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NCBI: 03-AUG-2016

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus NCTC8325
  • locus tag: SAOUHSC_02517
  • pan locus tag?: SAUPAN005706000
  • symbol: SAOUHSC_02517
  • pan gene symbol?: topB
  • synonym:
  • product: DNA topoisomerase III

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SAOUHSC_02517
  • symbol: SAOUHSC_02517
  • product: DNA topoisomerase III
  • replicon: chromosome
  • strand: -
  • coordinates: 2320524..2322659
  • length: 2136
  • essential: no DEG other strains

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    ATGAAATCTTTAATATTAGCTGAAAAACCATCAGTCGCAAGAGATATTGCTGATGCTTTA
    CAAATAAATCAGAAGCGTAATGGTTACTTTGAAAATAACCAATATATTGTCACGTGGGCG
    TTAGGTCATCTAGTGACAAATGCGACACCTGAACAATACGATAAAAATTTAAAGGAATGG
    CGATTAGAAGACCTTCCAATTATACCTAAATATATGAAAACTGTTGTTATTGGTAAAACA
    AGCAAACAATTTAAAACAGTAAAAGCGTTAATTTTAGATAATAAAGTGAAAGATATTATT
    ATTGCAACAGATGCTGGACGAGAAGGTGAACTAGTTGCAAGATTGATTTTGGATAAAGTT
    GGTAACAAAAAGCCAATCCGTCGATTATGGATTAGCTCAGTTACTAAAAAAGCTATTCAA
    CAAGGTTTTAAAAATTTAAAAGACGGTCGTCAATATAACGATTTGTATTATGCAGCGTTA
    GCGAGAAGTGAGGCAGATTGGATTGTTGGGATTAATGCAACGCGTGCACTAACAACAAAG
    TATGATGCACAGCTATCCCTGGGACGTGTTCAGACACCAACGATTCAATTAGTAAATACA
    CGACAACAAGAGATTAATCAGTTCAAACCACAACAATACTTTACATTATCATTAACGGTA
    AAAGGGTTTGATTTTCAGCTAGAATCAAATCAGCGATATACCAATAAAGAAACTTTAGAA
    CAGATGGTTAATAATTTGAAAAATGTCGATGGTAAGATTAAATCTGTTGCTACTAAACAT
    AAGAAGTCGTATCCGCAATCACTGTACAATTTAACAGATTTACAACAAGATATGTATAGA
    CGTTATAAAATTGGACCTAAAGAAACATTGAATACACTTCAAAGCTTATATGAGAGACAT
    AAAGTCGTAACCTATCCAAGAACAGATTCAAACTATTTAACAACTGATATGGTAGATACT
    ATGAAAGAACGTATTCAGGTGACGATGGCAACAACATATAAAGACCAAGCACGCCCATTA
    ATGTCTAAAACATTTTCATCAAAAATGTCGATATTTAATAATCAAAAAGTATCTGATCAC
    CATGCAATTATTCCTACAGAAGTGAGACCTGTCATGTCAGACTTAAGTAATAGAGAATTA
    AAGTTATACGATATGATTGTCGAGCGTTTTTTAGAAGCTTTAATGCCTCCGCACGAGTAT
    GACGCGATAACTGTAACTTTAGAGGTTGCAGGGCACACATTTGTTTTGAAAGAGAATGTA
    ACAACTGTTTTAGGTTTTAAATCTATTAGACAAGGTGAATCTATTACAGAGATGCAACAG
    CCTTTTTCAGAAGGCGATGAAGTGAAGATTTCAAAAACAAATATTAGAGAACATGAAACA
    ACACCTCCAGAATATTTTAATGAAGGTTCGTTATTAAAAGCGATGGAGAACCCTCAGAAC
    TTTATTCAATTGAAGGATAAAAAATATGCGCAAACTTTAAAACAAACAGGTGGTATCGGC
    ACAGTTGCAACAAGGGCCGACATTATCGATAAATTATTTAATATGAATGCCATTGAATCA
    AGAGACGGTAAAATTAAAGTAACGTCAAAAGGTAAACAAATATTAGAATTAGCACCAGAA
    GAATTAACGTCGCCACTTTTAACTGCACAATGGGAAGAAAAATTACTTTTAATTGAACGT
    GGTAAATATCAGGCGAAAACATTTATTAATGAAATGAAAGATTTTACGAAAGATGTTGTA
    AATGGGATTAAAAATAGTGATCGTAAATATAAACACGATAATTTAACAACCACAGAATGC
    CCAACGTGTGGTAAATTCATGATTAAAGTTAAAACTAAAAATGGTCAGATGCTTGTGTGC
    CAAGATCCATCTTGTAAGACGAAAAAGAATGTACAGCGCAAAACAAATGCAAGATGTCCA
    AACTGTAAAAAGAAATTAACGTTGTTTGGTAAAGGGAAAGAAGCGGTATATCGTTGTGTT
    TGTGGACATTCTGAAACGCAAGCACATATGGATCAGCGTATGAAGTCTAAATCCTCTGGT
    AAAGTATCTCGTAAAGAAATGAAAAAGTATATGAATAAAAATGAAGGTTTAGACAATAAT
    CCGTTTAAAGATGCATTAAAGAACTTGAATTTATAG
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1200
    1260
    1320
    1380
    1440
    1500
    1560
    1620
    1680
    1740
    1800
    1860
    1920
    1980
    2040
    2100
    2136

