Jump to navigation
Jump to search
⊟Summary[edit | edit source]
- pan ID?: SAUPAN000040000
- symbol?: orfX
- synonym: rlmH
- description?: 23S rRNA (pseudouridine(1915)-N(3))-methyltransferase RlmH
- 23S rRNA (pseudouridine(1915)-N(3))-methyltransferase RlmH
- rRNA large subunit methyltransferase
- rRNA large subunit methyltransferase H
- alpha/beta knot family protein
- LSU m3Psi1915 methyltransferase RlmH
- LSU m3Psi1915 methyltransferase RlmH ybeA
- ribosomal RNA large subunit methyltransferase H
- rRNA large subunit m3Psi methyltransferase RlmH
descriptions from strain specific annotations:
- strand?: +
- coordinates?: 40139..40622
- synteny block?: BlockID0000370
- occurrence?: in 91% of 34 strains
rlmH (orfX) : ribosome large subunit methyltransferase H [1]
• A crystal structure is available : 1VH0
The 3' end of the rlmH coding sequence contains the canonical SCCmec attB site with the rlmH core sequence TATCATAA becoming a direct repeat found in attR1, attR2 and attL of MRSA strains. Ribosome large subunit methyltransferase H (alt: ribosome large subunit nucleobase methyltransferase H, 23S rRNA pseudouridine(1942) N-(3)-methyltransferase) attaches a methyl group to the pseudouridine nucleobase 3-position of residue 1942 in 23S ribosomal RNA (S. aureus numbering; pseudouridine 1915 by E. coli numbering).
- 23S rRNA : Ψ (1942) → m3Ψ (1942)
⊟Orthologs[edit | edit source]
04-02981:
SA2981_0024 (orfX)
08BA02176:
C248_0027
11819-97:
MS7_0026 (rlmH)
6850:
RSAU_000027 (rlmH)
71193:
ST398NM01_0027
ECT-R 2:
ECTR2_27
ED133:
—
ED98:
—
HO 5096 0412:
SAEMRSA15_00240
JH1:
SaurJH1_0024
JH9:
SaurJH9_0024
JKD6008:
SAA6008_00025
JKD6159:
SAA6159_00024
JSNZ:
JSNZ_000026 (rlmH)
LGA251:
SARLGA251_00240
M013:
M013TW_0027
MRSA252:
SAR0024
MSHR1132:
SAMSHR1132_00250
MSSA476:
SAS0024
Mu3:
SAHV_0024
Mu50:
SAV0024
MW2:
MW0024 (orfX)
RF122:
SAB0024
ST398:
SAPIG0027
T0131:
SAT0131_00027 (cmk)
TCH60:
HMPREF0772_10443
TW20:
SATW20_00250
USA300_TCH1516:
USA300HOU_0024 (orfX)
VC40:
SAVC_00120
⊟Genome Viewer[edit | edit source]
| N315 | |
| NCTC8325 | |
| Newman | |
| USA300_FPR3757 |
⊟Alignments[edit | edit source]
- alignment of orthologues: CLUSTAL format alignment by MAFFT L-INS-i (v7.307)
N315 MKITILAVGKLKEKYWKQAIAEYEKRLGPYTKIDIIEVPDEKAPENMSDKEIEQVKEKEG
NCTC8325 MKITILAVGKLKEKYWKQAIAEYEKRLGPYTKIDIIEVPDEKAPENMSDKEIEQVKEKEG
Newman MKITILAVGKLKEKYWKQAIAEYEKRLGPYTKIDIIEVPDEKAPENMSDKEIEQVKEKEG
USA300_FPR3757 MKITILAVGKLKEKYWKQAIAEYEKRLGPYTKIDIIEVPDEKAPENMSDKEIEQVKEKEG
************************************************************
N315 QRILAKIKPQSTVITLEIQGKMLSSEGLAQELNQRMTQGQSDFVFVIGGSNGLHKDVLQR
NCTC8325 QRILAKIKPQSTVITLEIQGKMLSSEGLAQELNQRMTQGQSDFVFVIGGSNGLHKDVLQR
Newman QRILAKIKPQSTVITLEIQGKMLSSEGLAQELNQRMTQGQSDFVFVIGGSNGLHKDVLQR
USA300_FPR3757 QRILAKIKPQSTVITLEIQGKMLSSEGLAQELNQRMTQGQSDFVFVIGGSNGLHKDVLQR
************************************************************
N315 SNYALSFSKMTFPHQMMRVVLIEQVYRAFKIMRGEAYHK
NCTC8325 SNYALSFSKMTFPHQMMRVVLIEQVYRAFKIMRGEAYHK
Newman SNYALSFSKMTFPHQMMRVVLIEQVYRAFKIMRGEAYHK
USA300_FPR3757 SNYALSFSKMTFPHQMMRVVLIEQVYRAFKIMRGEAYHK
***************************************
- ↑ Lei Wang, Martin Safo, Gordon L Archer
Characterization of DNA sequences required for the CcrAB-mediated integration of staphylococcal cassette chromosome mec, a Staphylococcus aureus genomic island.
J Bacteriol: 2012, 194(2);486-98
[PubMed:22056931] [WorldCat.org] [DOI] (I p)Sam Boundy, Martin K Safo, Lei Wang, Faik N Musayev, Heather C O'Farrell, Jason P Rife, Gordon L Archer
Characterization of the Staphylococcus aureus rRNA methyltransferase encoded by orfX, the gene containing the staphylococcal chromosome Cassette mec (SCCmec) insertion site.
J Biol Chem: 2013, 288(1);132-40
[PubMed:23150671] [WorldCat.org] [DOI] (I p)Cha San Koh, Rohini Madireddy, Timothy J Beane, Phillip D Zamore, Andrei A Korostelev
Small methyltransferase RlmH assembles a composite active site to methylate a ribosomal pseudouridine.
Sci Rep: 2017, 7(1);969
[PubMed:28428565] [WorldCat.org] [DOI] (I e)Alexander Golubev, Bulat Fatkhullin, Iskander Khusainov, Lasse Jenner, Azat Gabdulkhakov, Shamil Validov, Gulnara Yusupova, Marat Yusupov, Konstantin Usachev
Cryo-EM structure of the ribosome functional complex of the human pathogen Staphylococcus aureus at 3.2 Å resolution.
FEBS Lett: 2020, 594(21);3551-3567
[PubMed:32852796] [WorldCat.org] [DOI] (I p)Adrián González-López, Daniel S D Larsson, Ravi Kiran Koripella, Brett N Cain, Martin Garcia Chavez, Paul J Hergenrother, Suparna Sanyal, Maria Selmer
Structures of the Staphylococcus aureus ribosome inhibited by fusidic acid and fusidic acid cyclopentane.
Sci Rep: 2024, 14(1);14253
[PubMed:38902339] [WorldCat.org] [DOI] (I e)