PangenomeCOLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 06-JUL-2013

⊟Summary

• organism: Staphylococcus aureus Newman
• locus tag: NWMN_0391 [new locus tag: NWMN_RS02210 ]
• pan locus tag^?: SAUPAN002107000
• symbol: set4nm
• pan gene symbol^?: ssl4
• synonym:
• product: superantigen-like protein

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: NWMN_0391 [new locus tag: NWMN_RS02210 ]
• symbol: set4nm
• product: superantigen-like protein
• replicon: chromosome
• strand: +
• coordinates: 439407..440333
• length: 927
• essential: unknown other strains

⊟Accession numbers

• Gene ID: 5332478 NCBI
• RefSeq: YP_001331425 NCBI
• BioCyc:
• MicrobesOnline: 3705922 MicrobesOnline

⊟Phenotype

Share your knowledge and add information here. [edit]

⊟DNA sequence

• 1
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  481
  541
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  661
  721
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  841
  901
  
  ATGAAAATAACAACGATTGCTAAAACAAGTTTAGCACTAGGCCTTTTAACAACAGGTGTA
  ATCACAACGACAACGCAAGCAGCAAACGCGACAACACCATCTTCCACTAAAGTGGAAGCA
  CCACAATCAACACCGCCCTCAACTAAAATAGAAGCACCGCAATCAAAACCAAACGCGACA
  ACACCGCCCTCAACTAAAGTAGAAGCACCGCAACAAACAGCAAATGCGACAACACCGCCT
  TCAACTAAAGTGACAACACCTCCATCAACAAACACGCCACAACCAATGCAATCTACTAAA
  TCAGACACACCACAATCGCCAACCACAAAACAAGTACCAACAGAAATAAATCCTAAATTT
  AAAGATTTAAGAGCGTATTATACGAAACCAAGTTTAGAATTTAAAAATGAGATTGGTATT
  ATTTTAAAAAAATGGACGACAATAAGATTTATGAATGTTGTCCCAGATTATTTCATATAT
  AAAATTGCTTTAGTTGGTAAAGATGATAAAAAATATGGTGAAGGAGTACATAGGAATGTC
  GATGTATTTGTCGTTTTAGAAGAAAATAATTACAATCTGGAAAAATATTCTGTCGGTGGT
  ATCACAAAGAGTAATAGTAAAAAAGTTGATCACAAAGCAGGAGTAAGAATTACTAAGGAA
  GATAATAAAGGTACAATCTCTCATGATGTTTCAGAATTCAAGATTACTAAAGAACAGATT
  TCCTTGAAAGAACTTGATTTTAAATTGAGAAAACAACTTATTGAAAAAAATAATCTGTAC
  GGTAACGTTGGTTCAGGTAAAATTGTTATTAAAATGAAAAACGGTGGAAAGTACACGTTT
  GAATTGCACAAAAAATTACAAGAAAATCGCATGGCAGATGTCATAGATGGCACTAATATT
  GATAACATTGAAGTGAATATAAAATAA
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  927
  

⊟Protein

• 4DXF
  PDB
• 4DXG
  PDB

Protein Data Bank: 4DXF

Protein Data Bank: 4DXG

⊟General

• locus tag: NWMN_0391 [new locus tag: NWMN_RS02210 ]
• symbol: Set4nm
• description: superantigen-like protein
• length: 308
• theoretical pI: 10.2076
• theoretical MW: 34055.7
• GRAVY: -0.638312

⊟Function

• TIGRFAM:
• TheSEED: data available for COL, N315, NCTC8325, USA300_FPR3757
• PFAM:
  OB_enterotoxin (CL0658) SSL_OB; Staphylococcal superantigen-like OB-fold domain (PF09199; HMM-score: 113.4)
  and 7 more
  Ant-toxin_C (CL0386) Stap_Strp_tox_C; Staphylococcal/Streptococcal toxin, beta-grasp domain (PF02876; HMM-score: 31.9)
  no clan defined Toxin_10; Insecticidal Crystal Toxin, P42 (PF05431; HMM-score: 12.3)
  Hamartin; Hamartin protein (PF04388; HMM-score: 12.1)
  TPR (CL0020) Suf; Suppressor of forked protein (Suf) (PF05843; HMM-score: 11.8)
  no clan defined THEG4; Testis highly expressed protein 4 (PF15834; HMM-score: 7.3)
  SOG2; RAM signalling pathway protein (PF10428; HMM-score: 6.7)
  Ndc1_Nup; Nucleoporin protein Ndc1-Nup (PF09531; HMM-score: 6.6)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors:
• effectors:

⊟Localization

• PSORTb: Extracellular
  • Cytoplasmic Score: 0
  • Cytoplasmic Membrane Score: 0
  • Cellwall Score: 0
  • Extracellular Score: 10
  • Internal Helices: 0
• LocateP: Secretory(released) (with CS)
  • Prediction by SwissProt Classification: Extracellular
  • Pathway Prediction: Sec-(SPI)
  • Intracellular possibility: -0.17
  • Signal peptide possibility: 1
  • N-terminally Anchored Score: -2
  • Predicted Cleavage Site: TQAANATT
• SignalP: Signal peptide SP(Sec/SPI) length 30 aa
  • SP(Sec/SPI): 0.973914
  • TAT(Tat/SPI): 0.012345
  • LIPO(Sec/SPII): 0.011819
  • Cleavage Site: CS pos: 30-31. ANA-TT. Pr: 0.5295
• predicted transmembrane helices (TMHMM): 0

⊟Accession numbers

• GI: 151220603 NCBI
• RefSeq: YP_001331425 NCBI
• UniProt: A0A0H3K6A3 UniProt

⊟Protein sequence

• MKITTIAKTSLALGLLTTGVITTTTQAANATTPSSTKVEAPQSTPPSTKIEAPQSKPNATTPPSTKVEAPQQTANATTPPSTKVTTPPSTNTPQPMQSTKSDTPQSPTTKQVPTEINPKFKDLRAYYTKPSLEFKNEIGIILKKWTTIRFMNVVPDYFIYKIALVGKDDKKYGEGVHRNVDVFVVLEENNYNLEKYSVGGITKSNSKKVDHKAGVRITKEDNKGTISHDVSEFKITKEQISLKELDFKLRKQLIEKNNLYGNVGSGKIVIKMKNGGKYTFELHKKLQENRMADVIDGTNIDNIEVNIK

⊟Experimental data

• experimentally validated: data available for NCTC8325
• protein localization: data available for COL
• quantitative data / protein copy number per cell:
• interaction partners:

⊟Expression & Regulation

⊟Operon

• MicrobesOnline: no polycistronic organisation predicted

⊟Regulation

• regulator:

⊟Transcription pattern

• S.aureus Expression Data Browser: data available for NCTC8325

⊟Protein synthesis (provided by Aureolib)

• Aureolib: no data available

⊟Protein stability

• half-life: no data available

⊟Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊟Other Information

You are kindly invited to share additional interesting facts.

⊟Literature

⊟References

⊟Relevant publications

Stefan Monecke, Peter Slickers, Ralf Ehricht
Assignment of Staphylococcus aureus isolates to clonal complexes based on microarray analysis and pattern recognition.
FEMS Immunol Med Microbiol: 2008, 53(2);237-51
[PubMed:18507678] [WorldCat.org] [DOI] (P p)