PangenomeCOLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 06-JUL-2013

⊟Summary

• organism: Staphylococcus aureus Newman
• locus tag: NWMN_0266
• pan locus tag^?: SAUPAN000544000
• symbol: NWMN_0266
• pan gene symbol^?: —
• synonym:
• product: hypothetical protein

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: NWMN_0266
• symbol: NWMN_0266
• product: hypothetical protein
• replicon: chromosome
• strand: -
• coordinates: 323762..324283
• length: 522
• essential: unknown

⊟Accession numbers

• Gene ID: 5330105 NCBI
• RefSeq: YP_001331300 NCBI
• BioCyc:
• MicrobesOnline: 3705797 MicrobesOnline

⊟Phenotype

Share your knowledge and add information here. [edit]

⊟DNA sequence

• 1
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  481
  
  ATGTTATGTTCTTTCAAAGCCATTTTAGATTTCAAAAGTTTAGTATCTATAGGCATTTCT
  TTTCACCACCTTTCGCATTACGTAAGTAATCTTATCATGATGTTACAAAAGAGGTCAAGC
  ATTTTACGAAAGTTTTTTAGAAAAATATTGCAAATGCCGAAAGTTTTCCTTATAATAGAA
  CTATCAAGTAAAAGGAGCTGTATTACGATGTGCTTTTCAAAAAGAATGAAACAATCAAGA
  GAAAAACAAGGTATGACTTTGGCCGAACTAGGAAGAAAAATTGGTAAAACTGAAGCTACT
  GTACAACGTTATGAAAGCGGAAATATCAAAAATCTAAAAAACGATACTATAGAAAGTATA
  GCTACTGCATTAAATGTTAATCCTGCGTATTTAATGGGGTGGGTTGAAGAAAACGATGAT
  GAAGTACAACATCGTGCAGCTCATTTAGAAGGAGAATTAACTGATGACGAGTGGCAAAGA
  GTTTTAGATTATGCAGATTATATAAGAAGTAAACGTAAGTAA
  60
  120
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  522
  

⊟Protein

⊟General

• locus tag: NWMN_0266
• symbol: NWMN_0266
• description: hypothetical protein
• length: 173
• theoretical pI: 9.95838
• theoretical MW: 20120.3
• GRAVY: -0.346243

⊟Function

• TIGRFAM:
  putative zinc finger/helix-turn-helix protein, YgiT family (TIGR03830; HMM-score: 25.4)
  and 7 more
  Genetic information processing Mobile and extrachromosomal element functions Prophage functions phage portal protein, HK97 family (TIGR01537; HMM-score: 16.5)
  Hypothetical proteins Conserved TIGR00270 family protein (TIGR00270; HMM-score: 16.4)
  RNA polymerase sigma factor, sigma-70 family (TIGR02937; HMM-score: 16)
  probable regulatory domain (TIGR03879; HMM-score: 14.2)
  Signal transduction Regulatory functions DNA interactions transcriptional regulator, y4mF family (TIGR03070; HMM-score: 14)
  Unknown function General mobile mystery protein A (TIGR02612; HMM-score: 12.7)
  Cellular processes Cellular processes Detoxification cyanase (TIGR00673; EC 4.2.1.104; HMM-score: 12.6)
• TheSEED:
• PFAM:
  HTH (CL0123) HTH_3; Helix-turn-helix (PF01381; HMM-score: 49.1)
  and 11 more
  HTH_31; Helix-turn-helix domain (PF13560; HMM-score: 39.2)
  HTH_26; Cro/C1-type HTH DNA-binding domain (PF13443; HMM-score: 38.6)
  HTH_19; Helix-turn-helix domain (PF12844; HMM-score: 31.8)
  Sigma70_r4; Sigma-70, region 4 (PF04545; HMM-score: 18.8)
  HTH_38; Helix-turn-helix domain (PF13936; HMM-score: 18.8)
  HTH_25; Helix-turn-helix domain (PF13413; HMM-score: 18.5)
  HTH_23; Homeodomain-like domain (PF13384; HMM-score: 17.3)
  HTH_IclR; IclR helix-turn-helix domain (PF09339; HMM-score: 13.8)
  Pou; Pou domain - N-terminal to homeobox domain (PF00157; HMM-score: 13.3)
  HTH_37; Helix-turn-helix domain (PF13744; HMM-score: 12.6)
  no clan defined GP57; Phage Tail fiber assembly helper protein (PF17594; HMM-score: 12.1)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors:
• effectors:

⊟Localization

• PSORTb: Cytoplasmic
  • Cytoplasmic Score: 7.5
  • Cytoplasmic Membrane Score: 1.15
  • Cellwall Score: 0.62
  • Extracellular Score: 0.73
  • Internal Helices: 0
• LocateP: Intracellular
  • Prediction by SwissProt Classification: Cytoplasmic
  • Pathway Prediction: No pathway
  • Intracellular possibility: 0.83
  • Signal peptide possibility: -1
  • N-terminally Anchored Score: 1
  • Predicted Cleavage Site: No CleavageSite
• SignalP: no predicted signal peptide
  • SP(Sec/SPI): 0.0024
  • TAT(Tat/SPI): 0.000213
  • LIPO(Sec/SPII): 0.000546
• predicted transmembrane helices (TMHMM): 0

⊟Accession numbers

• GI: 151220478 NCBI
• RefSeq: YP_001331300 NCBI
• UniProt: A0A0H3K604 UniProt

⊟Protein sequence

• MLCSFKAILDFKSLVSIGISFHHLSHYVSNLIMMLQKRSSILRKFFRKILQMPKVFLIIELSSKRSCITMCFSKRMKQSREKQGMTLAELGRKIGKTEATVQRYESGNIKNLKNDTIESIATALNVNPAYLMGWVEENDDEVQHRAAHLEGELTDDEWQRVLDYADYIRSKRK

⊟Experimental data

• experimentally validated:
• protein localization:
• quantitative data / protein copy number per cell:
• interaction partners:

⊟Expression & Regulation

⊟Operon

• MicrobesOnline: NWMN_0264 < NWMN_0265 < NWMN_0266

⊟Regulation

• regulator:

⊟Transcription pattern

• S.aureus Expression Data Browser: no data available

⊟Protein synthesis (provided by Aureolib)

• Aureolib: no data available

⊟Protein stability

• half-life: no data available

⊟Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊟Other Information

You are kindly invited to share additional interesting facts.

⊟Literature

⊟References

⊟Relevant publications