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FunGene: 08-OCT-2024

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus JSNZ
  • locus tag: JSNZ_002256
  • pan locus tag?: SAUPAN005755000
  • symbol: ureC
  • pan gene symbol?: ureC
  • synonym:
  • product: urease subunit alpha

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: JSNZ_002256
  • symbol: ureC
  • product: urease subunit alpha
  • replicon: chromosome
  • strand: +
  • coordinates: 2252704..2254419
  • length: 1716
  • essential: unknown other strains

⊟Accession numbers[edit | edit source]

  • Gene ID:
  • RefSeq:
  • BioCyc:
  • MicrobesOnline:

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    ATGAGCTTTAAAATGACGCAAAATCAATATACGAGCTTATACGGTCCAACTGTTGGAGAT
    TCTATTCGTTTAGGTGATACGAATCTATTTGCTCAAATAGAAAAAGACTATGCGGTTTAT
    GGTGAAGAAGCTACTTTTGGTGGTGGTAAATCTATTAGAGACGGTATGGCGCAAAATCCT
    CGTGTAACACGTGATGACGTGAACGTTGCAGACCTTGTCATTTCTAATGCCGTTATTATC
    GATTACGATAAAGTGGTTAAAGCTGATATAGGCATTAAAAATGGTTATATTTTCGCCATA
    GGTAATGCCGGCAACCCAGATATAATGGATAATGTCGACATTATTATAGGTTCAACAACA
    GATATCATTGCCGCTGAAGGTAAAATCGTCACTGCTGGTGGTATTGATACTCATGTTCAT
    TTTATTAATCCTGAACAAGCAGAGGTCGCATTAGAAAGTGGTATTACGACTCATATTGGT
    GGTGGTACTGGTGCTTCAGAAGGTTCTAAAGCAACAACTGTAACTCCAGGTCCATGGCAT
    ATTCATAGAATGTTAGAAGCTGCCGAAGGTTTACCGATTAATGTCGGTTTTACAGGTAAA
    GGACAAGCAACAAATCCAACTGCACTCATTGAACAAATCAATGCCGGAGCAATTGGATTA
    AAAGTACATGAAGACTGGGGTGCAACACCATCTGCTTTGAGTCATGCATTAGATGTTGCT
    GATGAATTTGATGTTCAAATTGCATTACATGCAGATACTTTAAATGAAGCAGGATTTATG
    GAAGACACAATGGCTGCTGTTAAAGACCGTGTACTTCATATGTACCATACTGAAGGTGCT
    GGTGGCGGTCATGCGCCTGATTTAATTAAATCCGCTGCATTTTCAAATATTTTACCTTCA
    TCTACAAATCCAACTTTGCCTTATACACATAATACTGTAGATGAACATTTAGATATGGTA
    ATGATTACTCACCATTTAAATGCGGCTATTCCTGAAGATATCGCATTCGCAGATTCACGT
    ATTCGTAAAGAAACGATTGCAGCAGAAGATGTTCTGCAAGATATGGGTGTATTCAGTATG
    ATTAGTTCCGATTCACAAGCAATGGGCCGTGTAGGTGAAGTAATTACACGAACATGGCAA
    GTAGCACATCGCATGAAAGAACAACGTGGTCCTTTAGATGGTGATTTTGAACATAATGAT
    AATAATCGCATCAAACGTTATATCGCTAAATATACAATTAACCCAGCAATTACACATGGT
    ATTTCTGAATATGTAGGATCTATCGAGCCGGGCAAACTAGCTGACATTGTCTTATGGGAC
    CCAATTTTCTTTGGGGTTAAACCTGAATTAGTTGTAAAGGGCGGATTAATTAACTCTGCC
    GTAAATGGCGATGCAAATGGTTCTATACCTACATCTGAACCGATGAAGTACCGTAAAATG
    TATGGTCAATACGGCGGAAACCTTACAAGTACGTCAATGACATTCGTGTCTAAAACTGCT
    TATGAAAATGGTATCAATCGTGCATTAAATTTAAAACGCATGGTGCGTCCAGTTAAAAAT
    ATTAGACAATTATCTAAAGCAGATATGAAAAATAACAGTGCAACACCTAAATTAGACGTT
    GATCCACAAACATATGAAGTATATGTAGATGGAGAAAAAATTACAAGTAATGCAGCAACT
    GAGTTACCATTAACTCAAAGATACTTCTTATTCTAG
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1200
    1260
    1320
    1380
    1440
    1500
    1560
    1620
    1680
    1716

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: JSNZ_002256
  • symbol: UreC
  • description: urease subunit alpha
  • length: 571
  • theoretical pI: 5.06399
  • theoretical MW: 61779.2
  • GRAVY: -0.226445

