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NCBI: 03-AUG-2016

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus NCTC8325
  • locus tag: SAOUHSC_02561
  • pan locus tag?: SAUPAN005755000
  • symbol: ureC
  • pan gene symbol?: ureC
  • synonym:
  • product: urease subunit alpha

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SAOUHSC_02561
  • symbol: ureC
  • product: urease subunit alpha
  • replicon: chromosome
  • strand: +
  • coordinates: 2352985..2354700
  • length: 1716
  • essential: no DEG other strains

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    ATGAGCTTTAAAATGACGCAAAATCAATATACGAGCTTATACGGTCCAACTGTTGGAGAT
    TCCATTCGTTTAGGTGATACGAATCTATTTGCTCAAATAGAAAAAGACTATGCGGTTTAT
    GGTGAAGAAGCTACTTTTGGTGGTGGTAAATCTATTAGAGACGGTATGGCGCAAAATCCT
    CGTGTAACACGTGATGACGTGAACGTTGCAGACCTTGTCATTTCTAATGCCGTTATTATC
    GATTACGATAAAGTGGTTAAAGCTGATATAGGCATTAAAAATGGTTATATTTTCGCCATA
    GGTAATGCCGGCAACCCAGATATAATGGATAATGTCGACATTATTATAGGTTCAACAACA
    GATATCATTGCCGCTGAAGGTAAAATCGTCACTGCTGGTGGTATTGATACTCATGTTCAT
    TTTATTAATCCTGAACAAGCAGAGGTCGCATTAGAAAGTGGTATTACGACTCATATTGGT
    GGTGGTACTGGTGCTTCAGAAGGTTCTAAAGCAACAACTGTAACTCCAGGTCCATGGCAT
    ATTCATAGAATGTTAGAAGCTGCCGAAGGTTTACCGATTAATGTCGGTTTTACAGGTAAA
    GGACAAGCAACAAATCCAACTGCACTCATTGAACAAATCAATGCCGGAGCAATTGGATTA
    AAAGTACATGAAGACTGGGGTGCAACACCATCTGCTTTGAGTCATGCATTAGATGTTGCT
    GATGAATTTGATGTTCAAATTGCATTACATGCAGATACTTTAAATGAAGCAGGATTTATG
    GAAGACACAATGGCTGCTGTTAAAGACCGTGTACTTCATATGTACCATACTGAAGGTGCT
    GGTGGCGGTCATGCGCCTGATTTAATTAAATCCGCTGCATTTTCAAATATTTTACCTTCA
    TCTACAAATCCAACTTTGCCTTATACACATAATACTGTAGATGAACATTTAGATATGGTA
    ATGATTACTCACCATTTAAATGCGGCTATTCCTGAAGATATCGCATTCGCAGATTCACGT
    ATTCGTAAAGAAACGATTGCAGCAGAAGATGTTCTGCAAGATATGGGTGTATTCAGTATG
    ATTAGTTCCGATTCACAAGCAATGGGCCGTGTAGGTGAAGTAATTACACGAACATGGCAA
    GTAGCACATCGCATGAAAGAACAACGTGGTCCTTTAGATGGTGATTTTGAACATAATGAT
    AATAATCGCATCAAACGTTATATCGCTAAATATACAATTAACCCAGCAATTACACATGGT
    ATTTCTGAATATGTAGGATCTATCGAGCCGGGCAAACTAGCTGACATTGTCTTATGGGAC
    CCAATTTTCTTTGGGGTTAAACCTGAATTAGTTGTAAAGGGCGGATTAATTAACTCTGCC
    GTAAATGGCGATGCAAATGGTTCTATACCTACATCTGAACCGATGAAGTACCGTAAAATG
    TATGGTCAATACGGCGGAAACCTTACAAGTACGTCAATGACATTCGTGTCTAAAACTGCT
    TATGAAAATGGTATCAATCGTGCATTAAATTTAAAACGCATGGTGCGTCCAGTTAAAAAT
    ATTAGACAATTATCTAAAGCAGATATGAAAAATAACAGTGCAACACCTAAATTAGACGTT
    GATCCACAAACATATGAAGTATATGTAGATGGAGAAAAAATTACAAGTAATGCAGCAACT
    GAGTTACCATTAACTCAAAGATACTTCTTATTCTAG
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1200
    1260
    1320
    1380
    1440
    1500
    1560
    1620
    1680
    1716

