COLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 23-MAY-2013

⊟Summary

• organism: Staphylococcus aureus USA300_FPR3757
• locus tag: SAUSA300_pUSA020001 [new locus tag: SAUSA300_RS14725 ]
• pan locus tag^?:
• symbol: SAUSA300_pUSA020001
• pan gene symbol^?: —
• synonym:
• product: tetracycline resistance protein

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: SAUSA300_pUSA020001 [new locus tag: SAUSA300_RS14725 ]
• symbol: SAUSA300_pUSA020001
• product: tetracycline resistance protein
• replicon: pUSA02
• strand: +
• coordinates: 36..1415
• length: 1380
• essential: unknown

⊟Accession numbers

• Gene ID: 3912723 NCBI
• RefSeq: YP_492684 NCBI
• BioCyc: GH3C-2671 BioCyc
• MicrobesOnline: 1291477 MicrobesOnline

⊟Phenotype

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⊟DNA sequence

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  TTGTTTAGTTTATATAAAAAATTTAAAGGTTTGTTTTATAGCGTTTTATTTTGGCTTTGT
  ATTCTTTCATTTTTTAGTGTATTAAATGAAATGGTTTTAAATGTTTCTTTACCTGATATT
  GCAAATCATTTTAATACTACTCCTGGAATTACAAACTGGGTAAACACTGCATATATGTTA
  ACTTTTTCGATAGGAACAGCAGTATATGGAAAATTATCTGATTATATAAATATAAAAAAA
  TTGTTAATTATTGGTATTAGTTTGAGCTGTCTTGGTTCATTGATTGCTTTTATTGGTCAC
  AATCACTTTTTTATTTTGATTTTTGGTAGGTTAGTACAAGGAGTAGGATCTGCTGCATTC
  CCTTCACTGATTATGGTGGTTGTAGCTAGAAATATTACAAGAAAAAAACAAGGCAAAGCC
  TTTGGTTTTATAGGATCAATTGTAGCTTTAGGTGAAGGGTTAGGTCCTTCAATAGGGGGA
  ATAATAGCACATTATATTCATTGGTCTTACCTACTTATACTTCCTATGATTACAATAGTA
  ACTATACCTTTTCTTATTAAAGTAATGGTACCTGGTAAATCAACAAAAAATACATTAGAT
  ATCGTAGGTATTGTTTTAATGTCTATAAGTATTATATGTTTTATGTTATTTACGACAAAT
  TATAATTGGACTTTTTTAATACTCTTCACAATCTTTTTTGTGATTTTTATTAAACATATT
  TCAAGAGTTTCTAACCCTTTTATTAATCCTAAACTAGGGAAAAACATTCCGTTTATGCTT
  GGTTTGTTTTCTGGTGGGCTAATATTTTCTATAGTAGCTGGTTTTATATCAATGGTGCCT
  TATATGATGAAAACTATTTATCATGTAAATGTAGCGACAATAGGTAATAGTGTTATTTTT
  CCTGGAACCATGAGTGTTATTGTTTTTGGTTATTTTGGTGGTTTTTTAGTGGATAGAAAA
  GGATCATTATTTGTTTTTATTTTAGGATCATTGTCTATCTCTATAAGTTTTTTAACTATT
  GCATTTTTTGTTGAGTTTAGTATGTGGTTGACTACTTTTATGTTTATATTTGTTATGGGC
  GGATTATCTTTTACTAAAACAGTTATATCAAAAATAGTATCAAGTAGTCTTTCTGAAGAA
  GAAGTTGCTTCTGGAATGAGTTTGCTAAATTTCACAAGTTTTTTATCAGAGGGAACAGGT
  ATAGCAATTGTAGGAGGTTTATTGTCACTACAATTGATTAATCGTAAACTAGTTCTGGAA
  TTTATAAATTATTCTTCTGGAGTGTATAGTAATATTCTTGTAGCCATGGCTATCCTTATT
  ATTTTATGTTGTCTTTTGACGATTATTGTATTTAAACGTTCTAAAAAGCAGTTTGAATAG
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⊟Protein

