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NCBI: 03-AUG-2016

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus NCTC8325
  • locus tag: SAOUHSC_02563
  • pan locus tag?: SAUPAN005757000
  • symbol: SAOUHSC_02563
  • pan gene symbol?: ureF
  • synonym:
  • product: urease accessory protein UreF

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SAOUHSC_02563
  • symbol: SAOUHSC_02563
  • product: urease accessory protein UreF
  • replicon: chromosome
  • strand: +
  • coordinates: 2355158..2355847
  • length: 690
  • essential: no DEG other strains

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    ATGATTGATCATACACACTTAAGATTATTTCAGTTCTGTGATTCACAGTTTCCAACAGGT
    GCTTTCAGTCATTCATTTGGTCTTGAAACATATATTCAACGAAATATAATTCATGACGAT
    CATACTTTTATTGCTTGGTTAAAAATGTTTTTACAAGAGCAACTTACCTATTCTGATGGT
    TTAGCCATGCGTTTAGTTTATGATGCATTGGAAAATGATGATACACAAAAAGTATTACAC
    ATAGATAAACTGATGTTTGTTCAAAACTTACCTAAAGAAACACGTGTTGGTGCAAAACAA
    ATGGGGACTCGCATGGTTAAATTGGCTTTAGAACTTTATAATAGCCCATGGATTGCTTGG
    TATCATCAACAAATGCAAGATAAGAAAGCAAAGTTAAATCCAGCTATTTGTTTTACTATG
    CTAGGCCATCATTTAGGTGTAGATATTGAAACGATTATTGATTACTATTTATATCAAAAT
    GTTTCAAGTTTAACTCAAAATGCTGTTCGTGCTATTCCACTTGGACAAACTGCTGGTCAA
    AAGATAGTTACTCACATGATTCCTTATATAGAAGGAACAAGAAAGCAGATTTTCGAATTA
    AAAGAAGCAGATTTTGGCATGACAGCGCCTGGTTTAGAACTAAATCAAATGGCGCATGAG
    AACGTCAATGTTAGAATTTTCATATCATAG
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    690

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SAOUHSC_02563
  • symbol: SAOUHSC_02563
  • description: urease accessory protein UreF
  • length: 229
  • theoretical pI: 6.74155
  • theoretical MW: 26479.4
  • GRAVY: -0.169869

⊟Function[edit | edit source]

  • TIGRFAM:
  • TheSEED  :
    • Urease accessory protein UreF
    Amino Acids and Derivatives Arginine; urea cycle, polyamines Urea decomposition  Urease accessory protein UreF
    and 1 more
    Amino Acids and Derivatives Arginine; urea cycle, polyamines Urease subunits  Urease accessory protein UreF
  • PFAM:
    no clan defined UreF; UreF (PF01730; HMM-score: 106.5)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 7.5
    • Cytoplasmic Membrane Score: 1.15
    • Cellwall Score: 0.62
    • Extracellular Score: 0.73
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular possibility: 1
    • Signal peptide possibility: -1
    • N-terminally Anchored Score: -1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.002033
    • TAT(Tat/SPI): 0.000167
    • LIPO(Sec/SPII): 0.000587
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MIDHTHLRLFQFCDSQFPTGAFSHSFGLETYIQRNIIHDDHTFIAWLKMFLQEQLTYSDGLAMRLVYDALENDDTQKVLHIDKLMFVQNLPKETRVGAKQMGTRMVKLALELYNSPWIAWYHQQMQDKKAKLNPAICFTMLGHHLGVDIETIIDYYLYQNVSSLTQNAVRAIPLGQTAGQKIVTHMIPYIEGTRKQIFELKEADFGMTAPGLELNQMAHENVNVRIFIS

⊟Experimental data[edit | edit source]

  • experimentally validated:
  • protein localization: data available for COL
  • quantitative data / protein copy number per cell:
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator:

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

  1. ↑ Ulrike Mäder, Pierre Nicolas, Maren Depke, Jan Pané-Farré, Michel Debarbouille, Magdalena M van der Kooi-Pol, Cyprien Guérin, Sandra Dérozier, Aurelia Hiron, Hanne Jarmer, Aurélie Leduc, Stephan Michalik, Ewoud Reilman, Marc Schaffer, Frank Schmidt, Philippe Bessières, Philippe Noirot, Michael Hecker, Tarek Msadek, Uwe Völker, Jan Maarten van Dijl
    Staphylococcus aureus Transcriptome Architecture: From Laboratory to Infection-Mimicking Conditions.
    PLoS Genet: 2016, 12(4);e1005962
    [PubMed:27035918] [WorldCat.org] [DOI] (I e)

⊟Relevant publications[edit | edit source]

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