COLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 02-MAR-2017

⊟Summary

• organism: Staphylococcus aureus N315
• locus tag: SA_RS00065
• pan locus tag^?:
• symbol: SA_RS00065
• pan gene symbol^?: —
• synonym:
• product: recombinase

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: SA_RS00065
• symbol: SA_RS00065
• product: recombinase
• replicon: pN315
• strand: +
• coordinates: 13378..14046
• length: 669
• essential: unknown

⊟Accession numbers

• Location: NC_003140 (13378..14046) NCBI
• BioCyc: G1G21-2923 BioCyc
• MicrobesOnline:

⊟Phenotype

Share your knowledge and add information here. [edit]

⊟DNA sequence

• 1
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  ATATGTTATGGTCAACCTGGTGTCGGAAAAACTTTATCGTCTAGGTATTACACAAATTGG
  GATACTATTGAAAAACAAGTTAACCATAGAGGTTGGGAAGATTTAGCTAGTAAAACGACC
  GATGACATACTATCGGTGGATAAAATATTTTATACTGCACCAGCAGAAAAACAATCAGGT
  AAATCTATAGAAAACAGACCTGTTTTTCAAGAAGTATTAAATTTTTTAAGAATGGGAGAT
  CGTTTAATTGTTGAATCTATTGATAGACTAGGAAGAAATTACGAGGAAATAATTCAAACA
  GTTAATTACTTAAAAGATAAAGAAGTTCAGCTCATGATAACGAGTTTACCTATGATGAAT
  GAAGTCATTGGAAATCCACTACTAGATAAATTTATGAAAGATTTAATTATTCAAATTTTA
  GCAATGATTTCAGAACAAGAAAGAAATGAAAGTAAACGTCGACAAGCTCAAGGGATTCAA
  GTCGCCAAAGAAAAAGGTATATATAAAGGGAGACCTGTTCTGTATTCTCCCAATGCTAAA
  GACCCACAAAAGCGTTTAGTTTATTACCGAGTTGTTGAATTACTTGAACAGGGTAAATCT
  ATAAGTACTATAGCTAAAGAAGTTGGTATTACACGTCAAACTATATATAGAATAAAAAAT
  AGTAAATAA
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  669
  

⊟Protein

⊟General

• locus tag: SA_RS00065
• symbol: SA_RS00065
• description: recombinase
• length: 222
• theoretical pI: 9.75649
• theoretical MW: 25667.4
• GRAVY: -0.54009

⊟Function

• TIGRFAM:
  Signal transduction Regulatory functions DNA interactions CRISPR locus-related DNA-binding protein (TIGR01884; HMM-score: 16.7)
  Genetic information processing Mobile and extrachromosomal element functions Prophage functions phage-associated protein, BcepMu gp16 family (TIGR04111; HMM-score: 16.7)
  probable regulatory domain (TIGR03879; HMM-score: 15.8)
  RNA polymerase sigma factor, sigma-70 family (TIGR02937; HMM-score: 15.5)
• TheSEED:
• PFAM:
  no clan defined Resolvase; Resolvase, N terminal domain (PF00239; HMM-score: 78.6)
  and 26 more
  HTH (CL0123) HTH_7; Helix-turn-helix domain of resolvase (PF02796; HMM-score: 58.2)
  HTH_23; Homeodomain-like domain (PF13384; HMM-score: 32.2)
  P-loop_NTPase (CL0023) AAA_22; AAA domain (PF13401; HMM-score: 28.4)
  HTH (CL0123) CENP-B_N; CENP-B N-terminal DNA-binding domain (PF04218; HMM-score: 28.2)
  HTH_29; Winged helix-turn helix (PF13551; HMM-score: 25)
  HTH_38; Helix-turn-helix domain (PF13936; HMM-score: 24.3)
  HTH_28; Helix-turn-helix domain (PF13518; HMM-score: 24.2)
  HTH_40; Helix-turn-helix domain (PF14493; HMM-score: 19.6)
  DUF433; Protein of unknown function (DUF433) (PF04255; HMM-score: 19)
  no clan defined DUF2802; Protein of unknown function (DUF2802) (PF10975; HMM-score: 18.7)
  HTH (CL0123) HTH_Tnp_ISL3; Helix-turn-helix domain of transposase family ISL3 (PF13542; HMM-score: 18.5)
  HTH_3; Helix-turn-helix (PF01381; HMM-score: 17.7)
  HTH_11; HTH domain (PF08279; HMM-score: 15.8)
  HTH_22; HTH domain (PF13309; HMM-score: 14.3)
  HTH_36; Helix-turn-helix domain (PF13730; HMM-score: 14.2)
  Trp_repressor; Trp repressor protein (PF01371; HMM-score: 14)
  HTH_Tnp_1_2; Helix-turn-helix of insertion element transposase (PF13022; HMM-score: 14)
  HTH_Tnp_1; Transposase (PF01527; HMM-score: 13.8)
  P-loop_NTPase (CL0023) RuvB_N; Holliday junction DNA helicase ruvB N-terminus (PF05496; HMM-score: 13.7)
  HTH (CL0123) Sigma70_r4; Sigma-70, region 4 (PF04545; HMM-score: 13.4)
  TetR_N; Bacterial regulatory proteins, tetR family (PF00440; HMM-score: 13.1)
  GerE; Bacterial regulatory proteins, luxR family (PF00196; HMM-score: 12.6)
  TrmB; Sugar-specific transcriptional regulator TrmB (PF01978; HMM-score: 12.6)
  no clan defined TnsD; Tn7-like transposition protein D (PF15978; HMM-score: 12.3)
  DUF3572; Protein of unknown function (DUF3572) (PF12096; HMM-score: 12.1)
  HTH (CL0123) Sigma70_r4_2; Sigma-70, region 4 (PF08281; HMM-score: 11.2)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors:
• effectors:

⊟Localization

• PSORTb: Cytoplasmic
  • Cytoplasmic Score: 7.5
  • Cytoplasmic Membrane Score: 1.15
  • Cellwall Score: 0.62
  • Extracellular Score: 0.73
  • Internal Helices: 0
• LocateP:
• SignalP: no predicted signal peptide
  • SP(Sec/SPI): 0.006492
  • TAT(Tat/SPI): 0.000365
  • LIPO(Sec/SPII): 0.000986
• predicted transmembrane helices (TMHMM): 0

⊟Accession numbers

• GI:
• RefSeq: WP_078061227 NCBI
• UniProt:

⊟Protein sequence

• MCYGQPGVGKTLSSRYYTNWDTIEKQVNHRGWEDLASKTTDDILSVDKIFYTAPAEKQSGKSIENRPVFQEVLNFLRMGDRLIVESIDRLGRNYEEIIQTVNYLKDKEVQLMITSLPMMNEVIGNPLLDKFMKDLIIQILAMISEQERNESKRRQAQGIQVAKEKGIYKGRPVLYSPNAKDPQKRLVYYRVVELLEQGKSISTIAKEVGITRQTIYRIKNSK

⊟Experimental data

• experimentally validated:
• protein localization:
• quantitative data / protein copy number per cell:
• interaction partners:

⊟Expression & Regulation

⊟Operon

⊟Regulation

• regulator:

⊟Transcription pattern

• S.aureus Expression Data Browser: no data available

⊟Protein synthesis (provided by Aureolib)

• Aureolib: no data available

⊟Protein stability

• half-life: no data available

⊟Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊟Other Information

You are kindly invited to share additional interesting facts.

⊟Literature

⊟References

⊟Relevant publications