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Pangenome04-0298108BA0217611819-9771193COLECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50N315NCTC8325NewmanRF122ST398T0131TCH60TW20USA300_FPR3757USA300_TCH1516VC40
NCBI: 03-AUG-2016

Summary[edit source | edit]

  • organism: Staphylococcus aureus NCTC8325
  • locus tag: SAOUHSC_02798
  • pan locus tag?: SAUPAN006108000
  • symbol: SAOUHSC_02798
  • pan gene symbol?: sasG
  • synonym:
  • product: hypothetical protein

Genome View[edit source | edit]

Gene[edit source | edit]

General[edit source | edit]

  • type: CDS
  • locus tag: SAOUHSC_02798
  • symbol: SAOUHSC_02798
  • product: hypothetical protein
  • replicon: chromosome
  • strand: -
  • coordinates: 2570009..2574892
  • length: 4884
  • essential: no DEG

Accession numbers[edit source | edit]

Phenotype[edit source | edit]

  • Share your knowledge and add information here. [edit]

DNA sequence[edit source | edit]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    ATGAGAGATAAGAAAGGACCGGTAAATAAAAGAGTAGATTTTCTATCAAATAAATTGAAT
    AAATATTCAATAAGAAAATTTACAGTTGGAACAGCATCTATTTTAATTGGCTCACTAATG
    TATTTGGGAACTCAACAAGAGGCAGAAGCAGCTGAAAACAATATTGAGAATCCAACTACA
    TTAAAAGATAATGTCCAATCAAAAGAAGTGAAGATTGAAGAAGTAACAAACAAAGACACT
    GCACCACAGGGTGTAGAAGCTAAATCTGAAGTAACTTCAAACAAAGACACAATCGAACAT
    GAACCATCAGTAAAAGCTGAAGATATATCAAAAAAGGAGGATACACCAAAAGAAGTAGCT
    GATGTTGCTGAAGTTCAGCCGAAATCGTCAGTCACTCATAACGCAGAGACACCTAAGGTT
    AGAAAAGCTCGTTCTGTTGATGAAGGCTCTTTTGATATTACAAGAGATTCTAAAAATGTA
    GTTGAATCTACCCCAATTACAATTCAAGGTAAAGAACATTTTGAAGGTTACGGAAGTGTT
    GATATACAAAAAAAACCAACAGATTTAGGGGTATCAGAGGTAACCAGGTTTAATGTTGGT
    AATGAAAGTAATGGTTTGATAGGAGCTTTACAATTAAAAAATAAAATAGATTTTAGTAAG
    GATTTCAATTTTAAAGTTAGAGTGGCAAATAACCATCAATCAAATACCACAGGTGCTGAT
    GGTTGGGGGTTCTTATTTAGTAAAGGAAATGCAGAAGAATATTTAACTAATGGTGGAATC
    CTTGGGGATAAAGGTCTGGTAAATTCAGGCGGATTTAAAATTGATACTGGATACATTTAT
    ACAAGTTCCATGGACAAAACTGAAAAGCAAGCTGGACAAGGTTATAGAGGATACGGAGCT
    TTTGTGAAAAATGACAGTTCTGGTAATTCACAAATGGTTGGAGAAAATATTGATAAATCA
    AAAACTAATTTTTTAAACTATGCGGACAATTCAACTAATACATCAGATGGAAAGTTTCAT
    GGGCAACGTTTAAATGATGTCATCTTAACTTATGTTGCTTCAACTGGTAAAATGAGAGCA
    GAATATGCTGGTAAAACTTGGGAGACTTCAATAACAGATTTAGGTTTATCTAAAAATCAG
    GCATATAATTTCTTAATTACATCTAGTCAAAGATGGGGCCTTAATCAAGGGATAAATGCA