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SAOUHSC_02561
  • symbol: UreC
  • description: urease subunit alpha
  • length: 571
  • theoretical pI: 5.06399
  • theoretical MW: 61779.2
  • GRAVY: -0.226445

⊟Function[edit | edit source]

  • reaction:
    EC 3.5.1.5?  ExPASy
    Urease Urea + H2O = CO2 + 2 NH3
  • TIGRFAM:
    Metabolism Central intermediary metabolism Nitrogen metabolism urease, alpha subunit (TIGR01792; EC 3.5.1.5; HMM-score: 1027.6)
    and 8 more
    Metabolism Purines, pyrimidines, nucleosides, and nucleotides Salvage of nucleosides and nucleotides adenine deaminase (TIGR01178; EC 3.5.4.2; HMM-score: 28)
    allantoinase (TIGR03178; EC 3.5.2.5; HMM-score: 27.9)
    dihydropyrimidinase (TIGR02033; EC 3.5.2.2; HMM-score: 25.4)
    Metabolism Purines, pyrimidines, nucleosides, and nucleotides Pyrimidine ribonucleotide biosynthesis dihydroorotase, multifunctional complex type (TIGR00857; EC 3.5.2.3; HMM-score: 24.3)
    Metabolism Energy metabolism Amino acids and amines imidazolonepropionase (TIGR01224; EC 3.5.2.7; HMM-score: 22.9)
    Metabolism Purines, pyrimidines, nucleosides, and nucleotides Other guanine deaminase (TIGR02967; EC 3.5.4.3; HMM-score: 18)
    Metabolism Energy metabolism Other phosphonate metabolism protein PhnM (TIGR02318; HMM-score: 16.3)
    formylmethanofuran dehydrogenase subunit A (TIGR03121; EC 1.2.99.5; HMM-score: 13)
  • TheSEED  :
    • Urease alpha subunit (EC 3.5.1.5)
    Amino Acids and Derivatives Arginine; urea cycle, polyamines Urea decomposition  Urease alpha subunit (EC 3.5.1.5)
    and 1 more
    Amino Acids and Derivatives Arginine; urea cycle, polyamines Urease subunits  Urease alpha subunit (EC 3.5.1.5)
  • PFAM:
    Amidohydrolase (CL0034) Amidohydro_1; Amidohydrolase family (PF01979; HMM-score: 258.7)
    and 2 more
    Urease_alpha; Urease alpha-subunit, N-terminal domain (PF00449; HMM-score: 168.9)
    Amidohydro_3; Amidohydrolase family (PF07969; HMM-score: 44.3)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors: Ni cation
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 9.97
    • Cytoplasmic Membrane Score: 0
    • Cellwall Score: 0.01
    • Extracellular Score: 0.02
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular possibility: 1
    • Signal peptide possibility: -1
    • N-terminally Anchored Score: -1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.011924
    • TAT(Tat/SPI): 0.00069
    • LIPO(Sec/SPII): 0.002167
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MSFKMTQNQYTSLYGPTVGDSIRLGDTNLFAQIEKDYAVYGEEATFGGGKSIRDGMAQNPRVTRDDVNVADLVISNAVIIDYDKVVKADIGIKNGYIFAIGNAGNPDIMDNVDIIIGSTTDIIAAEGKIVTAGGIDTHVHFINPEQAEVALESGITTHIGGGTGASEGSKATTVTPGPWHIHRMLEAAEGLPINVGFTGKGQATNPTALIEQINAGAIGLKVHEDWGATPSALSHALDVADEFDVQIALHADTLNEAGFMEDTMAAVKDRVLHMYHTEGAGGGHAPDLIKSAAFSNILPSSTNPTLPYTHNTVDEHLDMVMITHHLNAAIPEDIAFADSRIRKETIAAEDVLQDMGVFSMISSDSQAMGRVGEVITRTWQVAHRMKEQRGPLDGDFEHNDNNRIKRYIAKYTINPAITHGISEYVGSIEPGKLADIVLWDPIFFGVKPELVVKGGLINSAVNGDANGSIPTSEPMKYRKMYGQYGGNLTSTSMTFVSKTAYENGINRALNLKRMVRPVKNIRQLSKADMKNNSATPKLDVDPQTYEVYVDGEKITSNAATELPLTQRYFLF