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SAOUHSC_02517
  • symbol: SAOUHSC_02517
  • description: DNA topoisomerase III
  • length: 711
  • theoretical pI: 10.0831
  • theoretical MW: 81551.5
  • GRAVY: -0.65443

⊟Function[edit | edit source]

  • reaction:
    EC 5.99.1.2?  ExPASy
    DNA topoisomerase ATP-independent breakage of single-stranded DNA, followed by passage and rejoining
  • TIGRFAM:
    Genetic information processing DNA metabolism DNA replication, recombination, and repair DNA topoisomerase III (TIGR01056; EC 5.99.1.2; HMM-score: 673)
    and 4 more
    Genetic information processing DNA metabolism DNA replication, recombination, and repair DNA topoisomerase I (TIGR01057; EC 5.99.1.2; HMM-score: 262.4)
    Genetic information processing DNA metabolism DNA replication, recombination, and repair DNA topoisomerase I (TIGR01051; EC 5.99.1.2; HMM-score: 205.8)
    conserved hypothetical protein (TIGR02443; HMM-score: 14.8)
    Genetic information processing Protein fate Protein modification and repair hydrogenase nickel insertion protein HypA (TIGR00100; HMM-score: 7.2)
  • TheSEED  :
    • DNA topoisomerase III (EC 5.99.1.2)
    DNA Metabolism DNA uptake, competence DNA processing cluster  DNA topoisomerase III (EC 5.99.1.2)
  • PFAM:
    no clan defined Topoisom_bac; DNA topoisomerase (PF01131; HMM-score: 370.5)
    and 12 more
    Toprim-like (CL0413) Toprim; Toprim domain (PF01751; HMM-score: 57.3)
    Zn_Beta_Ribbon (CL0167) zf-C4_Topoisom; Topoisomerase DNA binding C4 zinc finger (PF01396; HMM-score: 18.2)
    DUF2387; Probable metal-binding protein (DUF2387) (PF09526; HMM-score: 14.2)
    zf-ribbon_3; zinc-ribbon domain (PF13248; HMM-score: 14)
    Toprim-like (CL0413) Toprim_4; Toprim domain (PF13662; HMM-score: 13)
    no clan defined PXA; PXA domain (PF02194; HMM-score: 12.5)
    4H_Cytokine (CL0053) Leptin; Leptin (PF02024; HMM-score: 11.7)
    no clan defined DUF5416; Family of unknown function (DUF5416) (PF17437; HMM-score: 11.6)
    DNA_ligase_ZBD; NAD-dependent DNA ligase C4 zinc finger domain (PF03119; HMM-score: 10.1)
    HypA; Hydrogenase/urease nickel incorporation, metallochaperone, hypA (PF01155; HMM-score: 9.8)
    Zn_Beta_Ribbon (CL0167) zinc-ribbons_6; zinc-ribbons (PF07191; HMM-score: 9.5)
    zf-TFIIB; Transcription factor zinc-finger (PF13453; HMM-score: 7.1)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors: Mg2+
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 9.97
    • Cytoplasmic Membrane Score: 0
    • Cellwall Score: 0.01
    • Extracellular Score: 0.02
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular possibility: 1
    • Signal peptide possibility: -1
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.010101
    • TAT(Tat/SPI): 0.000561
    • LIPO(Sec/SPII): 0.001039
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MKSLILAEKPSVARDIADALQINQKRNGYFENNQYIVTWALGHLVTNATPEQYDKNLKEWRLEDLPIIPKYMKTVVIGKTSKQFKTVKALILDNKVKDIIIATDAGREGELVARLILDKVGNKKPIRRLWISSVTKKAIQQGFKNLKDGRQYNDLYYAALARSEADWIVGINATRALTTKYDAQLSLGRVQTPTIQLVNTRQQEINQFKPQQYFTLSLTVKGFDFQLESNQRYTNKETLEQMVNNLKNVDGKIKSVATKHKKSYPQSLYNLTDLQQDMYRRYKIGPKETLNTLQSLYERHKVVTYPRTDSNYLTTDMVDTMKERIQVTMATTYKDQARPLMSKTFSSKMSIFNNQKVSDHHAIIPTEVRPVMSDLSNRELKLYDMIVERFLEALMPPHEYDAITVTLEVAGHTFVLKENVTTVLGFKSIRQGESITEMQQPFSEGDEVKISKTNIREHETTPPEYFNEGSLLKAMENPQNFIQLKDKKYAQTLKQTGGIGTVATRADIIDKLFNMNAIESRDGKIKVTSKGKQILELAPEELTSPLLTAQWEEKLLLIERGKYQAKTFINEMKDFTKDVVNGIKNSDRKYKHDNLTTTECPTCGKFMIKVKTKNGQMLVCQDPSCKTKKNVQRKTNARCPNCKKKLTLFGKGKEAVYRCVCGHSETQAHMDQRMKSKSSGKVSRKEMKKYMNKNEGLDNNPFKDALKNLNL

