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NCBI: 06-JUL-2013

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus Newman
  • locus tag: NWMN_0114 [new locus tag: NWMN_RS00630 ]
  • pan locus tag?: SAUPAN001005000
  • symbol: NWMN_0114
  • pan gene symbol?: —
  • synonym:
  • product: cation efflux family protein

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: NWMN_0114 [new locus tag: NWMN_RS00630 ]
  • symbol: NWMN_0114
  • product: cation efflux family protein
  • replicon: chromosome
  • strand: +
  • coordinates: 139273..140337
  • length: 1065
  • essential: unknown other strains

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    ATGGTTAATTACGGAAGCATTAATATTAACCTGAGAAGCTATAAAGAATTATTTTTAAAA
    GCGACAATATTAAATACGACGCATTTATTTAGGAGTGGCAAACGTATGAATGGGAAAAAG
    GCGAATACGATAAACAGATACAAATATTTTCATCATGTCAATCATCAAAAAATTCAACAA
    AGTTCTAAAAAGACGCTGTGGGCATCACTAATCATCACATTGTTATTTACAGTGATTGAA
    TTTGTCGGAGGTTTAGTATCTAATTCATTGGCATTACTGTCAGATTCATTTCATATGCTT
    AGTGATGTATTAGCACTTGGTTTATCTATGTTGGCCATTTATTTTGCAAGTAAAAAGCCG
    ACTGCACGATACACATTTGGATATTTAAGATTTGAGATATTAGCTGCATTTTTAAATGGT
    TTAGCATTAATTGTAATTTCAATCTGGATTTTATATGAAGCTATTGTACGTATTATTTAT
    CCGCAACCAATTGAAAGTGGCATTATGTTTATGATTGCTAGTATTGGTTTACTCGTCAAT
    ATTATTTTGACTGTTATCCTTGTAAGGTCTTTAAAACAAGAAGACAATATCAATATTCAA
    AGTGCATTATGGCATTTCATGGGAGACTTATTGAACTCTATTGGTGTCATCGTTGCAGTT
    GTATTGATTTACTTTACAGGATGGCGCATCATCGACCCAATCATTAGTATTGTAATTTCA
    CTCATCATTTTACGTGGTGGTTATAAAATTACGCGTAATGCGTGGTTAATTTTAATGGAA
    AGTGTGCCTCAACATTTGGATACTGATCAAATTATGGCAGATATTAAAAACATAGATGGC
    ATATTAGATGTACATGAATTTCATTTGTGGAGTATTACAACAGAGCATTATTCATTAAGT
    GCCCATGTTGTGTTAGATAAAAAATATGAGGGTGATGATTATCAAGCGATTGATCAAGTA
    TCATCATTGTTGAAAGAAAAATATGGCATTGCACATTCAACGTTGCAAATTGAAAACTTG
    CAATTGAATCCATTAGATGAGCCATACTTCGACAAATTAACATAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1065

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: NWMN_0114 [new locus tag: NWMN_RS00630 ]
  • symbol: NWMN_0114
  • description: cation efflux family protein
  • length: 354
  • theoretical pI: 8.59269
  • theoretical MW: 40275
  • GRAVY: 0.377401

⊟Function[edit | edit source]

  • TIGRFAM:
    Metabolism Transport and binding proteins Cations and iron carrying compounds cation diffusion facilitator family transporter (TIGR01297; HMM-score: 307.1)
    and 1 more
    Cellular processes Cellular processes Sporulation and germination stage III sporulation protein AC (TIGR02848; HMM-score: 10)
  • TheSEED: data available for COL, N315, NCTC8325, USA300_FPR3757
  • PFAM:
    no clan defined Cation_efflux; Cation efflux family (PF01545; HMM-score: 174.7)
    and 3 more
    DUF2663; Protein of unknown function (DUF2663) (PF10864; HMM-score: 12.9)
    Transporter (CL0375) SUR7; SUR7/PalI family (PF06687; HMM-score: 8.9)
    no clan defined DUF2070; Predicted membrane protein (DUF2070) (PF09843; HMM-score: 8.3)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic Membrane
    • Cytoplasmic Score: 0
    • Cytoplasmic Membrane Score: 10
    • Cellwall Score: 0
    • Extracellular Score: 0
    • Internal Helices: 6
  • LocateP: Multi-transmembrane
    • Prediction by SwissProt Classification: Membrane
    • Pathway Prediction: Sec-(SPI)
    • Intracellular possibility: 0.17
    • Signal peptide possibility: -1
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.010202
    • TAT(Tat/SPI): 0.010639
    • LIPO(Sec/SPII): 0.001175
  • predicted transmembrane helices (TMHMM): 6

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MVNYGSININLRSYKELFLKATILNTTHLFRSGKRMNGKKANTINRYKYFHHVNHQKIQQSSKKTLWASLIITLLFTVIEFVGGLVSNSLALLSDSFHMLSDVLALGLSMLAIYFASKKPTARYTFGYLRFEILAAFLNGLALIVISIWILYEAIVRIIYPQPIESGIMFMIASIGLLVNIILTVILVRSLKQEDNINIQSALWHFMGDLLNSIGVIVAVVLIYFTGWRIIDPIISIVISLIILRGGYKITRNAWLILMESVPQHLDTDQIMADIKNIDGILDVHEFHLWSITTEHYSLSAHVVLDKKYEGDDYQAIDQVSSLLKEKYGIAHSTLQIENLQLNPLDEPYFDKLT

⊟Experimental data[edit | edit source]

  • experimentally validated: data available for COL, NCTC8325
  • protein localization: data available for COL
  • quantitative data / protein copy number per cell:
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator: SigB (activation) regulon
    SigB(sigma factor)controlling a large regulon involved in stress/starvation response and adaptation;  [1]   other strains

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

  1. ↑ Markus Bischoff, Paul Dunman, Jan Kormanec, Daphne Macapagal, Ellen Murphy, William Mounts, Brigitte Berger-Bächi, Steven Projan
    Microarray-based analysis of the Staphylococcus aureus sigmaB regulon.
    J Bacteriol: 2004, 186(13);4085-99
    [PubMed:15205410] [WorldCat.org] [DOI] (P p)

⊟Relevant publications[edit | edit source]

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