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NCBI: 10-JUN-2013

Summary[edit | edit source]

  • organism: Staphylococcus aureus USA300_FPR3757
  • locus tag: SAUSA300_1889 [new locus tag: SAUSA300_RS10335 ]
  • pan locus tag?: SAUPAN004937000
  • symbol: purB
  • pan gene symbol?: purB
  • synonym:
  • product: adenylosuccinate lyase

Genome View[edit | edit source]

Gene[edit | edit source]

General[edit | edit source]

  • type: CDS
  • locus tag: SAUSA300_1889 [new locus tag: SAUSA300_RS10335 ]
  • symbol: purB
  • product: adenylosuccinate lyase
  • replicon: chromosome
  • strand: -
  • coordinates: 2052926..2054221
  • length: 1296
  • essential: unknown other strains

Accession numbers[edit | edit source]

Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    ATGATTGAACGCTATTCTAGAGAAGAAATGTCTAATATTTGGACTGATCAAAATCGCTAT
    GAAGCATGGTTAGAAGTGGAAATTTTAGCATGTGAAGCATGGAGTGAATTAGGACATATA
    CCGAAAGCTGACGTACAAAAAATTCGTCAAAACGCAAAGGTAAATGTCGAACGTGCACAA
    GAAATTGAACAAGAAACGCGCCATGATGTTGTAGCCTTTACTAGACAAGTTTCTGAAACG
    CTAGGTGAAGAACGTAAGTGGGTACATTATGGTTTAACTTCTACTGATGTTGTAGATACA
    GCTTTAAGTTTCGTTATTAAACAAGCAAATGACATTATTGAAAAAGATTTAGAAAGATTT
    ATCGACGTTTTAGCTGAAAAAGCAAAAAATTATAAATATACATTGATGATGGGACGTACA
    CATGGAGTGCATGCAGAACCAACTACATTTGGTGTCAAAATGGCATTGTGGTACACGGAA
    ATGCAACGTAACTTACAACGTTTCAAACAAGTAAGAGAAGAAATTGAAGTAGGGAAAATG
    AGTGGTGCAGTAGGTACTTTTGCTAACATTCCTCCTGAAATAGAAAGTTATGTGTGTAAA
    CATTTAGGTATTGGCACAGCGCCTGTATCAACGCAAACATTGCAACGCGATCGACATGCA
    TACTATATTGCGACATTAGCATTAATCGCGACATCACTAGAAAAATTTGCCGTTGAAATA
    CGTAACCTTCAAAAAACCGAAACGAGAGAAGTTGAAGAAGCATTTGCAAAAGGCCAAAAA
    GGTTCATCTGCAATGCCTCATAAACGAAATCCAATTGGTTCTGAAAATATCACAGGTATT
    TCAAGAGTCATTCGTGGTTATATTACTACAGCTTATGAGAATGTGCCATTATGGCATGAA
    AGAGATATTTCTCATTCTTCTGCTGAGCGTATTATGTTACCAGATGTTACAATAGCACTA
    GATTATGCATTGAATCGTTTCACAAATATTGTGGACCGTTTAACTGTATTTGAAGATAAT
    ATGCGTAATAACATAGATAAAACATTTGGTCTGATTTTCTCACAACGTGTCTTATTAGCA
    TTAATTAATAAAGGTATGGTTCGTGAAGAAGCATACGACAAAGTACAACCAAAAGCGATG
    ATATCTTGGGAAACGAAAACACCGTTCCGTGAATTAATCGAACAAGATGAGTCTATTACA
    AGTGTTTTAACAAAAGAAGAATTGGATGAATGTTTCGATCCTAAACATCATTTGAATCAA
    GTTGACACTATTTTCGAACGCGCTGGATTAGCATAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1200
    1260
    1296

Protein[edit | edit source]

General[edit | edit source]

  • locus tag: SAUSA300_1889 [new locus tag: SAUSA300_RS10335 ]
  • symbol: PurB
  • description: adenylosuccinate lyase
  • length: 431
  • theoretical pI: 5.6693
  • theoretical MW: 49603
  • GRAVY: -0.435963

