PangenomeCOLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 06-JUL-2013

⊟Summary

• organism: Staphylococcus aureus Newman
• locus tag: NWMN_1995 [new locus tag: NWMN_RS11545 ]
• pan locus tag^?: SAUPAN005373000
• symbol: thiE
• pan gene symbol^?: thiE
• synonym:
• product: thiamine-phosphate pyrophosphorylase

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: NWMN_1995 [new locus tag: NWMN_RS11545 ]
• symbol: thiE
• product: thiamine-phosphate pyrophosphorylase
• replicon: chromosome
• strand: -
• coordinates: 2216965..2217606
• length: 642
• essential: unknown other strains

⊟Accession numbers

• Gene ID: 5332232 NCBI
• RefSeq: YP_001333029 NCBI
• BioCyc:
• MicrobesOnline: 3707588 MicrobesOnline

⊟Phenotype

Share your knowledge and add information here. [edit]

⊟DNA sequence

• 1
  61
  121
  181
  241
  301
  361
  421
  481
  541
  601
  
  ATGTTTAACCAATCGTATCTAAATGTGTATTTTATTTGTGGGACCTCCGATGTTCCGAGT
  CATCGAACTATTCATGAAGTATTAGAAGCAGCATTAAAGGCAGGGATTACACTTTTCCAA
  TTTCGAGAAAAGGGTGAATCAGCTTTAAAAGGAAACGATAAACTTGTATTAGCTAAAGAA
  TTACAGCATCTCTGTCATCAATATGATGTGCCTTTTATCGTTAATGATGATGTATCCTTG
  GCAAAAGAAATTAACGCGGATGGTATACATGTCGGTCAAGATGATGCGAAAGTCAAAGAG
  ATAGCACAATATTTCACTGATAAAATTATTGGACTTAGTATTAGTGATTTAGATGAGTAT
  GCAAAATCTGATTTAACTCATGTTGATTATATAGGTGTTGGACCAATCTATCCTACGCCG
  TCGAAGCATGACGCGCATATACCAGTAGGTCCAGAAATGATTGCAACGTTTAAAGAAATG
  AATCCGCAACTACCGATTGTAGCTATTGGCGGTATCAATACTAATAATGTGGCGCCGATT
  GTAGAAGCAGGTGCCAATGGAATTTCAGTCATTTCAGCCATTTCTAAAAGTGAAAATATT
  GAAAAGACTGTTAATCGATTCAAAGATTTTTTTAATAATTAG
  60
  120
  180
  240
  300
  360
  420
  480
  540
  600
  642
  

⊟Protein

⊟General

• locus tag: NWMN_1995 [new locus tag: NWMN_RS11545 ]
• symbol: ThiE
• description: thiamine-phosphate pyrophosphorylase
• length: 213
• theoretical pI: 5.06131
• theoretical MW: 23398.4
• GRAVY: -0.110798

