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NCBI: 02-MAR-2017

Summary[edit source | edit]

  • organism: Staphylococcus aureus COL
  • locus tag: SACOL_RS08540 [old locus tag: SACOL1674 ]
  • pan locus tag?: SAUPAN004217000
  • symbol: SACOL_RS08540
  • pan gene symbol?: recD2
  • synonym:
  • product: hypothetical protein

Genome View[edit source | edit]

Gene[edit source | edit]

General[edit source | edit]

  • type: CDS
  • locus tag: SACOL_RS08540 [old locus tag: SACOL1674 ]
  • symbol: SACOL_RS08540
  • product: hypothetical protein
  • replicon: chromosome
  • strand: -
  • coordinates: 1702192..1704669
  • length: 2478
  • essential: unknown

Accession numbers[edit source | edit]

  • Location: NC_002951 (1702192..1704669) NCBI
  • MicrobesOnline:

Phenotype[edit source | edit]

  • Share your knowledge and add information here. [edit]

DNA sequence[edit source | edit]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    ATGTCAGACCCTACACTATTTGATTATTCAATGATCAAAGGTACTGTTGAAGCGATATTG
    TTTCAAAACAGTGATAATTTTTATACAGTGCTTAAAGTAGATACTATTGAAACAAATGAA
    GACTTTGATACAATGCCAACTGTTGTAGGGTTTCTTCCGAATATTGTTGAAGGCGATGTC
    TACACATTTAAAGGGCAAGTTGTTGATCATCCACGTTATGGTAAACAATTAAAAGCAGAG
    ACTTTTGAAAAAGAAATGCCACAAACGAAAGAAGCAATTATAAGTTATTTATCTAGTGAT
    TTGTTTAAAGGTGTAGGTAAAAAGACAGCTCAAAATATTGTTAATACATTAGGTGACAAT
    GCGATAAATGATATATTAGATGACCATTCAGTGCTAGAAAAAGTTTCGGGATTATCTAAA
    AAGAAACAGAAACAAATTGCAGAACAAATTTCAGCAAATCAAGAATCTGAAAAAATAATG
    ATTCGTTTACATGATTTAGGGTTTGGTCCTAAACTTTCAATGGCAATTTATCAGTTTTAT
    CTCGGCGATACACTGACTATTTTAGATCGAAATCCATATCAATTGATTTATGATATTAAA
    GGTATTGGTTTTAATAAAGCAGATCAACTTGCTAGAAATATTGGTATCGCTTATAACGAT
    AATGAACGATTAAAAGCTGCGTTGCTCTATACGTTAGAAGAAGAATGTATTAAACAGGGA
    CATACATATTTGCCTATCAATGTTGTTATAGATTTAACGGTTGATGTATTAAATTATCAA
    GATGAAGAGGTCATCGAACCTGAAAAATTAGACGAAATGTTGCAATACTTAAATGAGGAA
    AAACGACTAATTATTGACAATGAACAAGTTGCAATTCCAAGTTTGTATTATTCTGAAATC
