]

  • reaction:
    EC 1.3.1.98?  ExPASy
    UDP-N-acetylmuramate dehydrogenaseUDP-N-acetyl-alpha-D-muramate + NADP+ = UDP-N-acetyl-3-O-(1-carboxyvinyl)-alpha-D-glucosamine + NADPH
    EC 1.1.1.158?  ExPASy
    Transferred entry: 1.3.1.98
  • TIGRFAM:
    Cell structureCell envelopeBiosynthesis and degradation of murein sacculus and peptidoglycanUDP-N-acetylenolpyruvoylglucosamine reductase (TIGR00179; EC 1.3.1.98; HMM-score: 240.2)
    Cellular processesCellular processesSporulation and germinationsporulation protein YtfJ (TIGR02874; HMM-score: 15)
    MetabolismEnergy metabolismOtherglycolate oxidase, subunit GlcD (TIGR00387; HMM-score: 14.5)
  • TheSEED:  
    Cell Wall and CapsuleCell Wall and Capsule - no subcategoryPeptidoglycan Biosynthesis UDP-N-acetylenolpyruvoylglucosamine reductase (EC 1.1.1.158) 
    Cell Wall and Capsule - no subcategoryUDP-N-acetylmuramate from Fructose-6-phosphate Biosynthesis UDP-N-acetylenolpyruvoylglucosamine reductase (EC 1.1.1.158) 
  • PFAM:
    no clan definedMurB_C; UDP-N-acetylenolpyruvoylglucosamine reductase, C-terminal domain (PF02873; HMM-score: 103.6)
    FAD_PCMH (CL0077) FAD_binding_4; FAD binding domain (PF01565; HMM-score: 90.1)
    no clan definedStomoxyn; Insect antimicrobial peptide, stomoxyn (PF11585; HMM-score: 13.2)

Structure, modifications & interactions[edit source | edit]

  • domains:
  • modifications:
  • cofactors: FAD
  • effectors:
  • protein partners:

Localization[edit source | edit]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 7.5
    • Cytoplasmic Membrane Score: 1.15
    • Cellwall Score: 0.62
    • Extracellular Score: 0.73
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular Possibility: 1
    • Signal Peptide Possibility: -1
    • N-terminally Anchored Score: -1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • Ymax: 0.123
    • Ymax_pos: 22
    • Cmax: 0.128
    • Cmax_pos: 52
    • Smax: 0.162
    • Smax_pos: 17
    • Smean: 0.119
    • D: 0.122
  • predicted transmembrane helices (TMHMM): 0

Accession numbers[edit source | edit]

Protein sequence[edit source | edit]

  • MINKDIYQALQQLIPNEKIKVDEPLKRYTYTKTGGNADFYITPTKNEEVQAVVKYAYQNEIPVTYLGNGSNIIIREGGIRGIVISLLSLDHIEVSDDAIIAGSGAAIIDVSRVALDYALTGLEFACGIPGSIGGAVYMNAGAYGGEVKDCIDYALCVNEQGSLIKLTTKELELDYRNSIIQKEHLVVLEAAFTLAPGKMTEIQAKMDDLTERRESKQPLEYPSCGSVFQRPPGHFAGKLIQDSNLQGHRIGGVEVSTKHAGFMVNVDNGTATDYENLIHYVQKTVKEKFGIELNREVRIIGEHPKES

Experimental data[edit source | edit]

  • experimentally validated: PeptideAtlas
    experimental localization: Cytoplasmic [1] [2] [3]
    quantitative data / protein copy number per cell: 144 [4]

Expression & Regulation[edit source | edit]

Operon[edit source | edit]

Regulation[edit source | edit]

  • sigma factor:
  • regulator:

Transcription pattern[edit source | edit]

Protein synthesis (provided by Aureolib)[edit source | edit]

Protein stability[edit source | edit]

  • half-life: 6.14 h [5]

Biological Material[edit source | edit]

Mutants[edit source | edit]

Expression vector[edit source | edit]

lacZ fusion[edit source | edit]

GFP fusion[edit source | edit]

two-hybrid system[edit source | edit]

FLAG-tag construct[edit source | edit]

Antibody[edit source | edit]

Other Information[edit source | edit]

You are kindly invited to share additional interesting facts.

Literature[edit source | edit]

References[edit source | edit]

  1. Dörte Becher, Kristina Hempel, Susanne Sievers, Daniela Zühlke, Jan Pané-Farré, Andreas Otto, Stephan Fuchs, Dirk Albrecht, Jörg Bernhardt, Susanne Engelmann, Uwe Völker, Jan Maarten van Dijl, Michael Hecker
    A proteomic view of an important human pathogen--towards the quantification of the entire Staphylococcus aureus proteome.
    PLoS ONE: 2009, 4(12);e8176
    [PubMed:19997597] [WorldCat.org] [DOI] (I e)
  2. Kristina Hempel, Florian-Alexander Herbst, Martin Moche, Michael Hecker, Dörte Becher
    Quantitative proteomic view on secreted, cell surface-associated, and cytoplasmic proteins of the methicillin-resistant human pathogen Staphylococcus aureus under iron-limited conditions.
    J. Proteome Res.: 2011, 10(4);1657-66
    [PubMed:21323324] [WorldCat.org] [DOI] (I p)
  3. Andreas Otto, Jan Maarten van Dijl, Michael Hecker, Dörte Becher
    The Staphylococcus aureus proteome.
    Int. J. Med. Microbiol.: 2014, 304(2);110-20
    [PubMed:24439828] [WorldCat.org] [DOI] (I p)
  4. Daniela Zühlke, Kirsten Dörries, Jörg Bernhardt, Sandra Maaß, Jan Muntel, Volkmar Liebscher, Jan Pané-Farré, Katharina Riedel, Michael Lalk, Uwe Völker, Susanne Engelmann, Dörte Becher, Stephan Fuchs, Michael Hecker
    Costs of life - Dynamics of the protein inventory of Staphylococcus aureus during anaerobiosis.
    Sci Rep: 2016, 6;28172
    [PubMed:27344979] [WorldCat.org] [DOI] (I e)
  5. Stephan Michalik, Jörg Bernhardt, Andreas Otto, Martin Moche, Dörte Becher, Hanna Meyer, Michael Lalk, Claudia Schurmann, Rabea Schlüter, Holger Kock, Ulf Gerth, Michael Hecker
    Life and death of proteins: a case study of glucose-starved Staphylococcus aureus.
    Mol. Cell Proteomics: 2012, 11(9);558-70
    [PubMed:22556279] [WorldCat.org] [DOI] (I p)

Relevant publications[edit source | edit]