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FunGene: 08-OCT-2024

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus JSNZ
  • locus tag: JSNZ_002052
  • pan locus tag?: SAUPAN005373000
  • symbol: thiE
  • pan gene symbol?: thiE
  • synonym:
  • product: thiamine phosphate synthase

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: JSNZ_002052
  • symbol: thiE
  • product: thiamine phosphate synthase
  • replicon: chromosome
  • strand: -
  • coordinates: 2068540..2069181
  • length: 642
  • essential: unknown other strains

⊟Accession numbers[edit | edit source]

  • Gene ID:
  • RefSeq:
  • BioCyc:
  • MicrobesOnline:

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    ATGTTTAACCAATCGTATCTAAATGTGTATTTTATTTGTGGGACCTCCGATGTTCCGAGT
    CATCGAACTATTCATGAAGTATTAGAAGCAGCATTAAAGGCAGGGATTACACTTTTCCAA
    TTTCGAGAAAAGGGTGAATCAGCTTTAAAAGGAAACGATAAACTTGTATTAGCTAAAGAA
    TTACAGCATCTCTGTCATCAATATGATGTGCCTTTTATCGTTAATGATGATGTATCCTTG
    GCAAAAGAAATTAACGCGGATGGTATACATGTCGGTCAAGATGATGCGAAAGTCAAAGAG
    ATAGCACAATATTTCACTGATAAAATTATTGGACTTAGTATTAGTGATTTAGATGAGTAT
    GCAAAATCTGATTTAACTCATGTTGATTATATAGGTGTTGGACCAATCTATCCTACGCCG
    TCGAAGCATGACGCGCATATACCAGTAGGTCCAGAAATGATTGCAACGTTTAAAGAAATG
    AATCCGCAACTACCGATTGTAGCTATTGGCGGTATCAATACTAATAATGTGGCGCCGATT
    GTAGAAGCAGGTGCCAATGGAATTTCAGTCATTTCAGCCATTTCTAAAAGTGAAAATATT
    GAAAAGACTGTTAATCGATTCAAAGATTTTTTTAATAATTAG
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    642

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: JSNZ_002052
  • symbol: ThiE
  • description: thiamine phosphate synthase
  • length: 213
  • theoretical pI: 5.06131
  • theoretical MW: 23398.4
  • GRAVY: -0.110798

⊟Function[edit | edit source]

  • reaction:
    EC 2.5.1.3?  ExPASy
    Thiamine phosphate synthase 4-amino-2-methyl-5-diphosphomethylpyrimidine + 2-((2R,5Z)-2-carboxy-4-methylthiazol-5(2H)-ylidene)ethyl phosphate = diphosphate + thiamine phosphate + CO2 4-amino-2-methyl-5-diphosphomethylpyrimidine + 2-(2-carboxy-4-methylthiazol-5-yl)ethyl phosphate = diphosphate + thiamine phosphate + CO2 4-amino-2-methyl-5-diphosphomethylpyrimidine + 4-methyl-5-(2-phosphono-oxyethyl)thiazole = diphosphate + thiamine phosphate
  • TIGRFAM:
    Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Thiamine thiamine-phosphate diphosphorylase (TIGR00693; EC 2.5.1.3; HMM-score: 211.9)
    and 6 more
    Metabolism Energy metabolism Pentose phosphate pathway ribulose-phosphate 3-epimerase (TIGR01163; EC 5.1.3.1; HMM-score: 20.4)
    phosphoglycerol geranylgeranyltransferase (TIGR01769; EC 2.5.1.41; HMM-score: 18)
    Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Pyridoxine pyridoxal 5'-phosphate synthase, synthase subunit Pdx1 (TIGR00343; HMM-score: 15.2)
    geranylgeranylglyceryl phosphate synthase family protein (TIGR01768; EC 2.5.1.-; HMM-score: 13.7)
    3-hexulose-6-phosphate synthase (TIGR03128; EC 4.1.2.43; HMM-score: 12.8)
    Genetic information processing Protein synthesis tRNA aminoacylation O-phosphoseryl-tRNA(Sec) selenium transferase (TIGR03531; EC 2.9.1.2; HMM-score: 11)
  • TheSEED: data available for COL, N315, NCTC8325, Newman, USA300_FPR3757
  • PFAM:
    TIM_barrel (CL0036) TMP-TENI; Thiamine monophosphate synthase (PF02581; HMM-score: 203)
    and 7 more
    DUF561; Protein of unknown function (DUF561) (PF04481; HMM-score: 24.3)
    ThiG; Thiazole biosynthesis protein ThiG (PF05690; HMM-score: 18.7)
    PLP_aminotran (CL0061) SepSecS; O-phosphoseryl-tRNA(Sec) selenium transferase, SepSecS (PF05889; HMM-score: 18.2)
    TIM_barrel (CL0036) Ribul_P_3_epim; Ribulose-phosphate 3 epimerase family (PF00834; HMM-score: 13.8)
    no clan defined BATS; Biotin and Thiamin Synthesis associated domain (PF06968; HMM-score: 13.8)
    TIM_barrel (CL0036) QRPTase_C; Quinolinate phosphoribosyl transferase, C-terminal domain (PF01729; HMM-score: 13.2)
    DHO_dh; Dihydroorotate dehydrogenase (PF01180; HMM-score: 13)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 7.5
    • Cytoplasmic Membrane Score: 1.15
    • Cellwall Score: 0.62
    • Extracellular Score: 0.73
    • Internal Helices: 0
  • DeepLocPro: Cytoplasmic
    • Cytoplasmic Score: 0.997
    • Cytoplasmic Membrane Score: 0.0017
    • Cell wall & surface Score: 0.0004
    • Extracellular Score: 0.0009
  • LocateP:
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.005081
    • TAT(Tat/SPI): 0.000633
    • LIPO(Sec/SPII): 0.001509
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

  • GI:
  • RefSeq:
  • UniProt:

⊟Protein sequence[edit | edit source]

  • MFNQSYLNVYFICGTSDVPSHRTIHEVLEAALKAGITLFQFREKGESALKGNDKLVLAKELQHLCHQYDVPFIVNDDVSLAKEINADGIHVGQDDAKVKEIAQYFTDKIIGLSISDLDEYAKSDLTHVDYIGVGPIYPTPSKHDAHIPVGPEMIATFKEMNPQLPIVAIGGINTNNVAPIVEAGANGISVISAISKSENIEKTVNRFKDFFNN

⊟Experimental data[edit | edit source]

  • experimentally validated: data available for COL, NCTC8325
  • protein localization: data available for COL
  • quantitative data / protein copy number per cell:
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • data available for N315

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You can add further information about the gene and protein here. [edit]

⊟Literature[edit | edit source]

⊟References[edit | edit source]

  1. ↑ Blanca Taboada, Karel Estrada, Ricardo Ciria, Enrique Merino
    Operon-mapper: a web server for precise operon identification in bacterial and archaeal genomes.
    Bioinformatics: 2018, 34(23);4118-4120
    [PubMed:29931111] [WorldCat.org] [DOI] (I p)

⊟Relevant publications[edit | edit source]

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