    AATGGCTGGATGAGAACTGACTTGAAAGGTTCAGAGTTTACTTTTACACCAGAAGCGCCA
    AAAACAATAACAGAATTAGAAAAAAAAGTTGAAGAGATTCCATTCAAGAAAGAACGTAAA
    TTTAATCCGGATTTAGCACCAGGGACAGAAAAAGTAACAAGAGAAGGACAAAAAGGTGAG
    AAGACAATAACGACACCAACACTAAAAAATCCATTAACTGGAGTAATTATTAGTAAAGGT
    GAACCAAAAGAAGAGATTACAAAAGATCCGATTAATGAATTAACAGAATACGGACCTGAA
    ACAATAGCGCCAGGTCATCGAGACGAATTTGATCCGAAGTTACCAACAGGAGAGAAAGAG
    GAAGTTCCAGGTAAACCAGGAATTAAGAATCCAGAAACAGGAGACGTAGTTAGACCGCCG
    GTCGATAGCGTAACAAAATATGGACCTGTAAAAGGAGACTCGATTGTAGAAAAAGAAGAG
    ATTCCATTCGAGAAAGAACGTAAATTTAATCCTGATTTAGCACCAGGGACAGAAAAAGTA
    ACAAGAGAAGGACAAAAAGGTGAGAAGACAATAACGACGCCAACACTAAAAAATCCATTA
    ACTGGAGAAATTATTAGTAAAGGTGAATCGAAAGAAGAAATCACAAAAGATCCGATTAAT
    GAATTAACAGAATACGGACCAGAAACGATAACACCAGGTCATCGAGACGAATTTGATCCG
    AAGTTACCAACAGGAGAGAAAGAGGAAGTTCCAGGTAAACCAGGAATTAAGAATCCAGAA
    ACAGGAGATGTAGTTAGACCACCGGTCGATAGCGTAACAAAATATGGACCTGTAAAAGGA
    GACTCGATTGTAGAAAAAGAAGAGATTCCATTCGAGAAAGAACGTAAATTTAATCCTGAT
    TTAGCACCAGGGACAGAAAAAGTAACAAGAGAAGGACAAAAAGGTGAGAAGACAATAACG
    ACACCAACACTAAAAAATCCATTAACTGGAGTAATTATTAGTAAAGGTGAACCAAAAGAA
    GAAATCACAAAAGATCCGATTAATGAATTAACAGAATACGGACCAGAAACGATAACACCA
    GGTCATCGAGACGAATTTGATCCGAAGTTACCAACAGGAGAGAAAGAAGAAGTTCCAGGT
    AAACCAGGAATTAAGAATCCAGAAACAGGAGACGTAGTTAGACCACCGGTCGATAGCGTA
    ACAAAATATGGACCTGTAAAAGGAGACTCGATTGTAGAAAAAGAAGAGATTCCATTCAAG
    AAAGAACGTAAATTTAATCCGGATTTAGCACCAGGGACAGAAAAAGTAACAAGAGAAGGA
    CAAAAAGGTGAGAAGACAATAACGACGCCAACACTAAAAAATCCATTAACTGGAGAAATT
    ATTAGTAAAGGTGAATCGAAAGAAGAAATCACAAAAGATCCGATTAATGAATTAACAGAA
    TACGGACCAGAAACGATAACACCAGGTCATCGAGACGAATTTGATCCGAAGTTACCAACA
    GGAGAGAAAGAGGAAGTTCCAGGTAAACCAGGAATTAAGAATCCAGAAACAGGAGATGTA
    GTTAGACCACCGGTCGATAGCGTAACAAAATATGGACCTGTAAAAGGAGACTCGATTGTA
    GAAAAAGAAGAGATTCCATTCGAGAAAGAACGTAAATTTAATCCTGATTTAGCACCAGGG
    ACAGAAAAAGTAACAAGAGAAGGACAAAAAGGTGAGAAGACAATAACGACGCCAACACTA
    AAAAATCCATTAACTGGAGAAATTATTAGTAAAGGTGAATCGAAAGAAGAAATCACAAAA
    GATCCGATTAATGAATTAACAGAATACGGACCAGAAACGATAACACCAGGTCATCGAGAC
    GAATTTGATCCGAAGTTACCAACAGGAGAGAAAGAGGAAGTTCCAGGTAAACCAGGAATT
    AAGAATCCAGAAACAGGAGACGTAGTTAGACCACCGGTCGATAGCGTAACAAAATATGGA
    CCTGTAAAAGGAGACTCGATTGTAGAAAAAGAAGAAATTCCATTCAAGAAAGAACGTAAA
    TTTAATCCTGATTTAGCACCAGGGACAGAAAAAGTAACAAGAGAAGGACAAAAAGGTGAG
