PangenomeCOLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 06-JUL-2013

⊟Summary

• organism: Staphylococcus aureus Newman
• locus tag: NWMN_0087 [new locus tag: NWMN_RS00495 ]
• pan locus tag^?: SAUPAN000958000
• symbol: NWMN_0087
• pan gene symbol^?: —
• synonym:
• product: hypothetical protein

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: NWMN_0087 [new locus tag: NWMN_RS00495 ]
• symbol: NWMN_0087
• product: hypothetical protein
• replicon: chromosome
• strand: +
• coordinates: 110270..111814
• length: 1545
• essential: unknown other strains

⊟Accession numbers

• Gene ID: 5329974 NCBI
• RefSeq: YP_001331122 NCBI
• BioCyc:
• MicrobesOnline: 3705618 MicrobesOnline

⊟Phenotype

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⊟DNA sequence

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  ATGAAAAAAATATATAAGTCATTAACTGTCTCTGCAATTGTTGCAACGGTATCATTAAGT
  GCTTTACCGCAATCTTTAGCTATAACGCATGAATCGCAACCTACAAAGCAACAGCGAACG
  GTATTATTCGATCGTTCTCATGGTCAAACAGCTGGTGCTGCAGATTGGGTTAGTGATGGT
  GCATTTTCAGATTATGCGGATTCAATACAAAAACAAGGTTATGACGTTAAAGCTATTGAT
  GGTCATTCGAACATAACAGAAGCAAGTTTGAAAAGTTCCAAAATATTTGTAATTCCTGAG
  GCTAACATTCCTTTCAAAGAATCAGAACAGGCAGCAATTGTTAAATATGTGAAACAAGGT
  GGCAATGTTGTCTTTATTTCAGATCATTACAATGCTGACCGAAATTTAAATCGTATTGAT
  TCATCGGAGGCAATGAATGGTTATCGACGTGGAGCATATGAAGATATGTCGAAAGGTATG
  AATGCAGAAGAAAAAAGTTCTACTGCAATGCAAGGTGTGAAAAGTTCAGATTGGTTATCT
  ACAAACTTTGGCGTACGTTTTCGATATAATGCACTAGGTGATTTAAATACGAGCAATATT
  GTTTCTTCAAAAGAAAGTTTCGGTATTACTGAAGGTGTGAAATCTGTCTCTATGCATGCC
  GGATCGACATTAGCAATTACTAATCCAGAGAAAGCAAAAGGTATTGTGTATACACCAGAA
  CAATTGCCAGCGAAAAGTAAATGGTCACATGCTGTAGATCAAGGTATTTATAATGGGGGC
  GGTAAAGCAGAAGGCCCCTATGTAGCAATTTCTAAAGTTGGAAAAGGTAAAGCAGCATTT
  ATCGGTGATTCATCACTTGTGGAAGATAGTTCGCCCAAATATGTAAGAGAAGATAATGGA
  GAAAAGAAGAAAACATATGATGGTTTTAAAGAACAAGACAACGGTAAGCTATTAAATAAT
  ATAACGGCTTGGATGTCTAAAGATAATGATGGGAAATCACTTAAGGCGAGTAGCCTAACA
  TTAGATACAAAGACTAAGTTGCTTGATTTTGAACGACCAGAGCGTTCAACTGAGCCTGAA
  AAAGAGCCATGGTCACAACCGCCGAGTGGTTATAAATGGTATGATCCAACAACATTTAAA
  GCAGGTAGTTATGGCAGCGAAAAAGGCGCAGATCCTCAGCCAAACACACCAGATGATCAT
  ACACCACCAAATCAGAACGAAAAAGTAACATTTGATATCCCGCAAAATGTTTCTGTAAAT
  GAGCCATTTGAAATGACAATACATTTAAAAGGATTTGAAGCAAATCAAACACTTGAAAAT
  CTTAGAGTTGGTATTTACAAAGAAGGCGGACGTCAAATCGGACAATTTTCAAGTAAAGAT
  AACGATTATAACCCACCAGGTTACAGTACTTTGCCAACAGTTAAAGCAGATGAAAACGGA
  AATGTCACAATTAAGGTCAATGCTAAAGTACTTGAAAGTATGGAAGGTTCAAAGATTCGT
  TTAAAACTCGGTGACAAAACCTTGATTACAACAGACTTCAAATAA
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⊟Protein

