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FunGene: 08-OCT-2024

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus JSNZ
  • locus tag: JSNZ_002375
  • pan locus tag?: SAUPAN005938000
  • symbol: adcA
  • pan gene symbol?: —
  • synonym:
  • product: zinc ABC transporter substrate-binding lipoprotein AdcA

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: JSNZ_002375
  • symbol: adcA
  • product: zinc ABC transporter substrate-binding lipoprotein AdcA
  • replicon: chromosome
  • strand: -
  • coordinates: 2375407..2376954
  • length: 1548
  • essential: unknown other strains

⊟Accession numbers[edit | edit source]

  • Gene ID:
  • RefSeq:
  • BioCyc:
  • MicrobesOnline:

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    ATGAAAAAGAAATTAGGTATGTTACTTCTTGTACCAGCCGTAACTTTATCATTAGCCGCA
    TGTGGGAATGATGATGGAAAAGATAAAGATGGCAAGGTAACAATTAAAACGACAGTTTAT
    CCATTGCAATCATTTGCAGAGCAAATTGGTGGAAAACACGTGAAGGTATCATCAATCTAT
    CCAGCAGGGACAGATTTACATAGCTATGAACCAACACAAAAAGATATATTAAGTGCAAGC
    AAGTCAGACTTGTTTATGTATACAGGGGATAATTTAGATCCGGTTGCTAAGAAAGTTGCA
    TCTACTATCAAAGATAAAGATAAAAAACTGTCTTTAGAGGATAAATTAGATAAAGCAAAG
    CTTTTAACTGATCAACACGAGCATGGTGAAGAGCATGAACATGAGGGGCATGATCATGAG
    AAAGAAGAACATCATCATCATGGTGGATATGATCCACACGTATGGTTAGATCCTAAAATT
    AACCAAACTTTCGCTAAAGAAATTAAAGATGAATTAGTGAAAAAAGATCCAAAACATAAA
    GATGACTATGAGAAAAACTACAAAAAATTAAACGACGATCTTAAGAAAATTGATAACGAT
    ATGAAGCAAGTTACAAAAGATAAGCAAGGTAATGCAGTATTTATTTCACATGAATCAATT
    GGATACTTAGCTGATCGTTATGGTTTTGTTCAAAAAGGTATTCAAAATATGAATGCTGAA
    GATCCATCACAAAAAGAATTAACTAAAATTGTTAAAGAAATTAGAGATAGCAATGCTAAA
    TATATTCTTTACGAAGATAATGTTGCGAATAAAGTGACTGAAACAATTCGTAAAGAAACA
    GATGCGAAGCCTTTAAAATTCTACAACATGGAGTCTTTAAATAAAGAACAACAGAAAAAA
    GATAATATTACGTATCAATCATTAATGAAATCGAATATTGAAAATATCGGTAAAGCTTTA
    GACAGTGGTGTTAAAGTGAAAGACGACAAAGCTGAAAGTAAACACGATAAAGCAATTTCT
    GATGGGTATTTTAAAGATGAGCAAGTTAAAGACCGTGAATTAAGCGATTATGCTGGTGAA
    TGGCAATCTGTTTACCCTTACTTAAAAGACGGTACGCTTGATGAAGTGATGGAACATAAA
    GCTGAAAATGATCCGAAGAAATCTGCTAAAGATTTAAAAGCTTATTATGACAAAGGATAT
    AAAACTGATATTACTAACATTGATATAAAAGGAAATGAAATTACATTTACTAAAGATGGT
    AAGAAACACACTGGTAAATATGAATACAATGGTAAGAAAACATTGAAATATCCTAAAGGT
    AACCGTGGCGTGAGATTTATGTTTAAATTGGTCGATGGTAATGATAAAGACTTACCGAAA
    TTCATCCAATTTAGCGATCACAACATTGCACCTAAAAAGGCAGAACACTTCCATATCTTT
    ATGGGTAATGATAATGACGCGTTATTAAAAGAAATGGATAACTGGCCAACATATTATCCT
    TCAAAATTAAATAAAGACCAAATCAAAGAAGAAATGTTAGCGCATTAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1200
    1260
    1320
    1380
    1440
    1500
    1548

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: JSNZ_002375
  • symbol: AdcA
  • description: zinc ABC transporter substrate-binding lipoprotein AdcA
  • length: 515
  • theoretical pI: 7.14567
  • theoretical MW: 59102.1
  • GRAVY: -1.04796

⊟Function[edit | edit source]

