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04-0298108BA0217611819-9771193COLECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50N315NCTC8325NewmanRF122ST398T0131TCH60TW20USA300_FPR3757USA300_TCH1516VC40

NCBI: 02-MAR-2017

Summary[edit source | edit]

  • organism: Staphylococcus aureus USA300_FPR3757
  • locus tag: SAUSA300_RS14805 [old locus tag: SAUSA300_pUSA030014 ]
  • pan locus tag?:
  • symbol: SAUSA300_RS14805
  • pan gene symbol?:
  • synonym:
  • product: type VI secretion protein

Genome View[edit source | edit]

Gene[edit source | edit]

General[edit source | edit]

  • type: CDS
  • locus tag: SAUSA300_RS14805 [old locus tag: SAUSA300_pUSA030014 ]
  • symbol: SAUSA300_RS14805
  • product: type VI secretion protein
  • replicon: pUSA03
  • strand: +
  • coordinates: 12505..14517
  • length: 2013
  • essential: unknown

Accession numbers[edit source | edit]

  • Location: NC_007792 (12505..14517) NCBI
  • MicrobesOnline:

Phenotype[edit source | edit]

Share your knowledge and add information here. [edit]

DNA sequence[edit source | edit]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    ATGGCATTTTTAAAAAAGAAAAAGCAAGAGCAAGTTACAAATAAAATTCACAATGAATCT
    TTTCAAAAATTAACAGAAGTATTAGATACAGATTCTGTTGATAGTATTTATCCTTTTAGT
    TGGATAGAGAAAAAATCGCATATAGAAACAGGCGAAAATTATATTAAAAATTTATTAGTA
    GTTGACTACCCTCAATCAGTAAAAGGGGCGTACTTATCAAATTTATTAAAGAAAAACGGT
    AATATTCAAATAACTAAATTTATTAGACCAGCAAATATAGAAAGAATGATTGACCATTTA
    AACAACTCAATCAAAAACAAAACAGCAGAACAAATGCGTACTACAGACCCTAAACGAAAT
    GCAACAATTAAAAGAGAAATTGAAAGTAGTAAAAAACAACTTGATAAATTTTTAGATGAA
    AAAACAGGATTTATGTACATGTATATGTATATCACGTTAAATGGCGATTCATACGAAAAG
    ATACAAGCGCTAGAAAAAGATGTTAAGCGTACTTTAACGAGATTAAGACTTAAAACACAT
    ACACCTACAAACGCAATGCGAGAATCATTTCATACAGTTCTTCCTTTAAATAGAAACTTC
    TTATCAGCATTTACACAACAAAATATGGATACAGCAACAGCAGGGCATTTCTTTATGTTT
    GATGATTCAGAAATTATTGATTTAACACCTAATACATCAGTTTTTGGAATTAACAAAAAT
    ACAGATTCATTAGTAGCAGTTGATTTTAATAACAAAGAAAAAACTTTAAATAAAAATATG
    ACTATTATCGGTACAAGTGGTGTAGGTAAATCTACCCTCAATATGAGAATGATTTTAGAC
    AATATCAAAAAATCTATAAGACAATTTATCATTGATCCCGAAGATGAATTTTCATATATA
    ACTAAATATTACGGTGGAACAGTTGTAAATATTTCAACAAGTTCAAATATCAAAATTAAT
    CCATTTGAAATTTTTTCAGAAGAAGTATTTGAAAAAGAAGATGAAACAGACAATGAAACA
    ACATCAGATGTTTTAACGGAAAACAACGAACACGAAAGCCAAATTGACACATTAGTTCGT
    TCTAAAATAGGGAAGTTAAAAACCTTCTTTAGAGTTCTTAAAGATGAAATATCTCAAACA
    GAAATATCTGTATTATCTTCAACTTTAAGACAGTTATATCAAGATAAAGGATTTAAAGGT
    AATGCGAAATTATCAGATTTCAAAAGCGAAGATTATCCAACTTTAACAGAATTATATAAC
    AAACTAAAAGACTTAGACCCAGAAAAATATGAGGTATTAAAGGACTTAACACTTATCATA
    GAAGATTACACAATGGAACATGGTACTACTACAATCTTTGACGGTTATACAAACATCAAA
    TTAGATAATGAAATTGTTACTTTTAACCTTAAACCATTACAAACAGAAAAAGATGTTCAA
    AGTGCAGCGTATTTGAATATATTCAGTTTCTTATGGGACGAAATAACAAAAGATAGAACA
    ACAGAAACAGTATTAATGACTGACGAATTACATTTCTTAGCAACAAATGAATATAGCTTA
    GATTTTTATTATCAAGCATACAAGCGTATTAGAAAATATGGCGGTGGCGCTATTGCCTCT
    ACTCAACAAATTAAAGATATTCTTAGAACATCACAAGAAATAGGTTCAGCTATTATTGAA
    AATAGTCATACTAAATTCTTCTTTGGAATGGATAACGTAGGCGTTGATGATGTTGTTGAT
    AAATTAGGATTGAAATTTAGCGATCAAGAAATATCTCATTTAACTAAAAAGAAAAAAGGC
    GAGGCATTATTACTATACGGAACACAAAGAGCATTTATAAGAATTGATTTAGATAGAGAA
    GAAACAAGACTTTGGAATAAAGAATTATATGAAACAATTTATGAAGAACCAGCAGACGTA
    GAGCCGAATTATGTTGAACAACTAGGATTAACAGATATTGATTTAGCAGAGTTGGAAGAA
    GAATTAAGACAAGCAGAAATGATTTATGAATAG
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1200
    1260
    1320
    1380
    1440
    1500
    1560
    1620
    1680
    1740
    1800
    1860
    1920
    1980
    2013