    AAAAGTGTTCAAAACTTATTCAGAATAAAGACGCATACAAATAAACTTACAGAAATTGAA
    CAGTCAGATTTACAAATGCATATTGGTGAAATAGAAGATGCGAATCAAGTGAACTATGCA
    GCTTCACAAAAAGAAGCTTTGCAAACGGCTATCAATTCTAAGGTGATGCTATTAACCGGT
    GGACCGGGAACAGGAAAAACGACAGTAATTAAAGGTATTGTTGAATTATATGCTGAGATT
    CATGGCTTATCATTAGATTATGATGATTATGTCAATGATGATTATCCAGTTGTATTAGCA
    GCGCCAACAGGAAGAGCGTCTAAGAGACTACAAGAGTCAACTGGATTAGAAGCAATGACG
    ATTCATCGTTTAATAGGTTGGAATCAAGACACAAAGCCAGAGGATATATTAGAAAATGAA
    ATAAACGCACGACTCATAATCATTGATGAGATGTCTATGGTAGATACTTGGTTGTTCCAC
    CAATTTTTAAGTGCAGTTCCTTTAGATGCACAATTGATATTTGTAGGTGATGAGGACCAA
    TTGCCTTCTGTAGGTCCTGGTCAAGTATTTAAAGATTTAATTGAATCGAAAGCTATACCA
    CGAGTAAATCTGACTGAAGTATATCGTCAACAAGATGGTTCAAGCATTATAGAATTAGCA
    CATCGAATGAAGTTGGGTCAGAAAATTGATATTACACAGCGTTTTCATGATAGAAGTTTT
    ATTAACTGTCAGGCAAACCAAATACCAACAGTTGTTGAGAAGGTAGTTACTAGTGCTGTT
    AATAAAGGGTATACTATGGCTGATATTCAAGTGCTTGCACCAATGTATAAAGGAAATGCC
    GGTATTAAACGTTTAAATCAAGTTTTACAAGATATTTTAAACCCTAAAAAGAAAGACACA
    CGAGAAATTGAGTTTGGTGATGTAGTCTTTAGAAAAGGTGACAAAGTATTACAACTTGTT
    AATAGGCCAAATGATAACATATTCAATGGTGACATAGGAGTTATTGTTGGTATTTTTTGG
    GCGAAAGAAAATGCCCTTAATAAAGATGTACTTGTTGTGGATTTTGAAGGTAATGAAATT
    ACATTTACGAAACAAGATATGATGGAATTAACACATGCTTATTGTACATCAATCCATAAA
    TCACAAGGTTCAGAATTTCCTATTGTTATCATGCCTATTGTTAAACAGTATTTTAGAATG
    TTACAAAGACCGATATTATATACTGGTTTAACTAGAGCGAAAACGAGTTTAGTTTTACTT
    GGTGATCCGGAAGCATTTGATATTGGATTGAAAACAAATGGTCAAGCGCGTTTAACACAA
    TTATGTACATTGTTGAAAAATTATTTTAATTCAGAAGATGAAGAAATGCTGGAAAACACA
    GCAACTAATGATACAGGAGCTTCACAAACGACAATAGATGATCAAGTAGAAGCGCCAATG
    TCATCGAATGATAGTGAAGAAGTTATGTCTGATTCAACAAAAACAGATACAAATGTATTA
    ACGGAAGCTACAATGTTTAAAATTGACCCAATGATTAATATGGGAGAAATCACGCCGTAT
    GACTTTATAGAACGTTGA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1200
    1260
    1320
    1380
    1440
    1500
    1560
    1620
    1680
    1740
    1800
    1860
    1920
    1980
    2040
    2100
    2160
    2220
    2280
    2340
    2400
    2460
    2478