⊟General

• locus tag: NWMN_0087 [new locus tag: NWMN_RS00495 ]
• symbol: NWMN_0087
• description: hypothetical protein
• length: 514
• theoretical pI: 6.9843
• theoretical MW: 56301.3
• GRAVY: -0.704475

⊟Function

• TIGRFAM:
  gliding-associated putative ABC transporter substrate-binding component GldG (TIGR03521; HMM-score: 15.3)
• TheSEED: data available for COL, N315, NCTC8325, USA300_FPR3757
• PFAM:
  no clan defined ABC_transp_aux; ABC-type uncharacterized transport system (PF09822; HMM-score: 30.9)
  and 3 more
  Glutaminase_I (CL0014) ThuA; Trehalose utilisation (PF06283; HMM-score: 22.1)
  no clan defined SOXp; SOX transcription factor (PF12336; HMM-score: 16.5)
  DUF4350; Domain of unknown function (DUF4350) (PF14258; HMM-score: 12.7)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors:
• effectors:

⊟Localization

• PSORTb: unknown (no significant prediction)
  • Cytoplasmic Score: 0
  • Cytoplasmic Membrane Score: 3.33
  • Cellwall Score: 3.33
  • Extracellular Score: 3.33
  • Internal Helices: 0
• LocateP: Secretory(released) (with CS)
  • Prediction by SwissProt Classification: Extracellular
  • Pathway Prediction: Sec-(SPI)
  • Intracellular possibility: -0.17
  • Signal peptide possibility: 1
  • N-terminally Anchored Score: -1
  • Predicted Cleavage Site: PQSLAITH
• SignalP: Signal peptide SP(Sec/SPI) length 27 aa
  • SP(Sec/SPI): 0.987636
  • TAT(Tat/SPI): 0.004184
  • LIPO(Sec/SPII): 0.005656
  • Cleavage Site: CS pos: 27-28. SLA-IT. Pr: 0.8530
• predicted transmembrane helices (TMHMM): 0

⊟Accession numbers

• GI: 151220299 NCBI
• RefSeq: YP_001331122 NCBI
• UniProt: A0A0H3KA11 UniProt

⊟Protein sequence

• MKKIYKSLTVSAIVATVSLSALPQSLAITHESQPTKQQRTVLFDRSHGQTAGAADWVSDGAFSDYADSIQKQGYDVKAIDGHSNITEASLKSSKIFVIPEANIPFKESEQAAIVKYVKQGGNVVFISDHYNADRNLNRIDSSEAMNGYRRGAYEDMSKGMNAEEKSSTAMQGVKSSDWLSTNFGVRFRYNALGDLNTSNIVSSKESFGITEGVKSVSMHAGSTLAITNPEKAKGIVYTPEQLPAKSKWSHAVDQGIYNGGGKAEGPYVAISKVGKGKAAFIGDSSLVEDSSPKYVREDNGEKKKTYDGFKEQDNGKLLNNITAWMSKDNDGKSLKASSLTLDTKTKLLDFERPERSTEPEKEPWSQPPSGYKWYDPTTFKAGSYGSEKGADPQPNTPDDHTPPNQNEKVTFDIPQNVSVNEPFEMTIHLKGFEANQTLENLRVGIYKEGGRQIGQFSSKDNDYNPPGYSTLPTVKADENGNVTIKVNAKVLESMEGSKIRLKLGDKTLITTDFK

⊟Experimental data

• experimentally validated: data available for COL, NCTC8325
• protein localization: data available for COL
• quantitative data / protein copy number per cell:
• interaction partners:

⊟Expression & Regulation

⊟Operon

• MicrobesOnline: NWMN_0087 > NWMN_0088

⊟Regulation

• regulator:

⊟Transcription pattern

• S.aureus Expression Data Browser: data available for NCTC8325

⊟Protein synthesis (provided by Aureolib)

• Aureolib: no data available

⊟Protein stability

• half-life: no data available

⊟Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊟Other Information

You are kindly invited to share additional interesting facts.

⊟Literature

⊟References

⊟Relevant publications