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NCBI: 03-AUG-2016

Summary[edit source | edit]

  • organism: Staphylococcus aureus NCTC8325
  • locus tag: SAOUHSC_02363
  • pan locus tag?: SAUPAN005414000
  • symbol: SAOUHSC_02363
  • pan gene symbol?:
  • synonym:
  • product: aldehyde dehydrogenase

Genome View[edit source | edit]

Gene[edit source | edit]

General[edit source | edit]

  • type: CDS
  • locus tag: SAOUHSC_02363
  • symbol: SAOUHSC_02363
  • product: aldehyde dehydrogenase
  • replicon: chromosome
  • strand: -
  • coordinates: 2184537..2185964
  • length: 1428
  • essential: no DEG other strains

Accession numbers[edit source | edit]

Phenotype[edit source | edit]

Share your knowledge and add information here. [edit]

DNA sequence[edit source | edit]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    ATGAGAGACTACACAAAGCAATACATTAATGGCGAATGGGTAGAAAGTAATAGTAATGAA
    ACGATAGAAGTTATAAATCCAGCAACCGAAGAAGTAATCGGGAAAGTTGCTAAAGGTAAT
    AAAGCTGATGTTGATAAAGCCGTCGAGGCGGCAGACGATGTTTATTTAGAGTTCCGTCAT
    ACATCTGTGAAAGAAAGACAAGCGTTATTAGATAAAATTGTAAAAGAATATGAAAACAGA
    AAAGACGATATTGTACAAGCTATTACGGATGAATTAGGTGCTCCTTTATCATTATCTGAG
    CGTGTCCATTATCAAATGGGACTAAACCATTTTGTTGCAGCGAGAGACGCATTAGATAAC
    TACGAATTTGAAGAACGCCGCGGAGATGATTTAGTTGTTAAAGAAGCAATCGGTGTATCT
    GGATTAATTACACCGTGGAACTTCCCTACAAACCAAACATCATTAAAATTAGCAGCAGCA
    TTTGCGGCTGGTAGTCCAGTTGTACTTAAACCATCTGAAGAAACACCATTTGCAGCTGTT
    ATTTTAGCTGAGATTTTTGATAAAGTCGGTGTTCCTAAAGGTGTATTTAACCTTGTTAAT
    GGTGATGGTGCTGGTGTTGGGAATCCTTTATCTGAACATCCTAAAGTACGCATGATGTCA
    TTTACAGGATCAGGCCCTACTGGTTCTAAAATTATGGAAAAAGCCGCTAAAGATTTTAAA
    AAGGTATCATTAGAGCTTGGTGGCAAATCACCATATATCGTCCTAGATGACGTAGATATT
    AAAGAAGCGGCTAAAGCAACAACAGGCAAAGTTGTTAATAATACTGGTCAAGTATGTACA
    GCTGGTACACGTGTTTTAGTGCCTAACAAAATTAAAGATGCATTCTTAGCTGAATTAAAA
    GAACAATTTAGCCAAGTGCGTGTCGGTAATCCAAGAGAAGATGGTACACAAGTAGGCCCT
    ATCATTAGTAAAAAACAATTTGATCAAGTACAAAATTATATTAATAAAGGTATTGAAGAA
    GGTGCTGAATTATTTTATGGTGGTCCTGGTAAACCAGAAGGACTTGAAAAAGGATACTTT
    GCACGTCCGACAATTTTTATTAATGTAGATAATCAAATGACGATAGCACAAGAAGAAATT
    TTTGGGCCAGTAATGTCAGTTATCACTTATAACGATTTAGATGAAGCGATTCAAATTGCA
    AATGATACAAAATATGGTTTGGCAGGATATGTTATTGGTAAGGACAAAGAAACATTGCAT
    AAAGTAGCTCGTTCTATTGAAGCAGGTACAGTAGAAATAAACGAAGCAGGTAGAAAGCCA
    GATTTACCATTTGGTGGCTATAAACAATCTGGTTTAGGTCGTGAATGGGGCGATTATGGT
    ATTGAAGAGTTCTTAGAAGTGAAATCTATAGCTGGATATTTTAAATAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1200
    1260
    1320
    1380
    1428

Protein[edit source | edit]

General[edit source | edit]

  • locus tag: SAOUHSC_02363
  • symbol: SAOUHSC_02363
  • description: aldehyde dehydrogenase
  • length: 475
  • theoretical pI: 4.76962
  • theoretical MW: 51968.3
  • GRAVY: -0.350526

Function[edit source | edit]

