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NCBI: 03-AUG-2016

Summary[edit source | edit]

  • organism: Staphylococcus aureus NCTC8325
  • locus tag: SAOUHSC_00025
  • pan locus tag?: SAUPAN000037000
  • symbol: SAOUHSC_00025
  • pan gene symbol?: adsA
  • synonym:
  • product: hypothetical protein

Genome View[edit source | edit]

Gene[edit source | edit]

General[edit source | edit]

  • type: CDS
  • locus tag: SAOUHSC_00025
  • symbol: SAOUHSC_00025
  • product: hypothetical protein
  • replicon: chromosome
  • strand: +
  • coordinates: 31005..33323
  • length: 2319
  • essential: no DEG other strains

Accession numbers[edit source | edit]

Phenotype[edit source | edit]

  • Share your knowledge and add information here. [edit]

DNA sequence[edit source | edit]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    ATGAAAGCTTTATTACTTAAAACAAGTGTATGGCTCGTTTTGCTTTTTAGTGTAATGGGA
    TTATGGCAAGTCTCGAACGCGGCTGAGCAGCATACACCAATGAAAGCACATGCAGTAACA
    ACGATAGACAAAGCAACAACAGATAAGCAACAAGTACCGCCAACAAAGGAAGCGGCTCAT
    CATTCTGGCAAAGAAGCGGCAACCAACGTATCAGCATCAGCGCAGGGAACAGCTGATGAT
    ACAAACAGCAAAGTAACATCCAACGCACCATCTAACAAACCATCTACAGTAGTTTCAACA
    AAAGTAAACGAAACACGCGACGTAGATACACAACAAGCCTCAACACAAAAACCAACTCAC
    ACAGCAACGTTCAAATTATCAAATGCTAAAACAGCATCACTTTCACCACGAATGTTTGCT
    GCTAATGCACCACAAACAACAACACATAAAATATTACATACAAATGATATCCATGGCCGA
    CTAGCCGAAGAAAAAGGGCGTGTCATCGGTATGGCTAAATTAAAAACAGTAAAAGAACAA
    GAAAAGCCTGATTTAATGTTAGACGCAGGAGACGCCTTCCAAGGTTTACCACTTTCAAAC
    CAGTCTAAAGGTGAAGAAATGGCTAAAGCAATGAATGCAGTAGGTTATGATGCTATGGCA
    GTCGGTAACCATGAATTTGACTTTGGATACGATCAGTTGAAAAAGTTAGAGGGTATGTTA
    GACTTCCCGATGCTAAGTACTAACGTTTATAAAGATGGAAAACGCGCGTTTAAGCCTTCA
    ACGATTGTAACAAAAAATGGTATTCGTTATGGAATTATTGGTGTAACGACACCAGAAACA
    AAGACGAAAACAAGACCTGAAGGCATTAAAGGCGTTGAATTTAGAGATCCATTACAAAGT
    GTGACAGCGGAAATGATGCGTATTTATAAAGACGTAGATACATTTGTTGTTATATCACAT
    TTAGGAATTGATCCTTCAACACAAGAAACATGGCGTGGTGATTACTTAGTGAAACAATTA
    AGTCAAAATCCACAATTGAAGAAACGTATTACAGTTATTGATGGTCATTCACATACAGTA
    CTTCAAAATGGTCAAATTTATAACAATGATGCATTGGCACAAACAGGTACAGCACTTGCG
    AATATCGGTAAGATTACATTTAATTATCGCAATGGAGAGGTATCGAATATTAAACCGTCA
    TTGATTAATGTTAAAGACGTTGAAAATGTAACACCGAACAAAGCATTAGCTGAACAAATT
    AATCAAGCTGATCAAACATTTAGAGCACAAACTGCAGAGGTAATTATTCCAAACAATACC
    ATTGATTTCAAAGGAGAAAGAGATGACGTTAGAACGCGTGAAACAAATTTAGGAAACGCG
    ATTGCAGATGCTATGGAAGCGTATGGCGTTAAGAATTTCTCTAAAAAGACTGACTTTGCC
    GTGACAAATGGTGGAGGTATTCGTGCCTCTATCGCAAAAGGTAAGGTGACACGCTATGAT
    TTAATCTCAGTATTACCATTTGGAAATACGATTGCGCAAATTGATGTAAAAGGTTCAGAC
    GTCTGGACGGCTTTCGAACATAGTTTAGGCGCACCAACAACACAAAAGGACGGTAAGACA
    GTGTTAACAGCGAATGGCGGTTTACTACATATCTCTGATTCAATCCGTGTTTACTATGAT
    ATAAATAAACCGTCTGGCAAACGAATTAATGCTATTCAAATTTTAAATAAAGAGACAGGT
    AAGTTTGAAAATATTGATTTAAAACGTGTATATCACGTAACGATGAATGACTTCACAGCA
    TCAGGTGGCGACGGATATAGTATGTTCGGTGGTCCTAGAGAAGAAGGTATTTCATTAGAT
    CAAGTACTAGCAAGTTATTTAAAAACAGCTAACTTAGCTAAGTATGATACGACAGAACCA
    CAACGTATGTTATTAGGTAAACCAGCAGTAAGTGAACAACCAGCTAAAGGACAACAAGGT
    AGCAAAGGTAGTAAGTCTGGTAAAGATACACAACCAATTGGTGACGACAAAGTGATGGAT
    CCAGCGAAAAAACCAGCTCCAGGTAAAGTTGTATTGTTGCTAGCGCATAGAGGAACTGTT
    AGTAGCGGTACAGAAGGTTCTGGTCGCACAATAGAAGGAGCTACTGTATCAAGCAAGAGT
    GGGAAACAATTGGCTAGAATGTCAGTGCCTAAAGGTAGCGCGCATGAGAAACAGTTACCA
    AAAACTGGAACTAATCAAAGTTCAAGCCCAGAAGCGATGTTTGTATTATTAGCAGGTATA
    GGTTTAATCGCGACTGTACGACGTAGAAAAGCTAGCTAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1200
    1260
    1320
    1380
    1440
    1500
    1560
    1620
    1680
    1740
    1800
    1860
    1920
    1980
    2040
    2100
    2160
    2220
    2280
    2319

