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NCBI: 03-AUG-2016

Summary[edit source | edit]

  • organism: Staphylococcus aureus NCTC8325
  • locus tag: SAOUHSC_01833
  • pan locus tag?: SAUPAN004379000
  • symbol: SAOUHSC_01833
  • pan gene symbol?: serA
  • synonym:
  • product: D-3-phosphoglycerate dehydrogenase

Genome View[edit source | edit]

Gene[edit source | edit]

General[edit source | edit]

  • type: CDS
  • locus tag: SAOUHSC_01833
  • symbol: SAOUHSC_01833
  • product: D-3-phosphoglycerate dehydrogenase
  • replicon: chromosome
  • strand: +
  • coordinates: 1737925..1739529
  • length: 1605
  • essential: no DEG other strains

Accession numbers[edit source | edit]

Phenotype[edit source | edit]

  • Share your knowledge and add information here. [edit]

DNA sequence[edit source | edit]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    ATGAAGCAATTTAATGTACTCGTTGCAGATCCCATATCAAAAGATGGTATCAAAGCATTA
    TTAGATCACGAACAATTCAATGTAGATATTCAAACTGGCTTGTCCGAAGAAGCATTAATC
    AAAATTATACCTTCATACCATGCTTTAATCGTTCGTAGTCAAACTACGGTTACTGAAAAT
    ATCATAAATGCTGCTGATTCTTTAAAAGTAATCGCACGCGCCGGTGTTGGTGTAGATAAT
    ATTAATATTGATGCTGCAACATTAAAAGGTATTTTAGTTATTAATGCCCCAGATGGTAAT
    ACGATTTCAGCTACTGAACATACACTGGCAATGTTATTATCAATGGCACGAAATATTCCG
    CAAGCACACCAATCACTTACAAATAAAGAATGGAATCGAAATGCATTTAAAGGTACTGAG
    CTTTATCATAAAACATTAGGTGTCATTGGTGCTGGTAGAATTGGTTTAGGTGTTGCTAAA
    CGTGCGCAAAGTTTCGGAATGAAAATACTAGCTTTTGACCCTTACTTAACGGATGAAAAA
    GCAAAATCTTTAAGCATTACGAAGGCAACAGTTGATGAGATTGCCCAACATTCTGATTTC
    GTTACATTACATACACCACTAACACCTAAAACAAAAGGCTTAATTAATGCTGTCTTTTTT
    GCCAAAGCAAAACCTAGTTTGCAAATAATCAATGTGGCACGTGGTGGTATTATTGATGAA
    AAGGCGCTAATAAAAGCATTAGACGAAGGACAAATTAGTCGGGCAGCTATCGATGTGTTT
    GAACATGAACCTGCAACTGACTCGCCTCTTGTTGCACATGATAAAATTATTGTTACACCT
    CATTTGGGTGCTTCAACAGTCGAAGCTCAAGAAAAAGTGGCAATTTCTGTTTCAAATGAA
    ATCATCGAAATTTTAATTGATGGTACTGTAACGCATGCAGTGAATGCACCTAAAATGGAC
    TTAAGCAATATAGATGATACTGTAAAATCATTCATCAATTTAAGCCAAACAGTTGGTGAA
    TTAGCTATTCAATTAATGTACAATGCACCAAGCTCTATTAAAATTACGTACGGTGGCGAC
    TTAGCCTCTATTGATAGTAGTTTATTAACACGTACAATTATTACTCATATTTTAAAAGAT
    GATCTTGGTCCTGAAGTCAATATTATCAATGCTCTAATGTTGTTAAATCAACAACAAGTG
    ACATTAAATATTGAAAATAATAAAGCAGAGACAGGTTTTAGTAACTACTTAGAGGTAGAA
    CTATCAAACGATAGCGATTCCGTTAAAGTTGGCGCTTCTGTCTTTACAGGTTTCGGTCCA
    AGAATTGTTAGAATTAATAATTTTTCTGTAGACTTAAAGCCAAATCAATATCAAATTGTG
    TCATATCATAATGATACTCCAGGTATGGTAGGAAAAACTGGCGCATTGTTAGGTAAATAC
    AATATCAACATTGCATCTATGACTTTAGGTAGAACTGAAGCGGGCGGAGATGCGCTAATG
    ATTTTATCCGTTGATCAACCTGTTTCAAACAATATAATTGATGAACTTAAACAAGTTGGT
    GAATACAATCAAATTTTCACAACTGAATTGACGGTACAGTCATAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1200
    1260
    1320
    1380
    1440
    1500
    1560
    1605