  • TIGRFAM:
    Metabolism Transport and binding proteins Unknown substrate anchored repeat ABC transporter, substrate-binding protein (TIGR03772; HMM-score: 86.3)
  • TheSEED: data available for COL, N315, NCTC8325, Newman, USA300_FPR3757
  • PFAM:
    Calycin (CL0116) ZinT; ZinT (YodA) periplasmic lipocalin-like zinc-recruitment (PF09223; HMM-score: 275.8)
    Chelatase (CL0043) ZnuA; Zinc-uptake complex component A periplasmic (PF01297; HMM-score: 239.6)
    and 6 more
    no clan defined DUF4971; Domain of unknown function (DUF4971) (PF16341; HMM-score: 16.9)
    LPD3; Large polyvalent protein-associated domain 3 (PF18798; HMM-score: 14.3)
    DUF3107; Protein of unknown function (DUF3107) (PF11305; HMM-score: 13.5)
    sPC4_like (CL0609) T4_y05I_C; y05I-like putative transcription factor C-terminal domain (PF24448; HMM-score: 9.8)
    no clan defined Hia_Tpr_ring_dom; Trimeric autotransporter adhesin, Trp ring domain (PF22414; HMM-score: 9.2)
    DUF4460; Domain of unknown function (DUF4460) (PF14687; HMM-score: 8.7)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic Membrane
    • Cytoplasmic Score: 0
    • Cytoplasmic Membrane Score: 9.68
    • Cellwall Score: 0.17
    • Extracellular Score: 0.16
    • Internal Helices: 0
  • DeepLocPro: Cytoplasmic Membrane
    • Cytoplasmic Score: 0.0007
    • Cytoplasmic Membrane Score: 0.8565
    • Cell wall & surface Score: 0.0731
    • Extracellular Score: 0.0697
  • LocateP:
  • SignalP: Signal peptide LIPO(Sec/SPII) length 20 aa
    • SP(Sec/SPI): 0.00062
    • TAT(Tat/SPI): 0.000068
    • LIPO(Sec/SPII): 0.999149
    • Cleavage Site: CS pos: 20-21. LAA-CG. Pr: 0.9997
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

  • GI:
  • RefSeq:
  • UniProt:

⊟Protein sequence[edit | edit source]

  • MKKKLGMLLLVPAVTLSLAACGNDDGKDKDGKVTIKTTVYPLQSFAEQIGGKHVKVSSIYPAGTDLHSYEPTQKDILSASKSDLFMYTGDNLDPVAKKVASTIKDKDKKLSLEDKLDKAKLLTDQHEHGEEHEHEGHDHEKEEHHHHGGYDPHVWLDPKINQTFAKEIKDELVKKDPKHKDDYEKNYKKLNDDLKKIDNDMKQVTKDKQGNAVFISHESIGYLADRYGFVQKGIQNMNAEDPSQKELTKIVKEIRDSNAKYILYEDNVANKVTETIRKETDAKPLKFYNMESLNKEQQKKDNITYQSLMKSNIENIGKALDSGVKVKDDKAESKHDKAISDGYFKDEQVKDRELSDYAGEWQSVYPYLKDGTLDEVMEHKAENDPKKSAKDLKAYYDKGYKTDITNIDIKGNEITFTKDGKKHTGKYEYNGKKTLKYPKGNRGVRFMFKLVDGNDKDLPKFIQFSDHNIAPKKAEHFHIFMGNDNDALLKEMDNWPTYYPSKLNKDQIKEEMLAH

⊟Experimental data[edit | edit source]

  • experimentally validated: data available for COL, NCTC8325
  • protein localization: data available for COL
  • quantitative data / protein copy number per cell:
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator: Zur* (repression) regulon
    Zur*(TF)important in Zinc homeostasis;  regulation predicted or transferred from N315 and NCTC 8325  [1]

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You can add further information about the gene and protein here. [edit]

⊟Literature[edit | edit source]

⊟References[edit | edit source]

  1. ↑ Hannes Wolfgramm, Larissa Milena Busch, Jöran Tebben, Henry Mehlan, Lisa Hagenau, Thomas Sura, Tilly Hoffmüller, Elisa Bludau, Manuela Gesell Salazar, Alexander Reder, Stephan Michalik, Leif Steil, Kristin Surmann, Ulrike Mäder, Silva Holtfreter, Uwe Völker
    Integrated genomic and proteomic analysis of the mouse-adapted Staphylococcus aureus strain JSNZ.
    Curr Res Microb Sci: 2025, 9;100489
    [PubMed:41146725] [WorldCat.org] [DOI] (I e)

⊟Relevant publications[edit | edit source]

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