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Pangenome04-0298108BA0217611819-9771193COLECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50N315NCTC8325NewmanRF122ST398T0131TCH60TW20USA300_FPR3757USA300_TCH1516VC40
NCBI: 02-MAR-2017

Summary[edit source | edit]

  • organism: Staphylococcus aureus USA300_FPR3757
  • locus tag: SAUSA300_RS00370 [old locus tag: SAUSA300_0073 ]
  • pan locus tag?: SAUPAN000821000
  • symbol: SAUSA300_RS00370
  • pan gene symbol?:
  • synonym:
  • product: nickel ABC transporter substrate-binding protein

Genome View[edit source | edit]

Gene[edit source | edit]

General[edit source | edit]

  • type: CDS
  • locus tag: SAUSA300_RS00370 [old locus tag: SAUSA300_0073 ]
  • symbol: SAUSA300_RS00370
  • product: nickel ABC transporter substrate-binding protein
  • replicon: chromosome
  • strand: +
  • coordinates: 80394..81947
  • length: 1554
  • essential: unknown

Accession numbers[edit source | edit]

  • Location: NC_007793 (80394..81947) NCBI
  • MicrobesOnline:

Phenotype[edit source | edit]

  • Share your knowledge and add information here. [edit]

DNA sequence[edit source | edit]

  • 1
    61
    121
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    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    ATGAGGAAATGTGTATTAATGACGGTAGCAGCAAGTGCTACGCTCTTATTGGCAGGTTGT
    GGCAATGGTCAAAAAGAAGATAAAGATGTTACGGTATCGCTACCTACTGAAGCAAAGGCG
    GATAAACTTGACGCGCAAGGCTATGATGCAGCGATGCCCGTTTATAGTGCAGTGTATGAT
    GCATTAGTTAAATATGATAAAGATAAGGGTATTAAAGCAGGTTTAGCAGATAAATGGAGC
    GTTGATGAATCAGGGAAAGTTTATGAATTCCATTTGAAAAAGAATGTTAAATTCTCAGAT
    GGTTCAGCATTAGATGCTAAGGACGTGAAATTCTCGATTGATCGTGCGAAAGCGATGAAC
    AAAGATTCGACTGTAGAAACGTTAAAAAAATTAGATAAGGTCGTTGTTAAAAATGAGCAC
    GTGGTCCAAATTAGATTGAAATCTCCTTCAAATCAAGTGTTAAATGAATTAACACAAGTG
    AGACCGTTGCGTATTATGAGTCCACATTCAGTAGAAGATGGTAAAGTAAACGGTAAATTT
    GAAAAAGCGATTGGAACAGGTGCATTTGTTGTTGATAAAACTGGTAAAGAAAAAACGACA
    ATGAAGCCAAATAAATATTTTGACAACGGTCACCCAGTCAATTATCATCTTGCATTCCAA
    ACGATTGAAGATGGGGACTCAAGAAATTCTGCAGTACAAAGTGGTTCTGTAGATATTTCT
    GGTGGTGCTTTAGGTATGCTCTCAGACGAACAAATCAAACAAGATAAGAAAAATAAGAAC
    TTAACGATTGAAGATAGACCTAGCACAGTAAGTCACTTTATGGCATTTAACCCTAAAAAT
    GATGTATTAAATCAACGCACAATTCGTGAAGCGATAAGTAAGAGCATCGATGCGAAAGAC
    ATTGCGGGCAAATCTGTAAATGGTCTGTTCCAGAAGAACGTACAATTTGTGACTAAAAAT
    AATCAACAGCCACACGATTATGATATGAAAGCGGCTGAAAGGTTACTTAAATCAGAAGGA
    TATCATAAAAACGATGACGGCATCTTTGAAAAGAATGGCAAACCTTTATCATTTAACTTA
    GTCATTCAAACTGCAGAGTTCCCAAATTGGAAAGATAAAGCTGAAAAAGTGCAACGTCAG
    CTTAAACAAGCCGGTATTAAGTTAAATGTGAAAACGTTAGATTCACAATCATACTATGAT
    ACATTATGGACGAAAAAAGACTATGATTTGATTTTCTATAGAACGTATTCAGATGCATTA
    ATGCCTTACAACTTTATAAGTTCAGTGTTTAAAAATAATGATGGTCAACCAGGGGTGTTA
    GCTGATGATGAAACATTAACGAAACAGCTAGACGATTTCCCATCAACCGTATCAAAAGAA
    GACCAACAGTGTTCATTTGATGACATATTTAAACACTTTAATCAACAATACTATGGTGTG
    CCAATTGCTTATCCAAATGAGACGTTTGTAGTGAGTGATAAAGTAAAACAATTCAAATTC
    TCTGGACTTACGGATGCACCAATTGATTATAAAGCGTTGAAAGTTAATGAATAG
    60
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    1140
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    1554