⊟Experimental data[edit | edit source]

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator:

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

  1. ↑ Maren Depke, Stephan Michalik, Alexander Rabe, Kristin Surmann, Lars Brinkmann, Nico Jehmlich, Jörg Bernhardt, Michael Hecker, Bernd Wollscheid, Zhi Sun, Robert L Moritz, Uwe Völker, Frank Schmidt
    A peptide resource for the analysis of Staphylococcus aureus in host-pathogen interaction studies.
    Proteomics: 2015, 15(21);3648-61
    [PubMed:26224020] [WorldCat.org] [DOI] (I p)
  2. ↑ Stephan Michalik, Maren Depke, Annette Murr, Manuela Gesell Salazar, Ulrike Kusebauch, Zhi Sun, Tanja C Meyer, Kristin Surmann, Henrike Pförtner, Petra Hildebrandt, Stefan Weiss, Laura Marcela Palma Medina, Melanie Gutjahr, Elke Hammer, Dörte Becher, Thomas Pribyl, Sven Hammerschmidt, Eric W Deutsch, Samuel L Bader, Michael Hecker, Robert L Moritz, Ulrike Mäder, Uwe Völker, Frank Schmidt
    A global Staphylococcus aureus proteome resource applied to the in vivo characterization of host-pathogen interactions.
    Sci Rep: 2017, 7(1);9718
    [PubMed:28887440] [WorldCat.org] [DOI] (I e)
  3. ↑ 3.00 3.01 3.02 3.03 3.04 3.05 3.06 3.07 3.08 3.09 3.10 3.11 3.12 3.13 3.14 3.15 3.16 3.17 3.18 3.19 3.20 3.21 3.22 3.23 3.24 3.25 3.26 3.27 3.28 3.29 3.30 3.31 3.32 3.33 3.34 3.35 3.36 3.37 3.38 3.39 3.40 3.41 3.42 3.43 3.44 3.45 3.46 3.47 3.48 3.49 3.50 3.51 3.52 3.53 3.54 3.55 3.56 3.57 3.58 3.59 3.60 3.61 3.62 3.63 3.64 3.65 3.66 3.67 3.68 3.69 3.70 3.71 3.72 3.73 3.74 3.75 3.76 3.77 Artem Cherkasov, Michael Hsing, Roya Zoraghi, Leonard J Foster, Raymond H See, Nikolay Stoynov, Jihong Jiang, Sukhbir Kaur, Tian Lian, Linda Jackson, Huansheng Gong, Rick Swayze, Emily Amandoron, Farhad Hormozdiari, Phuong Dao, Cenk Sahinalp, Osvaldo Santos-Filho, Peter Axerio-Cilies, Kendall Byler, William R McMaster, Robert C Brunham, B Brett Finlay, Neil E Reiner
    Mapping the protein interaction network in methicillin-resistant Staphylococcus aureus.
    J Proteome Res: 2011, 10(3);1139-50
    [PubMed:21166474] [WorldCat.org] [DOI] (I p)
  4. ↑ Ulrike Mäder, Pierre Nicolas, Maren Depke, Jan Pané-Farré, Michel Debarbouille, Magdalena M van der Kooi-Pol, Cyprien Guérin, Sandra Dérozier, Aurelia Hiron, Hanne Jarmer, Aurélie Leduc, Stephan Michalik, Ewoud Reilman, Marc Schaffer, Frank Schmidt, Philippe Bessières, Philippe Noirot, Michael Hecker, Tarek Msadek, Uwe Völker, Jan Maarten van Dijl
    Staphylococcus aureus Transcriptome Architecture: From Laboratory to Infection-Mimicking Conditions.
    PLoS Genet: 2016, 12(4);e1005962
    [PubMed:27035918] [WorldCat.org] [DOI] (I e)

⊟Relevant publications[edit | edit source]

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