PangenomeCOLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 02-MAR-2017

⊟Summary

• organism: Staphylococcus aureus USA300_FPR3757
• locus tag: SAUSA300_RS10335 [old locus tag: SAUSA300_1889 ]
• pan locus tag^?: SAUPAN004937000
• symbol: SAUSA300_RS10335
• pan gene symbol^?: purB
• synonym:
• product: adenylosuccinate lyase

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: SAUSA300_RS10335 [old locus tag: SAUSA300_1889 ]
• symbol: SAUSA300_RS10335
• product: adenylosuccinate lyase
• replicon: chromosome
• strand: -
• coordinates: 2052926..2054221
• length: 1296
• essential: unknown other strains

⊟Accession numbers

• Location: NC_007793 (2052926..2054221) NCBI
• BioCyc: SAUSA300_RS10335 BioCyc
• MicrobesOnline: see SAUSA300_1889

⊟Phenotype

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⊟DNA sequence

• 1
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  ATGATTGAACGCTATTCTAGAGAAGAAATGTCTAATATTTGGACTGATCAAAATCGCTAT
  GAAGCATGGTTAGAAGTGGAAATTTTAGCATGTGAAGCATGGAGTGAATTAGGACATATA
  CCGAAAGCTGACGTACAAAAAATTCGTCAAAACGCAAAGGTAAATGTCGAACGTGCACAA
  GAAATTGAACAAGAAACGCGCCATGATGTTGTAGCCTTTACTAGACAAGTTTCTGAAACG
  CTAGGTGAAGAACGTAAGTGGGTACATTATGGTTTAACTTCTACTGATGTTGTAGATACA
  GCTTTAAGTTTCGTTATTAAACAAGCAAATGACATTATTGAAAAAGATTTAGAAAGATTT
  ATCGACGTTTTAGCTGAAAAAGCAAAAAATTATAAATATACATTGATGATGGGACGTACA
  CATGGAGTGCATGCAGAACCAACTACATTTGGTGTCAAAATGGCATTGTGGTACACGGAA
  ATGCAACGTAACTTACAACGTTTCAAACAAGTAAGAGAAGAAATTGAAGTAGGGAAAATG
  AGTGGTGCAGTAGGTACTTTTGCTAACATTCCTCCTGAAATAGAAAGTTATGTGTGTAAA
  CATTTAGGTATTGGCACAGCGCCTGTATCAACGCAAACATTGCAACGCGATCGACATGCA
  TACTATATTGCGACATTAGCATTAATCGCGACATCACTAGAAAAATTTGCCGTTGAAATA
  CGTAACCTTCAAAAAACCGAAACGAGAGAAGTTGAAGAAGCATTTGCAAAAGGCCAAAAA
  GGTTCATCTGCAATGCCTCATAAACGAAATCCAATTGGTTCTGAAAATATCACAGGTATT
  TCAAGAGTCATTCGTGGTTATATTACTACAGCTTATGAGAATGTGCCATTATGGCATGAA
  AGAGATATTTCTCATTCTTCTGCTGAGCGTATTATGTTACCAGATGTTACAATAGCACTA
  GATTATGCATTGAATCGTTTCACAAATATTGTGGACCGTTTAACTGTATTTGAAGATAAT
  ATGCGTAATAACATAGATAAAACATTTGGTCTGATTTTCTCACAACGTGTCTTATTAGCA
  TTAATTAATAAAGGTATGGTTCGTGAAGAAGCATACGACAAAGTACAACCAAAAGCGATG
  ATATCTTGGGAAACGAAAACACCGTTCCGTGAATTAATCGAACAAGATGAGTCTATTACA
  AGTGTTTTAACAAAAGAAGAATTGGATGAATGTTTCGATCCTAAACATCATTTGAATCAA
  GTTGACACTATTTTCGAACGCGCTGGATTAGCATAA
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⊟Protein

⊟General

• locus tag: SAUSA300_RS10335 [old locus tag: SAUSA300_1889 ]
• symbol: SAUSA300_RS10335
• description: adenylosuccinate lyase
• length: 431
• theoretical pI: 5.6693
• theoretical MW: 49603
• GRAVY: -0.435963