Protein[edit source | edit]

General[edit source | edit]

  • locus tag: SAUSA300_RS14805 [old locus tag: SAUSA300_pUSA030014 ]
  • symbol: SAUSA300_RS14805
  • description: type VI secretion protein
  • length: 670
  • theoretical pI: 4.91695
  • theoretical MW: 77594.2
  • GRAVY: -0.568358

Function[edit source | edit]

  • reaction:
  • TIGRFAM:
    type-IV secretion system protein TraC (TIGR02746; HMM-score: 116.9)
    type IV secretion/conjugal transfer ATPase, VirB4 family (TIGR00929; HMM-score: 100.4)
    and 3 more
    Genetic information processingMobile and extrachromosomal element functionsPlasmid functionsconjugative transfer ATPase, PFL_4706 family (TIGR03744; HMM-score: 27.2)
    MetabolismAmino acid biosynthesisAromatic amino acid familychorismate synthase (TIGR00033; EC 4.2.3.5; HMM-score: 13.9)
    MetabolismTransport and binding proteinsAmino acids, peptides and aminesglycine betaine/L-proline transport ATP binding subunit (TIGR01186; HMM-score: 13.4)
  • TheSEED:
  • PFAM:
    P-loop_NTPase (CL0023) DUF87; Domain of unknown function DUF87 (PF01935; HMM-score: 36.3)
    AAA_10; AAA-like domain (PF12846; HMM-score: 30.5)
    and 11 more
    TrwB_AAD_bind; Type IV secretion-system coupling protein DNA-binding domain (PF10412; HMM-score: 22.2)
    AAA_29; P-loop containing region of AAA domain (PF13555; HMM-score: 15.5)
    TraG-D_C; TraM recognition site of TraD and TraG (PF12696; HMM-score: 15.1)
    no clan definedMaf1; Maf1 regulator (PF09174; HMM-score: 13.2)
    P-loop_NTPase (CL0023) AAA_16; AAA ATPase domain (PF13191; HMM-score: 13.1)
    ABC_tran; ABC transporter (PF00005; HMM-score: 12.9)
    RsgA_GTPase; RsgA GTPase (PF03193; HMM-score: 12.9)
    IstB_IS21; IstB-like ATP binding protein (PF01695; HMM-score: 12.7)
    AAA_22; AAA domain (PF13401; HMM-score: 12.6)
    FtsK_SpoIIIE; FtsK/SpoIIIE family (PF01580; HMM-score: 12)
    no clan definedMlh1_C; DNA mismatch repair protein Mlh1 C-terminus (PF16413; HMM-score: 11.8)