⊟Function[edit | edit source]

  • reaction:
    EC 3.5.1.5?  ExPASy
    Urease Urea + H2O = CO2 + 2 NH3
  • TIGRFAM:
    Metabolism Central intermediary metabolism Nitrogen metabolism urease, alpha subunit (TIGR01792; EC 3.5.1.5; HMM-score: 1027.6)
    and 8 more
    Metabolism Purines, pyrimidines, nucleosides, and nucleotides Salvage of nucleosides and nucleotides adenine deaminase (TIGR01178; EC 3.5.4.2; HMM-score: 28)
    allantoinase (TIGR03178; EC 3.5.2.5; HMM-score: 27.9)
    dihydropyrimidinase (TIGR02033; EC 3.5.2.2; HMM-score: 25.4)
    Metabolism Purines, pyrimidines, nucleosides, and nucleotides Pyrimidine ribonucleotide biosynthesis dihydroorotase, multifunctional complex type (TIGR00857; EC 3.5.2.3; HMM-score: 24.3)
    Metabolism Energy metabolism Amino acids and amines imidazolonepropionase (TIGR01224; EC 3.5.2.7; HMM-score: 22.9)
    Metabolism Purines, pyrimidines, nucleosides, and nucleotides Other guanine deaminase (TIGR02967; EC 3.5.4.3; HMM-score: 18)
    Metabolism Energy metabolism Other phosphonate metabolism protein PhnM (TIGR02318; HMM-score: 16.3)
    formylmethanofuran dehydrogenase subunit A (TIGR03121; EC 1.2.99.5; HMM-score: 13)
  • TheSEED: data available for COL, N315, NCTC8325, Newman, USA300_FPR3757
  • PFAM:
    TIM_barrel (CL0036) Amidohydro_1; Amidohydrolase family (PF01979; HMM-score: 265.1)
    and 2 more
    Urease_alpha; Urease alpha-subunit, N-terminal domain (PF00449; HMM-score: 170.9)
    Amidohydro_3; Amidohydrolase family (PF07969; HMM-score: 43.8)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 9.97
    • Cytoplasmic Membrane Score: 0
    • Cellwall Score: 0.01
    • Extracellular Score: 0.02
    • Internal Helices: 0
  • DeepLocPro: Cytoplasmic
    • Cytoplasmic Score: 0.829
    • Cytoplasmic Membrane Score: 0.0167
    • Cell wall & surface Score: 0.0087
    • Extracellular Score: 0.1457
  • LocateP:
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.011924
    • TAT(Tat/SPI): 0.00069
    • LIPO(Sec/SPII): 0.002167
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

  • GI:
  • RefSeq:
  • UniProt:

⊟Protein sequence[edit | edit source]

  • MSFKMTQNQYTSLYGPTVGDSIRLGDTNLFAQIEKDYAVYGEEATFGGGKSIRDGMAQNPRVTRDDVNVADLVISNAVIIDYDKVVKADIGIKNGYIFAIGNAGNPDIMDNVDIIIGSTTDIIAAEGKIVTAGGIDTHVHFINPEQAEVALESGITTHIGGGTGASEGSKATTVTPGPWHIHRMLEAAEGLPINVGFTGKGQATNPTALIEQINAGAIGLKVHEDWGATPSALSHALDVADEFDVQIALHADTLNEAGFMEDTMAAVKDRVLHMYHTEGAGGGHAPDLIKSAAFSNILPSSTNPTLPYTHNTVDEHLDMVMITHHLNAAIPEDIAFADSRIRKETIAAEDVLQDMGVFSMISSDSQAMGRVGEVITRTWQVAHRMKEQRGPLDGDFEHNDNNRIKRYIAKYTINPAITHGISEYVGSIEPGKLADIVLWDPIFFGVKPELVVKGGLINSAVNGDANGSIPTSEPMKYRKMYGQYGGNLTSTSMTFVSKTAYENGINRALNLKRMVRPVKNIRQLSKADMKNNSATPKLDVDPQTYEVYVDGEKITSNAATELPLTQRYFLF

⊟Experimental data[edit | edit source]

  • experimentally validated: data available for COL, NCTC8325
  • protein localization: data available for COL
  • quantitative data / protein copy number per cell: data available for COL
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator:

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You can add further information about the gene and protein here. [edit]

⊟Literature[edit | edit source]

⊟References[edit | edit source]

  1. ↑ Blanca Taboada, Karel Estrada, Ricardo Ciria, Enrique Merino
    Operon-mapper: a web server for precise operon identification in bacterial and archaeal genomes.
    Bioinformatics: 2018, 34(23);4118-4120
    [PubMed:29931111] [WorldCat.org] [DOI] (I p)

⊟Relevant publications[edit | edit source]

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