Protein[edit source | edit]

General[edit source | edit]

  • locus tag: SAUSA300_RS00370 [old locus tag: SAUSA300_0073 ]
  • symbol: SAUSA300_RS00370
  • description: nickel ABC transporter substrate-binding protein
  • length: 517
  • theoretical pI: 8.58302
  • theoretical MW: 57943.1
  • GRAVY: -0.62824

Function[edit source | edit]

  • reaction:
  • TIGRFAM:
    MetabolismTransport and binding proteinsCations and iron carrying compoundsnickel ABC transporter, nickel/metallophore periplasmic binding protein (TIGR02294; HMM-score: 230.6)
    MetabolismTransport and binding proteinsUnknown substrateABC transporter substrate binding protein, KPN_01854 family (TIGR04028; HMM-score: 94.8)
  • TheSEED:
  • PFAM:
    PBP (CL0177) SBP_bac_5; Bacterial extracellular solute-binding proteins, family 5 Middle (PF00496; HMM-score: 232.6)
    LppaM (CL0421) LPAM_2; Prokaryotic lipoprotein-attachment site (PF13627; HMM-score: 10.3)

Structure, modifications & interactions[edit source | edit]

  • domains:
  • modifications:
  • cofactors:
  • effectors:
  • protein partners:

Localization[edit source | edit]

  • PSORTb: Cellwall
    • Cytoplasmic Score: 0
    • Cytoplasmic Membrane Score: 0.01
    • Cellwall Score: 9.21
    • Extracellular Score: 0.78
    • Internal Helices: 0
  • LocateP:
  • SignalP: Signal peptide length 26 aa
    • Ymax: 0.33
    • Ymax_pos: 26
    • Cmax: 0.16
    • Cmax_pos: 26
    • Smax: 0.946
    • Smax_pos: 6
    • Smean: 0.776
    • D: 0.504
  • predicted transmembrane helices (TMHMM): 0

Accession numbers[edit source | edit]

  • GI: 447146920 NCBI
  • RefSeq: WP_001224176 NCBI
  • UniProt:

Protein sequence[edit source | edit]

  • MRKCVLMTVAASATLLLAGCGNGQKEDKDVTVSLPTEAKADKLDAQGYDAAMPVYSAVYDALVKYDKDKGIKAGLADKWSVDESGKVYEFHLKKNVKFSDGSALDAKDVKFSIDRAKAMNKDSTVETLKKLDKVVVKNEHVVQIRLKSPSNQVLNELTQVRPLRIMSPHSVEDGKVNGKFEKAIGTGAFVVDKTGKEKTTMKPNKYFDNGHPVNYHLAFQTIEDGDSRNSAVQSGSVDISGGALGMLSDEQIKQDKKNKNLTIEDRPSTVSHFMAFNPKNDVLNQRTIREAISKSIDAKDIAGKSVNGLFQKNVQFVTKNNQQPHDYDMKAAERLLKSEGYHKNDDGIFEKNGKPLSFNLVIQTAEFPNWKDKAEKVQRQLKQAGIKLNVKTLDSQSYYDTLWTKKDYDLIFYRTYSDALMPYNFISSVFKNNDGQPGVLADDETLTKQLDDFPSTVSKEDQQCSFDDIFKHFNQQYYGVPIAYPNETFVVSDKVKQFKFSGLTDAPIDYKALKVNE

Experimental data[edit source | edit]

  • experimentally validated: no data available

Expression & Regulation[edit source | edit]

Operon[edit source | edit]

  • SAUSA300_RS00370 no polycistronic organisation predicted

Regulation[edit source | edit]

  • sigma factor:
  • regulator:

Transcription pattern[edit source | edit]

Protein synthesis (provided by Aureolib)[edit source | edit]

Protein stability[edit source | edit]

  • half-life: no data available

Biological Material[edit source | edit]

Mutants[edit source | edit]

Expression vector[edit source | edit]

lacZ fusion[edit source | edit]

GFP fusion[edit source | edit]

two-hybrid system[edit source | edit]

FLAG-tag construct[edit source | edit]

Antibody[edit source | edit]

Other Information[edit source | edit]

You are kindly invited to share additional interesting facts.

Literature[edit source | edit]

References[edit source | edit]

Relevant publications[edit source | edit]