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NCBI: 06-JUL-2013
⊟Summary[edit | edit source]
- organism: Staphylococcus aureus Newman
- locus tag: NWMN_0023 [new locus tag: NWMN_RS00130 ]
- pan locus tag?: SAUPAN000040000
- symbol: orfX
- pan gene symbol?: orfX
- synonym: rlmH
- product: rRNA large subunit methyltransferase
⊟Genome View[edit | edit source]
⊟Gene[edit | edit source]
⊟General[edit | edit source]
- type: CDS
- locus tag: NWMN_0023 [new locus tag: NWMN_RS00130 ]
- symbol: orfX
- product: rRNA large subunit methyltransferase
- replicon: chromosome
- strand: +
- coordinates: 33692..34171
- length: 480
- essential: unknown other strains
⊟Accession numbers[edit | edit source]
- Gene ID: 5329929 NCBI
- RefSeq: YP_001331058 NCBI
- BioCyc:
- MicrobesOnline: 3705554 MicrobesOnline
⊟Phenotype[edit | edit source]
Share your knowledge and add information here. [edit]
⊟DNA sequence[edit | edit source]
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421ATGAAAATCACCATTTTAGCTGTAGGGAAACTAAAAGAGAAATATTGGAAGCAAGCCATA
GCAGAATATGAAAAACGTTTAGGCCCATACACCAAGATAGACATCATAGAAGTTCCAGAC
GAAAAAGCACCAGAAAATATGAGTGACAAAGAAATTGAGCAAGTAAAAGAAAAAGAAGGC
CAACGAATACTAGCCAAAATCAAACCACAATCCACAGTCATTACATTAGAAATACAAGGA
AAGATGCTATCTTCCGAAGGATTGGCCCAAGAATTGAACCAACGCATGACCCAAGGGCAA
AGCGACTTTGTTTTCGTCATTGGCGGATCAAACGGCCTGCACAAGGACGTCTTACAACGC
AGTAACTACGCACTATCATTCAGCAAAATGACATTCCCACATCAAATGATGCGGGTTGTG
TTAATTGAACAAGTGTACAGAGCATTTAAGATTATGCGAGGAGAGGCGTATCATAAGTAA60
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⊟Protein[edit | edit source]
⊟General[edit | edit source]
- locus tag: NWMN_0023 [new locus tag: NWMN_RS00130 ]
- symbol: OrfX
- description: rRNA large subunit methyltransferase
- length: 159
- theoretical pI: 9.85885
- theoretical MW: 18306.2
- GRAVY: -0.461635
⊟Function[edit | edit source]
- reaction: EC 2.1.1.177? ExPASy23S rRNA (pseudouridine1915-N3)-methyltransferase S-adenosyl-L-methionine + pseudouridine1915 in 23S rRNA = S-adenosyl-L-homocysteine + N3-methylpseudouridine1915 in 23S rRNA
- TIGRFAM: Protein synthesis tRNA and rRNA base modification rRNA large subunit m3Psi methyltransferase RlmH (TIGR00246; EC 2.1.1.177; HMM-score: 186.6)
- TheSEED: data available for N315, NCTC8325, USA300_FPR3757
- PFAM: SPOUT (CL0098) SPOUT_MTase; Predicted SPOUT methyltransferase (PF02590; HMM-score: 206.9)and 5 moreMethyltrn_RNA_4; SAM-dependent RNA methyltransferase (PF09936; HMM-score: 16.3)no clan defined ExbD; Biopolymer transport protein ExbD/TolR (PF02472; HMM-score: 13.2)GT-A (CL0110) Glyco_tranf_2_3; Glycosyltransferase like family 2 (PF13641; HMM-score: 12.9)no clan defined SCAB-IgPH; Fused Ig-PH domain of plant-specific actin-binding protein (PF16709; HMM-score: 12.5)PDDEXK (CL0236) Vsr; DNA mismatch endonuclease Vsr (PF03852; HMM-score: 11.9)
⊟Structure, modifications & cofactors[edit | edit source]
- domains:
- modifications:
- cofactors:
- effectors:
⊟Localization[edit | edit source]
- PSORTb: Cytoplasmic
- Cytoplasmic Score: 7.5
- Cytoplasmic Membrane Score: 1.15
- Cellwall Score: 0.62
- Extracellular Score: 0.73
- Internal Helices: 0
- LocateP: Intracellular
- Prediction by SwissProt Classification: Cytoplasmic
- Pathway Prediction: No pathway
- Intracellular possibility: 1
- Signal peptide possibility: -1
- N-terminally Anchored Score: 1
- Predicted Cleavage Site: No CleavageSite
- SignalP: no predicted signal peptide
- SP(Sec/SPI): 0.008899
- TAT(Tat/SPI): 0.00034
- LIPO(Sec/SPII): 0.002259
- predicted transmembrane helices (TMHMM): 0
⊟Accession numbers[edit | edit source]
⊟Protein sequence[edit | edit source]
- MKITILAVGKLKEKYWKQAIAEYEKRLGPYTKIDIIEVPDEKAPENMSDKEIEQVKEKEGQRILAKIKPQSTVITLEIQGKMLSSEGLAQELNQRMTQGQSDFVFVIGGSNGLHKDVLQRSNYALSFSKMTFPHQMMRVVLIEQVYRAFKIMRGEAYHK
⊟Experimental data[edit | edit source]
- experimentally validated: data available for NCTC8325
- protein localization:
- quantitative data / protein copy number per cell:
- interaction partners:
⊟Expression & Regulation[edit | edit source]
⊟Operon[edit | edit source]
- MicrobesOnline: no polycistronic organisation predicted
⊟Regulation[edit | edit source]
- regulator:
⊟Transcription pattern[edit | edit source]
- S.aureus Expression Data Browser: data available for NCTC8325
⊟Protein synthesis (provided by Aureolib)[edit | edit source]
- Aureolib: no data available
⊟Protein stability[edit | edit source]
- half-life: no data available
⊟Biological Material[edit | edit source]
⊟Mutants[edit | edit source]
⊟Expression vector[edit | edit source]
⊟lacZ fusion[edit | edit source]
⊟GFP fusion[edit | edit source]
⊟two-hybrid system[edit | edit source]
⊟FLAG-tag construct[edit | edit source]
⊟Antibody[edit | edit source]
⊟Other Information[edit | edit source]
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