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NCBI: 06-JUL-2013

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus Newman
  • locus tag: NWMN_0248 [new locus tag: NWMN_RS01370 ]
  • pan locus tag?: SAUPAN001224000
  • symbol: NWMN_0248
  • pan gene symbol?: brnQ2
  • synonym:
  • product: branched-chain amino acid transport system II carrier protein

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: NWMN_0248 [new locus tag: NWMN_RS01370 ]
  • symbol: NWMN_0248
  • product: branched-chain amino acid transport system II carrier protein
  • replicon: chromosome
  • strand: -
  • coordinates: 301906..303213
  • length: 1308
  • essential: unknown other strains

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    TTGAAAAAACAAGTTATTATTTCGGGCCTCATGTTATTTTCACTATTTTTTGGAGCCGGA
    AATTTAATATTCCCGCCCATGCTTGGCCATACAGCGGGTCAAAATATGTGGATTGGTATG
    CTAGGCTTTGCCCTTACAGGCATATTACTCCCCTTTATTACTGTTATTGTTGTTGCATTT
    TATGATGAAGGTGTTGAAAGTGTAGGCAATCGTATACATCCATGGTTCGGGTTTATTTTT
    GCTGTCGTGATTTACATGTCTATCGGAGCATTTTACGGTATTCCACGTGCTGCAAATGTC
    GCGTACGAAATTGGTACAAGACACATTTTACCTGTGCATAACCAATGGACTTTAATTATA
    TTCGCAGCAATCTTTTTTGCCATCGTTTACTGGATTAGTTTAAATCCATCGAAAATCGTT
    GATAATTTAGGTAAATTATTAACACCGTTATTACTATTAATGGTCGCTCTATTAAGTATT
    GCTGTCATTTTCAACCCTGAATCTGCACTAAGTGCACCTAAGGATAAATATATAACACAT
    CCTTTCATTTCAGGAAGTTTGGAAGGCTATTTTACAATGGATCTTGTTGCTGCGTTAGCT
    TTTTCCGTAGTCATTGTCAATGGCTATAAGTTTAAAGGCCTCACAGATCGCATGAAAATT
    TTAAAATATGTCTGCTTTTCAGGTCTTATTGCAGCCATATTACTTGGAATGATTTACTTT
    GCACTTGCATACGTTGGGGCATCAACAGCTCCAGGAAACTTTAAAGATGGTACAGATATA
    TTGACGTACAACTCATTACGATTATTTGGTTCGTTCGGTAACCTCGTATTTGGAATGACG
    GTTATCCTTGCATGCCTAACAACATGTATAGGACTCGTCAATGCTTGCGCCACATTTACT
    AAGAAACACGTACCTAAGTTTTCTTATAAAATATTCGCACTTATTTTCTCTATCATAGGG
    TTCTTATTTACAACACTTGGTTTAGAAATGATTTTAAAAATTGCTGTCCCATTATTGACT
    TTAATATATCCCGTGTCGATTGCACTTGTACTCATATCATTTGCTAACATGTTTAGCACA
    TTCAGATTCAGTTGGGCCTATCGACTCGCAACTGTTATTACATTGATTATTTCAATTTTA
    CAAATACTAAATAGTTTCAACTTATTACACGGTGTTATTTTGAAATCGTTTATGATGTTA
    CCTTTAGCAGATATCGATTTAGCTTGGCTTGTACCATTCATGCTCTTTGCTATTATCGGT
    TTCATAATCGATGTATTTATACGCCGTCCGAAACAAGCGACAACTTAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1200
    1260
    1308

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: NWMN_0248 [new locus tag: NWMN_RS01370 ]
  • symbol: NWMN_0248
  • description: branched-chain amino acid transport system II carrier protein
  • length: 435
  • theoretical pI: 9.76448
  • theoretical MW: 48032.6
  • GRAVY: 1.04299

⊟Function[edit | edit source]

  • TIGRFAM:
    Metabolism Transport and binding proteins Amino acids, peptides and amines branched-chain amino acid transport system II carrier protein (TIGR00796; HMM-score: 404.8)
  • TheSEED: data available for COL, N315, NCTC8325, USA300_FPR3757
  • PFAM:
    APC (CL0062) Branch_AA_trans; Branched-chain amino acid transport protein (PF05525; HMM-score: 445.3)
    and 1 more
    no clan defined Plant_vir_prot; Plant viral movement protein (PF01307; HMM-score: 18.1)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic Membrane
    • Cytoplasmic Score: 0
    • Cytoplasmic Membrane Score: 10
    • Cellwall Score: 0
    • Extracellular Score: 0
    • Internal Helices: 12
  • LocateP: Multi-transmembrane
    • Prediction by SwissProt Classification: Membrane
    • Pathway Prediction: Sec-(SPI)
    • Intracellular possibility: 0.17
    • Signal peptide possibility: 0
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.083359
    • TAT(Tat/SPI): 0.00327
    • LIPO(Sec/SPII): 0.187431
  • predicted transmembrane helices (TMHMM): 12

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MKKQVIISGLMLFSLFFGAGNLIFPPMLGHTAGQNMWIGMLGFALTGILLPFITVIVVAFYDEGVESVGNRIHPWFGFIFAVVIYMSIGAFYGIPRAANVAYEIGTRHILPVHNQWTLIIFAAIFFAIVYWISLNPSKIVDNLGKLLTPLLLLMVALLSIAVIFNPESALSAPKDKYITHPFISGSLEGYFTMDLVAALAFSVVIVNGYKFKGLTDRMKILKYVCFSGLIAAILLGMIYFALAYVGASTAPGNFKDGTDILTYNSLRLFGSFGNLVFGMTVILACLTTCIGLVNACATFTKKHVPKFSYKIFALIFSIIGFLFTTLGLEMILKIAVPLLTLIYPVSIALVLISFANMFSTFRFSWAYRLATVITLIISILQILNSFNLLHGVILKSFMMLPLADIDLAWLVPFMLFAIIGFIIDVFIRRPKQATT

⊟Experimental data[edit | edit source]

  • experimentally validated: data available for COL, NCTC8325
  • protein localization: data available for COL
  • quantitative data / protein copy number per cell:
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator: CodY (repression) regulon
    CodY(TF)important in Amino acid metabolism; RegPrecise 

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

⊟Relevant publications[edit | edit source]

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