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Pangenome04-0298108BA0217611819-9771193COLECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50N315NCTC8325NewmanRF122ST398T0131TCH60TW20USA300_FPR3757USA300_TCH1516VC40
NCBI: 10-JUN-2013

Summary[edit source | edit]

  • organism: Staphylococcus aureus COL
  • locus tag: SACOL1574 [new locus tag: SACOL_RS08020 ]
  • pan locus tag?: SAUPAN004090000
  • symbol: SACOL1574
  • pan gene symbol?:
  • synonym:
  • product: hypothetical protein

Genome View[edit source | edit]

Gene[edit source | edit]

General[edit source | edit]

  • type: CDS
  • locus tag: SACOL1574 [new locus tag: SACOL_RS08020 ]
  • symbol: SACOL1574
  • product: hypothetical protein
  • replicon: chromosome
  • strand: -
  • coordinates: 1609250..1609591
  • length: 342
  • essential: unknown

Accession numbers[edit source | edit]

Phenotype[edit source | edit]

  • Share your knowledge and add information here. [edit]

DNA sequence[edit source | edit]

  • 1
    61
    121
    181
    241
    301
    ATGAGGCGATGGTTTGTATTAATTTTAGGATTAGTTATATTATTATCTGCATGTGGTCAG
    AAATATGATAAAGAGATTGATGCAGTTTTAAATTCTGAAAGAAAAAGTATGAGTGAGTCT
    TCTTTTAAGAAGCCTGAAAAAAGTAATAGTGATTTTAAAGTATATGAAGATGGTAAATTT
    ATAACTATTTCTTTTGTTTATGATAAAGATGGTACAGTGTGGACAAGTTTATATAAAAAG
    AATGAGACAACAGATAAATATGTAAAAGTTGAAGATATGAATGAAAAAGAATATCAAAGT
    AATCATAAACCAGTTTATGAAGAAAATAATATGAAAAAATAA
    60
    120
    180
    240
    300
    342

Protein[edit source | edit]

General[edit source | edit]

  • locus tag: SACOL1574 [new locus tag: SACOL_RS08020 ]
  • symbol: SACOL1574
  • description: hypothetical protein
  • length: 113
  • theoretical pI: 8.38694
  • theoretical MW: 13355.1
  • GRAVY: -0.835398

Function[edit source | edit]

  • reaction:
  • TIGRFAM:
  • TheSEED:
  • PFAM:
    NTF2 (CL0051) DUF4467; Domain of unknown function with cystatin-like fold (DUF4467) (PF14729; HMM-score: 36.1)
    LppaM (CL0421) LPAM_2; Prokaryotic lipoprotein-attachment site (PF13627; HMM-score: 19.2)
    GPCR_A (CL0192) SID-1_RNA_chan; dsRNA-gated channel SID-1 (PF13965; HMM-score: 12.6)
    Beta_propeller (CL0186) Reg_prop; Two component regulator propeller (PF07494; HMM-score: 12.2)

Structure, modifications & interactions[edit source | edit]

  • domains:
  • modifications:
  • cofactors:
  • effectors:
  • protein partners:

Localization[edit source | edit]

  • PSORTb: unknown (no significant prediction)
    • Cytoplasmic Score: 2.5
    • Cytoplasmic Membrane Score: 2.5
    • Cellwall Score: 2.5
    • Extracellular Score: 2.5
    • Internal Helices: 0
  • LocateP: Lipid anchored
    • Prediction by SwissProt Classification: Extracellular
    • Pathway Prediction: Sec-(SPII)
    • Intracellular Possibility: 0
    • Signal Peptide Possibility: 0.5
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: LLSACGQ
  • SignalP: no predicted signal peptide
    • Ymax: 0.29
    • Ymax_pos: 23
    • Cmax: 0.166
    • Cmax_pos: 23
    • Smax: 0.656
    • Smax_pos: 3
    • Smean: 0.5
    • D: 0.372
  • predicted transmembrane helices (TMHMM): 0

Accession numbers[edit source | edit]

Protein sequence[edit source | edit]

  • MRRWFVLILGLVILLSACGQKYDKEIDAVLNSERKSMSESSFKKPEKSNSDFKVYEDGKFITISFVYDKDGTVWTSLYKKNETTDKYVKVEDMNEKEYQSNHKPVYEENNMKK

Experimental data[edit source | edit]

Expression & Regulation[edit source | edit]

Operon[edit source | edit]

Regulation[edit source | edit]

  • sigma factor:
  • regulator:

Transcription pattern[edit source | edit]

Protein synthesis (provided by Aureolib)[edit source | edit]

Protein stability[edit source | edit]

  • half-life: no data available

Biological Material[edit source | edit]

Mutants[edit source | edit]

Expression vector[edit source | edit]

lacZ fusion[edit source | edit]

GFP fusion[edit source | edit]

two-hybrid system[edit source | edit]

FLAG-tag construct[edit source | edit]

Antibody[edit source | edit]

Other Information[edit source | edit]

You are kindly invited to share additional interesting facts.

Literature[edit source | edit]

References[edit source | edit]

  1. Dörte Becher, Kristina Hempel, Susanne Sievers, Daniela Zühlke, Jan Pané-Farré, Andreas Otto, Stephan Fuchs, Dirk Albrecht, Jörg Bernhardt, Susanne Engelmann, Uwe Völker, Jan Maarten van Dijl, Michael Hecker
    A proteomic view of an important human pathogen--towards the quantification of the entire Staphylococcus aureus proteome.
    PLoS ONE: 2009, 4(12);e8176
    [PubMed:19997597] [WorldCat.org] [DOI] (I e)
  2. Kristina Hempel, Jan Pané-Farré, Andreas Otto, Susanne Sievers, Michael Hecker, Dörte Becher
    Quantitative cell surface proteome profiling for SigB-dependent protein expression in the human pathogen Staphylococcus aureus via biotinylation approach.
    J. Proteome Res.: 2010, 9(3);1579-90
    [PubMed:20108986] [WorldCat.org] [DOI] (I p)
  3. Kristina Hempel, Florian-Alexander Herbst, Martin Moche, Michael Hecker, Dörte Becher
    Quantitative proteomic view on secreted, cell surface-associated, and cytoplasmic proteins of the methicillin-resistant human pathogen Staphylococcus aureus under iron-limited conditions.
    J. Proteome Res.: 2011, 10(4);1657-66
    [PubMed:21323324] [WorldCat.org] [DOI] (I p)
  4. Andreas Otto, Jan Maarten van Dijl, Michael Hecker, Dörte Becher
    The Staphylococcus aureus proteome.
    Int. J. Med. Microbiol.: 2014, 304(2);110-20
    [PubMed:24439828] [WorldCat.org] [DOI] (I p)

Relevant publications[edit source | edit]