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NCBI: 10-JUN-2013

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus USA300_FPR3757
  • locus tag: SAUSA300_2351 [new locus tag: SAUSA300_RS12980 ]
  • pan locus tag?: SAUPAN005938000
  • symbol: SAUSA300_2351
  • pan gene symbol?: —
  • synonym:
  • product: Zn-binding lipoprotein adcA-like protein

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SAUSA300_2351 [new locus tag: SAUSA300_RS12980 ]
  • symbol: SAUSA300_2351
  • product: Zn-binding lipoprotein adcA-like protein
  • replicon: chromosome
  • strand: -
  • coordinates: 2528237..2529787
  • length: 1551
  • essential: unknown other strains

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    ATGAAAAAGAAATTAGGTATGTTACTTCTTGTACCAGCCGTAACTTTATCATTAGCCGCA
    TGTGGGAATGATGATGGAAAAGATAAAGATGGCAAGGTAACAATTAAAACGACAGTTTAT
    CCATTGCAATCATTTGCAGAGCAAATTGGTGGAAAACACGTGAAGGTATCATCAATCTAT
    CCAGCAGGGACAGATTTACATAGCTATGAACCAACACAAAAAGATATATTAAGTGCAAGC
    AAGTCAGACTTGTTTATGTATACAGGGGATAATTTAGATCCGGTTGCTAAGAAAGTTGCA
    TCTACTATCAAAGATAAAGATAAAAAACTGTCTTTAGAGGATAAATTAGATAAAGCAAAG
    CTTTTAACTGATCAACACGAGCATGGTGAAGAGCATGAACATGAGGGACATGATCATGAG
    AAAGAAGAACATCATCATCATCATGGTGGATATGATCCACACGTATGGTTAGATCCTAAA
    ATTAACCAAACTTTCGCTAAAGAAATTAAAGATGAATTAGTGAAAAAAGATCCAAAACAT
    AAAGATGACTATGAGAAAAACTACAAAAAATTAAACGACGATCTTAAGAAAATTGATAAC
    GATATGAAGCAAGTTACAAAAGATAAGCAAGGTAATGCAGTATTCATTTCACATGAATCA
    ATTGGATACTTAGCTGATTGTTATGGTTTTGTTCAAAAAGGTATTCAAAACATGAATGCT
    GAAGATCCATCACAAAAAGAATTAACTAAAATTGTTAAAGAAATTAGAGATAGCAATGCT
    AAATATATTCTTTACGAAGATAATGTTGCGAATAAAGTGACTGAAACAATTCGTAAAGAA
    ACAGATGCGAAGCCTTTAAAATTCTACAACATGGAGTCTTTAAATAAAGAACAACAGAAA
    AAAGATAATATTACGTATCAATCATTAATGAAATCTAATATTGAAAATATCGGTAAAGCT
    TTAGACAGTGGTGTTAAAGTGAAAGACGACAAAGCTGAAAGTAAACATGACAAAGCAATT
    TCTGATGGGTATTTTAAAGATGAGCAAGTTAAAGACCGTGAATTAAGCGATTATGCTGGT
    GAATGGCAATCTGTTTACCCTTACTTAAAAGACGGTACGCTTGATGAAGTGATGGAACAT
    AAAGCTGAAAATGATCCGAAGAAATCTGCTAAAGATTTAAAAGCTTATTATGACAAAGGA
    TATAAAACTGATATTACTAACATTGATATAAAAGGAAATGAAATTACATTTACTAAAGAT
    GGTAAGAAACACACTGGTAAATATGAATACAATGGTAAGAAAACATTGAAATATCCTAAA
    GGTAACCGTGGCGTGAGATTTATGTTTAAATTGGTCGATGGTAATGATAAAGACTTACCG
    AAATTCATCCAATTTAGCGATCACAACATTGCACCTAAAAAGGCAGAACACTTCCATATC
    TTTATGGGTAATGATAATGACGCGTTATTAAAAGAAATGGATAACTGGCCAACATATTAT
    CCTTCAAAATTAAATAAAGACCAAATCAAAGAAGAAATGTTAGCGCATTAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1200
    1260
    1320
    1380
    1440
    1500
    1551

