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NCBI: 01-DEC-2025

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus JSNZ
  • locus tag: EGJ38_001940 [new locus tag: EGJ38_RS09685 ]
  • pan locus tag?: SAUPAN004937000
  • symbol: purB
  • pan gene symbol?: purB
  • synonym:
  • alternate name: JSNZ_001940
  • product: adenylosuccinate lyase

⊟Additional information (user-provided)[edit | edit source]

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: EGJ38_001940 [new locus tag: EGJ38_RS09685 ]
  • symbol: purB
  • product: adenylosuccinate lyase
  • replicon: chromosome
  • strand: -
  • coordinates: 1955705..1957000
  • length: 1296
  • essential: unknown other strains

⊟Accession numbers[edit | edit source]

  • Gene ID:
  • RefSeq: MGT2423849 NCBI
  • BioCyc:
  • MicrobesOnline:

⊟Phenotype[edit | edit source]

⊟Additional information (user-provided)[edit | edit source]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    ATGATTGAACGCTATTCTAGAGAAGAAATGTCTAATATTTGGACTGATCAAAATCGCTAT
    GAAGCATGGTTAGAAGTGGAAATTTTAGCATGTGAAGCATGGAGTGAATTAGGACATATA
    CCGAAAGCTGACGTACAAAAAATTCGTCAAAACGCAAAGGTAAATGTCGAACGTGCACAA
    GAAATTGAACAAGAAACGCGCCATGATGTTGTAGCCTTTACTAGACAAGTTTCTGAAACG
    CTAGGTGAAGAACGTAAGTGGGTACATTATGGTTTAACTTCTACTGATGTTGTAGATACA
    GCTTTAAGTTTCGTTATTAAACAAGCAAATGACATTATTGAAAAAGATTTAGAAAGATTT
    ATCGACGTTTTAGCTGAAAAAGCAAAAAATTATAAATATACATTGATGATGGGACGTACA
    CATGGAGTGCATGCAGAACCAACTACATTTGGTGTCAAAATGGCATTGTGGTACACGGAA
    ATGCAACGTAACTTACAACGTTTCAAACAAGTAAGAGAAGAAATTGAAGTAGGGAAAATG
    AGTGGTGCAGTAGGTACTTTTGCTAACATTCCTCCTGAAATAGAAAGTTATGTGTGTAAA
    CATTTAGGTATTGGCACAGCTCCTGTATCAACGCAAACATTGCAACGCGATCGACATGCA
    TACTATATTGCGACATTAGCATTAATCGCGACGTCACTAGAAAAATTTGCCGTTGAAATT
    CGTAACCTTCAAAAAACCGAAACAAGAGAAGTTGAAGAAGCATTTGCAAAAGGCCAAAAA
    GGTTCATCTGCAATGCCTCATAAACGAAATCCAATTGGTTCTGAAAATATCACAGGTATT
    TCAAGAGTCATTCGTGGTTATATTACTACAGCTTATGAGAATGTGCCATTATGGCATGAA
    AGAGATATTTCTCATTCTTCTGCTGAGCGTATTATGTTACCAGATGTTACAATAGCACTA
    GATTATGCATTGAATCGTTTCACAAATATTGTGGACCGTTTAACTGTATTTGAAGATAAT
    ATGCGTAATAACATAGATAAAACATTTGGTCTGATTTTCTCACAACGTGTCTTATTAGCA
    TTAATTAATAAAGGTATGGTTCGTGAAGAAGCATACGACAAAGTACAACCAAAAGCGATG
    ATATCTTGGGAAACGAAAACACCTTTCCGTGAATTAATCGAACAAGATGAGTCTATTACA
    AGTGTTTTAACAAAAGAAGAATTGGATGAATGTTTCGATCCTAAACATCATTTGAATCAA
    GTTGACACTATTTTCGAACGCGCTGGACTAGCATAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1200
    1260
    1296


⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: EGJ38_001940 [new locus tag: EGJ38_RS09685 ]
  • symbol: PurB
  • description: adenylosuccinate lyase
  • length: 431
  • theoretical pI: 5.6693
  • theoretical MW: 49603
  • GRAVY: -0.435963

⊟Function[edit | edit source]

