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FunGene: 08-OCT-2024

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus JSNZ
  • locus tag: JSNZ_001982
  • pan locus tag?: SAUPAN005241000
  • symbol: groL
  • pan gene symbol?: groEL
  • synonym:
  • product: chaperonin GroEL

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: JSNZ_001982
  • symbol: groL
  • product: chaperonin GroEL
  • replicon: chromosome
  • strand: -
  • coordinates: 1996527..1998143
  • length: 1617
  • essential: unknown other strains

⊟Accession numbers[edit | edit source]

  • Gene ID:
  • RefSeq:
  • BioCyc:
  • MicrobesOnline:

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    ATGGTTAAACAATTGAAATTCTCTGAAGATGCGCGTCAAGCAATGTTACGTGGTGTTGAC
    CAACTTGCAAATGCAGTTAAAGTAACGATTGGTCCTAAAGGACGTAATGTTGTATTAGAT
    AAAGAGTTTACAGCACCTTTAATTACGAACGATGGTGTAACGATTGCTAAAGAAATCGAA
    TTAGAAGATCCATATGAAAATATGGGGGCTAAACTAGTTCAAGAAGTCGCAAATAAGACA
    AATGAAATTGCTGGTGACGGTACGACAACTGCAACAGTATTAGCTCAAGCAATGATTCAA
    GAAGGCTTGAAAAATGTTACAAGTGGTGCGAACCCAGTTGGTTTACGACAAGGTATCGAC
    AAAGCAGTTAAAGTTGCTGTTGAAGCGTTACATGAAAATTCTCAAAAAGTTGAAAATAAA
    AATGAAATTGCGCAAGTAGGTGCGATTTCAGCAGCAGATGAAGAAATTGGACGTTATATT
    TCTGAAGCTATGGAAAAAGTAGGTAACGATGGTGTCATTACAATTGAAGAATCAAATGGA
    CTAAACACTGAACTAGAAGTGGTTGAAGGTATGCAATTTGATCGTGGTTATCAATCACCG
    TATATGGTTACTGATTCAGATAAAATGGTTGCTGAATTAGAACGCCCATACATTTTAGTA
    ACAGATAAGAAAATCTCGTCTTTCCAAGATATCTTACCTTTATTAGAACAAGTGGTTCAA
    TCTAATCGTCCAATCTTAATTGTAGCTGATGAAGTTGAAGGCGATGCATTAACAAATATC
    GTGCTAAACCGTATGCGTGGCACATTTACAGCTGTTGCAGTAAAGGCGCCTGGTTTCGGT
    GATCGTCGTAAAGCAATGTTAGAAGATTTAGCTATTTTAACTGGTGCGCAAGTGATTACT
    GATGATTTAGGCTTAGATTTAAAAGATGCATCAATTGATATGTTAGGTACTGCAAGTAAA
    GTAGAAGTAACTAAAGATAATACCACTGTTGTTGATGGTGACGGTGACGAAAACAGCATT
    GATGCACGTGTTAGCCAATTGAAATCTCAAATTGAAGAAACTGAATCTGACTTTGATCGT
    GAAAAATTACAAGAGCGCTTAGCTAAATTAGCAGGTGGTGTTGCAGTTATCAAAGTAGGT
    GCAGCAAGTGAAACAGAGCTTAAAGAACGTAAATTACGTATTGAAGATGCATTAAATTCT
    ACACGTGCAGCAGTTGAAGAAGGTATTGTTGCAGGTGGTGGTACTGCATTAGTAAATGTT
    TACCAAAAAGTAAGTGAAATTGAAGCTGAAGGTGACATTGAAACAGGTGTAAACATTGTA
    CTTAAAGCACTAACTGCACCAGTTCGTCAAATTGCTGAAAATGCAGGATTAGAAGGTTCT
    GTTATTGTAGAACGTTTGAAAAACGCAGAGCCGGGTGTTGGTTTTAACGCTGCTACAAAC
    GAGTGGGTTAATATGTTAGAAGCAGGTATCGTTGATCCAACTAAAGTAACACGCTCAGCA
    TTACAACATGCTGCAAGTGTTGCAGCAATGTTCTTAACGACTGAAGCGGTTGTGGCATCA
    ATTCCAGAAAAAAATAATGACCAACCTAACATGGGTGGCATGCCAGGAATGATGTAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1200
    1260
    1320
    1380
    1440
    1500
    1560
    1617

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: JSNZ_001982
  • symbol: GroL
  • description: chaperonin GroEL
  • length: 538
  • theoretical pI: 4.28546
  • theoretical MW: 57571.8
  • GRAVY: -0.1671

⊟Function[edit | edit source]

