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NCBI: 06-JUL-2013

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus Newman
  • locus tag: NWMN_1996 [new locus tag: NWMN_RS11550 ]
  • pan locus tag?: SAUPAN005374000
  • symbol: thiM
  • pan gene symbol?: thiM
  • synonym:
  • product: hydroxyethylthiazole kinase

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: NWMN_1996 [new locus tag: NWMN_RS11550 ]
  • symbol: thiM
  • product: hydroxyethylthiazole kinase
  • replicon: chromosome
  • strand: -
  • coordinates: 2217608..2218399
  • length: 792
  • essential: unknown other strains

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    ATGAATTATCTAAATAAAATACGTATTGAAAACCCATTAACAATTTGTTATACAAACGAT
    GTAGTTAAAAATTTTACAGCGAATGGTTTATTAAGTATTGGTGCTAGCCCTGCAATGAGT
    GAAGCTCCCGAAGAAGCTGAAGAATTTTACAAAGTTGCACAAGCGCTATTAATCAATATC
    GGTACTTTAACAGCAGAAAATGAACAAGATATTATTGCGATTGCTCAAACGGCAAATGAG
    GCAGGCTTACCTATTGTATTTGACCCTGTAGCTGTTGGTGCTTCTACATATCGAAAGCAA
    TTTTGTAAATTATTATTGAAATCAGCGAAAGTATCAGTAATTAAAGGCAATGCATCTGAA
    ATATTAGCGTTGATTGATGATACAGCAACTATGAAAGGTACAGATAGTGATGCTAATCTT
    GATGCGGTTGCAATAGCGAAAAAGGCTTACGCAACATATAAAACTGCAATAGTAATCACA
    GGTAAAGAGGACGTTATTGTTCAAGATAATAAAGCCTTCGTATTAGCTAATGGATCTCCA
    TTATTAGCACGAGTAACTGGAGCTGGTTGTTTATTAGGAGGCGTTATTGCTGGATTTTTA
    TTTAGAGAAACAGAACCAGACATAGAAGCGTTAATTGAAGCGGTAAGCGTATTTAATATT
    GCTGCTGAGGTAGCTGCTGAAAATGAAAATTGTGGTGGTCCTGGTACGTTTTCACCATTG
    TTGCTTGATACGTTATATCATTTAAATGAAACAACCTATCAACAACGGATTCGTATTCAA
    GAGGTGGAATAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    792

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: NWMN_1996 [new locus tag: NWMN_RS11550 ]
  • symbol: ThiM
  • description: hydroxyethylthiazole kinase
  • length: 263
  • theoretical pI: 4.28293
  • theoretical MW: 28089.9
  • GRAVY: 0.176046

⊟Function[edit | edit source]

  • reaction:
    EC 2.7.1.50?  ExPASy
    Hydroxyethylthiazole kinase ATP + 4-methyl-5-(2-hydroxyethyl)thiazole = ADP + 4-methyl-5-(2-phosphonooxyethyl)thiazole
  • TIGRFAM:
    Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Thiamine hydroxyethylthiazole kinase (TIGR00694; EC 2.7.1.50; HMM-score: 256.6)
    and 3 more
    Unknown function General YjeF family C-terminal domain (TIGR00196; HMM-score: 38.5)
    methyltransferase, MtaA/CmuA family (TIGR01463; EC 2.1.1.-; HMM-score: 13.6)
    Metabolism Transport and binding proteins Carbohydrates, organic alcohols, and acids oligosaccharide:H+ symporter (TIGR00882; HMM-score: 12.8)
  • TheSEED: data available for COL, N315, NCTC8325, USA300_FPR3757
  • PFAM:
    Ribokinase (CL0118) HK; Hydroxyethylthiazole kinase family (PF02110; HMM-score: 256.6)
    and 4 more
    Carb_kinase; Carbohydrate kinase (PF01256; HMM-score: 37.3)
    Phos_pyr_kin; Phosphomethylpyrimidine kinase (PF08543; HMM-score: 31.6)
    no clan defined HasA; Heme-binding protein A (HasA) (PF06438; HMM-score: 12.6)
    MFS (CL0015) LacY_symp; LacY proton/sugar symporter (PF01306; HMM-score: 12.5)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors: Mg2+
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: unknown (no significant prediction)
    • Cytoplasmic Score: 2.5
    • Cytoplasmic Membrane Score: 2.5
    • Cellwall Score: 2.5
    • Extracellular Score: 2.5
    • Internal Helix: 1
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular possibility: 1
    • Signal peptide possibility: -1
    • N-terminally Anchored Score: -2
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.055371
    • TAT(Tat/SPI): 0.015875
    • LIPO(Sec/SPII): 0.001796
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MNYLNKIRIENPLTICYTNDVVKNFTANGLLSIGASPAMSEAPEEAEEFYKVAQALLINIGTLTAENEQDIIAIAQTANEAGLPIVFDPVAVGASTYRKQFCKLLLKSAKVSVIKGNASEILALIDDTATMKGTDSDANLDAVAIAKKAYATYKTAIVITGKEDVIVQDNKAFVLANGSPLLARVTGAGCLLGGVIAGFLFRETEPDIEALIEAVSVFNIAAEVAAENENCGGPGTFSPLLLDTLYHLNETTYQQRIRIQEVE

⊟Experimental data[edit | edit source]

  • experimentally validated: data available for COL, NCTC8325
  • protein localization: data available for COL
  • quantitative data / protein copy number per cell:
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator: THI-box (transcription termination) regulon
    THI-box(RNA)important in Thiamine biosynthesis; transcription unit transferred from N315 data RegPrecise 

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

⊟Relevant publications[edit | edit source]

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