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04-0298108BA0217611819-9771193COLECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50N315NCTC8325NewmanRF122ST398T0131TCH60TW20USA300_FPR3757USA300_TCH1516VC40

NCBI: 27-JUN-2013

Summary[edit source | edit]

  • organism: Staphylococcus aureus N315
  • locus tag: SAP001 [new locus tag: SA_RS00005 ]
  • pan locus tag?:
  • symbol: rep
  • pan gene symbol?:
  • synonym:
  • product: hypothetical protein

Genome View[edit source | edit]

Gene[edit source | edit]

General[edit source | edit]

  • type: CDS
  • locus tag: SAP001 [new locus tag: SA_RS00005 ]
  • symbol: rep
  • product: hypothetical protein
  • replicon: pN315
  • strand: +
  • coordinates: 232..1215
  • length: 984
  • essential: unknown

Accession numbers[edit source | edit]

Phenotype[edit source | edit]

Share your knowledge and add information here. [edit]

DNA sequence[edit source | edit]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    ATGCAAAATCAATATTTTACAGTGCAAGAAAATTACAAAGAAAGATTTTATCAAATCCCT
    AAAGTATTCTTTACCAGTGAAAATTATAAAAATCTAACTAATGATATGAAAATAGCCTAT
    GCTATCTTACGTGATCGCTTAAATTTATCTATTAAAAATAGCTGGGTTGATGAAGATGGT
    AACATTTACTTTGTTTATTCAAATGAAAAGCTAATGGAAATCTTAAATTGTAAAAAAGAA
    AAGTTAACAAAAATAAAAAAAGGGTTAGAAAACGATGGTCTACTCATTCAAAAACGACGT
    GGTTTAAATAAACCAAATATTCTATATTTAATGAAACCTATTGTCACAGAACGTGATATT
    TACAAAATAGAAAAAGAAGAAAACGATGTTGAACCTTACGGCGAGAAGGACGTTCGATTT
    TCGAACGTCCAGAAGTTCGAAAATCGAACGTCCAGAAGTTCGAAAATCGAACGTCCAGAA
    GTTCGAAAATCGAACACTAATGATACTGACTTTAATGACATTGACTTTAATGATACTGAG
    AATAATGATATGAATGACTTGAATGATATAAAGTACAAAAATGAAATTAGTAATCATTCA
    AATCATTCAAATCAATTTACACATAATTTTGATGATAAAGAAATGCTATTGCAAGAATTC
    CCAGAACAACTAACTAATTATTTATTAAAATATGATTATAGAGATTTAGAAATCATAAAG
    GCTGTTATTCTTAAAGCAAAAAAATCATTTAATTCAAGGCATGAAGATATGCATTACATG
    TTAGAGGACATTGAGGATGAAATATTAACTTCTTTAAAAAGACTTAAAAAAGCTATACAT
    GATAGGGGAGTTAAAGGCCAAAAGGAAACAATCAAGTCAATGCAAGCTTATTTAATGCAA
    ACAATTCTAACTGAACTTGAAGAAACACATGCCCTTTATATGCGCCGTAAAAACATGAAA
    CAATATAATATCTTTAATCAATAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    984

Protein[edit source | edit]

General[edit source | edit]

  • locus tag: SAP001 [new locus tag: SA_RS00005 ]
  • symbol: Rep
  • description: hypothetical protein
  • length: 327
  • theoretical pI: 7.2328
  • theoretical MW: 39366.4
  • GRAVY: -0.9737

Function[edit source | edit]

  • TIGRFAM:
  • TheSEED:  
    Replication initiation protein A 
  • PFAM:
    HTH (CL0123) RepA_N; Replication initiator protein A (RepA) N-terminus (PF06970; HMM-score: 107.1)
    and 1 more
    HTH_36; Helix-turn-helix domain (PF13730; HMM-score: 13.8)

Structure, modifications & interactions[edit source | edit]

  • domains:
  • modifications:
  • cofactors:
  • effectors:
  • protein partners:

Localization[edit source | edit]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 7.5
    • Cytoplasmic Membrane Score: 1.15
    • Cellwall Score: 0.62
    • Extracellular Score: 0.73
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular Possibility: 1
    • Signal Peptide Possibility: -1
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • Ymax: 0.13
    • Ymax_pos: 13
    • Cmax: 0.119
    • Cmax_pos: 42
    • Smax: 0.223
    • Smax_pos: 9
    • Smean: 0.171
    • D: 0.146
  • predicted transmembrane helices (TMHMM): 0

Accession numbers[edit source | edit]

Protein sequence[edit source | edit]

  • MQNQYFTVQENYKERFYQIPKVFFTSENYKNLTNDMKIAYAILRDRLNLSIKNSWVDEDGNIYFVYSNEKLMEILNCKKEKLTKIKKGLENDGLLIQKRRGLNKPNILYLMKPIVTERDIYKIEKEENDVEPYGEKDVRFSNVQKFENRTSRSSKIERPEVRKSNTNDTDFNDIDFNDTENNDMNDLNDIKYKNEISNHSNHSNQFTHNFDDKEMLLQEFPEQLTNYLLKYDYRDLEIIKAVILKAKKSFNSRHEDMHYMLEDIEDEILTSLKRLKKAIHDRGVKGQKETIKSMQAYLMQTILTELEETHALYMRRKNMKQYNIFNQ

Experimental data[edit source | edit]

  • experimentally validated: no data available

Expression & Regulation[edit source | edit]

Operon[edit source | edit]

Regulation[edit source | edit]

  • sigma factor:
  • regulator:

Transcription pattern[edit source | edit]

Protein synthesis (provided by Aureolib)[edit source | edit]

Protein stability[edit source | edit]

  • half-life: no data available

Biological Material[edit source | edit]

Mutants[edit source | edit]

Expression vector[edit source | edit]

lacZ fusion[edit source | edit]

GFP fusion[edit source | edit]

two-hybrid system[edit source | edit]

FLAG-tag construct[edit source | edit]

Antibody[edit source | edit]

Other Information[edit source | edit]

You are kindly invited to share additional interesting facts.

Literature[edit source | edit]

References[edit source | edit]

Relevant publications[edit source | edit]