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FunGene: 08-OCT-2024

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus JSNZ
  • locus tag: JSNZ_002218
  • pan locus tag?: SAUPAN005706000
  • symbol: JSNZ_002218
  • pan gene symbol?: topB
  • synonym:
  • product: DNA topoisomerase III

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: JSNZ_002218
  • symbol: JSNZ_002218
  • product: DNA topoisomerase III
  • replicon: chromosome
  • strand: -
  • coordinates: 2218445..2220580
  • length: 2136
  • essential: unknown other strains

⊟Accession numbers[edit | edit source]

  • Gene ID:
  • RefSeq:
  • BioCyc:
  • MicrobesOnline:

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    ATGAAATCTTTAATATTAGCTGAAAAACCATCAGTCGCAAGAGATATTGCTGATGCTTTA
    CAAATAAATCAGAAGCGTAATGGTTACTTTGAAAATAACCAATATATTGTCACGTGGGCG
    TTAGGTCATCTAGTGACAAATGCGACACCTGAACAATACGATAAAAATTTAAAGGAATGG
    CGATTAGAAGACCTTCCAATTATACCTAAATATATGAAAACTGTTGTTATTGGTAAAACA
    AGCAAACAATTTAAAACAGTAAAAGCGTTAATTTTAGATAATAAAGTGAAAGATATTATT
    ATTGCAACAGATGCTGGACGAGAAGGTGAACTAGTTGCAAGATTGATTTTGGATAAAGTT
    GGTAACAAAAAGCCAATCCGTCGATTATGGATTAGCTCAGTTACTAAAAAAGCTATTCAA
    CAAGGTTTTAAAAATTTAAAAGACGGTCGTCAATATAACGATTTGTATTATGCAGCGTTA
    GCGAGAAGTGAGGCAGATTGGATTGTTGGGATTAATGCAACGCGTGCACTAACAACAAAG
    TATGATGCACAGCTATCCCTGGGACGTGTTCAGACACCAACGATTCAATTAGTAAATACA
    CGACAACAAGAGATTAATCAGTTCAAACCACAACAATACTTTACATTATCATTAACGGTA
    AAAGGGTTTGATTTTCAGCTAGAATCAAATCAGCGATATACCAATAAAGAAACTTTAGAA
    CAGATGGTTAATAATTTGAAAAATGTCGATGGTAAGATTAAATCTGTTGCTACTAAACAT
    AAGAAGTCGTATCCGCAATCACTGTACAATTTAACAGATTTACAACAAGATATGTATAGA
    CGTTATAAAATTGGACCTAAAGAAACATTGAATACACTTCAAAGCTTATATGAGAGACAT
    AAAGTCGTAACCTATCCAAGAACAGATTCAAACTATTTAACAACTGATATGGTAGATACT
    ATGAAAGAACGTATTCAGGCGACGATGGCAACAACATATAAAGACCAAGCACGCCCATTA
    ATGTCTAAAACATTTTCATCAAAAATGTCGATATTTAATAATCAAAAAGTATCTGATCAC
    CATGCAATTATTCCTACAGAAGTGAGACCTGTCATGTCAGACTTAAGTAATAGAGAATTA
    AAGTTATACGATATGATTGTCGAGCGTTTTTTAGAAGCTTTAATGCCTCCGCACGAGTAT
    GACGCGATAACTGTAACTTTAGAGGTTGCAGGGCACACATTTGTTTTGAAAGAGAATGTA
    ACAACTGTTTTAGGTTTTAAATCTATTAGACAAGGTGAATCTATTACAGAGATGCAACAG
    CCTTTTTCAGAAGGCGATGAAGTGAAGATTTCAAAAACAAATATTAGAGAACATGAAACA
    ACACCTCCAGAATATTTTAATGAAGGTTCGTTATTAAAAGCGATGGAGAACCCTCAGAAC
    TTTATTCAATTGAAGGATAAAAAATATGCGCAAACTTTAAAACAAACAGGTGGTATCGGC
    ACAGTTGCAACAAGGGCCGACATTATCGATAAATTATTTAATATGAATGCCATTGAATCA
    AGAGACGGTAAAATTAAAGTAACGTCAAAAGGTAAACAAATATTAGAATTAGCACCAGAA
    GAATTAACGTCGCCACTTTTAACTGCACAATGGGAAGAAAAATTACTTTTAATTGAACGT
    GGTAAATATCAGGCGAAAACATTCATTAATGAAATGAAAGATTTTACGAAAGATGTTGTA
    AATGGGATTAAAAATAGTGATCGTAAATATAAACACGATAATTTAACAACCACAGAATGC
    CCAACGTGTGGTAAATTCATGATTAAAGTTAAAACTAAAAATGGTCAGATGCTTGTGTGC
    CAAGATCCATCTTGTAAGACGAAAAAGAATGTACAGCGCAAAACAAATGCAAGATGTCCA
    AACTGTAAAAAGAAATTAACGTTGTTTGGTAAAGGGAAAGAAGCGGTATATCGTTGTGTT
    TGTGGACATTCTGAAACGCAAGCACATATGGATCAGCGTATGAAGTCTAAATCCTCTGGT
    AAAGTATCTCGTAAAGAAATGAAAAAGTATATGAATAAAAATGAAGGTTTAGACAATAAT
    CCGTTTAAAGATGCATTAAAGAACTTGAATTTATAG
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1200
    1260
    1320
    1380
    1440
    1500
    1560
    1620
    1680
    1740
    1800
    1860
    1920
    1980
    2040
    2100
    2136

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: JSNZ_002218
  • symbol: JSNZ_002218
  • description: DNA topoisomerase III
  • length: 711
  • theoretical pI: 10.0831
  • theoretical MW: 81523.4
  • GRAVY: -0.657806