  • reaction:
    EC 4.3.2.2?  ExPASy
    Adenylosuccinate lyase N6-(1,2-dicarboxyethyl)AMP = fumarate + AMP (S)-2-(5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxamido)succinate = fumarate + 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide
  • TIGRFAM:
    Metabolism Purines, pyrimidines, nucleosides, and nucleotides Purine ribonucleotide biosynthesis adenylosuccinate lyase (TIGR00928; EC 4.3.2.2; HMM-score: 581.4)
    and 4 more
    Metabolism Energy metabolism Other 3-carboxy-cis,cis-muconate cycloisomerase (TIGR02426; EC 5.5.1.2; HMM-score: 196)
    Metabolism Amino acid biosynthesis Glutamate family argininosuccinate lyase (TIGR00838; EC 4.3.2.1; HMM-score: 85.6)
    Metabolism Energy metabolism Amino acids and amines aspartate ammonia-lyase (TIGR00839; EC 4.3.1.1; HMM-score: 62.6)
    Metabolism Energy metabolism TCA cycle fumarate hydratase, class II (TIGR00979; EC 4.2.1.2; HMM-score: 56.7)
  • TheSEED: data available for COL, N315, NCTC8325, Newman, USA300_FPR3757
  • PFAM:
    no clan defined Lyase_1; Lyase (PF00206; HMM-score: 261.9)
    and 4 more
    ADSL_C; Adenylosuccinate lyase C-terminus (PF10397; HMM-score: 96.6)
    ASL_C; Adenylosuccinate lyase C-terminal (PF08328; HMM-score: 17.2)
    DUF7337; Family of unknown function (DUF7337) (PF24026; HMM-score: 14.2)
    UPF0047; Uncharacterised protein family UPF0047 (PF01894; HMM-score: 13.6)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 9.97
    • Cytoplasmic Membrane Score: 0
    • Cellwall Score: 0.01
    • Extracellular Score: 0.02
    • Internal Helices: 0
  • DeepLocPro: Cytoplasmic
    • Cytoplasmic Score: 0.9512
    • Cytoplasmic Membrane Score: 0.005
    • Cell wall & surface Score: 0.0003
    • Extracellular Score: 0.0435
  • LocateP:
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.005698
    • TAT(Tat/SPI): 0.00084
    • LIPO(Sec/SPII): 0.009881
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

  • GI:
  • RefSeq: MGT2423849 NCBI
  • UniProt:

⊟Additional information (user-provided)[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MIERYSREEMSNIWTDQNRYEAWLEVEILACEAWSELGHIPKADVQKIRQNAKVNVERAQEIEQETRHDVVAFTRQVSETLGEERKWVHYGLTSTDVVDTALSFVIKQANDIIEKDLERFIDVLAEKAKNYKYTLMMGRTHGVHAEPTTFGVKMALWYTEMQRNLQRFKQVREEIEVGKMSGAVGTFANIPPEIESYVCKHLGIGTAPVSTQTLQRDRHAYYIATLALIATSLEKFAVEIRNLQKTETREVEEAFAKGQKGSSAMPHKRNPIGSENITGISRVIRGYITTAYENVPLWHERDISHSSAERIMLPDVTIALDYALNRFTNIVDRLTVFEDNMRNNIDKTFGLIFSQRVLLALINKGMVREEAYDKVQPKAMISWETKTPFRELIEQDESITSVLTKEELDECFDPKHHLNQVDTIFERAGLA

⊟Experimental data[edit | edit source]

  • experimentally validated: data available for COL, NCTC8325
  • protein localization: data available for COL
  • quantitative data / protein copy number per cell: data available for COL
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator: PurR (repression) regulon
    PurR(TF)important in Purine metabolism;  regulation predicted or transferred from N315 and NCTC 8325  [1]

⊟Additional information (user-provided)[edit | edit source]

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Additional information (user-provided)[edit | edit source]

⊟Other information (user-provided)[edit | edit source]

You can add further information about the gene and protein here. [edit]

⊟Literature[edit | edit source]

⊟References[edit | edit source]

  1. ↑ Hannes Wolfgramm, Larissa Milena Busch, Jöran Tebben, Henry Mehlan, Lisa Hagenau, Thomas Sura, Tilly Hoffmüller, Elisa Bludau, Manuela Gesell Salazar, Alexander Reder, Stephan Michalik, Leif Steil, Kristin Surmann, Ulrike Mäder, Silva Holtfreter, Uwe Völker
    Integrated genomic and proteomic analysis of the mouse-adapted Staphylococcus aureus strain JSNZ.
    Curr Res Microb Sci: 2025, 9;100489
    [PubMed:41146725] [WorldCat.org] [DOI] (I e)

⊟Relevant publications[edit | edit source]

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