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04-0298108BA0217611819-9771193COLECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50N315NCTC8325NewmanRF122ST398T0131TCH60TW20USA300_FPR3757USA300_TCH1516VC40
NCBI: 03-AUG-2016

Summary[edit source | edit]

  • organism: Staphylococcus aureus NCTC8325
  • locus tag: SAOUHSC_01515
  • pan locus tag?:
  • symbol: SAOUHSC_01515
  • pan gene symbol?:
  • synonym:
  • product: petidoglycan hydrolase

Genome View[edit source | edit]

Gene[edit source | edit]

General[edit source | edit]

  • type: CDS
  • locus tag: SAOUHSC_01515
  • symbol: SAOUHSC_01515
  • product: petidoglycan hydrolase
  • replicon: chromosome
  • strand: -
  • coordinates: 1464415..1465869
  • length: 1455
  • essential: unknown

Accession numbers[edit source | edit]

Phenotype[edit source | edit]

  • Share your knowledge and add information here. [edit]

DNA sequence[edit source | edit]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    ATGTTGATAACAAAAAACCAAGCAGAAAAATGGTTTGATAATTCATTAGGGAAGCAGTTC
    AATCCTGATTTGTTTTATGGATTTCAGTGTTACGATTACGCAAATATGTTTTTTATGATA
    GCAACAGGCGAAAGGTTACAAGGTTTATACGCTTATAATATTCCATTTGATAATAAAGCA
    AGGATTGAAAAATACGGGCAAATAATTAAAAACTATGATAGCTTTTTACCGCAAAAGTTG
    GACATTGTCGTTTTCCCGTCAAAGTATGGTGGCGGAGCTGGACATGTTGAAATTGTTGAG
    AGCGCTAATCTAAACACTTTCACATCGTTTGGCCAAAATTGGAATGGTAAAGGTTGGACA
    AATGGCGTTGCGCAACCTGGTTGGGGTCCCGAAACCGTTACAAGACATGTTCATTATTAC
    GATGACCCAATGTATTTTATTAGATTAAATTTCCCAGATAAAGTAAGTGTTGGAGATAAA
    GCTAAAAGCGTTATTAAGCAAGCAACTGCCAAAAAGCAAGCAGTAATTAAACCTAAAAAA
    ATTATGCTTGTAGCCGGTCATGGTTATAACGATCCTGGAGCAGTCGGAAACGGAACAAAT
    GAACGTGATTTTATCCGTAAATATATAACACCAAATATCGCTAAGTATTTAAGACATGCA
    GGTCACGAAGTTGCATTATATGGTGGCTCAAGTCAATCACAAGATATGTATCAAGATACT
    GCTTACGGTGTTAATGTAGGAAATAATAAAGATTATGGCTTATATTGGGTTAAATCACAT
    GGGTATGACATTGTTCTAGAGATTCATTTAGACGCAGCAGGAGAAAGTGCAAGTGGTGGG
    CATGTTATTATTTCAAGTCAATTCAATGCAGATACTATTGATAAAAGTATACAAGATGTT
    ATTAAAAATAACTTAGGACAAATAAGAGGTGTAACACCTCGTAATGATTTACTAAACGTT
    AATGTATCAGCAGAAATAAATATAAACTATCGTTTATCTGAATTAGGTTTTATTACTAAT
    AAAAATGATATGGATTGGATTAAGAAAAACTATGACTTGTATTCTAAATTAATAGCCGGT
    GCGATTCATGGTAAGCCTATAGGTGGTTTGGTAGCTGGTAATGTTAAAACATCAGCTAAA
    AACCAAAAAAATCCACCAGTGCCAGCAGGTTATACACTTGATAAAAACAATGTACCGTAT
    AAAAAAGAGACTGGTTATTACACAGTTGCCAATGTTAAAGGTAATAACGTAAGGGACGGC
    TATTCAACTAATTCAAGAATTACAGGTGTATTACCTAATAACGCAACAATTAAATATGAC
    GGCGCATATTGCATCAATGGCTATAGATGGATTACTTATATTGCTAATAGTGGACAACGT
    CGTTATATAGCGACAGGAGAGGTAGACAAGGCAGGTAATAGAATAAGTAGTTTTGGTAAG
    TTTAGCACGATTTAG
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1200
    1260
    1320
    1380
    1440
    1455

Protein[edit source | edit]

General[edit source | edit]

  • locus tag: SAOUHSC_01515
  • symbol: SAOUHSC_01515
  • description: petidoglycan hydrolase
  • length: 484
  • theoretical pI: 9.59909
  • theoretical MW: 53829.2
  • GRAVY: -0.472934

Function[edit source | edit]