  • reaction:
    EC 1.2.1.3?  ExPASy
    Aldehyde dehydrogenase (NAD+)An aldehyde + NAD+ + H2O = a carboxylate + NADH
    EC 1.2.1.16?  ExPASy
    Succinate-semialdehyde dehydrogenase (NAD(P)+)Succinate semialdehyde + NAD(P)+ + H2O = succinate + NAD(P)H
  • TIGRFAM:
    Cellular processesCellular processesAdaptations to atypical conditionsbetaine-aldehyde dehydrogenase (TIGR01804; EC 1.2.1.8; HMM-score: 460)
    5-carboxymethyl-2-hydroxymuconate semialdehyde dehydrogenase (TIGR02299; EC 1.2.1.60; HMM-score: 449.2)
    Unknown functionEnzymes of unknown specificityaldehyde dehydrogenase, Rv0768 family (TIGR04284; EC 1.2.1.-; HMM-score: 405)
    MetabolismCentral intermediary metabolismOthersuccinate-semialdehyde dehydrogenase (TIGR01780; EC 1.2.1.-; HMM-score: 403.8)
    MetabolismEnergy metabolismOther2-hydroxymuconic semialdehyde dehydrogenase (TIGR03216; EC 1.2.1.-; HMM-score: 375.8)
    and 10 more
    MetabolismEnergy metabolismAmino acids and aminesputative delta-1-pyrroline-5-carboxylate dehydrogenase (TIGR01237; EC 1.2.1.88; HMM-score: 314.6)
    1-pyrroline dehydrogenase (TIGR03374; EC 1.2.1.19; HMM-score: 293.2)
    MetabolismEnergy metabolismAmino acids and aminesmethylmalonate-semialdehyde dehydrogenase (acylating) (TIGR01722; EC 1.2.1.27; HMM-score: 274.6)
    putative phosphonoacetaldehyde dehydrogenase (TIGR03250; EC 1.2.1.-; HMM-score: 265.1)
    MetabolismEnergy metabolismAmino acids and aminessuccinylglutamate-semialdehyde dehydrogenase (TIGR03240; EC 1.2.1.71; HMM-score: 223.8)
    MetabolismEnergy metabolismAmino acids and aminesdelta-1-pyrroline-5-carboxylate dehydrogenase (TIGR01238; EC 1.2.1.88; HMM-score: 181.5)
    MetabolismEnergy metabolismAmino acids and amines1-pyrroline-5-carboxylate dehydrogenase (TIGR01236; EC 1.2.1.88; HMM-score: 169.2)
    phenylacetic acid degradation protein paaN (TIGR02278; HMM-score: 142.6)
    phenylacetic acid degradation protein paaN (TIGR02288; HMM-score: 48.8)
    acetaldehyde dehydrogenase (acetylating) (TIGR02518; EC 1.2.1.10; HMM-score: 23.1)
  • TheSEED:  
    CarbohydratesCentral carbohydrate metabolismMethylglyoxal Metabolism Aldehyde dehydrogenase (EC 1.2.1.3) 
    and 2 more
    Central carbohydrate metabolismPyruvate metabolism II: acetyl-CoA, acetogenesis from pyruvate Aldehyde dehydrogenase (EC 1.2.1.3) 
    Fatty Acids, Lipids, and IsoprenoidsPhospholipidsGlycerolipid and Glycerophospholipid Metabolism in Bacteria Aldehyde dehydrogenase (EC 1.2.1.3) 
  • PFAM:
    ALDH-like (CL0099) Aldedh; Aldehyde dehydrogenase family (PF00171; HMM-score: 552.9)

Structure, modifications & interactions[edit source | edit]

  • domains:
  • modifications:
  • cofactors:
  • effectors:
  • protein partners:
    SAOUHSC_00529elongation factor G  [1] (data from MRSA252)
    SAOUHSC_00530elongation factor Tu  [1] (data from MRSA252)
    SAOUHSC_01806pyruvate kinase  [1] (data from MRSA252)

Localization[edit source | edit]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 9.97
    • Cytoplasmic Membrane Score: 0
    • Cellwall Score: 0.01
    • Extracellular Score: 0.02
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular Possibility: 1
    • Signal Peptide Possibility: -1
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • Ymax: 0.103
    • Ymax_pos: 43
    • Cmax: 0.135
    • Cmax_pos: 43
    • Smax: 0.106
    • Smax_pos: 30
    • Smean: 0.075
    • D: 0.092
  • predicted transmembrane helices (TMHMM): 0

Accession numbers[edit source | edit]

Protein sequence[edit source | edit]

  • MRDYTKQYINGEWVESNSNETIEVINPATEEVIGKVAKGNKADVDKAVEAADDVYLEFRHTSVKERQALLDKIVKEYENRKDDIVQAITDELGAPLSLSERVHYQMGLNHFVAARDALDNYEFEERRGDDLVVKEAIGVSGLITPWNFPTNQTSLKLAAAFAAGSPVVLKPSEETPFAAVILAEIFDKVGVPKGVFNLVNGDGAGVGNPLSEHPKVRMMSFTGSGPTGSKIMEKAAKDFKKVSLELGGKSPYIVLDDVDIKEAAKATTGKVVNNTGQVCTAGTRVLVPNKIKDAFLAELKEQFSQVRVGNPREDGTQVGPIISKKQFDQVQNYINKGIEEGAELFYGGPGKPEGLEKGYFARPTIFINVDNQMTIAQEEIFGPVMSVITYNDLDEAIQIANDTKYGLAGYVIGKDKETLHKVARSIEAGTVEINEAGRKPDLPFGGYKQSGLGREWGDYGIEEFLEVKSIAGYFK

