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NCBI: 06-JUL-2013

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus Newman
  • locus tag: NWMN_0172 [new locus tag: NWMN_RS00950 ]
  • pan locus tag?: SAUPAN001098000
  • symbol: NWMN_0172
  • pan gene symbol?: prsS
  • synonym:
  • product: hypothetical protein

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: NWMN_0172 [new locus tag: NWMN_RS00950 ]
  • symbol: NWMN_0172
  • product: hypothetical protein
  • replicon: chromosome
  • strand: +
  • coordinates: 222650..223897
  • length: 1248
  • essential: unknown other strains

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    GTGAAAGTAGGTAAGTCTATGGAAGGTCTTAATCATCGAAGAAATACAGAAAAAGAAGAG
    ACAACACAAACGCAATCAGTTGCACCTAATACAGGTGAAGAGGGGATGTCATCAGCAAGT
    ACACAATCAACTAAGACGTCCGACATACATAATGAATCTATCGATAAACAAATGGAAGCT
    AAAGCGCATGAAACAGCGCAAAATACAGATTTAAAAAACGAAGCAAGAAGTTTATTTGAT
    AATGCAACCAAATCAATCGGTAGACTAGCGGGCAATGATGAAAGCTTAAATCTTAATTTA
    AAAGATATGCTTTCTGAAGTATTTAAGCCGCATACTAAAAACGAAGCAGATGAAATATTT
    ATAGCGGGTACTGCTAAAACTACGCCAGCAATTTGTGACATATCAGAAGAATGGGGGAAG
    CCATGGCTCTTTTCTCGAGTATTCATCGCTTTCACAGTAACATTTATTGGATTATGGGTC
    ATGGCAGCAATTTTTAATAACACTAACGCGATTCCGGGTCTCATTTTTATAGGGGCTTTA
    ACAGTACCATTATCGGGTTTGTTCTTCTTTTATGAATCAAATGCGTTTAAAAATATTAGC
    ATTTTTGAAGTTATTATCATGTTCTTTATTGGCGGCGTATTTTCATTACTAAGTACGATG
    GTATTATATAGATTTGTCGTTTTTAGTGATCAATTCGAAAGGTTTGGTTCTTTAACATTT
    TTCGATGCATTTTTAGTAGGATTAGTTGAAGAAACTGGAAAAGCACTCATTATTGTTTAT
    TTCGTCAATAAATTGAAAACAAATAAGATTTTGAATGGATTATTAATCGGTGCTGCTATT
    GGTGCAGGGTTCGCAGTTTTTGAATCAGCAGGTTATATTTTGAATTTCGCTTTAGGAGAA
    AATGTCCCATTATTAGATATTGTCTTCACACGTGCGTGGACTGCGATTGGTGGTCATTTA
    GTTTGGTCAGCGATTGTTGGTGCTGCAATAGTTATTGCGAAAGAACAGCATGGCTTTGAA
    TTCAAAGATATTTTTGATAAACGCTTTTTAATATTCTTTTTATCAGCCGTTGTTTTACAT
    GGCATTTGGGATACATCTTTAACTGTACTTGGCAGTGATACGTTGAAAATATTTATTTTA
    ATCGTTATTGTGTGGATACTTGTATTCATTTTAATGGGGGCAGGTTTAAAACAAGTGAAT
    TTACTGCAGAAAGAATTTAAAGAACAACAGAAAAAAGTAGACGAATAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1200
    1248

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: NWMN_0172 [new locus tag: NWMN_RS00950 ]
  • symbol: NWMN_0172
  • description: hypothetical protein
  • length: 415
  • theoretical pI: 5.46553
  • theoretical MW: 46040
  • GRAVY: 0.314699

⊟Function[edit | edit source]

  • TIGRFAM:
  • TheSEED: data available for COL, N315, NCTC8325, USA300_FPR3757
  • PFAM:
    Peptidase_U (CL0472) PrsW-protease; Protease prsW family (PF13367; HMM-score: 93.2)
    and 2 more
    Sm-like (CL0527) LSM; LSM domain (PF01423; HMM-score: 11.8)
    no clan defined MWFE; NADH-ubiquinone oxidoreductase MWFE subunit (PF15879; HMM-score: 8.8)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic Membrane
    • Cytoplasmic Score: 0
    • Cytoplasmic Membrane Score: 10
    • Cellwall Score: 0
    • Extracellular Score: 0
    • Internal Helices: 8
  • LocateP: Multi-transmembrane
    • Prediction by SwissProt Classification: Membrane
    • Pathway Prediction: Sec-(SPI)
    • Intracellular possibility: 0.17
    • Signal peptide possibility: -1
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.030803
    • TAT(Tat/SPI): 0.00982
    • LIPO(Sec/SPII): 0.008437
  • predicted transmembrane helices (TMHMM): 8

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MKVGKSMEGLNHRRNTEKEETTQTQSVAPNTGEEGMSSASTQSTKTSDIHNESIDKQMEAKAHETAQNTDLKNEARSLFDNATKSIGRLAGNDESLNLNLKDMLSEVFKPHTKNEADEIFIAGTAKTTPAICDISEEWGKPWLFSRVFIAFTVTFIGLWVMAAIFNNTNAIPGLIFIGALTVPLSGLFFFYESNAFKNISIFEVIIMFFIGGVFSLLSTMVLYRFVVFSDQFERFGSLTFFDAFLVGLVEETGKALIIVYFVNKLKTNKILNGLLIGAAIGAGFAVFESAGYILNFALGENVPLLDIVFTRAWTAIGGHLVWSAIVGAAIVIAKEQHGFEFKDIFDKRFLIFFLSAVVLHGIWDTSLTVLGSDTLKIFILIVIVWILVFILMGAGLKQVNLLQKEFKEQQKKVDE

⊟Experimental data[edit | edit source]

  • experimentally validated: data available for COL, NCTC8325
  • protein localization: data available for COL
  • quantitative data / protein copy number per cell:
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator:

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

⊟Relevant publications[edit | edit source]

Christina N Krute, Harris Bell-Temin, Halie K Miller, Frances E Rivera, Andy Weiss, Stanley M Stevens, Lindsey N Shaw
The membrane protein PrsS mimics σS in protecting Staphylococcus aureus against cell wall-targeting antibiotics and DNA-damaging agents.
Microbiology (Reading): 2015, 161(Pt 5);1136-1148
[PubMed:25741016] [WorldCat.org] [DOI] (I p)

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