Protein[edit source | edit]

General[edit source | edit]

  • locus tag: SACOL_RS08540 [old locus tag: SACOL1674 ]
  • symbol: SACOL_RS08540
  • description: hypothetical protein
  • length: 825
  • theoretical pI: 4.48109
  • theoretical MW: 93048.2
  • GRAVY: -0.29103

Function[edit source | edit]

  • reaction:
    EC 3.6.4.12?  ExPASy
    DNA helicaseATP + H2O = ADP + phosphate
  • TIGRFAM:
    Unknown functionEnzymes of unknown specificityhelicase, RecD/TraA family (TIGR01448; HMM-score: 1111.8)
    Genetic information processingDNA metabolismDNA replication, recombination, and repairexodeoxyribonuclease V, alpha subunit (TIGR01447; EC 3.1.11.5; HMM-score: 229.4)
    Ti-type conjugative transfer relaxase TraA (TIGR02768; HMM-score: 101.5)
    conjugative transfer relaxase protein TraI (TIGR02760; EC 3.6.4.12; HMM-score: 29.4)
    Genetic information processingDNA metabolismDNA replication, recombination, and repairHolliday junction DNA helicase RuvA (TIGR00084; EC 3.6.4.12; HMM-score: 28.6)
    Cellular processesCellular processesSporulation and germinationstage V sporulation protein K (TIGR02881; HMM-score: 25.2)
    Genetic information processingProtein fateProtein and peptide secretion and traffickingtype VII secretion AAA-ATPase EccA (TIGR03922; HMM-score: 21.1)
    P-type DNA transfer ATPase VirB11 (TIGR02788; HMM-score: 16.2)
    Cellular processesCellular processesConjugationP-type conjugative transfer ATPase TrbB (TIGR02782; HMM-score: 12.6)
    CbbX protein (TIGR02880; HMM-score: 12.3)
    Genetic information processingProtein synthesistRNA and rRNA base modificationtRNA threonylcarbamoyl adenosine modification protein YjeE (TIGR00150; HMM-score: 12.1)
    helicase/secretion neighborhood ATPase (TIGR03819; HMM-score: 11.2)
    Genetic information processingProtein fateProtein and peptide secretion and traffickingsignal recognition particle-docking protein FtsY (TIGR00064; HMM-score: 10.6)
  • TheSEED:
  • PFAM:
    P-loop_NTPase (CL0023) AAA_30; AAA domain (PF13604; HMM-score: 181.1)
    AAA_19; AAA domain (PF13245; HMM-score: 128)
    HHH (CL0198) HHH_4; Helix-hairpin-helix containing domain (PF14490; HMM-score: 108.2)
    P-loop_NTPase (CL0023) UvrD_C_2; UvrD-like helicase C-terminal domain (PF13538; HMM-score: 58.3)
    AAA_11; AAA domain (PF13086; HMM-score: 33.9)
    Viral_helicase1; Viral (Superfamily 1) RNA helicase (PF01443; HMM-score: 29.5)
    HHH (CL0198) HHH_5; Helix-hairpin-helix domain (PF14520; HMM-score: 23.3)
    P-loop_NTPase (CL0023) AAA; ATPase family associated with various cellular activities (AAA) (PF00004; HMM-score: 20.2)
    AAA_18; AAA domain (PF13238; HMM-score: 19.7)
    T2SSE; Type II/IV secretion system protein (PF00437; HMM-score: 19.4)
    AAA_12; AAA domain (PF13087; HMM-score: 19.2)
    AAA_14; AAA domain (PF13173; HMM-score: 16.4)
    AAA_29; P-loop containing region of AAA domain (PF13555; HMM-score: 14.8)
    NTPase_1; NTPase (PF03266; HMM-score: 14.7)
    TsaE; Threonylcarbamoyl adenosine biosynthesis protein TsaE (PF02367; HMM-score: 14.7)
    ABC_tran; ABC transporter (PF00005; HMM-score: 14.5)
    Mg_chelatase; Magnesium chelatase, subunit ChlI (PF01078; HMM-score: 14.3)
    5_3_exonuc_C (CL0464) 5_3_exonuc; 5'-3' exonuclease, C-terminal SAM fold (PF01367; HMM-score: 14.3)
    HHH (CL0198) HHH_3; Helix-hairpin-helix motif (PF12836; HMM-score: 14.1)
    P-loop_NTPase (CL0023) Helicase_RecD; Helicase (PF05127; HMM-score: 13.9)
    AAA_28; AAA domain (PF13521; HMM-score: 13.8)
    AAA_22; AAA domain (PF13401; HMM-score: 13.7)
    AAA_16; AAA ATPase domain (PF13191; HMM-score: 13.6)
    RsgA_GTPase; RsgA GTPase (PF03193; HMM-score: 13.4)
    UvrD_C; UvrD-like helicase C-terminal domain (PF13361; HMM-score: 13.2)
    Zeta_toxin; Zeta toxin (PF06414; HMM-score: 12.7)
    NACHT; NACHT domain (PF05729; HMM-score: 12.5)
    DUF815; Protein of unknown function (DUF815) (PF05673; HMM-score: 12.1)
    PhoH; PhoH-like protein (PF02562; HMM-score: 11.4)
    AAA_5; AAA domain (dynein-related subfamily) (PF07728; HMM-score: 9.7)
    AAA_17; AAA domain (PF13207; HMM-score: 9.4)

