NCBI: 02-MAR-2017

⊟Summary

• organism: Staphylococcus aureus USA300_FPR3757
• locus tag: SAUSA300_RS03150 [old locus tag: SAUSA300_0587 ]
• pan locus tag^?: SAUPAN002444000
• symbol: SAUSA300_RS03150
• pan gene symbol^?: —
• synonym:
• product: DUF443 domain-containing protein

⊟Gene

⊟General

• type: CDS
• locus tag: SAUSA300_RS03150 [old locus tag: SAUSA300_0587 ]
• symbol: SAUSA300_RS03150
• product: DUF443 domain-containing protein
• replicon: chromosome
• strand: +
• coordinates: 660995..661630
• length: 636
• essential: unknown

⊟Accession numbers

• Location: NC_007793 (660995..661630) NCBI
• MicrobesOnline:

⊟Phenotype

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⊟DNA sequence

• 1
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  481
  541
  601
  
  TTGCTATGTGAATCTAAAGTTATCAACAAAAATCCTAAGTATAGAGTTATTAAATATGGT
  GATGAATATTTAATGATTGATTTAGTAAGTACCTGGTTAACTTTATTTCTTCCTATGATT
  AATTGGTTAATTCCAAAAAAATATGTCAAAATCAGTAAAAAAGAATTTGACGATTTAAAC
  ATTGTCAAACCTGTTAAAAATAAAGCTTTTTGGCCAGTTGCAGGTAGTACTATTTTGTTC
  GGAGTTACGTTTAGAAAATATATTCCTTCACTTAATATTCAATTAGAGAAAAACATGGTG
  ATTGTAATATGTTGTGCAATATTTCTGGGTGTTTTAATACTTTTTTTATTTCTGAATCGT
  AAGCTAAGGTTGGAAATTTATAATAATAACTCTAGTAAAGGGAAAATAATTTTATTTCCT
  TCATTAAAAAACTTTTGTTTCACAATATTTTATTATTTTTTATTTGGCGGTCTTTCAATA
  ATGGCTCTAAGTATGTTATTAACTTTAAATCCTCAAAATATAATAGGCTTTATTGGTTGG
  TTGGTAATGACTGCAGGTTTCTTTCTGTTAAACATGTCATCGATTATTGACAAAAAAATT
  TATGTATTATCTAAAACTAACACGGTGGAAAAATGA
  60
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  636
  

⊟Protein

⊟General

• locus tag: SAUSA300_RS03150 [old locus tag: SAUSA300_0587 ]
• symbol: SAUSA300_RS03150
• description: DUF443 domain-containing protein
• length: 211
• theoretical pI: 10.2473
• theoretical MW: 24428.5
• GRAVY: 0.535545

⊟Function

• reaction:
• TIGRFAM:
  tandem five-TM protein (TIGR01218; HMM-score: 174.8)
• TheSEED:
• PFAM:
  no clan definedDUF443; Protein of unknown function (DUF443) (PF04276; HMM-score: 223.4)
  DUF5357; Family of unknown function (DUF5357) (PF17310; HMM-score: 16.2)
  LtrA; Bacterial low temperature requirement A protein (LtrA) (PF06772; HMM-score: 14.1)
  Baculo_11_kDa; Baculovirus 11 kDa family (PF06143; HMM-score: 12.9)

⊟Structure, modifications & interactions

• domains:
• modifications:
• cofactors:
• effectors:
• protein partners:

⊟Localization

• PSORTb: Cytoplasmic Membrane
  • Cytoplasmic Score: 0
  • Cytoplasmic Membrane Score: 10
  • Cellwall Score: 0
  • Extracellular Score: 0
  • Internal Helices: 4
• LocateP:
• SignalP: no predicted signal peptide
  • Ymax: 0.119
  • Ymax_pos: 11
  • Cmax: 0.11
  • Cmax_pos: 52
  • Smax: 0.182
  • Smax_pos: 3
  • Smean: 0.141
  • D: 0.128
• predicted transmembrane helices (TMHMM): 5

⊟Accession numbers

• GI: 446804867 NCBI
• RefSeq: WP_000882123 NCBI
• UniProt:

⊟Protein sequence

• MLCESKVINKNPKYRVIKYGDEYLMIDLVSTWLTLFLPMINWLIPKKYVKISKKEFDDLNIVKPVKNKAFWPVAGSTILFGVTFRKYIPSLNIQLEKNMVIVICCAIFLGVLILFLFLNRKLRLEIYNNNSSKGKIILFPSLKNFCFTIFYYFLFGGLSIMALSMLLTLNPQNIIGFIGWLVMTAGFFLLNMSSIIDKKIYVLSKTNTVEK

⊟Experimental data

• experimentally validated: no data available

⊟Expression & Regulation

⊟Operon

• SAUSA300_RS03150 no polycistronic organisation predicted

⊟Regulation

• sigma factor:
• regulator:

⊟Transcription pattern

• S.aureus Expression Data Browser: no data available

⊟Protein synthesis (provided by Aureolib)

• Aureolib: no data available

⊟Protein stability

• half-life: no data available

⊟Other Information

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