⊟Summary[edit | edit source]
- organism: Staphylococcus aureus N315
- locus tag: SA0022 [new locus tag: SA_RS00265 ]
- pan locus tag?: SAUPAN000037000
- symbol: SA0022
- pan gene symbol?: adsA
- synonym:
- product: hypothetical protein
⊟Genome View[edit | edit source]
⊟Gene[edit | edit source]
⊟General[edit | edit source]
- type: CDS
- locus tag: SA0022 [new locus tag: SA_RS00265 ]
- symbol: SA0022
- product: hypothetical protein
- replicon: chromosome
- strand: +
- coordinates: 31006..33324
- length: 2319
- essential: no DEG other strains
⊟Accession numbers[edit | edit source]
- Gene ID: 1122796 NCBI
- RefSeq: NP_373261 NCBI
- BioCyc: see SA_RS00265
- MicrobesOnline: 102287 MicrobesOnline
⊟Phenotype[edit | edit source]
Share your knowledge and add information here. [edit]
⊟DNA sequence[edit | edit source]
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2281ATGAAAGCTTTATTACTTAAAACAAGTGTATGGCTCGTTTTGCTTTTTAGTGTGATGGGA
TTATGGCAAGTCTCGAACGCGGCTGAGCAGTATACACCAATCAAAGCACATGTAGTAACA
ACGATAGACAAAGCAACAACAGATAAGCAACAAGTAACGCCAACAAAGGAAGCGGCTCAT
CAATTTGGTGAAGAAGCGGCAACCAACGTATCAGCATCAGCACAGGGAACAGCTGATGAA
ATAAACAATAAAGTAACATCCAACGCATTTTCTAACAAACCATCTACAGCAGTTTCAACA
AAAGTAAACGAAACGCACGATGTAGATACACAACAAGCCTCAACACAAAAACCAACTCAA
TCAGCAACATTCACATTATCAAATGCTAAAACAGCATCACTTTCACCACGAATGTTTGCT
GCCAATGTACCACAAACAACAACACATAAAATATTACATACAAATGATATCCATGGCCGA
CTAGCCGAAGAAAAAGGGCGTGTCATCGGTATGGCTAAATTAAAAACAATAAAAGAACAA
GAAAAGCCTGATTTAATGTTAGACGCAGGAGACGCCTTCCAAGGTTTACCACTTTCAAAC
CAGTCTAAAGGTGAAGAAATGGCTAAAGCAATGAATGCAGTAGGTTATGATGCTATGGCA
GTGGGTAACCATGAATTTGACTTTGGATACGATCAGTTGAAAAAGTTAGAGGGTATGTTA
GACTTCCCGATGCTAAGTACTAACGTTTACAAAGATGGGAAACGCGCGTTTAAGCCTTCA
ACAATTGTAACGAAAAATGGTATTCGTTATGGAATTATTGGCGTAACGACACCAGAAACA
AAGACGAAAACAAGACCTGAGGGCATTAAAGGTGTTGAATTTAGAGATCCATTACAAAGT
GTGACAGCAGAAATGATGCGTATTTATAAAGACGTAGATACATTTGTTGTTATATCACAT
TTAGGGATTGATCCTTCAACACAAGAAACATGGCGTGGTGATTACTTAGTGAAACAATTA
AGTCAAAATCCACAATTGAAGAAACGTATTACAGTCATTGATGGTCATTCACATACCGTA
CTTCAAAATGGTCAAATTTATAACAATGATGCATTAGCACAAACAGGTACAGCACTTGCG
AATATCGGTAAGGTTACATTTAATTACCGCAATGGAGAGGTATCAAATATTAAACCGTCA
TTGATTAATGTTAAAGACGTTGAAAATGTAACACCGAACAAAGCATTAGCTGAACAAATT
AATCAAGCTGATCAAACATTTAGAGCACAAACAGCAGAGGTTATTATTCCAAATAATACC
ATTGATTTCAAAGGAGAAAGAGATGACGTTAGAACGCGTGAAACAAATTTAGGAAACGCG
ATTGCAGATGCTATGGAAGCGTATGGCGTTAAGAATTTCTCTAAAAAGACTGACTTTGCC
GTGACAAATGGTGGAGGTATTCGTGCCTCTATCGCAAAAGGTAAGGTGACACGCTATGAT
TTAATCTCAGTATTACCATTTGGAAATACGATTGCGCAAATTGATGTAAAAGGTTCAGAC
GTCTGGACAGCTTTCGAACATAGTTTAGGTGCACCAACAACACAAAAAGACGGTAAGACA
GTATTAACAGCGAATGGCGGTTTACTACATATCTCTGATTCAATTCGTGTTTACTATGAT
ATGAATAAACCGTCTGGCAAACGAATTAACGCTATTCAAATTTTAAATAAAGAGACAGGT
AAGTTTGAAAATATTGATTTAAAACGTGTATATCATGTAACGATGAATGACTTCACAGCA
