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NCBI: 26-AUG-2013

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus N315
  • locus tag: SA0228 [new locus tag: SA_RS01335 ]
  • pan locus tag?: SAUPAN001098000
  • symbol: SA0228
  • pan gene symbol?: prsS
  • synonym:
  • product: hypothetical protein

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SA0228 [new locus tag: SA_RS01335 ]
  • symbol: SA0228
  • product: hypothetical protein
  • replicon: chromosome
  • strand: +
  • coordinates: 275772..276914
  • length: 1143
  • essential: no DEG other strains

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    ATGTCATCAGCAAGTACACAATCAACTAAGACGTCCGACATACATAATGAATCTATCAAT
    AAACAAATGGAAGCCAAAGCGCATGAAACAGCGCAAAATGCAGATTTAAAAACCGAAGCA
    AGAAGTTTATTTGATAATGCAACCAAATCAATCGGTAGACTAGCCGGCAATGATGAAAGC
    TTAAATCTTAATTTAAAAGATATGTTTTCTGAAGTATTTAAGCCGCATACTAAAAACGAA
    GCAGATGAAATATTTATAGCGGGTACTGCTAAAACTACGCCAGCAATTTGTGACATATCA
    GAAGAATGGGGGAAGCCATGGCTCTTTTCTCGAGTATTCATCGCTTTCACAGTAACATTT
    ATTGGATTATGGGTCATGGCAGCGATTTTTAATAACACTAACGCGATTCCGGGTCTCATT
    TTTATAGGGGCTTTAACAGTACCATTATCGGGTTTGTTCTTCTTTTATGAATCAAATGCG
    TTTAAAAATATTAGCATTTTTGAAGTTATTATCATGTTCTTTATTGGCGGCGTATTTTCA
    TTACTAAGTACGATGGTATTATATAGATTTGTCGTTTTTAGTGATCAATTCGAAAGGTTT
    GGTTCTTTAACATTTTTCGATGCATTTTTAGTAGGATTAGTTGAAGAAACTGGAAAAGCA
    CTCATTATTGTTTATTTCGTCAATAAATTGAAAACAAATAAGATTTTGAATGGATTATTA
    ATCGGTGCTGCTATTGGTGCAGGGTTCGCAGTTTTTGAATCAGCAGGTTATATTTTGAAT
    TTCGCTTTAGGGGAAAATGTCCCATTATTAGATATTGTCTTCACACGTGCGTGGACTGCG
    ATTGGTGGTCATTTAGTTTGGTCAGCGATTGTTGGTGCTGCAATAGTTATTGCGAAAGAA
    CAGCATGGCTTTGAATTCAAAGATATTTTTGATAAACGCTTTTTAATATTCTTTTTATCA
    GCCGTTGGTTTACATGGCATTTGGGATACATCTTTAACTGTACTTGGCAGTGATACGTTG
    AAAATATTTATTTTAATCGTTATTGTGTGGATACTTGTATTCATTTTAATGGGGGCAGGT
    TTAAAACAAGTGAATTTACTGCAGAAAGAATTTAAAGAACAACAGAAAAAAGTAGACGAA
    TAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1143

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SA0228 [new locus tag: SA_RS01335 ]
  • symbol: SA0228
  • description: hypothetical protein
  • length: 380
  • theoretical pI: 5.68735
  • theoretical MW: 42130.7
  • GRAVY: 0.482105

⊟Function[edit | edit source]

  • TIGRFAM:
  • TheSEED  :
    • FIG01108038: hypothetical protein
  • PFAM:
    Peptidase_U (CL0472) PrsW-protease; Protease prsW family (PF13367; HMM-score: 92.4)
    and 2 more
    Sm-like (CL0527) LSM; LSM domain (PF01423; HMM-score: 13.4)
    no clan defined MWFE; NADH-ubiquinone oxidoreductase MWFE subunit (PF15879; HMM-score: 9.1)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic Membrane
    • Cytoplasmic Score: 0
    • Cytoplasmic Membrane Score: 10
    • Cellwall Score: 0
    • Extracellular Score: 0
    • Internal Helices: 8
  • LocateP: Multi-transmembrane
    • Prediction by SwissProt Classification: Membrane
    • Pathway Prediction: Sec-(SPI)
    • Intracellular possibility: 0.17
    • Signal peptide possibility: -1
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.02203
    • TAT(Tat/SPI): 0.004016
    • LIPO(Sec/SPII): 0.001284
  • predicted transmembrane helices (TMHMM): 8

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MSSASTQSTKTSDIHNESINKQMEAKAHETAQNADLKTEARSLFDNATKSIGRLAGNDESLNLNLKDMFSEVFKPHTKNEADEIFIAGTAKTTPAICDISEEWGKPWLFSRVFIAFTVTFIGLWVMAAIFNNTNAIPGLIFIGALTVPLSGLFFFYESNAFKNISIFEVIIMFFIGGVFSLLSTMVLYRFVVFSDQFERFGSLTFFDAFLVGLVEETGKALIIVYFVNKLKTNKILNGLLIGAAIGAGFAVFESAGYILNFALGENVPLLDIVFTRAWTAIGGHLVWSAIVGAAIVIAKEQHGFEFKDIFDKRFLIFFLSAVGLHGIWDTSLTVLGSDTLKIFILIVIVWILVFILMGAGLKQVNLLQKEFKEQQKKVDE

⊟Experimental data[edit | edit source]

  • experimentally validated: data available for COL, NCTC8325
  • protein localization: data available for COL
  • quantitative data / protein copy number per cell:
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator:

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

⊟Relevant publications[edit | edit source]

Christina N Krute, Harris Bell-Temin, Halie K Miller, Frances E Rivera, Andy Weiss, Stanley M Stevens, Lindsey N Shaw
The membrane protein PrsS mimics σS in protecting Staphylococcus aureus against cell wall-targeting antibiotics and DNA-damaging agents.
Microbiology (Reading): 2015, 161(Pt 5);1136-1148
[PubMed:25741016] [WorldCat.org] [DOI] (I p)

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