  • reaction:
  • TIGRFAM:
    Cell structureCell envelopeBiosynthesis and degradation of murein sacculus and peptidoglycanN-acetylmuramoyl-L-alanine amidase CwlD (TIGR02883; EC 3.5.1.28; HMM-score: 43.4)
    Cellular processesCellular processesSporulation and germinationN-acetylmuramoyl-L-alanine amidase CwlD (TIGR02883; EC 3.5.1.28; HMM-score: 43.4)
  • TheSEED:  
    Phage lysin, N-acetylmuramoyl-L-alanine amidase (EC 3.5.1.28) 
  • PFAM:
    Peptidase_MH (CL0035) Amidase_3; N-acetylmuramoyl-L-alanine amidase (PF01520; HMM-score: 137.1)
    Peptidase_CA (CL0125) CHAP; CHAP domain (PF05257; HMM-score: 36.4)
    SH3 (CL0010) SH3_5; Bacterial SH3 domain (PF08460; HMM-score: 31.8)
    SH3_3; Bacterial SH3 domain (PF08239; HMM-score: 16)

Structure, modifications & interactions[edit source | edit]

  • domains:
  • modifications:
  • cofactors:
  • effectors:
  • protein partners:

Localization[edit source | edit]

  • PSORTb: unknown (no significant prediction)
    • Cytoplasmic Score: 2.5
    • Cytoplasmic Membrane Score: 2.5
    • Cellwall Score: 2.5
    • Extracellular Score: 2.5
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular Possibility: 1
    • Signal Peptide Possibility: -1
    • N-terminally Anchored Score: -1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • Ymax: 0.115
    • Ymax_pos: 11
    • Cmax: 0.113
    • Cmax_pos: 52
    • Smax: 0.224
    • Smax_pos: 6
    • Smean: 0.134
    • D: 0.122
  • predicted transmembrane helices (TMHMM): 0

Accession numbers[edit source | edit]

Protein sequence[edit source | edit]

  • MLITKNQAEKWFDNSLGKQFNPDLFYGFQCYDYANMFFMIATGERLQGLYAYNIPFDNKARIEKYGQIIKNYDSFLPQKLDIVVFPSKYGGGAGHVEIVESANLNTFTSFGQNWNGKGWTNGVAQPGWGPETVTRHVHYYDDPMYFIRLNFPDKVSVGDKAKSVIKQATAKKQAVIKPKKIMLVAGHGYNDPGAVGNGTNERDFIRKYITPNIAKYLRHAGHEVALYGGSSQSQDMYQDTAYGVNVGNNKDYGLYWVKSHGYDIVLEIHLDAAGESASGGHVIISSQFNADTIDKSIQDVIKNNLGQIRGVTPRNDLLNVNVSAEININYRLSELGFITNKNDMDWIKKNYDLYSKLIAGAIHGKPIGGLVAGNVKTSAKNQKNPPVPAGYTLDKNNVPYKKETGYYTVANVKGNNVRDGYSTNSRITGVLPNNATIKYDGAYCINGYRWITYIANSGQRRYIATGEVDKAGNRISSFGKFSTI

Experimental data[edit source | edit]

  • experimentally validated: no data available

Expression & Regulation[edit source | edit]

Operon[edit source | edit]

Regulation[edit source | edit]

  • sigma factor:
  • regulator:

Transcription pattern[edit source | edit]

Protein synthesis (provided by Aureolib)[edit source | edit]

Protein stability[edit source | edit]

  • half-life: no data available

Biological Material[edit source | edit]

Mutants[edit source | edit]

Expression vector[edit source | edit]

lacZ fusion[edit source | edit]

GFP fusion[edit source | edit]

two-hybrid system[edit source | edit]

FLAG-tag construct[edit source | edit]

Antibody[edit source | edit]

Other Information[edit source | edit]

You are kindly invited to share additional interesting facts.

Literature[edit source | edit]

References[edit source | edit]

  1. Ulrike Mäder, Pierre Nicolas, Maren Depke, Jan Pané-Farré, Michel Debarbouille, Magdalena M van der Kooi-Pol, Cyprien Guérin, Sandra Dérozier, Aurelia Hiron, Hanne Jarmer, Aurélie Leduc, Stephan Michalik, Ewoud Reilman, Marc Schaffer, Frank Schmidt, Philippe Bessières, Philippe Noirot, Michael Hecker, Tarek Msadek, Uwe Völker, Jan Maarten van Dijl
    Staphylococcus aureus Transcriptome Architecture: From Laboratory to Infection-Mimicking Conditions.
    PLoS Genet.: 2016, 12(4);e1005962
    [PubMed:27035918] [WorldCat.org] [DOI] (I e)

Relevant publications[edit source | edit]