Structure, modifications & interactions[edit source | edit]

  • domains:
  • modifications:
  • cofactors:
  • effectors:
  • protein partners:

Localization[edit source | edit]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 7.5
    • Cytoplasmic Membrane Score: 1.15
    • Cellwall Score: 0.62
    • Extracellular Score: 0.73
    • Internal Helices: 0
  • LocateP:
  • SignalP: no predicted signal peptide
    • Ymax: 0.119
    • Ymax_pos: 34
    • Cmax: 0.128
    • Cmax_pos: 34
    • Smax: 0.163
    • Smax_pos: 26
    • Smean: 0.097
    • D: 0.11
  • predicted transmembrane helices (TMHMM): 0

Accession numbers[edit source | edit]

  • GI: 447206055 NCBI
  • RefSeq: WP_001283311 NCBI
  • UniProt:

Protein sequence[edit source | edit]

  • MSDPTLFDYSMIKGTVEAILFQNSDNFYTVLKVDTIETNEDFDTMPTVVGFLPNIVEGDVYTFKGQVVDHPRYGKQLKAETFEKEMPQTKEAIISYLSSDLFKGVGKKTAQNIVNTLGDNAINDILDDHSVLEKVSGLSKKKQKQIAEQISANQESEKIMIRLHDLGFGPKLSMAIYQFYLGDTLTILDRNPYQLIYDIKGIGFNKADQLARNIGIAYNDNERLKAALLYTLEEECIKQGHTYLPINVVIDLTVDVLNYQDEEVIEPEKLDEMLQYLNEEKRLIIDNEQVAIPSLYYSEIKSVQNLFRIKTHTNKLTEIEQSDLQMHIGEIEDANQVNYAASQKEALQTAINSKVMLLTGGPGTGKTTVIKGIVELYAEIHGLSLDYDDYVNDDYPVVLAAPTGRASKRLQESTGLEAMTIHRLIGWNQDTKPEDILENEINARLIIIDEMSMVDTWLFHQFLSAVPLDAQLIFVGDEDQLPSVGPGQVFKDLIESKAIPRVNLTEVYRQQDGSSIIELAHRMKLGQKIDITQRFHDRSFINCQANQIPTVVEKVVTSAVNKGYTMADIQVLAPMYKGNAGIKRLNQVLQDILNPKKKDTREIEFGDVVFRKGDKVLQLVNRPNDNIFNGDIGVIVGIFWAKENALNKDVLVVDFEGNEITFTKQDMMELTHAYCTSIHKSQGSEFPIVIMPIVKQYFRMLQRPILYTGLTRAKTSLVLLGDPEAFDIGLKTNGQARLTQLCTLLKNYFNSEDEEMLENTATNDTGASQTTIDDQVEAPMSSNDSEEVMSDSTKTDTNVLTEATMFKIDPMINMGEITPYDFIER

Experimental data[edit source | edit]

  • experimentally validated: no data available

Expression & Regulation[edit source | edit]

Operon[edit source | edit]

  • SACOL_RS08540 no polycistronic organisation predicted

Regulation[edit source | edit]

  • sigma factor:
  • regulator:

Transcription pattern[edit source | edit]

Protein synthesis (provided by Aureolib)[edit source | edit]

Protein stability[edit source | edit]

  • half-life: no data available

Biological Material[edit source | edit]

Mutants[edit source | edit]

Expression vector[edit source | edit]

lacZ fusion[edit source | edit]

GFP fusion[edit source | edit]

two-hybrid system[edit source | edit]

FLAG-tag construct[edit source | edit]

Antibody[edit source | edit]

Other Information[edit source | edit]

You are kindly invited to share additional interesting facts.

Literature[edit source | edit]

References[edit source | edit]

Relevant publications[edit source | edit]