    AAGACAATAACGACGCCAACACTAAAAAATCCATTAACTGGAGAAATTATTAGTAAAGGT
    GAATCGAAAGAAGAAATCACAAAAGATCCGATTAATGAATTAACAGAATACGGACCAGAA
    ACGATAACACCAGGTCATCGAGACGAATTTGATCCGAAGTTACCAACAGGAGAGAAAGAG
    GAAGTTCCAGGTAAACCAGGAATTAAGAATCCAGAAACAGGAGATGTAGTTAGACCACCG
    GTCGATAGCGTAACAAAATATGGACCTGTAAAAGGAGACTCGATTGTAGAAAAAGAAGAA
    ATTCCATTCGAGAAAGAACGTAAATTTAATCCTGATTTAGCACCAGGGACAGAAAAAGTA
    ACAAGAGAAGGACAAAAAGGTGAGAAGACAATAACGACGCCAACACTAAAAAATCCATTA
    ACTGGAGAAATTATTAGTAAAGGTGAATCGAAAGAAGAAATCACAAAAGATCCGATTAAT
    GAATTAACAGAATACGGACCAGAAACGATAACACCAGGTCATCGAGACGAATTTGATCCG
    AAGTTACCAACAGGAGAGAAAGAGGAAGTTCCAGGTAAACCAGGAATTAAGAATCCAGAA
    ACAGGAGATGTAGTTAGACCACCGGTCGATAGCGTAACAAAATATGGACCTGTAAAAGGA
    GACTCGATTGTAGAAAAAGAAGAAATTCCATTCGAGAAAGAACGTAAATTTAATCCTGAT
    TTAGCACCAGGGACAGAAAAAGTAACAAGAGAAGGACAAAAAGGTGAGAAGACAATAACG
    ACGCCAACACTAAAAAATCCATTAACTGGAGAAATTATTAGTAAAGGTGAATCGAAAGAA
    GAAATCACAAAAGATCCAGTTAATGAATTAACAGAATTCGGTGGCGAGAAAATACCGCAA
    GGTCATAAAGATATCTTTGATCCAAACTTACCAACAGATCAAACGGAAAAAGTACCAGGT
    AAACCAGGAATCAAGAATCCAGACACAGGAAAAGTGATCGAAGAGCCAGTGGATGATGTG
    ATTAAACACGGACCAAAAACGGGTACACCAGAAACAAAAACAGTAGAGATACCGTTTGAA
    ACAAAACGTGAGTTTAATCCAAAATTACAACCTGGTGAAGAGCGAGTGAAACAAGAAGGA
    CAACCAGGAAGTAAGACAATCACAACACCAATCACAGTGAACCCATTAACAGGTGAAAAA
    GTTGGCGAGGGTCAACCAACAGAAGAGATCACAAAACAACCAGTAGATAAGATTGTAGAG
    TTCGGTGGAGAGAAACCAAAAGATCCAAAAGGACCTGAAAACCCAGAGAAGCCGAGCAGA
    CCAACTCATCCAAGTGGCCCAGTAAATCCTAACAATCCAGGATTATCGAAAGACAGAGCA
    AAACCAAATGGCCCAGTTCATTCAATGGATAAAAATGATAAAGTTAAAAAATCTAAAATT
    GCTAAAGAATCAGTAGCTAATCAAGAGAAAAAACGAGCAGAATTACCAAAAACAGGTTTA
    GAAAGCACGCAAAAAGGTTTGATCTTTAGTAGTATAATTGGAATTGCTGGATTAATGTTA
    TTGGCTCGTAGAAGAAAGAATTAA
    60
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    960
    1020
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    2160
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    2700
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    3660
    3720
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    3900
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    4020
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    4140
    4200
    4260
    4320
    4380
    4440
    4500
    4560
    4620
    4680
    4740
    4800
    4860
    4884

