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NCBI: 03-AUG-2016

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus NCTC8325
  • locus tag: SAOUHSC_02559
  • pan locus tag?: SAUPAN005754000
  • symbol: ureB
  • pan gene symbol?: ureB
  • synonym:
  • product: urease subunit beta

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SAOUHSC_02559
  • symbol: ureB
  • product: urease subunit beta
  • replicon: chromosome
  • strand: +
  • coordinates: 2352578..2352988
  • length: 411
  • essential: no DEG other strains

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    ATGATACCAGGAGAAATTATTACAAAAAGTACAGAGGTTGAAATTAATAACCATCATCCT
    GAAACAGTTATCGAAGTTGAAAATACAGGAGATCGACCAATTCAAGTGGGCTCACATTTT
    CATTTTTATGAAGCAAATGCAGCATTAGATTTCGAACGTGAAATGGCATATGGAAAACAT
    TTAGATATTCCAGCTGGAGCAGCTGTTCGATTTGAACCTGGGGATAAAAAAGAAGTTCAA
    TTAGTTGAATATGCTGGCAAACGTAAAATTTTTGGTTTTCGTGGTATGGTCAATGGTCCT
    ATCGATGAGTCACGTGTCTATCGCCCAACTGATGAAAATGATGAATATGCAGGTGTATTC
    GGAGATAACGGTGCTGAAAACGTGAATAAAAAAGGAGGAAAAAGATCATGA
    60
    120
    180
    240
    300
    360
    411

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SAOUHSC_02559
  • symbol: UreB
  • description: urease subunit beta
  • length: 136
  • theoretical pI: 5.05109
  • theoretical MW: 15164.7
  • GRAVY: -0.725

⊟Function[edit | edit source]

  • reaction:
    EC 3.5.1.5?  ExPASy
    Urease Urea + H2O = CO2 + 2 NH3
  • TIGRFAM:
    Metabolism Central intermediary metabolism Nitrogen metabolism urease, beta subunit (TIGR00192; EC 3.5.1.5; HMM-score: 150.2)
  • TheSEED  :
    • Urease beta subunit (EC 3.5.1.5)
    Amino Acids and Derivatives Arginine; urea cycle, polyamines Urea decomposition  Urease beta subunit (EC 3.5.1.5)
    and 1 more
    Amino Acids and Derivatives Arginine; urea cycle, polyamines Urease subunits  Urease beta subunit (EC 3.5.1.5)
  • PFAM:
    no clan defined Urease_beta; Urease beta subunit (PF00699; HMM-score: 146.9)
    and 1 more
    PapD-like (CL0556) PapD_N; Pili and flagellar-assembly chaperone, PapD N-terminal domain (PF00345; HMM-score: 12.2)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 9.97
    • Cytoplasmic Membrane Score: 0
    • Cellwall Score: 0.01
    • Extracellular Score: 0.02
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular possibility: 1
    • Signal peptide possibility: -1
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.004773
    • TAT(Tat/SPI): 0.000229
    • LIPO(Sec/SPII): 0.000375
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MIPGEIITKSTEVEINNHHPETVIEVENTGDRPIQVGSHFHFYEANAALDFEREMAYGKHLDIPAGAAVRFEPGDKKEVQLVEYAGKRKIFGFRGMVNGPIDESRVYRPTDENDEYAGVFGDNGAENVNKKGGKRS

⊟Experimental data[edit | edit source]

  • experimentally validated: data available for COL
  • protein localization: data available for COL
  • quantitative data / protein copy number per cell: data available for COL
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator:

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

  1. ↑ Ulrike Mäder, Pierre Nicolas, Maren Depke, Jan Pané-Farré, Michel Debarbouille, Magdalena M van der Kooi-Pol, Cyprien Guérin, Sandra Dérozier, Aurelia Hiron, Hanne Jarmer, Aurélie Leduc, Stephan Michalik, Ewoud Reilman, Marc Schaffer, Frank Schmidt, Philippe Bessières, Philippe Noirot, Michael Hecker, Tarek Msadek, Uwe Völker, Jan Maarten van Dijl
    Staphylococcus aureus Transcriptome Architecture: From Laboratory to Infection-Mimicking Conditions.
    PLoS Genet: 2016, 12(4);e1005962
    [PubMed:27035918] [WorldCat.org] [DOI] (I e)

⊟Relevant publications[edit | edit source]

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