TCAGGTGGCGACGGATATAGTATGTTCGGTGGCCCTAGAGAAGAAGGTATTTCATTAGAT
CAAGTACTAGCAAGTTATTTAAAAACAGCTAACATAGCTAAGTATGATACGACAGAACCA
CAACGTATGTTATTAGGTAAACCAGCAGTAAGTGAACAACCAGCTAAAGGACAACAAGGT
AGCAAAGGTAGTGAGTCTGGTAAAGATGTACAACCAATTGGTGACGACAAAGCGATGAAT
CCAGCGAAACAACCAGCGACAGGTAAAGTTGTATTGTTACCAACGCATAGAGGAACTGTT
AGTAGCGGTACAGAAGGTTCTGGTCGCACATTAGAAGGAGCTACTGTATCAAGCAAGAGT
GGGAACCAATTGGTTAGAATGTCAGTGCCTAAAGGTAGCGCGCATGAGAAACAGTTACCA
AAAACTGGAACTAATCAAAGCTCAAGCCCAGCAGCGATGTTTGTATTAGTAGCAGGTATA
GGTTTAATCGCGACTGTACGACGTAGAAAAGCTAGTTAA60
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⊟Protein[edit | edit source]
⊟General[edit | edit source]
- locus tag: SA0022 [new locus tag: SA_RS00265 ]
- symbol: SA0022
- description: hypothetical protein
- length: 772
- theoretical pI: 9.82404
- theoretical MW: 83497.9
- GRAVY: -0.448964
⊟Function[edit | edit source]
- TIGRFAM: Biosynthesis of cofactors, prosthetic groups, and carriers Pyridine nucleotides NAD nucleotidase (TIGR01530; EC 3.1.3.5,3.6.1.22; HMM-score: 135.2)Transport and binding proteins Other NAD nucleotidase (TIGR01530; EC 3.1.3.5,3.6.1.22; HMM-score: 135.2)and 5 morethiosulfohydrolase SoxB (TIGR04486; HMM-score: 92.1)Purines, pyrimidines, nucleosides, and nucleotides Other 2',3'-cyclic-nucleotide 2'-phosphodiesterase (TIGR01390; EC 3.1.4.16; HMM-score: 53.1)Cell envelope Other LPXTG cell wall anchor domain (TIGR01167; HMM-score: 25)DNA metabolism DNA replication, recombination, and repair exonuclease SbcCD, D subunit (TIGR00619; HMM-score: 14.3)VPLPA-CTERM protein sorting domain (TIGR03370; HMM-score: 10.6)
- TheSEED :
- 5'-nucleotidase (EC 3.1.3.5)
- Virulence-associated cell-wall-anchored protein SasH (LPXTG motif)
and 2 more - PFAM: no clan defined 5_nucleotid_C; 5'-nucleotidase, C-terminal domain (PF02872; HMM-score: 141.2)and 4 moreCalcineurin (CL0163) Metallophos; Calcineurin-like phosphoesterase (PF00149; HMM-score: 44.1)no clan defined Gram_pos_anchor; LPXTG cell wall anchor motif (PF00746; HMM-score: 14.6)IES5; Ino80 complex subunit 5 (PF17335; HMM-score: 14.6)Cupin (CL0029) AraC_N; AraC-type transcriptional regulator N-terminus (PF06719; HMM-score: 13.3)
⊟Structure, modifications & cofactors[edit | edit source]
- domains:
- modifications:
- cofactors:
- effectors:
⊟Localization[edit | edit source]
- PSORTb: Cellwall
- Cytoplasmic Score: 0
- Cytoplasmic Membrane Score: 0
- Cellwall Score: 10
- Extracellular Score: 0
- Internal Helices: 2
- LocateP: LPxTG Cell-wall anchored
- Prediction by SwissProt Classification: Cell Wall
- Pathway Prediction: Sec-(SPI)
- Intracellular possibility: 0
- Signal peptide possibility: 1
- N-terminally Anchored Score: -2
- Predicted Cleavage Site: found LPxTG motif :LPKTG