Protein[edit source | edit]

Protein Data Bank: 3TIP
Protein Data Bank: 3TIQ
Protein Data Bank: 4WVE
Protein Data Bank: 5DBL

General[edit source | edit]

  • locus tag: SAOUHSC_02798
  • symbol: SAOUHSC_02798
  • description: hypothetical protein
  • length: 1627
  • theoretical pI: 5.08263
  • theoretical MW: 178526
  • GRAVY: -0.986663

Function[edit source | edit]

  • reaction:
  • TIGRFAM:
    calcium-translocating P-type ATPase, PMCA-type (TIGR01517; EC 3.6.3.8; HMM-score: 66)
    gram-positive signal peptide, YSIRK family (TIGR01168; HMM-score: 46)
    Cell structureCell envelopeOtherLPXTG cell wall anchor domain (TIGR01167; HMM-score: 23.5)
    zinc metalloproteinase 18-residue repeat (TIGR04227; HMM-score: 8.3)
  • TheSEED:  
    Virulence, Disease and DefenseAdhesionAdhesins in Staphylococcus Virulence-associated cell-wall-anchored protein SasG (LPXTG motif), binding to squamous nasal epithelial cells 
  • PFAM:
    G5 (CL0593) SasG_E; E domain (PF17041; HMM-score: 731.7)
    G5; G5 domain (PF07501; HMM-score: 354.2)
    no clan definedYSIRK_signal; YSIRK type signal peptide (PF04650; HMM-score: 44)
    DMP12; DNA-mimic protein (PF16779; HMM-score: 36.2)
    Ribosomal_L28; Ribosomal L28 family (PF00830; HMM-score: 30.3)
    NTF2 (CL0051) DUF3828; Protein of unknown function (DUF3828) (PF12883; HMM-score: 29.2)
    no clan definedGram_pos_anchor; LPXTG cell wall anchor motif (PF00746; HMM-score: 24.8)
    2C_adapt; 2-cysteine adaptor domain (PF08793; HMM-score: 19.5)

Structure, modifications & interactions[edit source | edit]

  • domains:
  • modifications:
  • cofactors:
  • effectors:
  • protein partners:

Localization[edit source | edit]

  • PSORTb: Cellwall
    • Cytoplasmic Score: 0
    • Cytoplasmic Membrane Score: 0
    • Cellwall Score: 10
    • Extracellular Score: 0
    • Internal Helices: 0
  • LocateP: LPxTG Cell-wall anchored
    • Prediction by SwissProt Classification: Cell Wall
    • Pathway Prediction: Sec-(SPI)
    • Intracellular Possibility: 0.5
    • Signal Peptide Possibility: -0.5
    • N-terminally Anchored Score: 3
    • Predicted Cleavage Site: found LPxTG motif :LPKTG
  • SignalP: Signal peptide length 51 aa
    • Ymax: 0.626
    • Ymax_pos: 51
    • Cmax: 0.66
    • Cmax_pos: 51
    • Smax: 0.722
    • Smax_pos: 47
    • Smean: 0.345
    • D: 0.517
  • predicted transmembrane helices (TMHMM): 0

Accession numbers[edit source | edit]

Protein sequence[edit source | edit]