⊟Function[edit | edit source]

  • reaction:
    EC 5.6.2.1?  ExPASy
    non-specified enzyme name
  • TIGRFAM:
    Genetic information processing DNA metabolism DNA replication, recombination, and repair DNA topoisomerase III (TIGR01056; EC 5.99.1.2; HMM-score: 674.4)
    and 4 more
    Genetic information processing DNA metabolism DNA replication, recombination, and repair DNA topoisomerase I (TIGR01057; EC 5.99.1.2; HMM-score: 262.4)
    Genetic information processing DNA metabolism DNA replication, recombination, and repair DNA topoisomerase I (TIGR01051; EC 5.99.1.2; HMM-score: 206.7)
    conserved hypothetical protein (TIGR02443; HMM-score: 14.8)
    Genetic information processing Protein fate Protein modification and repair hydrogenase nickel insertion protein HypA (TIGR00100; HMM-score: 7.2)
  • TheSEED: data available for COL, N315, NCTC8325, Newman, USA300_FPR3757
  • PFAM:
    no clan defined Topoisom_bac; DNA topoisomerase (PF01131; HMM-score: 364.5)
    and 11 more
    Toprim-like (CL0413) Toprim; Toprim domain (PF01751; HMM-score: 52.4)
    Zn_Beta_Ribbon (CL0167) Zn_ribbon_Top1; Topoisomerase DNA binding C4 zinc finger (PF01396; HMM-score: 18.3)
    Zn_ribbon_3; zinc-ribbon domain (PF13248; HMM-score: 13.6)
    Zn_ribbon_ISL3; zinc-finger of transposase IS204/IS1001/IS1096/IS1165 (PF14690; HMM-score: 13.1)
    Toprim-like (CL0413) Toprim_4; Toprim domain (PF13662; HMM-score: 13)
    Zn_Beta_Ribbon (CL0167) DUF7575; Domain of unknown function (DUF7575) (PF24460; HMM-score: 12.1)
    4H_Cytokine (CL0053) Leptin; Leptin (PF02024; HMM-score: 11.1)
    Zn_Beta_Ribbon (CL0167) Zn_ribbon_6; Double zinc ribbon (PF07191; HMM-score: 10.2)
    HVO_2753_ZBP; Small zinc finger protein HVO_2753-like, Zn-binding pocket (PF07754; HMM-score: 10.1)
    Cas12f1-like_TNB; Cas12f1-like, TNB domain (PF07282; HMM-score: 8.6)
    Zn_ribbon_TFIIB; Transcription factor zinc-finger (PF13453; HMM-score: 8)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 9.97
    • Cytoplasmic Membrane Score: 0
    • Cellwall Score: 0.01
    • Extracellular Score: 0.02
    • Internal Helices: 0
  • DeepLocPro: Cytoplasmic
    • Cytoplasmic Score: 0.852
    • Cytoplasmic Membrane Score: 0.0267
    • Cell wall & surface Score: 0.0436
    • Extracellular Score: 0.0777
  • LocateP:
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.010101
    • TAT(Tat/SPI): 0.000561
    • LIPO(Sec/SPII): 0.001039
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

  • GI:
  • RefSeq:
  • UniProt:

⊟Protein sequence[edit | edit source]

  • MKSLILAEKPSVARDIADALQINQKRNGYFENNQYIVTWALGHLVTNATPEQYDKNLKEWRLEDLPIIPKYMKTVVIGKTSKQFKTVKALILDNKVKDIIIATDAGREGELVARLILDKVGNKKPIRRLWISSVTKKAIQQGFKNLKDGRQYNDLYYAALARSEADWIVGINATRALTTKYDAQLSLGRVQTPTIQLVNTRQQEINQFKPQQYFTLSLTVKGFDFQLESNQRYTNKETLEQMVNNLKNVDGKIKSVATKHKKSYPQSLYNLTDLQQDMYRRYKIGPKETLNTLQSLYERHKVVTYPRTDSNYLTTDMVDTMKERIQATMATTYKDQARPLMSKTFSSKMSIFNNQKVSDHHAIIPTEVRPVMSDLSNRELKLYDMIVERFLEALMPPHEYDAITVTLEVAGHTFVLKENVTTVLGFKSIRQGESITEMQQPFSEGDEVKISKTNIREHETTPPEYFNEGSLLKAMENPQNFIQLKDKKYAQTLKQTGGIGTVATRADIIDKLFNMNAIESRDGKIKVTSKGKQILELAPEELTSPLLTAQWEEKLLLIERGKYQAKTFINEMKDFTKDVVNGIKNSDRKYKHDNLTTTECPTCGKFMIKVKTKNGQMLVCQDPSCKTKKNVQRKTNARCPNCKKKLTLFGKGKEAVYRCVCGHSETQAHMDQRMKSKSSGKVSRKEMKKYMNKNEGLDNNPFKDALKNLNL

⊟Experimental data[edit | edit source]

  • experimentally validated: data available for COL, NCTC8325
  • protein localization: data available for COL
  • quantitative data / protein copy number per cell: data available for COL
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator:

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You can add further information about the gene and protein here. [edit]

⊟Literature[edit | edit source]

⊟References[edit | edit source]

  1. ↑ Blanca Taboada, Karel Estrada, Ricardo Ciria, Enrique Merino
    Operon-mapper: a web server for precise operon identification in bacterial and archaeal genomes.
    Bioinformatics: 2018, 34(23);4118-4120
    [PubMed:29931111] [WorldCat.org] [DOI] (I p)

⊟Relevant publications[edit | edit source]

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