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NCBI: 03-AUG-2016

Summary[edit source | edit]

  • organism: Staphylococcus aureus NCTC8325
  • locus tag: SAOUHSC_02365
  • pan locus tag?: SAUPAN005416000
  • symbol: SAOUHSC_02365
  • pan gene symbol?: murA2
  • synonym:
  • product: UDP-N-acetylglucosamine 1-carboxyvinyltransferase

Genome View[edit source | edit]

Gene[edit source | edit]

General[edit source | edit]

  • type: CDS
  • locus tag: SAOUHSC_02365
  • symbol: SAOUHSC_02365
  • product: UDP-N-acetylglucosamine 1-carboxyvinyltransferase
  • replicon: chromosome
  • strand: -
  • coordinates: 2186626..2187885
  • length: 1260
  • essential: no DEG other strains

Accession numbers[edit source | edit]

Phenotype[edit source | edit]

  • Share your knowledge and add information here. [edit]

DNA sequence[edit source | edit]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    ATGGCTCAAGAGGTAATAAAAATAAGAGGTGGACGCACACTAAATGGGGAAGTTAATATC
    AGTGGCGCGAAAAACAGTGCTGTAGCTATCATTCCTGCAACATTATTAGCTCAAGGACAT
    GTGAAATTAGAAGGGTTACCGCAAATCTCTGATGTTAAAACTTTAGTGAGTTTATTAGAA
    GATTTAAATATTAAGGCATCACTAAATGGTACGGAATTAGAAGTTGATACAACTGAAATA
    CAAAATGCTGCATTACCAAATAATAAAGTTGAATCATTACGTGCTTCATACTATATGATG
    GGTGCCATGTTAGGTAGATTTAAAAAATGTGTGATTGGTTTACCGGGAGGATGTCCACTT
    GGGCCACGTCCAATTGATCAACATATTAAAGGATTTAAAGCTTTAGGTGCTGAAATTGAT
    GAATCAAGTACGACATCAATGAAAATCGAAGCTAAAGAGTTAAAAGGTGCACATATTTTT
    CTAGATATGGTTAGCGTAGGTGCAACAATTAACATTATGCTAGCTGCAGTTTATGCAACA
    GGACAAACTGTAATTGAAAATGCTGCTAAAGAACCGGAAGTAGTTGATGTTGCTAACTTC
    TTAACAAGTATGGGAGCTAATATTAAGGGAGCGGGTACATCAACAATTAAAATCAATGGC
    GTCAAAGAATTACATGGTTCTGAGTATCAAGTTATACCTGATAGAATTGAAGCAGGCACA
    TATATGTGTATCGCTGCAGCATGTGGAGAAAATGTTATATTAAATAATATTGTTCCGAAA
    CATGTAGAAACATTAACTGCTAAATTTAGTGAATTAGGTGTGAATGTTGATGTAAGAGAC
    GAAAGAATTCGCATCAATAATAATGCGCCATATCAATTTGTTGATATTAAAACACTAGTA
    TATCCAGGTTTTGCTACTGATTTGCAACAGCCTATTACACCATTATTATTTATGGCAAAT
    GGTCCTTCATTTGTAACTGATACGATTTATCCGGAGCGTTTTAAACATGTTGAAGAATTA
    AAGCGCATGGGTGCAAATATTGAAGTTGACGAAGGCACAGCAACGATAAAACCATCAACA
    TTACATGGTGCTGAAGTTTATGCTAGCGATTTAAGAGCAGGAGCTTGTTTAATTATTGCT
    GGTTTAATTGCTGAGGGTGTAACTACAATTTACAATGTTAAACATATTTATAGAGGTTAT
    ACAGATATTGTAGAACACTTAAAAGCTTTAGGTGCAGATATTTGGACGGAAACTGTATAG
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1200
    1260

Protein[edit source | edit]

General[edit source | edit]

  • locus tag: SAOUHSC_02365
  • symbol: SAOUHSC_02365
  • description: UDP-N-acetylglucosamine 1-carboxyvinyltransferase
  • length: 419
  • theoretical pI: 5.46141
  • theoretical MW: 45074.7
  • GRAVY: 0.072315

Function[edit source | edit]

  • reaction:
    EC 2.5.1.7?  ExPASy
    UDP-N-acetylglucosamine 1-carboxyvinyltransferasePhosphoenolpyruvate + UDP-N-acetyl-alpha-D-glucosamine = phosphate + UDP-N-acetyl-3-O-(1-carboxyvinyl)-alpha-D-glucosamine
  • TIGRFAM:
    Cell structureCell envelopeBiosynthesis and degradation of murein sacculus and peptidoglycanUDP-N-acetylglucosamine 1-carboxyvinyltransferase (TIGR01072; EC 2.5.1.7; HMM-score: 526.9)
    MetabolismAmino acid biosynthesisAromatic amino acid family3-phosphoshikimate 1-carboxyvinyltransferase (TIGR01356; EC 2.5.1.19; HMM-score: 87.8)
  • TheSEED:  
    Cell Wall and CapsuleCell Wall and Capsule - no subcategoryPeptidoglycan Biosynthesis UDP-N-acetylglucosamine 1-carboxyvinyltransferase (EC 2.5.1.7) 
    Cell Wall and Capsule - no subcategoryUDP-N-acetylmuramate from Fructose-6-phosphate Biosynthesis UDP-N-acetylglucosamine 1-carboxyvinyltransferase (EC 2.5.1.7) 
  • PFAM:
    EPT_RTPC (CL0290) EPSP_synthase; EPSP synthase (3-phosphoshikimate 1-carboxyvinyltransferase) (PF00275; HMM-score: 300.5)