Experimental data[edit source | edit]

Expression & Regulation[edit source | edit]

Operon[edit source | edit]

Regulation[edit source | edit]

  • sigma factor: SAOUHSC_02298 regulon (SigB*) [4] [5] other strains
    SigB*(sigma factor)controlling a large regulon involved in stress/starvation response and adaptation
  • regulator:

Transcription pattern[edit source | edit]

Protein synthesis (provided by Aureolib)[edit source | edit]

Protein stability[edit source | edit]

  • half-life: no data available

Biological Material[edit source | edit]

Mutants[edit source | edit]

Expression vector[edit source | edit]

lacZ fusion[edit source | edit]

GFP fusion[edit source | edit]

two-hybrid system[edit source | edit]

FLAG-tag construct[edit source | edit]

Antibody[edit source | edit]

Other Information[edit source | edit]

You are kindly invited to share additional interesting facts.

Literature[edit source | edit]

References[edit source | edit]

  1. 1.0 1.1 1.2 Artem Cherkasov, Michael Hsing, Roya Zoraghi, Leonard J Foster, Raymond H See, Nikolay Stoynov, Jihong Jiang, Sukhbir Kaur, Tian Lian, Linda Jackson, Huansheng Gong, Rick Swayze, Emily Amandoron, Farhad Hormozdiari, Phuong Dao, Cenk Sahinalp, Osvaldo Santos-Filho, Peter Axerio-Cilies, Kendall Byler, William R McMaster, Robert C Brunham, B Brett Finlay, Neil E Reiner
    Mapping the protein interaction network in methicillin-resistant Staphylococcus aureus.
    J. Proteome Res.: 2011, 10(3);1139-50
    [PubMed:21166474] [WorldCat.org] [DOI] (I p)
  2. Stephan Michalik, Maren Depke, Annette Murr, Manuela Gesell Salazar, Ulrike Kusebauch, Zhi Sun, Tanja C Meyer, Kristin Surmann, Henrike Pförtner, Petra Hildebrandt, Stefan Weiss, Laura Marcela Palma Medina, Melanie Gutjahr, Elke Hammer, Dörte Becher, Thomas Pribyl, Sven Hammerschmidt, Eric W Deutsch, Samuel L Bader, Michael Hecker, Robert L Moritz, Ulrike Mäder, Uwe Völker, Frank Schmidt
    A global Staphylococcus aureus proteome resource applied to the in vivo characterization of host-pathogen interactions.
    Sci Rep: 2017, 7(1);9718
    [PubMed:28887440] [WorldCat.org] [DOI] (I e)
  3. Maren Depke, Stephan Michalik, Alexander Rabe, Kristin Surmann, Lars Brinkmann, Nico Jehmlich, Jörg Bernhardt, Michael Hecker, Bernd Wollscheid, Zhi Sun, Robert L Moritz, Uwe Völker, Frank Schmidt
    A peptide resource for the analysis of Staphylococcus aureus in host-pathogen interaction studies.
    Proteomics: 2015, 15(21);3648-61
    [PubMed:26224020] [WorldCat.org] [DOI] (I p)
  4. Markus Bischoff, Paul Dunman, Jan Kormanec, Daphne Macapagal, Ellen Murphy, William Mounts, Brigitte Berger-Bächi, Steven Projan
    Microarray-based analysis of the Staphylococcus aureus sigmaB regulon.
    J. Bacteriol.: 2004, 186(13);4085-99
    [PubMed:15205410] [WorldCat.org] [DOI] (P p)
  5. 5.0 5.1 Ulrike Mäder, Pierre Nicolas, Maren Depke, Jan Pané-Farré, Michel Debarbouille, Magdalena M van der Kooi-Pol, Cyprien Guérin, Sandra Dérozier, Aurelia Hiron, Hanne Jarmer, Aurélie Leduc, Stephan Michalik, Ewoud Reilman, Marc Schaffer, Frank Schmidt, Philippe Bessières, Philippe Noirot, Michael Hecker, Tarek Msadek, Uwe Völker, Jan Maarten van Dijl
    Staphylococcus aureus Transcriptome Architecture: From Laboratory to Infection-Mimicking Conditions.
    PLoS Genet.: 2016, 12(4);e1005962
    [PubMed:27035918] [WorldCat.org] [DOI] (I e)

Relevant publications[edit source | edit]