PangenomeCOLN315NCTC8325NewmanUSA300_FPR3757JSNZ04-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

FunGene: 08-OCT-2024

⊟Summary

• organism: Staphylococcus aureus JSNZ
• locus tag: JSNZ_000025
• pan locus tag^?: SAUPAN000037000
• symbol: adsA
• pan gene symbol^?: adsA
• synonym:
• product: LPXTG-anchored adenosine synthase AdsA

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: JSNZ_000025
• symbol: adsA
• product: LPXTG-anchored adenosine synthase AdsA
• replicon: chromosome
• strand: +
• coordinates: 30476..32794
• length: 2319
• essential: unknown other strains

⊟Accession numbers

• Gene ID:
• RefSeq:
• BioCyc:
• MicrobesOnline:

⊟Phenotype

Share your knowledge and add information here. [edit]

⊟DNA sequence

• 1
  61
  121
  181
  241
  301
  361
  421
  481
  541
  601
  661
  721
  781
  841
  901
  961
  1021
  1081
  1141
  1201
  1261
  1321
  1381
  1441
  1501
  1561
  1621
  1681
  1741
  1801
  1861
  1921
  1981
  2041
  2101
  2161
  2221
  2281
  
  ATGAAAGCTTTATTACTTAAAACAAGTGTATGGCTCGTTTTGCTTTTTAGTGTGATGGGA
  TTATGGCATGTCTCGAACGCGGCTGAGCAACATACACCAATGAAAGCACATGCAGTAACA
  ACGATAGACCAAGCAGCAACAGATAAGCAATTAGTACCACCAACCAAGGAAGCAGCTCAT
  CAATTTGGTGAAGAAGCGGCAACCAACGTATCAGCATCAGCACAGGGAACAGCTGATGAA
  ATAAACAATAAAGTAACATCCAACGCATTTTCTAACAAACCATCTACAGCAGTTTCAACA
  AAAGTAAACGAAACGCACGATGTAGATACACAACAAGCCTCAACACAAAAACCAACTCAA
  TCAGCAACATTCACATTATCAAATGCTAAAACAGCATCACTTTCACCACGAATGTTTGCT
  GCCAATGTACCACAAACAACAACACATAAAATATTACATACAAATGATATCCATGGCCGA
  CTAGCCGAAGAAAAAGGGCGTGTCATCGGTATGGCTAAATTAAAAACAGTAAAAGAACAA
  GAAAAGCCTGATTTAATGTTAGACGCAGGAGACGCCTTCCAAGGTTTACCGCTTTCAAAC
  CAGTCTAAAGGTGAAGAAATGGCTAAAGCAATGAATGCAGTAGGTTATGATGCTATGGCA
  GTCGGTAACCACGAATTTGACTTTGGATACGACCAGTTGAAAAAATTAGAGGGTATGTTA
  GACTTCCCGATGCTAAGTACTAACGTTTACAAAGATGGGAAACGCGCGTTTAAGCCTTCA
  ACGATTGTAACGAAAAATGGTATTCGTTATGGAATTATTGGCGTAACGACACCAGAAACA
  AAGACGAAAACAAGACCTGAAGGCATTAAAGGTGTTGAATTTAGAGATCCATTACAAAGT
  GTGACAGCAGAAATGATGCGTATTTATAAAGACGTAGATACATTTGTTGTTATATCACAT
  TTAGGGATTGATCCTTCAACGCAAGAAACATGGCGTGGTGATTACTTAGTGAAACAATTA
  AGTCAAAATCCACAATTGAAGAAACGTATTACAGTTATTGATGGTCATTCACATACCGTA
  CTTCAAAATGGTCAAATTTATAACAATGATGCATTGGCACAAACAGGTACAGCACTTGCG
  AATATCGGTAAGATTACATTTAATTACCGCAATGGAGAGGTATCGAATATTAAGCCGTCA
  TTGATTAATGTTAAAGACGTTGAAAATGTGACACCGAACAAAGCATTAGCTGAACAAATT
  AATCAAGCTGATCAAACATTTAGAGCACAAACAGCAGAGGTTATTATTCCAAATAATACG
  ATTGATTTCAAAGGAGAAAGAGATGACGTTAGAACGCGTGAAACAAATTTAGGAAACGCG
  ATTGCAGATGCTATGGAAGCATATGGCGTTAAGAATTTCTCTAAAAAGACTGACTTCGCC
  GTGACAAATGGTGGAGGTATTCGTGCCTCTATCGCAAAAGGTAAGGTGACACGCTATGAT
  TTAATCTCAGTATTACCATTTGGAAATACGATTGCGCAAATTGATGTAAAAGGTTCAGAC
  GTCTGGACGGCTTTCGAACATAGTTTAGGTGCACCAACAACACAAAAAGACGGTAAGACA
  GTGTTAACAGCGAATGGCGGTTTATTACATATCTCTGATTCAATTCGTGTTTACTATGAT
  ATGAATAAACCGTCTGGCAAACGAATTAATGCTATTCAAATTTTAAATAAAGAGACAGGT
  AAGTTTGAAAGTATTGATTTAAAACGTGTATATCATGTAACGATGAATGACTTCACAGCA
  TCAGGTGGAGACGGATATAGTATGTTCGGTGGCCCTAGAGAAGAAGGTATTTCATTAGAT
  CAAGTACTAGCAAGTTATTTAAAAACAGCTAACTTAGCTAAGTATGATATGACAGAACCA
  CAACGTATGTTATTAGGTAAACCAGTAGCAAGTGAACAACCAGCTAAAGGACAACAAGGT
  AGCAAAGGTAGTGAGTCTGGTAAAGATACACAACCAATTGGTAAAGAGAAAGTGATGAAT
  CCAGCGAAACAACCAGCGACAGGTAAAGTTGTGTTGTTACCAGCGCATAGAGGAACTGTT
  AGTAGCGGTACAGAAGGTTCTGATCGCGCATTAGAAGGAACTGCTGTATCAAGTAAGAGT
  GGGAAACAATTGGCTAACATGTCAGCGCCTAAAGGTAGCGCACATGAGAAACAGTTACCA
  AAAACTGGAACTGATCAAAGTTCAAGCCCAGCAGCGATGTTTGTATTAGTAACAGGTATA
  GGTTTAATCGCGACTGTACGACGTAGAAAAGCTAGCTAA
  60
  120
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  420
  480
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  720
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  1140
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  1260
  1320
  1380
  1440
  1500
  1560
  1620
  1680
  1740
  1800
  1860
  1920
  1980
  2040
  2100
  2160
  2220
  2280
  2319
  