Protein[edit source | edit]

General[edit source | edit]

  • locus tag: SAOUHSC_00025
  • symbol: SAOUHSC_00025
  • description: hypothetical protein
  • length: 772
  • theoretical pI: 10.0493
  • theoretical MW: 83424
  • GRAVY: -0.480959

Function[edit source | edit]

  • reaction:
  • TIGRFAM:
    MetabolismBiosynthesis of cofactors, prosthetic groups, and carriersPyridine nucleotidesNAD nucleotidase (TIGR01530; EC 3.1.3.5,3.6.1.22; HMM-score: 134.4)
    MetabolismTransport and binding proteinsOtherNAD nucleotidase (TIGR01530; EC 3.1.3.5,3.6.1.22; HMM-score: 134.4)
    thiosulfohydrolase SoxB (TIGR04486; HMM-score: 91.9)
    MetabolismPurines, pyrimidines, nucleosides, and nucleotidesOther2',3'-cyclic-nucleotide 2'-phosphodiesterase (TIGR01390; EC 3.1.4.16; HMM-score: 52.7)
    Cell structureCell envelopeOtherLPXTG cell wall anchor domain (TIGR01167; HMM-score: 22.2)
    Genetic information processingDNA metabolismDNA replication, recombination, and repairexonuclease SbcCD, D subunit (TIGR00619; HMM-score: 13.1)
    aspartate-alanine antiporter (TIGR03802; HMM-score: 10.4)
    Genetic information processingProtein fateProtein and peptide secretion and traffickingmembrane protein insertase, YidC/Oxa1 family, N-terminal domain (TIGR03593; HMM-score: 8)
  • TheSEED:  
    Nucleosides and NucleotidesPurinesPurine conversions 5'-nucleotidase (EC 3.1.3.5) 
    Pyrimidinespyrimidine conversions 5'-nucleotidase (EC 3.1.3.5) 
    Virulence, Disease and DefenseAdhesionAdhesins in Staphylococcus Virulence-associated cell-wall-anchored protein SasH (LPXTG motif) 
  • PFAM:
    no clan defined5_nucleotid_C; 5'-nucleotidase, C-terminal domain (PF02872; HMM-score: 141.4)
    Calcineurin (CL0163) Metallophos; Calcineurin-like phosphoesterase (PF00149; HMM-score: 43.6)
    no clan definedIES5; Ino80 complex subunit 5 (PF17335; HMM-score: 14.2)
    Gram_pos_anchor; LPXTG cell wall anchor motif (PF00746; HMM-score: 9.6)

Structure, modifications & interactions[edit source | edit]

  • domains:
  • modifications:
  • cofactors:
  • effectors:
  • protein partners:

Localization[edit source | edit]

  • PSORTb: Cellwall
    • Cytoplasmic Score: 0
    • Cytoplasmic Membrane Score: 0
    • Cellwall Score: 10
    • Extracellular Score: 0
    • Internal Helix: 1
  • LocateP: LPxTG Cell-wall anchored
    • Prediction by SwissProt Classification: Cell Wall
    • Pathway Prediction: Sec-(SPI)
    • Intracellular Possibility: 0.17
    • Signal Peptide Possibility: 1
    • N-terminally Anchored Score: -1
    • Predicted Cleavage Site: found LPxTG motif :LPKTG
  • SignalP: Signal peptide length 28 aa
    • Ymax: 0.761
    • Ymax_pos: 28
    • Cmax: 0.691
    • Cmax_pos: 28
    • Smax: 0.911
    • Smax_pos: 20
    • Smean: 0.848
    • D: 0.795
  • predicted transmembrane helices (TMHMM): 0

