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PangenomeCOLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 02-MAR-2017

Summary[edit | edit source]

  • organism: Staphylococcus aureus USA300_FPR3757
  • locus tag: SAUSA300_RS00370 [old locus tag: SAUSA300_0073 ]
  • pan locus tag?: SAUPAN000821000
  • symbol: SAUSA300_RS00370
  • pan gene symbol?:
  • synonym:
  • product: nickel ABC transporter substrate-binding protein

Genome View[edit | edit source]

Gene[edit | edit source]

General[edit | edit source]

  • type: CDS
  • locus tag: SAUSA300_RS00370 [old locus tag: SAUSA300_0073 ]
  • symbol: SAUSA300_RS00370
  • product: nickel ABC transporter substrate-binding protein
  • replicon: chromosome
  • strand: +
  • coordinates: 80394..81947
  • length: 1554
  • essential: unknown

Accession numbers[edit | edit source]

Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    ATGAGGAAATGTGTATTAATGACGGTAGCAGCAAGTGCTACGCTCTTATTGGCAGGTTGT
    GGCAATGGTCAAAAAGAAGATAAAGATGTTACGGTATCGCTACCTACTGAAGCAAAGGCG
    GATAAACTTGACGCGCAAGGCTATGATGCAGCGATGCCCGTTTATAGTGCAGTGTATGAT
    GCATTAGTTAAATATGATAAAGATAAGGGTATTAAAGCAGGTTTAGCAGATAAATGGAGC
    GTTGATGAATCAGGGAAAGTTTATGAATTCCATTTGAAAAAGAATGTTAAATTCTCAGAT
    GGTTCAGCATTAGATGCTAAGGACGTGAAATTCTCGATTGATCGTGCGAAAGCGATGAAC
    AAAGATTCGACTGTAGAAACGTTAAAAAAATTAGATAAGGTCGTTGTTAAAAATGAGCAC
    GTGGTCCAAATTAGATTGAAATCTCCTTCAAATCAAGTGTTAAATGAATTAACACAAGTG
    AGACCGTTGCGTATTATGAGTCCACATTCAGTAGAAGATGGTAAAGTAAACGGTAAATTT
    GAAAAAGCGATTGGAACAGGTGCATTTGTTGTTGATAAAACTGGTAAAGAAAAAACGACA
    ATGAAGCCAAATAAATATTTTGACAACGGTCACCCAGTCAATTATCATCTTGCATTCCAA
    ACGATTGAAGATGGGGACTCAAGAAATTCTGCAGTACAAAGTGGTTCTGTAGATATTTCT
    GGTGGTGCTTTAGGTATGCTCTCAGACGAACAAATCAAACAAGATAAGAAAAATAAGAAC
    TTAACGATTGAAGATAGACCTAGCACAGTAAGTCACTTTATGGCATTTAACCCTAAAAAT
    GATGTATTAAATCAACGCACAATTCGTGAAGCGATAAGTAAGAGCATCGATGCGAAAGAC
    ATTGCGGGCAAATCTGTAAATGGTCTGTTCCAGAAGAACGTACAATTTGTGACTAAAAAT
    AATCAACAGCCACACGATTATGATATGAAAGCGGCTGAAAGGTTACTTAAATCAGAAGGA
    TATCATAAAAACGATGACGGCATCTTTGAAAAGAATGGCAAACCTTTATCATTTAACTTA
    GTCATTCAAACTGCAGAGTTCCCAAATTGGAAAGATAAAGCTGAAAAAGTGCAACGTCAG
    CTTAAACAAGCCGGTATTAAGTTAAATGTGAAAACGTTAGATTCACAATCATACTATGAT
    ACATTATGGACGAAAAAAGACTATGATTTGATTTTCTATAGAACGTATTCAGATGCATTA
    ATGCCTTACAACTTTATAAGTTCAGTGTTTAAAAATAATGATGGTCAACCAGGGGTGTTA
    GCTGATGATGAAACATTAACGAAACAGCTAGACGATTTCCCATCAACCGTATCAAAAGAA
    GACCAACAGTGTTCATTTGATGACATATTTAAACACTTTAATCAACAATACTATGGTGTG
    CCAATTGCTTATCCAAATGAGACGTTTGTAGTGAGTGATAAAGTAAAACAATTCAAATTC
    TCTGGACTTACGGATGCACCAATTGATTATAAAGCGTTGAAAGTTAATGAATAG
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1200
    1260
    1320
    1380
    1440
    1500
    1554