- SignalP: Signal peptide SP(Sec/SPI) length 27 aa
- SP(Sec/SPI): 0.994304
- TAT(Tat/SPI): 0.000715
- LIPO(Sec/SPII): 0.002271
- Cleavage Site: CS pos: 27-28. SNA-AE. Pr: 0.9411
- predicted transmembrane helices (TMHMM): 1
⊟Accession numbers[edit | edit source]
⊟Protein sequence[edit | edit source]
- MKALLLKTSVWLVLLFSVMGLWQVSNAAEQYTPIKAHVVTTIDKATTDKQQVTPTKEAAHQFGEEAATNVSASAQGTADEINNKVTSNAFSNKPSTAVSTKVNETHDVDTQQASTQKPTQSATFTLSNAKTASLSPRMFAANVPQTTTHKILHTNDIHGRLAEEKGRVIGMAKLKTIKEQEKPDLMLDAGDAFQGLPLSNQSKGEEMAKAMNAVGYDAMAVGNHEFDFGYDQLKKLEGMLDFPMLSTNVYKDGKRAFKPSTIVTKNGIRYGIIGVTTPETKTKTRPEGIKGVEFRDPLQSVTAEMMRIYKDVDTFVVISHLGIDPSTQETWRGDYLVKQLSQNPQLKKRITVIDGHSHTVLQNGQIYNNDALAQTGTALANIGKVTFNYRNGEVSNIKPSLINVKDVENVTPNKALAEQINQADQTFRAQTAEVIIPNNTIDFKGERDDVRTRETNLGNAIADAMEAYGVKNFSKKTDFAVTNGGGIRASIAKGKVTRYDLISVLPFGNTIAQIDVKGSDVWTAFEHSLGAPTTQKDGKTVLTANGGLLHISDSIRVYYDMNKPSGKRINAIQILNKETGKFENIDLKRVYHVTMNDFTASGGDGYSMFGGPREEGISLDQVLASYLKTANIAKYDTTEPQRMLLGKPAVSEQPAKGQQGSKGSESGKDVQPIGDDKAMNPAKQPATGKVVLLPTHRGTVSSGTEGSGRTLEGATVSSKSGNQLVRMSVPKGSAHEKQLPKTGTNQSSSPAAMFVLVAGIGLIATVRRRKAS
⊟Experimental data[edit | edit source]
⊟Expression & Regulation[edit | edit source]
⊟Operon[edit | edit source]
- MicrobesOnline: no polycistronic organisation predicted
⊟Regulation[edit | edit source]
- regulator:
⊟Transcription pattern[edit | edit source]
- S.aureus Expression Data Browser: data available for NCTC8325
⊟Protein synthesis (provided by Aureolib)[edit | edit source]
- Aureolib: no data available
⊟Protein stability[edit | edit source]
- half-life: no data available
⊟Biological Material[edit | edit source]
⊟Mutants[edit | edit source]
⊟Expression vector[edit | edit source]
⊟lacZ fusion[edit | edit source]
⊟GFP fusion[edit | edit source]
⊟two-hybrid system[edit | edit source]
⊟FLAG-tag construct[edit | edit source]
⊟Antibody[edit | edit source]
⊟Other Information[edit | edit source]
You are kindly invited to share additional interesting facts.
⊟Literature[edit | edit source]
⊟References[edit | edit source]
⊟Relevant publications[edit | edit source]
Vilasack Thammavongsa, Justin W Kern, Dominique M Missiakas, Olaf Schneewind
Staphylococcus aureus synthesizes adenosine to escape host immune responses.
J Exp Med: 2009, 206(11);2417-27
[PubMed:19808256] [WorldCat.org] [DOI] (I p)Vilasack Thammavongsa, Olaf Schneewind, Dominique M Missiakas
Enzymatic properties of Staphylococcus aureus adenosine synthase (AdsA).
BMC Biochem: 2011, 12;56
[PubMed:22035583] [WorldCat.org] [DOI] (I e)Hwan Keun Kim, Vilasack Thammavongsa, Olaf Schneewind, Dominique Missiakas
Recurrent infections and immune evasion strategies of Staphylococcus aureus.
Curr Opin Microbiol: 2012, 15(1);92-9
[PubMed:22088393] [WorldCat.org] [DOI] (I p)