⊟Function

• reaction:
  EC 2.5.1.3?  ExPASy
  Thiamine phosphate synthase 4-amino-2-methyl-5-diphosphomethylpyrimidine + 2-((2R,5Z)-2-carboxy-4-methylthiazol-5(2H)-ylidene)ethyl phosphate = diphosphate + thiamine phosphate + CO₂ 4-amino-2-methyl-5-diphosphomethylpyrimidine + 2-(2-carboxy-4-methylthiazol-5-yl)ethyl phosphate = diphosphate + thiamine phosphate + CO₂ 4-amino-2-methyl-5-diphosphomethylpyrimidine + 4-methyl-5-(2-phosphono-oxyethyl)thiazole = diphosphate + thiamine phosphate
• TIGRFAM:
  Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Thiamine thiamine-phosphate diphosphorylase (TIGR00693; EC 2.5.1.3; HMM-score: 211.9)
  and 6 more
  Metabolism Energy metabolism Pentose phosphate pathway ribulose-phosphate 3-epimerase (TIGR01163; EC 5.1.3.1; HMM-score: 20.4)
  phosphoglycerol geranylgeranyltransferase (TIGR01769; EC 2.5.1.41; HMM-score: 18)
  Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Pyridoxine pyridoxal 5'-phosphate synthase, synthase subunit Pdx1 (TIGR00343; HMM-score: 15.2)
  geranylgeranylglyceryl phosphate synthase family protein (TIGR01768; EC 2.5.1.-; HMM-score: 13.7)
  3-hexulose-6-phosphate synthase (TIGR03128; EC 4.1.2.43; HMM-score: 12.8)
  Genetic information processing Protein synthesis tRNA aminoacylation O-phosphoseryl-tRNA(Sec) selenium transferase (TIGR03531; EC 2.9.1.2; HMM-score: 11)
• TheSEED: data available for COL, N315, NCTC8325, USA300_FPR3757
• PFAM:
  TIM_barrel (CL0036) TMP-TENI; Thiamine monophosphate synthase (PF02581; HMM-score: 199.1)
  and 8 more
  DUF561; Protein of unknown function (DUF561) (PF04481; HMM-score: 24.4)
  ThiG; Thiazole biosynthesis protein ThiG (PF05690; HMM-score: 19.3)
  PLP_aminotran (CL0061) SepSecS; O-phosphoseryl-tRNA(Sec) selenium transferase, SepSecS (PF05889; HMM-score: 18.4)
  TIM_barrel (CL0036) NanE; Putative N-acetylmannosamine-6-phosphate epimerase (PF04131; HMM-score: 15.9)
  Ribul_P_3_epim; Ribulose-phosphate 3 epimerase family (PF00834; HMM-score: 13.8)
  DHO_dh; Dihydroorotate dehydrogenase (PF01180; HMM-score: 13.6)
  QRPTase_C; Quinolinate phosphoribosyl transferase, C-terminal domain (PF01729; HMM-score: 12.9)
  Peptidase_CA (CL0125) Peptidase_C78; Peptidase family C78 (PF07910; HMM-score: 12.7)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors: Mg²⁺
• effectors:

⊟Localization

• PSORTb: Cytoplasmic
  • Cytoplasmic Score: 7.5
  • Cytoplasmic Membrane Score: 1.15
  • Cellwall Score: 0.62
  • Extracellular Score: 0.73
  • Internal Helices: 0
• LocateP: Intracellular
  • Prediction by SwissProt Classification: Cytoplasmic
  • Pathway Prediction: No pathway
  • Intracellular possibility: 1
  • Signal peptide possibility: -1
  • N-terminally Anchored Score: 1
  • Predicted Cleavage Site: No CleavageSite
• SignalP: no predicted signal peptide
  • SP(Sec/SPI): 0.005081
  • TAT(Tat/SPI): 0.000633
  • LIPO(Sec/SPII): 0.001509
• predicted transmembrane helices (TMHMM): 0

⊟Accession numbers

• GI: 151222207 NCBI
• RefSeq: YP_001333029 NCBI
• UniProt: A6QIT5 UniProt

⊟Protein sequence

• MFNQSYLNVYFICGTSDVPSHRTIHEVLEAALKAGITLFQFREKGESALKGNDKLVLAKELQHLCHQYDVPFIVNDDVSLAKEINADGIHVGQDDAKVKEIAQYFTDKIIGLSISDLDEYAKSDLTHVDYIGVGPIYPTPSKHDAHIPVGPEMIATFKEMNPQLPIVAIGGINTNNVAPIVEAGANGISVISAISKSENIEKTVNRFKDFFNN

⊟Experimental data

• experimentally validated: data available for COL, NCTC8325
• protein localization: data available for COL
• quantitative data / protein copy number per cell:
• interaction partners:

⊟Expression & Regulation

⊟Operon

• MicrobesOnline: oxaA < thiE < thiM < thiD < NWMN_1998

⊟Regulation

• regulator: THI-box (transcription termination) regulon

  THI-box

  (RNA)

  important in Thiamine biosynthesis; transcription unit transferred from N315 data RegPrecise

⊟Transcription pattern

• S.aureus Expression Data Browser: data available for NCTC8325

⊟Protein synthesis (provided by Aureolib)

• Aureolib: no data available

⊟Protein stability

• half-life: no data available

⊟Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊟Other Information

You are kindly invited to share additional interesting facts.

⊟Literature

⊟References

⊟Relevant publications