Accession numbers[edit source | edit]

Protein sequence[edit source | edit]

  • MKALLLKTSVWLVLLFSVMGLWQVSNAAEQHTPMKAHAVTTIDKATTDKQQVPPTKEAAHHSGKEAATNVSASAQGTADDTNSKVTSNAPSNKPSTVVSTKVNETRDVDTQQASTQKPTHTATFKLSNAKTASLSPRMFAANAPQTTTHKILHTNDIHGRLAEEKGRVIGMAKLKTVKEQEKPDLMLDAGDAFQGLPLSNQSKGEEMAKAMNAVGYDAMAVGNHEFDFGYDQLKKLEGMLDFPMLSTNVYKDGKRAFKPSTIVTKNGIRYGIIGVTTPETKTKTRPEGIKGVEFRDPLQSVTAEMMRIYKDVDTFVVISHLGIDPSTQETWRGDYLVKQLSQNPQLKKRITVIDGHSHTVLQNGQIYNNDALAQTGTALANIGKITFNYRNGEVSNIKPSLINVKDVENVTPNKALAEQINQADQTFRAQTAEVIIPNNTIDFKGERDDVRTRETNLGNAIADAMEAYGVKNFSKKTDFAVTNGGGIRASIAKGKVTRYDLISVLPFGNTIAQIDVKGSDVWTAFEHSLGAPTTQKDGKTVLTANGGLLHISDSIRVYYDINKPSGKRINAIQILNKETGKFENIDLKRVYHVTMNDFTASGGDGYSMFGGPREEGISLDQVLASYLKTANLAKYDTTEPQRMLLGKPAVSEQPAKGQQGSKGSKSGKDTQPIGDDKVMDPAKKPAPGKVVLLLAHRGTVSSGTEGSGRTIEGATVSSKSGKQLARMSVPKGSAHEKQLPKTGTNQSSSPEAMFVLLAGIGLIATVRRRKAS

Experimental data[edit source | edit]

Expression & Regulation[edit source | edit]

Operon[edit source | edit]

Regulation[edit source | edit]

  • sigma factor:
  • regulator:

Transcription pattern[edit source | edit]

Protein synthesis (provided by Aureolib)[edit source | edit]

Protein stability[edit source | edit]

  • half-life: no data available

Biological Material[edit source | edit]

Mutants[edit source | edit]

Expression vector[edit source | edit]

lacZ fusion[edit source | edit]

GFP fusion[edit source | edit]

two-hybrid system[edit source | edit]

FLAG-tag construct[edit source | edit]

Antibody[edit source | edit]

Other Information[edit source | edit]

You are kindly invited to share additional interesting facts.

Literature[edit source | edit]

References[edit source | edit]

  1. Maren Depke, Stephan Michalik, Alexander Rabe, Kristin Surmann, Lars Brinkmann, Nico Jehmlich, Jörg Bernhardt, Michael Hecker, Bernd Wollscheid, Zhi Sun, Robert L Moritz, Uwe Völker, Frank Schmidt
    A peptide resource for the analysis of Staphylococcus aureus in host-pathogen interaction studies.
    Proteomics: 2015, 15(21);3648-3661
    [PubMed:26224020] [WorldCat.org] [DOI] (I p)
  2. Ulrike Mäder, Pierre Nicolas, Maren Depke, Jan Pané-Farré, Michel Debarbouille, Magdalena M van der Kooi-Pol, Cyprien Guérin, Sandra Dérozier, Aurelia Hiron, Hanne Jarmer, Aurélie Leduc, Stephan Michalik, Ewoud Reilman, Marc Schaffer, Frank Schmidt, Philippe Bessières, Philippe Noirot, Michael Hecker, Tarek Msadek, Uwe Völker, Jan Maarten van Dijl
    Staphylococcus aureus Transcriptome Architecture: From Laboratory to Infection-Mimicking Conditions.
    PLoS Genet.: 2016, 12(4);e1005962
    [PubMed:27035918] [WorldCat.org] [DOI] (I e)

Relevant publications[edit source | edit]

Vilasack Thammavongsa, Justin W Kern, Dominique M Missiakas, Olaf Schneewind
Staphylococcus aureus synthesizes adenosine to escape host immune responses.
J. Exp. Med.: 2009, 206(11);2417-27
[PubMed:19808256] [WorldCat.org] [DOI] (I p)
Vilasack Thammavongsa, Olaf Schneewind, Dominique M Missiakas
Enzymatic properties of Staphylococcus aureus adenosine synthase (AdsA).
BMC Biochem.: 2011, 12;56
[PubMed:22035583] [WorldCat.org] [DOI] (I e)
Hwan Keun Kim, Vilasack Thammavongsa, Olaf Schneewind, Dominique Missiakas
Recurrent infections and immune evasion strategies of Staphylococcus aureus.
Curr. Opin. Microbiol.: 2012, 15(1);92-9
[PubMed:22088393] [WorldCat.org] [DOI] (I p)