⊟Protein

⊟General

• locus tag: JSNZ_000025
• symbol: AdsA
• description: LPXTG-anchored adenosine synthase AdsA
• length: 772
• theoretical pI: 9.78853
• theoretical MW: 83426.9
• GRAVY: -0.449482

⊟Function

• TIGRFAM:
  Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Pyridine nucleotides NAD nucleotidase (TIGR01530; EC 3.1.3.5,3.6.1.22; HMM-score: 135.2)
  Metabolism Transport and binding proteins Other NAD nucleotidase (TIGR01530; EC 3.1.3.5,3.6.1.22; HMM-score: 135.2)
  and 4 more
  thiosulfohydrolase SoxB (TIGR04486; HMM-score: 91.9)
  Metabolism Purines, pyrimidines, nucleosides, and nucleotides Other 2',3'-cyclic-nucleotide 2'-phosphodiesterase (TIGR01390; EC 3.1.4.16; HMM-score: 52.8)
  Cell structure Cell envelope Other LPXTG cell wall anchor domain (TIGR01167; HMM-score: 23.7)
  Genetic information processing DNA metabolism DNA replication, recombination, and repair exonuclease SbcCD, D subunit (TIGR00619; HMM-score: 13.2)
• TheSEED: data available for COL, N315, NCTC8325, Newman, USA300_FPR3757
• PFAM:
  NadN_C (CL0781) 5_nucleotid_C; 5'-nucleotidase, C-terminal domain (PF02872; HMM-score: 130.5)
  and 3 more
  Calcineurin (CL0163) Metallophos; Calcineurin-like phosphoesterase (PF00149; HMM-score: 46.5)
  NadN_C (CL0781) NadN_nucleosid_C; NAD nucleotidase, C-terminal domain (PF21953; HMM-score: 18.7)
  no clan defined Gram_pos_anchor; LPXTG cell wall anchor motif (PF00746; HMM-score: 13.9)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors:
• effectors:

⊟Localization

• PSORTb: Cellwall
  • Cytoplasmic Score: 0
  • Cytoplasmic Membrane Score: 0
  • Cellwall Score: 10
  • Extracellular Score: 0
  • Internal Helices: 2
• DeepLocPro: Cell wall & surface
  • Cytoplasmic Score: 0.0008
  • Cytoplasmic Membrane Score: 0.1189
  • Cell wall & surface Score: 0.716
  • Extracellular Score: 0.1643
• LocateP:
• SignalP: Signal peptide SP(Sec/SPI) length 27 aa
  • SP(Sec/SPI): 0.989893
  • TAT(Tat/SPI): 0.001247
  • LIPO(Sec/SPII): 0.003835
  • Cleavage Site: CS pos: 27-28. SNA-AE. Pr: 0.8846
• predicted transmembrane helices (TMHMM): 0

⊟Accession numbers

• GI:
• RefSeq:
• UniProt:

⊟Protein sequence

• MKALLLKTSVWLVLLFSVMGLWHVSNAAEQHTPMKAHAVTTIDQAATDKQLVPPTKEAAHQFGEEAATNVSASAQGTADEINNKVTSNAFSNKPSTAVSTKVNETHDVDTQQASTQKPTQSATFTLSNAKTASLSPRMFAANVPQTTTHKILHTNDIHGRLAEEKGRVIGMAKLKTVKEQEKPDLMLDAGDAFQGLPLSNQSKGEEMAKAMNAVGYDAMAVGNHEFDFGYDQLKKLEGMLDFPMLSTNVYKDGKRAFKPSTIVTKNGIRYGIIGVTTPETKTKTRPEGIKGVEFRDPLQSVTAEMMRIYKDVDTFVVISHLGIDPSTQETWRGDYLVKQLSQNPQLKKRITVIDGHSHTVLQNGQIYNNDALAQTGTALANIGKITFNYRNGEVSNIKPSLINVKDVENVTPNKALAEQINQADQTFRAQTAEVIIPNNTIDFKGERDDVRTRETNLGNAIADAMEAYGVKNFSKKTDFAVTNGGGIRASIAKGKVTRYDLISVLPFGNTIAQIDVKGSDVWTAFEHSLGAPTTQKDGKTVLTANGGLLHISDSIRVYYDMNKPSGKRINAIQILNKETGKFESIDLKRVYHVTMNDFTASGGDGYSMFGGPREEGISLDQVLASYLKTANLAKYDMTEPQRMLLGKPVASEQPAKGQQGSKGSESGKDTQPIGKEKVMNPAKQPATGKVVLLPAHRGTVSSGTEGSDRALEGTAVSSKSGKQLANMSAPKGSAHEKQLPKTGTDQSSSPAAMFVLVTGIGLIATVRRRKAS

⊟Experimental data

• experimentally validated: data available for COL, NCTC8325
• protein localization: data available for COL
• quantitative data / protein copy number per cell:
• interaction partners:

⊟Expression & Regulation

⊟Operon

⊟Regulation

• regulator:

⊟Transcription pattern

• S.aureus Expression Data Browser: data available for NCTC8325

⊟Protein synthesis (provided by Aureolib)

• Aureolib: no data available

⊟Protein stability

• half-life: no data available

⊟Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊟Other Information

You can add further information about the gene and protein here. [edit]

⊟Literature

⊟References

⊟Relevant publications

Vilasack Thammavongsa, Justin W Kern, Dominique M Missiakas, Olaf Schneewind
Staphylococcus aureus synthesizes adenosine to escape host immune responses.
J Exp Med: 2009, 206(11);2417-27
[PubMed:19808256] [WorldCat.org] [DOI] (I p)Vilasack Thammavongsa, Olaf Schneewind, Dominique M Missiakas
Enzymatic properties of Staphylococcus aureus adenosine synthase (AdsA).
BMC Biochem: 2011, 12;56
[PubMed:22035583] [WorldCat.org] [DOI] (I e)Hwan Keun Kim, Vilasack Thammavongsa, Olaf Schneewind, Dominique Missiakas
Recurrent infections and immune evasion strategies of Staphylococcus aureus.
Curr Opin Microbiol: 2012, 15(1);92-9
[PubMed:22088393] [WorldCat.org] [DOI] (I p)