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SAUSA300_2351 [new locus tag: SAUSA300_RS12980 ]
  • symbol: SAUSA300_2351
  • description: Zn-binding lipoprotein adcA-like protein
  • length: 516
  • theoretical pI: 7.05056
  • theoretical MW: 59186.2
  • GRAVY: -1.03857

⊟Function[edit | edit source]

  • TIGRFAM:
    Metabolism Transport and binding proteins Unknown substrate anchored repeat ABC transporter, substrate-binding protein (TIGR03772; HMM-score: 87.1)
  • TheSEED  :
    • Metal-binding protein ZinT
    • Zinc ABC transporter, substrate-binding AdcA
  • PFAM:
    Calycin (CL0116) ZinT; ZinT (YodA) periplasmic lipocalin-like zinc-recruitment (PF09223; HMM-score: 267.9)
    Chelatase (CL0043) ZnuA; Zinc-uptake complex component A periplasmic (PF01297; HMM-score: 221.6)
    and 4 more
    no clan defined DUF4845; Domain of unknown function (DUF4845) (PF16137; HMM-score: 17.2)
    DUF4971; Domain of unknown function (DUF4971) (PF16341; HMM-score: 15.5)
    DUF3107; Protein of unknown function (DUF3107) (PF11305; HMM-score: 13.8)
    DUF4407; Domain of unknown function (DUF4407) (PF14362; HMM-score: 6.7)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic Membrane
    • Cytoplasmic Score: 0
    • Cytoplasmic Membrane Score: 9.68
    • Cellwall Score: 0.17
    • Extracellular Score: 0.16
    • Internal Helices: 0
  • LocateP: Lipid anchored
    • Prediction by SwissProt Classification: Extracellular
    • Pathway Prediction: Sec-(SPII)
    • Intracellular possibility: 0
    • Signal peptide possibility: 0.5
    • N-terminally Anchored Score: -1
    • Predicted Cleavage Site: LSLAACG
  • SignalP: Signal peptide LIPO(Sec/SPII) length 20 aa
    • SP(Sec/SPI): 0.00062
    • TAT(Tat/SPI): 0.000068
    • LIPO(Sec/SPII): 0.999149
    • Cleavage Site: CS pos: 20-21. LAA-CG. Pr: 0.9997
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MKKKLGMLLLVPAVTLSLAACGNDDGKDKDGKVTIKTTVYPLQSFAEQIGGKHVKVSSIYPAGTDLHSYEPTQKDILSASKSDLFMYTGDNLDPVAKKVASTIKDKDKKLSLEDKLDKAKLLTDQHEHGEEHEHEGHDHEKEEHHHHHGGYDPHVWLDPKINQTFAKEIKDELVKKDPKHKDDYEKNYKKLNDDLKKIDNDMKQVTKDKQGNAVFISHESIGYLADCYGFVQKGIQNMNAEDPSQKELTKIVKEIRDSNAKYILYEDNVANKVTETIRKETDAKPLKFYNMESLNKEQQKKDNITYQSLMKSNIENIGKALDSGVKVKDDKAESKHDKAISDGYFKDEQVKDRELSDYAGEWQSVYPYLKDGTLDEVMEHKAENDPKKSAKDLKAYYDKGYKTDITNIDIKGNEITFTKDGKKHTGKYEYNGKKTLKYPKGNRGVRFMFKLVDGNDKDLPKFIQFSDHNIAPKKAEHFHIFMGNDNDALLKEMDNWPTYYPSKLNKDQIKEEMLAH

⊟Experimental data[edit | edit source]

  • experimentally validated: data available for COL, NCTC8325
  • protein localization: data available for COL
  • quantitative data / protein copy number per cell:
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator: Zur* (repression) regulon
    Zur*(TF)important in Zinc homeostasis; RegPrecise 

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

⊟Relevant publications[edit | edit source]

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