⊟General

• locus tag: SAUSA300_pUSA020001 [new locus tag: SAUSA300_RS14725 ]
• symbol: SAUSA300_pUSA020001
• description: tetracycline resistance protein
• length: 459
• theoretical pI: 10.1516
• theoretical MW: 50693.7
• GRAVY: 1.02026

⊟Function

• TIGRFAM:
  Cellular processes Cellular processes Toxin production and resistance drug resistance MFS transporter, drug:H+ antiporter-2 (14 Spanner) (DHA2) family (TIGR00711; HMM-score: 106.8)
  Metabolism Transport and binding proteins Other drug resistance MFS transporter, drug:H+ antiporter-2 (14 Spanner) (DHA2) family (TIGR00711; HMM-score: 106.8)
  and 4 more
  drug resistance transporter, Bcr/CflA subfamily (TIGR00710; HMM-score: 72.3)
  multidrug resistance protein (TIGR00880; HMM-score: 53.8)
  Metabolism Transport and binding proteins Carbohydrates, organic alcohols, and acids MFS transporter, aromatic acid:H+ symporter (AAHS) family (TIGR00895; HMM-score: 29)
  Metabolism Transport and binding proteins Cations and iron carrying compounds H+ Antiporter protein (TIGR00900; HMM-score: 23.7)
• TheSEED  :
  • Tetracycline resistence protein, metal-tetracycline/H+ antiporter
• PFAM:
  MFS (CL0015) MFS_1; Major Facilitator Superfamily (PF07690; HMM-score: 135.8)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors:
• effectors:

⊟Localization

• PSORTb: Cytoplasmic Membrane
  • Cytoplasmic Score: 0
  • Cytoplasmic Membrane Score: 10
  • Cellwall Score: 0
  • Extracellular Score: 0
  • Internal Helices: 14
• LocateP: Multi-transmembrane
  • Prediction by SwissProt Classification: Membrane
  • Pathway Prediction: Possibly Tat-/Sec-(SPI)
  • Intracellular possibility: 0.17
  • Signal peptide possibility: -0.5
  • N-terminally Anchored Score: -1
  • Predicted Cleavage Site: No CleavageSite
• SignalP: no predicted signal peptide
  • SP(Sec/SPI): 0.330128
  • TAT(Tat/SPI): 0.000713
  • LIPO(Sec/SPII): 0.018141
• predicted transmembrane helices (TMHMM): 14

⊟Accession numbers

• GI: 87159845 NCBI
• RefSeq: YP_492684 NCBI
• UniProt: A0A0H2XKM2 UniProt

⊟Protein sequence

• MFSLYKKFKGLFYSVLFWLCILSFFSVLNEMVLNVSLPDIANHFNTTPGITNWVNTAYMLTFSIGTAVYGKLSDYINIKKLLIIGISLSCLGSLIAFIGHNHFFILIFGRLVQGVGSAAFPSLIMVVVARNITRKKQGKAFGFIGSIVALGEGLGPSIGGIIAHYIHWSYLLILPMITIVTIPFLIKVMVPGKSTKNTLDIVGIVLMSISIICFMLFTTNYNWTFLILFTIFFVIFIKHISRVSNPFINPKLGKNIPFMLGLFSGGLIFSIVAGFISMVPYMMKTIYHVNVATIGNSVIFPGTMSVIVFGYFGGFLVDRKGSLFVFILGSLSISISFLTIAFFVEFSMWLTTFMFIFVMGGLSFTKTVISKIVSSSLSEEEVASGMSLLNFTSFLSEGTGIAIVGGLLSLQLINRKLVLEFINYSSGVYSNILVAMAILIILCCLLTIIVFKRSKKQFE

⊟Experimental data

• experimentally validated:
• protein localization:
• quantitative data / protein copy number per cell:
• interaction partners:

⊟Expression & Regulation

⊟Operon

• MicrobesOnline: no polycistronic organisation predicted

⊟Regulation

• regulator:

⊟Transcription pattern

• S.aureus Expression Data Browser: no data available

⊟Protein synthesis (provided by Aureolib)

• Aureolib: no data available

⊟Protein stability

• half-life: no data available

⊟Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊟Other Information

You are kindly invited to share additional interesting facts.

⊟Literature

⊟References

⊟Relevant publications