Function[edit | edit source]

  • reaction:
    EC 4.3.2.2?  ExPASy
    Adenylosuccinate lyase N6-(1,2-dicarboxyethyl)AMP = fumarate + AMP (S)-2-(5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxamido)succinate = fumarate + 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide
  • TIGRFAM:
    Metabolism Purines, pyrimidines, nucleosides, and nucleotides Purine ribonucleotide biosynthesis adenylosuccinate lyase (TIGR00928; EC 4.3.2.2; HMM-score: 581.4)
    and 4 more
    Metabolism Energy metabolism Other 3-carboxy-cis,cis-muconate cycloisomerase (TIGR02426; EC 5.5.1.2; HMM-score: 196)
    Metabolism Amino acid biosynthesis Glutamate family argininosuccinate lyase (TIGR00838; EC 4.3.2.1; HMM-score: 85.6)
    Metabolism Energy metabolism Amino acids and amines aspartate ammonia-lyase (TIGR00839; EC 4.3.1.1; HMM-score: 62.6)
    Metabolism Energy metabolism TCA cycle fumarate hydratase, class II (TIGR00979; EC 4.2.1.2; HMM-score: 56.7)
  • TheSEED:  
    Nucleosides and Nucleotides Purines De Novo Purine Biosynthesis  Adenylosuccinate lyase (EC 4.3.2.2)
    and 1 more
    Purine conversions  Adenylosuccinate lyase (EC 4.3.2.2)
  • PFAM:
    no clan defined Lyase_1; Lyase (PF00206; HMM-score: 253.4)
    and 2 more
    ADSL_C; Adenylosuccinate lyase C-terminus (PF10397; HMM-score: 92.5)
    ASL_C; Adenylosuccinate lyase C-terminal (PF08328; HMM-score: 15.7)

Structure, modifications & interactions[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:
  • protein partners:

Localization[edit | edit source]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 9.97
    • Cytoplasmic Membrane Score: 0
    • Cellwall Score: 0.01
    • Extracellular Score: 0.02
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular possibility: 1
    • Signal peptide possibility: -1
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.005698
    • TAT(Tat/SPI): 0.00084
    • LIPO(Sec/SPII): 0.009881
  • predicted transmembrane helices (TMHMM): 0

Accession numbers[edit | edit source]

Protein sequence[edit | edit source]

  • MIERYSREEMSNIWTDQNRYEAWLEVEILACEAWSELGHIPKADVQKIRQNAKVNVERAQEIEQETRHDVVAFTRQVSETLGEERKWVHYGLTSTDVVDTALSFVIKQANDIIEKDLERFIDVLAEKAKNYKYTLMMGRTHGVHAEPTTFGVKMALWYTEMQRNLQRFKQVREEIEVGKMSGAVGTFANIPPEIESYVCKHLGIGTAPVSTQTLQRDRHAYYIATLALIATSLEKFAVEIRNLQKTETREVEEAFAKGQKGSSAMPHKRNPIGSENITGISRVIRGYITTAYENVPLWHERDISHSSAERIMLPDVTIALDYALNRFTNIVDRLTVFEDNMRNNIDKTFGLIFSQRVLLALINKGMVREEAYDKVQPKAMISWETKTPFRELIEQDESITSVLTKEELDECFDPKHHLNQVDTIFERAGLA

Experimental data[edit | edit source]

  • experimentally validated: no data available

Expression & Regulation[edit | edit source]

Operon[edit | edit source]

  • purB no polycistronic organisation predicted

Regulation[edit | edit source]

  • regulator:

Transcription pattern[edit | edit source]

Protein synthesis (provided by Aureolib)[edit | edit source]

Protein stability[edit | edit source]

  • half-life: no data available

Biological Material[edit | edit source]

Mutants[edit | edit source]

Expression vector[edit | edit source]

lacZ fusion[edit | edit source]

GFP fusion[edit | edit source]

two-hybrid system[edit | edit source]

FLAG-tag construct[edit | edit source]

Antibody[edit | edit source]

Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

Literature[edit | edit source]

References[edit | edit source]

Relevant publications[edit | edit source]