Protein[edit source | edit]

General[edit source | edit]

  • locus tag: SAOUHSC_01833
  • symbol: SAOUHSC_01833
  • description: D-3-phosphoglycerate dehydrogenase
  • length: 534
  • theoretical pI: 5.30951
  • theoretical MW: 57618.4
  • GRAVY: 0.0337079

Function[edit source | edit]

  • reaction:
    EC 1.1.1.95?  ExPASy
    Phosphoglycerate dehydrogenase3-phospho-D-glycerate + NAD+ = 3-phosphonooxypyruvate + NADH2-hydroxyglutarate + NAD+ = 2-oxoglutarate + NADH
  • TIGRFAM:
    MetabolismAmino acid biosynthesisSerine familyphosphoglycerate dehydrogenase (TIGR01327; EC 1.1.1.95; HMM-score: 745.7)
    MetabolismEnergy metabolismAmino acids and aminesL-serine dehydratase, iron-sulfur-dependent, beta subunit (TIGR00719; EC 4.3.1.17; HMM-score: 38.8)
    MetabolismEnergy metabolismGlycolysis/gluconeogenesisL-serine dehydratase, iron-sulfur-dependent, beta subunit (TIGR00719; EC 4.3.1.17; HMM-score: 38.8)
  • TheSEED:  
    Amino Acids and DerivativesAlanine, serine, and glycineGlycine and Serine Utilization D-3-phosphoglycerate dehydrogenase (EC 1.1.1.95) 
    Alanine, serine, and glycineSerine Biosynthesis D-3-phosphoglycerate dehydrogenase (EC 1.1.1.95) 
    Cofactors, Vitamins, Prosthetic Groups, PigmentsPyridoxinePyridoxin (Vitamin B6) Biosynthesis D-3-phosphoglycerate dehydrogenase (EC 1.1.1.95) 
  • PFAM:
    NADP_Rossmann (CL0063) 2-Hacid_dh_C; D-isomer specific 2-hydroxyacid dehydrogenase, NAD binding domain (PF02826; HMM-score: 209.9)
    Form_Glyc_dh (CL0325) 2-Hacid_dh; D-isomer specific 2-hydroxyacid dehydrogenase, catalytic domain (PF00389; HMM-score: 119.2)
    ACT (CL0070) ACT; ACT domain (PF01842; HMM-score: 38.6)
    NADP_Rossmann (CL0063) NAD_binding_2; NAD binding domain of 6-phosphogluconate dehydrogenase (PF03446; HMM-score: 18.7)
    F420_oxidored; NADP oxidoreductase coenzyme F420-dependent (PF03807; HMM-score: 16.9)
    3HCDH_N; 3-hydroxyacyl-CoA dehydrogenase, NAD binding domain (PF02737; HMM-score: 14.1)

Structure, modifications & interactions[edit source | edit]

  • domains:
  • modifications:
  • cofactors:
  • effectors:
  • protein partners:

Localization[edit source | edit]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 9.67
    • Cytoplasmic Membrane Score: 0.01
    • Cellwall Score: 0.15
    • Extracellular Score: 0.17
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular Possibility: 1
    • Signal Peptide Possibility: -1
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • Ymax: 0.15
    • Ymax_pos: 65
    • Cmax: 0.23
    • Cmax_pos: 65
    • Smax: 0.174
    • Smax_pos: 19
    • Smean: 0.092
    • D: 0.128
  • predicted transmembrane helices (TMHMM): 0

Accession numbers[edit source | edit]

Protein sequence[edit source | edit]