Protein[edit | edit source]

General[edit | edit source]

  • locus tag: SAUSA300_RS00370 [old locus tag: SAUSA300_0073 ]
  • symbol: SAUSA300_RS00370
  • description: nickel ABC transporter substrate-binding protein
  • length: 517
  • theoretical pI: 8.58302
  • theoretical MW: 57943.1
  • GRAVY: -0.62824

Function[edit | edit source]

  • TIGRFAM:
    Metabolism Transport and binding proteins Cations and iron carrying compounds nickel ABC transporter, nickel/metallophore periplasmic binding protein (TIGR02294; HMM-score: 230.6)
    and 1 more
    Metabolism Transport and binding proteins Unknown substrate ABC transporter substrate binding protein, KPN_01854 family (TIGR04028; HMM-score: 94.8)
  • TheSEED: see SAUSA300_0073
  • PFAM:
    PBP (CL0177) SBP_bac_5; Bacterial extracellular solute-binding proteins, family 5 Middle (PF00496; HMM-score: 232.6)
    and 1 more
    LppaM (CL0421) LPAM_2; Prokaryotic lipoprotein-attachment site (PF13627; HMM-score: 10.3)

Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

Localization[edit | edit source]

  • PSORTb: Cellwall
    • Cytoplasmic Score: 0
    • Cytoplasmic Membrane Score: 0.01
    • Cellwall Score: 9.21
    • Extracellular Score: 0.78
    • Internal Helices: 0
  • LocateP:
  • SignalP: Signal peptide LIPO(Sec/SPII) length 19 aa
    • SP(Sec/SPI): 0.000344
    • TAT(Tat/SPI): 0.000043
    • LIPO(Sec/SPII): 0.999509
    • Cleavage Site: CS pos: 19-20. LAG-CG. Pr: 0.9999
  • predicted transmembrane helices (TMHMM): 0

Accession numbers[edit | edit source]

Protein sequence[edit | edit source]

  • MRKCVLMTVAASATLLLAGCGNGQKEDKDVTVSLPTEAKADKLDAQGYDAAMPVYSAVYDALVKYDKDKGIKAGLADKWSVDESGKVYEFHLKKNVKFSDGSALDAKDVKFSIDRAKAMNKDSTVETLKKLDKVVVKNEHVVQIRLKSPSNQVLNELTQVRPLRIMSPHSVEDGKVNGKFEKAIGTGAFVVDKTGKEKTTMKPNKYFDNGHPVNYHLAFQTIEDGDSRNSAVQSGSVDISGGALGMLSDEQIKQDKKNKNLTIEDRPSTVSHFMAFNPKNDVLNQRTIREAISKSIDAKDIAGKSVNGLFQKNVQFVTKNNQQPHDYDMKAAERLLKSEGYHKNDDGIFEKNGKPLSFNLVIQTAEFPNWKDKAEKVQRQLKQAGIKLNVKTLDSQSYYDTLWTKKDYDLIFYRTYSDALMPYNFISSVFKNNDGQPGVLADDETLTKQLDDFPSTVSKEDQQCSFDDIFKHFNQQYYGVPIAYPNETFVVSDKVKQFKFSGLTDAPIDYKALKVNE

Experimental data[edit | edit source]

  • experimentally validated:
  • protein localization:
  • quantitative data / protein copy number per cell:
  • interaction partners:

Expression & Regulation[edit | edit source]

Operon[edit | edit source]

Regulation[edit | edit source]

  • regulator:

Transcription pattern[edit | edit source]

Protein synthesis (provided by Aureolib)[edit | edit source]

Protein stability[edit | edit source]

  • half-life: no data available

Biological Material[edit | edit source]

Mutants[edit | edit source]

Expression vector[edit | edit source]

lacZ fusion[edit | edit source]

GFP fusion[edit | edit source]

two-hybrid system[edit | edit source]

FLAG-tag construct[edit | edit source]

Antibody[edit | edit source]

Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

Literature[edit | edit source]

References[edit | edit source]

Relevant publications[edit | edit source]