  • MRDKKGPVNKRVDFLSNKLNKYSIRKFTVGTASILIGSLMYLGTQQEAEAAENNIENPTTLKDNVQSKEVKIEEVTNKDTAPQGVEAKSEVTSNKDTIEHEPSVKAEDISKKEDTPKEVADVAEVQPKSSVTHNAETPKVRKARSVDEGSFDITRDSKNVVESTPITIQGKEHFEGYGSVDIQKKPTDLGVSEVTRFNVGNESNGLIGALQLKNKIDFSKDFNFKVRVANNHQSNTTGADGWGFLFSKGNAEEYLTNGGILGDKGLVNSGGFKIDTGYIYTSSMDKTEKQAGQGYRGYGAFVKNDSSGNSQMVGENIDKSKTNFLNYADNSTNTSDGKFHGQRLNDVILTYVASTGKMRAEYAGKTWETSITDLGLSKNQAYNFLITSSQRWGLNQGINANGWMRTDLKGSEFTFTPEAPKTITELEKKVEEIPFKKERKFNPDLAPGTEKVTREGQKGEKTITTPTLKNPLTGVIISKGEPKEEITKDPINELTEYGPETIAPGHRDEFDPKLPTGEKEEVPGKPGIKNPETGDVVRPPVDSVTKYGPVKGDSIVEKEEIPFEKERKFNPDLAPGTEKVTREGQKGEKTITTPTLKNPLTGEIISKGESKEEITKDPINELTEYGPETITPGHRDEFDPKLPTGEKEEVPGKPGIKNPETGDVVRPPVDSVTKYGPVKGDSIVEKEEIPFEKERKFNPDLAPGTEKVTREGQKGEKTITTPTLKNPLTGVIISKGEPKEEITKDPINELTEYGPETITPGHRDEFDPKLPTGEKEEVPGKPGIKNPETGDVVRPPVDSVTKYGPVKGDSIVEKEEIPFKKERKFNPDLAPGTEKVTREGQKGEKTITTPTLKNPLTGEIISKGESKEEITKDPINELTEYGPETITPGHRDEFDPKLPTGEKEEVPGKPGIKNPETGDVVRPPVDSVTKYGPVKGDSIVEKEEIPFEKERKFNPDLAPGTEKVTREGQKGEKTITTPTLKNPLTGEIISKGESKEEITKDPINELTEYGPETITPGHRDEFDPKLPTGEKEEVPGKPGIKNPETGDVVRPPVDSVTKYGPVKGDSIVEKEEIPFKKERKFNPDLAPGTEKVTREGQKGEKTITTPTLKNPLTGEIISKGESKEEITKDPINELTEYGPETITPGHRDEFDPKLPTGEKEEVPGKPGIKNPETGDVVRPPVDSVTKYGPVKGDSIVEKEEIPFEKERKFNPDLAPGTEKVTREGQKGEKTITTPTLKNPLTGEIISKGESKEEITKDPINELTEYGPETITPGHRDEFDPKLPTGEKEEVPGKPGIKNPETGDVVRPPVDSVTKYGPVKGDSIVEKEEIPFEKERKFNPDLAPGTEKVTREGQKGEKTITTPTLKNPLTGEIISKGESKEEITKDPVNELTEFGGEKIPQGHKDIFDPNLPTDQTEKVPGKPGIKNPDTGKVIEEPVDDVIKHGPKTGTPETKTVEIPFETKREFNPKLQPGEERVKQEGQPGSKTITTPITVNPLTGEKVGEGQPTEEITKQPVDKIVEFGGEKPKDPKGPENPEKPSRPTHPSGPVNPNNPGLSKDRAKPNGPVHSMDKNDKVKKSKIAKESVANQEKKRAELPKTGLESTQKGLIFSSIIGIAGLMLLARRRKN

Experimental data[edit source | edit]

Expression & Regulation[edit source | edit]

Operon[edit source | edit]

Regulation[edit source | edit]

  • sigma factor:
  • regulator:

Transcription pattern[edit source | edit]

Protein synthesis (provided by Aureolib)[edit source | edit]

Protein stability[edit source | edit]

  • half-life: no data available

Biological Material[edit source | edit]

Mutants[edit source | edit]

Expression vector[edit source | edit]

lacZ fusion[edit source | edit]

GFP fusion[edit source | edit]

two-hybrid system[edit source | edit]

FLAG-tag construct[edit source | edit]

Antibody[edit source | edit]

Other Information[edit source | edit]

You are kindly invited to share additional interesting facts.