  • MKQFNVLVADPISKDGIKALLDHEQFNVDIQTGLSEEALIKIIPSYHALIVRSQTTVTENIINAADSLKVIARAGVGVDNINIDAATLKGILVINAPDGNTISATEHTLAMLLSMARNIPQAHQSLTNKEWNRNAFKGTELYHKTLGVIGAGRIGLGVAKRAQSFGMKILAFDPYLTDEKAKSLSITKATVDEIAQHSDFVTLHTPLTPKTKGLINAVFFAKAKPSLQIINVARGGIIDEKALIKALDEGQISRAAIDVFEHEPATDSPLVAHDKIIVTPHLGASTVEAQEKVAISVSNEIIEILIDGTVTHAVNAPKMDLSNIDDTVKSFINLSQTVGELAIQLMYNAPSSIKITYGGDLASIDSSLLTRTIITHILKDDLGPEVNIINALMLLNQQQVTLNIENNKAETGFSNYLEVELSNDSDSVKVGASVFTGFGPRIVRINNFSVDLKPNQYQIVSYHNDTPGMVGKTGALLGKYNINIASMTLGRTEAGGDALMILSVDQPVSNNIIDELKQVGEYNQIFTTELTVQS

Experimental data[edit source | edit]

Expression & Regulation[edit source | edit]

Operon[edit source | edit]

Regulation[edit source | edit]

  • sigma factor:
  • regulator: SAOUHSC_01228 (CodY*)
    CodY* (TF) important in Amino acid metabolism  RegPrecise    transcription unit transferred from N315 data RegPrecise

Transcription pattern[edit source | edit]

Protein synthesis (provided by Aureolib)[edit source | edit]

Protein stability[edit source | edit]

  • half-life: no data available

Biological Material[edit source | edit]

Mutants[edit source | edit]

Expression vector[edit source | edit]

lacZ fusion[edit source | edit]

GFP fusion[edit source | edit]

two-hybrid system[edit source | edit]

FLAG-tag construct[edit source | edit]

Antibody[edit source | edit]

Other Information[edit source | edit]

You are kindly invited to share additional interesting facts.

Literature[edit source | edit]

References[edit source | edit]

  1. Stephan Michalik, Maren Depke, Annette Murr, Manuela Gesell Salazar, Ulrike Kusebauch, Zhi Sun, Tanja C Meyer, Kristin Surmann, Henrike Pförtner, Petra Hildebrandt, Stefan Weiss, Laura Marcela Palma Medina, Melanie Gutjahr, Elke Hammer, Dörte Becher, Thomas Pribyl, Sven Hammerschmidt, Eric W Deutsch, Samuel L Bader, Michael Hecker, Robert L Moritz, Ulrike Mäder, Uwe Völker, Frank Schmidt
    A global Staphylococcus aureus proteome resource applied to the in vivo characterization of host-pathogen interactions.
    Sci Rep: 2017, 7(1);9718
    [PubMed:28887440] [WorldCat.org] [DOI] (I e)
  2. Maren Depke, Stephan Michalik, Alexander Rabe, Kristin Surmann, Lars Brinkmann, Nico Jehmlich, Jörg Bernhardt, Michael Hecker, Bernd Wollscheid, Zhi Sun, Robert L Moritz, Uwe Völker, Frank Schmidt
    A peptide resource for the analysis of Staphylococcus aureus in host-pathogen interaction studies.
    Proteomics: 2015, 15(21);3648-61
    [PubMed:26224020] [WorldCat.org] [DOI] (I p)
  3. Ulrike Mäder, Pierre Nicolas, Maren Depke, Jan Pané-Farré, Michel Debarbouille, Magdalena M van der Kooi-Pol, Cyprien Guérin, Sandra Dérozier, Aurelia Hiron, Hanne Jarmer, Aurélie Leduc, Stephan Michalik, Ewoud Reilman, Marc Schaffer, Frank Schmidt, Philippe Bessières, Philippe Noirot, Michael Hecker, Tarek Msadek, Uwe Völker, Jan Maarten van Dijl
    Staphylococcus aureus Transcriptome Architecture: From Laboratory to Infection-Mimicking Conditions.
    PLoS Genet.: 2016, 12(4);e1005962
    [PubMed:27035918] [WorldCat.org] [DOI] (I e)

Relevant publications[edit source | edit]