Structure, modifications & interactions[edit source | edit]

  • domains:
  • modifications:
  • cofactors:
  • effectors:
  • protein partners:

Localization[edit source | edit]

  • PSORTb: unknown (no significant prediction)
    • Cytoplasmic Score: 2.5
    • Cytoplasmic Membrane Score: 2.5
    • Cellwall Score: 2.5
    • Extracellular Score: 2.5
    • Internal Helices: 0
  • LocateP:
  • SignalP: no predicted signal peptide
    • Ymax: 0.149
    • Ymax_pos: 12
    • Cmax: 0.111
    • Cmax_pos: 70
    • Smax: 0.277
    • Smax_pos: 2
    • Smean: 0.216
    • D: 0.176
  • predicted transmembrane helices (TMHMM): 0

Accession numbers[edit source | edit]

  • GI: 446797663 NCBI
  • RefSeq: WP_000874919 NCBI
  • UniProt:

Protein sequence[edit source | edit]

  • MAFLKKKKQEQVTNKIHNESFQKLTEVLDTDSVDSIYPFSWIEKKSHIETGENYIKNLLVVDYPQSVKGAYLSNLLKKNGNIQITKFIRPANIERMIDHLNNSIKNKTAEQMRTTDPKRNATIKREIESSKKQLDKFLDEKTGFMYMYMYITLNGDSYEKIQALEKDVKRTLTRLRLKTHTPTNAMRESFHTVLPLNRNFLSAFTQQNMDTATAGHFFMFDDSEIIDLTPNTSVFGINKNTDSLVAVDFNNKEKTLNKNMTIIGTSGVGKSTLNMRMILDNIKKSIRQFIIDPEDEFSYITKYYGGTVVNISTSSNIKINPFEIFSEEVFEKEDETDNETTSDVLTENNEHESQIDTLVRSKIGKLKTFFRVLKDEISQTEISVLSSTLRQLYQDKGFKGNAKLSDFKSEDYPTLTELYNKLKDLDPEKYEVLKDLTLIIEDYTMEHGTTTIFDGYTNIKLDNEIVTFNLKPLQTEKDVQSAAYLNIFSFLWDEITKDRTTETVLMTDELHFLATNEYSLDFYYQAYKRIRKYGGGAIASTQQIKDILRTSQEIGSAIIENSHTKFFFGMDNVGVDDVVDKLGLKFSDQEISHLTKKKKGEALLLYGTQRAFIRIDLDREETRLWNKELYETIYEEPADVEPNYVEQLGLTDIDLAELEEELRQAEMIYE

Experimental data[edit source | edit]

  • experimentally validated: no data available

Expression & Regulation[edit source | edit]

Operon[edit source | edit]

  • SAUSA300_RS14805 no polycistronic organisation predicted

Regulation[edit source | edit]

  • sigma factor:
  • regulator:

Transcription pattern[edit source | edit]

Protein synthesis (provided by Aureolib)[edit source | edit]

Protein stability[edit source | edit]

  • half-life: no data available

Biological Material[edit source | edit]

Mutants[edit source | edit]

Expression vector[edit source | edit]

lacZ fusion[edit source | edit]

GFP fusion[edit source | edit]

two-hybrid system[edit source | edit]

FLAG-tag construct[edit source | edit]

Antibody[edit source | edit]

Other Information[edit source | edit]

You are kindly invited to share additional interesting facts.

Literature[edit source | edit]

References[edit source | edit]

Relevant publications[edit source | edit]