Literature[edit source | edit]

References[edit source | edit]

  1. Stephan Michalik, Maren Depke, Annette Murr, Manuela Gesell Salazar, Ulrike Kusebauch, Zhi Sun, Tanja C Meyer, Kristin Surmann, Henrike Pförtner, Petra Hildebrandt, Stefan Weiss, Laura Marcela Palma Medina, Melanie Gutjahr, Elke Hammer, Dörte Becher, Thomas Pribyl, Sven Hammerschmidt, Eric W Deutsch, Samuel L Bader, Michael Hecker, Robert L Moritz, Ulrike Mäder, Uwe Völker, Frank Schmidt
    A global Staphylococcus aureus proteome resource applied to the in vivo characterization of host-pathogen interactions.
    Sci Rep: 2017, 7(1);9718
    [PubMed:28887440] [WorldCat.org] [DOI] (I e)
  2. Maren Depke, Stephan Michalik, Alexander Rabe, Kristin Surmann, Lars Brinkmann, Nico Jehmlich, Jörg Bernhardt, Michael Hecker, Bernd Wollscheid, Zhi Sun, Robert L Moritz, Uwe Völker, Frank Schmidt
    A peptide resource for the analysis of Staphylococcus aureus in host-pathogen interaction studies.
    Proteomics: 2015, 15(21);3648-3661
    [PubMed:26224020] [WorldCat.org] [DOI] (I p)
  3. Ulrike Mäder, Pierre Nicolas, Maren Depke, Jan Pané-Farré, Michel Debarbouille, Magdalena M van der Kooi-Pol, Cyprien Guérin, Sandra Dérozier, Aurelia Hiron, Hanne Jarmer, Aurélie Leduc, Stephan Michalik, Ewoud Reilman, Marc Schaffer, Frank Schmidt, Philippe Bessières, Philippe Noirot, Michael Hecker, Tarek Msadek, Uwe Völker, Jan Maarten van Dijl
    Staphylococcus aureus Transcriptome Architecture: From Laboratory to Infection-Mimicking Conditions.
    PLoS Genet.: 2016, 12(4);e1005962
    [PubMed:27035918] [WorldCat.org] [DOI] (I e)

Relevant publications[edit source | edit]

Fiona M Roche, Ruth Massey, Sharon J Peacock, Nicholas P J Day, Livia Visai, Pietro Speziale, Alex Lam, Mark Pallen, Timothy J Foster
Characterization of novel LPXTG-containing proteins of Staphylococcus aureus identified from genome sequences.
Microbiology (Reading, Engl.): 2003, 149(Pt 3);643-54
[PubMed:12634333] [WorldCat.org] [DOI] (P p)
Rebecca M Corrigan, David Rigby, Pauline Handley, Timothy J Foster
The role of Staphylococcus aureus surface protein SasG in adherence and biofilm formation.
Microbiology (Reading, Engl.): 2007, 153(Pt 8);2435-46
[PubMed:17660408] [WorldCat.org] [DOI] (P p)
Dominika T Gruszka, Justyna A Wojdyla, Richard J Bingham, Johan P Turkenburg, Iain W Manfield, Annette Steward, Andrew P Leech, Joan A Geoghegan, Timothy J Foster, Jane Clarke, Jennifer R Potts
Staphylococcal biofilm-forming protein has a contiguous rod-like structure.
Proc. Natl. Acad. Sci. U.S.A.: 2012, 109(17);E1011-8
[PubMed:22493247] [WorldCat.org] [DOI] (I p)