⊟Experimental data[edit | edit source]

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator:

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

  1. ↑ Maren Depke, Stephan Michalik, Alexander Rabe, Kristin Surmann, Lars Brinkmann, Nico Jehmlich, Jörg Bernhardt, Michael Hecker, Bernd Wollscheid, Zhi Sun, Robert L Moritz, Uwe Völker, Frank Schmidt
    A peptide resource for the analysis of Staphylococcus aureus in host-pathogen interaction studies.
    Proteomics: 2015, 15(21);3648-61
    [PubMed:26224020] [WorldCat.org] [DOI] (I p)
  2. ↑ Stephan Michalik, Maren Depke, Annette Murr, Manuela Gesell Salazar, Ulrike Kusebauch, Zhi Sun, Tanja C Meyer, Kristin Surmann, Henrike Pförtner, Petra Hildebrandt, Stefan Weiss, Laura Marcela Palma Medina, Melanie Gutjahr, Elke Hammer, Dörte Becher, Thomas Pribyl, Sven Hammerschmidt, Eric W Deutsch, Samuel L Bader, Michael Hecker, Robert L Moritz, Ulrike Mäder, Uwe Völker, Frank Schmidt
    A global Staphylococcus aureus proteome resource applied to the in vivo characterization of host-pathogen interactions.
    Sci Rep: 2017, 7(1);9718
    [PubMed:28887440] [WorldCat.org] [DOI] (I e)
  3. ↑ 3.00 3.01 3.02 3.03 3.04 3.05 3.06 3.07 3.08 3.09 3.10 3.11 3.12 3.13 3.14 3.15 3.16 3.17 3.18 3.19 3.20 3.21 3.22 3.23 3.24 3.25 3.26 3.27 3.28 3.29 Artem Cherkasov, Michael Hsing, Roya Zoraghi, Leonard J Foster, Raymond H See, Nikolay Stoynov, Jihong Jiang, Sukhbir Kaur, Tian Lian, Linda Jackson, Huansheng Gong, Rick Swayze, Emily Amandoron, Farhad Hormozdiari, Phuong Dao, Cenk Sahinalp, Osvaldo Santos-Filho, Peter Axerio-Cilies, Kendall Byler, William R McMaster, Robert C Brunham, B Brett Finlay, Neil E Reiner
    Mapping the protein interaction network in methicillin-resistant Staphylococcus aureus.
    J Proteome Res: 2011, 10(3);1139-50
    [PubMed:21166474] [WorldCat.org] [DOI] (I p)
  4. ↑ 4.0 4.1 Ulrike Mäder, Pierre Nicolas, Maren Depke, Jan Pané-Farré, Michel Debarbouille, Magdalena M van der Kooi-Pol, Cyprien Guérin, Sandra Dérozier, Aurelia Hiron, Hanne Jarmer, Aurélie Leduc, Stephan Michalik, Ewoud Reilman, Marc Schaffer, Frank Schmidt, Philippe Bessières, Philippe Noirot, Michael Hecker, Tarek Msadek, Uwe Völker, Jan Maarten van Dijl
    Staphylococcus aureus Transcriptome Architecture: From Laboratory to Infection-Mimicking Conditions.
    PLoS Genet: 2016, 12(4);e1005962
    [PubMed:27035918] [WorldCat.org] [DOI] (I e)

⊟Relevant publications[edit | edit source]

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