  • TIGRFAM:
    Genetic information processing Protein fate Protein folding and stabilization chaperonin GroL (TIGR02348; HMM-score: 825.1)
    and 10 more
    Genetic information processing Protein fate Protein folding and stabilization thermosome, various subunits, archaeal (TIGR02339; HMM-score: 100.4)
    T-complex protein 1, delta subunit (TIGR02342; HMM-score: 89.4)
    T-complex protein 1, eta subunit (TIGR02345; HMM-score: 79.3)
    T-complex protein 1, epsilon subunit (TIGR02343; HMM-score: 74.7)
    T-complex protein 1, zeta subunit (TIGR02347; HMM-score: 71.2)
    T-complex protein 1, beta subunit (TIGR02341; HMM-score: 70.3)
    T-complex protein 1, gamma subunit (TIGR02344; HMM-score: 63.9)
    T-complex protein 1, theta subunit (TIGR02346; HMM-score: 59.7)
    T-complex protein 1, alpha subunit (TIGR02340; HMM-score: 50.1)
    L-seryl-tRNA(Sec) kinase (TIGR03575; EC 2.7.1.164; HMM-score: 15)
  • TheSEED: data available for COL, N315, NCTC8325, Newman, USA300_FPR3757
  • PFAM:
    no clan defined Cpn60_TCP1; TCP-1/cpn60 chaperonin family (PF00118; HMM-score: 260.4)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 9.97
    • Cytoplasmic Membrane Score: 0
    • Cellwall Score: 0.01
    • Extracellular Score: 0.02
    • Internal Helices: 0
  • DeepLocPro: Cytoplasmic
    • Cytoplasmic Score: 0.983
    • Cytoplasmic Membrane Score: 0.0017
    • Cell wall & surface Score: 0.0009
    • Extracellular Score: 0.0143
  • LocateP:
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.004121
    • TAT(Tat/SPI): 0.001123
    • LIPO(Sec/SPII): 0.000853
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

  • GI:
  • RefSeq:
  • UniProt:

⊟Protein sequence[edit | edit source]

  • MVKQLKFSEDARQAMLRGVDQLANAVKVTIGPKGRNVVLDKEFTAPLITNDGVTIAKEIELEDPYENMGAKLVQEVANKTNEIAGDGTTTATVLAQAMIQEGLKNVTSGANPVGLRQGIDKAVKVAVEALHENSQKVENKNEIAQVGAISAADEEIGRYISEAMEKVGNDGVITIEESNGLNTELEVVEGMQFDRGYQSPYMVTDSDKMVAELERPYILVTDKKISSFQDILPLLEQVVQSNRPILIVADEVEGDALTNIVLNRMRGTFTAVAVKAPGFGDRRKAMLEDLAILTGAQVITDDLGLDLKDASIDMLGTASKVEVTKDNTTVVDGDGDENSIDARVSQLKSQIEETESDFDREKLQERLAKLAGGVAVIKVGAASETELKERKLRIEDALNSTRAAVEEGIVAGGGTALVNVYQKVSEIEAEGDIETGVNIVLKALTAPVRQIAENAGLEGSVIVERLKNAEPGVGFNAATNEWVNMLEAGIVDPTKVTRSALQHAASVAAMFLTTEAVVASIPEKNNDQPNMGGMPGMM

⊟Experimental data[edit | edit source]

  • experimentally validated: data available for COL, NCTC8325
  • protein localization: data available for COL
  • quantitative data / protein copy number per cell: data available for COL
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulators: CtsR* (repression) regulon, HrcA (repression) regulon
    CtsR*(TF)important in Heat shock response;  regulation predicted or transferred from N315 and NCTC 8325  [2]
    HrcA(TF)important in Heat shock response;  regulation predicted or transferred from N315 and NCTC 8325  [2]

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You can add further information about the gene and protein here. [edit]

⊟Literature[edit | edit source]

⊟References[edit | edit source]

  1. ↑ Blanca Taboada, Karel Estrada, Ricardo Ciria, Enrique Merino
    Operon-mapper: a web server for precise operon identification in bacterial and archaeal genomes.
    Bioinformatics: 2018, 34(23);4118-4120
    [PubMed:29931111] [WorldCat.org] [DOI] (I p)
  2. ↑ 2.0 2.1 Hannes Wolfgramm, Larissa Milena Busch, Jöran Tebben, Henry Mehlan, Lisa Hagenau, Thomas Sura, Tilly Hoffmüller, Elisa Bludau, Manuela Gesell Salazar, Alexander Reder, Stephan Michalik, Leif Steil, Kristin Surmann, Ulrike Mäder, Silva Holtfreter, Uwe Völker
    Integrated genomic and proteomic analysis of the mouse-adapted Staphylococcus aureus strain JSNZ.
    Curr Res Microb Sci: 2025, 9;100489
    [PubMed:41146725] [WorldCat.org] [DOI] (I e)

⊟Relevant publications[edit | edit source]

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