⊟Function

• reaction:
  EC 4.3.2.2?  ExPASy
  Adenylosuccinate lyase N⁶-(1,2-dicarboxyethyl)AMP = fumarate + AMP (S)-2-(5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxamido)succinate = fumarate + 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide
• TIGRFAM:
  Metabolism Purines, pyrimidines, nucleosides, and nucleotides Purine ribonucleotide biosynthesis adenylosuccinate lyase (TIGR00928; EC 4.3.2.2; HMM-score: 581.4)
  and 4 more
  Metabolism Energy metabolism Other 3-carboxy-cis,cis-muconate cycloisomerase (TIGR02426; EC 5.5.1.2; HMM-score: 196)
  Metabolism Amino acid biosynthesis Glutamate family argininosuccinate lyase (TIGR00838; EC 4.3.2.1; HMM-score: 85.6)
  Metabolism Energy metabolism Amino acids and amines aspartate ammonia-lyase (TIGR00839; EC 4.3.1.1; HMM-score: 62.6)
  Metabolism Energy metabolism TCA cycle fumarate hydratase, class II (TIGR00979; EC 4.2.1.2; HMM-score: 56.7)
• TheSEED: see SAUSA300_1889
• PFAM:
  no clan defined Lyase_1; Lyase (PF00206; HMM-score: 253.4)
  and 2 more
  ADSL_C; Adenylosuccinate lyase C-terminus (PF10397; HMM-score: 92.5)
  ASL_C; Adenylosuccinate lyase C-terminal (PF08328; HMM-score: 15.7)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors:
• effectors:

⊟Localization

• PSORTb: Cytoplasmic
  • Cytoplasmic Score: 9.97
  • Cytoplasmic Membrane Score: 0
  • Cellwall Score: 0.01
  • Extracellular Score: 0.02
  • Internal Helices: 0
• LocateP:
• SignalP: no predicted signal peptide
  • SP(Sec/SPI): 0.005698
  • TAT(Tat/SPI): 0.00084
  • LIPO(Sec/SPII): 0.009881
• predicted transmembrane helices (TMHMM): 0

⊟Accession numbers

• GI: 446495024 NCBI
• RefSeq: WP_000572878 NCBI
• UniProt: see SAUSA300_1889

⊟Protein sequence

• MIERYSREEMSNIWTDQNRYEAWLEVEILACEAWSELGHIPKADVQKIRQNAKVNVERAQEIEQETRHDVVAFTRQVSETLGEERKWVHYGLTSTDVVDTALSFVIKQANDIIEKDLERFIDVLAEKAKNYKYTLMMGRTHGVHAEPTTFGVKMALWYTEMQRNLQRFKQVREEIEVGKMSGAVGTFANIPPEIESYVCKHLGIGTAPVSTQTLQRDRHAYYIATLALIATSLEKFAVEIRNLQKTETREVEEAFAKGQKGSSAMPHKRNPIGSENITGISRVIRGYITTAYENVPLWHERDISHSSAERIMLPDVTIALDYALNRFTNIVDRLTVFEDNMRNNIDKTFGLIFSQRVLLALINKGMVREEAYDKVQPKAMISWETKTPFRELIEQDESITSVLTKEELDECFDPKHHLNQVDTIFERAGLA

⊟Experimental data

• experimentally validated: data available for COL, NCTC8325
• protein localization: data available for COL
• quantitative data / protein copy number per cell: data available for COL
• interaction partners:

⊟Expression & Regulation

⊟Operon

⊟Regulation

• data available for N315

⊟Transcription pattern

• S.aureus Expression Data Browser: data available for NCTC8325

⊟Protein synthesis (provided by Aureolib)

• Aureolib: data available for COL

⊟Protein stability

• half-life: no data available

⊟Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊟Other Information

You are kindly invited to share additional interesting facts.

⊟Literature

⊟References

⊟Relevant publications