Structure, modifications & interactions[edit source | edit]

Localization[edit source | edit]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 7.5
    • Cytoplasmic Membrane Score: 1.15
    • Cellwall Score: 0.62
    • Extracellular Score: 0.73
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular Possibility: 1
    • Signal Peptide Possibility: -1
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • Ymax: 0.173
    • Ymax_pos: 30
    • Cmax: 0.161
    • Cmax_pos: 30
    • Smax: 0.364
    • Smax_pos: 36
    • Smean: 0.138
    • D: 0.16
  • predicted transmembrane helices (TMHMM): 0

Accession numbers[edit source | edit]

Protein sequence[edit source | edit]

  • MAQEVIKIRGGRTLNGEVNISGAKNSAVAIIPATLLAQGHVKLEGLPQISDVKTLVSLLEDLNIKASLNGTELEVDTTEIQNAALPNNKVESLRASYYMMGAMLGRFKKCVIGLPGGCPLGPRPIDQHIKGFKALGAEIDESSTTSMKIEAKELKGAHIFLDMVSVGATINIMLAAVYATGQTVIENAAKEPEVVDVANFLTSMGANIKGAGTSTIKINGVKELHGSEYQVIPDRIEAGTYMCIAAACGENVILNNIVPKHVETLTAKFSELGVNVDVRDERIRINNNAPYQFVDIKTLVYPGFATDLQQPITPLLFMANGPSFVTDTIYPERFKHVEELKRMGANIEVDEGTATIKPSTLHGAEVYASDLRAGACLIIAGLIAEGVTTIYNVKHIYRGYTDIVEHLKALGADIWTETV

Experimental data[edit source | edit]

Expression & Regulation[edit source | edit]

Operon[edit source | edit]

Regulation[edit source | edit]

  • sigma factor:
  • regulator:

Transcription pattern[edit source | edit]

Protein synthesis (provided by Aureolib)[edit source | edit]

Protein stability[edit source | edit]

  • half-life: no data available

Biological Material[edit source | edit]

Mutants[edit source | edit]

Expression vector[edit source | edit]

lacZ fusion[edit source | edit]

GFP fusion[edit source | edit]

two-hybrid system[edit source | edit]

FLAG-tag construct[edit source | edit]

Antibody[edit source | edit]

Other Information[edit source | edit]

You are kindly invited to share additional interesting facts.

Literature[edit source | edit]

References[edit source | edit]

  1. 1.0 1.1 1.2 1.3 1.4 1.5 1.6 Artem Cherkasov, Michael Hsing, Roya Zoraghi, Leonard J Foster, Raymond H See, Nikolay Stoynov, Jihong Jiang, Sukhbir Kaur, Tian Lian, Linda Jackson, Huansheng Gong, Rick Swayze, Emily Amandoron, Farhad Hormozdiari, Phuong Dao, Cenk Sahinalp, Osvaldo Santos-Filho, Peter Axerio-Cilies, Kendall Byler, William R McMaster, Robert C Brunham, B Brett Finlay, Neil E Reiner
    Mapping the protein interaction network in methicillin-resistant Staphylococcus aureus.
    J. Proteome Res.: 2011, 10(3);1139-50
    [PubMed:21166474] [WorldCat.org] [DOI] (I p)
  2. Stephan Michalik, Maren Depke, Annette Murr, Manuela Gesell Salazar, Ulrike Kusebauch, Zhi Sun, Tanja C Meyer, Kristin Surmann, Henrike Pförtner, Petra Hildebrandt, Stefan Weiss, Laura Marcela Palma Medina, Melanie Gutjahr, Elke Hammer, Dörte Becher, Thomas Pribyl, Sven Hammerschmidt, Eric W Deutsch, Samuel L Bader, Michael Hecker, Robert L Moritz, Ulrike Mäder, Uwe Völker, Frank Schmidt
    A global Staphylococcus aureus proteome resource applied to the in vivo characterization of host-pathogen interactions.
    Sci Rep: 2017, 7(1);9718
    [PubMed:28887440] [WorldCat.org] [DOI] (I e)
  3. Maren Depke, Stephan Michalik, Alexander Rabe, Kristin Surmann, Lars Brinkmann, Nico Jehmlich, Jörg Bernhardt, Michael Hecker, Bernd Wollscheid, Zhi Sun, Robert L Moritz, Uwe Völker, Frank Schmidt
    A peptide resource for the analysis of Staphylococcus aureus in host-pathogen interaction studies.
    Proteomics: 2015, 15(21);3648-3661
    [PubMed:26224020] [WorldCat.org] [DOI] (I p)
  4. Ulrike Mäder, Pierre Nicolas, Maren Depke, Jan Pané-Farré, Michel Debarbouille, Magdalena M van der Kooi-Pol, Cyprien Guérin, Sandra Dérozier, Aurelia Hiron, Hanne Jarmer, Aurélie Leduc, Stephan Michalik, Ewoud Reilman, Marc Schaffer, Frank Schmidt, Philippe Bessières, Philippe Noirot, Michael Hecker, Tarek Msadek, Uwe Völker, Jan Maarten van Dijl
    Staphylococcus aureus Transcriptome Architecture: From Laboratory to Infection-Mimicking Conditions.
    PLoS Genet.: 2016, 12(4);e1005962
    [PubMed:27035918] [WorldCat.org] [DOI] (I e)

Relevant publications[edit source | edit]