PangenomeCOLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 06-JUL-2013

⊟Summary

• organism: Staphylococcus aureus Newman
• locus tag: NWMN_0395 [new locus tag: NWMN_RS02235 ]
• pan locus tag^?: SAUPAN002114000
• symbol: set8nm
• pan gene symbol^?: ssl8
• synonym:
• product: superantigen-like protein

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: NWMN_0395 [new locus tag: NWMN_RS02235 ]
• symbol: set8nm
• product: superantigen-like protein
• replicon: chromosome
• strand: +
• coordinates: 444002..444700
• length: 699
• essential: unknown other strains

⊟Accession numbers

• Gene ID: 5332482 NCBI
• RefSeq: YP_001331429 NCBI
• BioCyc:
• MicrobesOnline: 3705926 MicrobesOnline

⊟Phenotype

Share your knowledge and add information here. [edit]

⊟DNA sequence

• 1
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  ATGAAATTTACAGTGATAGCTAAAGCGATATTTATATTAGGAATATTAACAACAAGTGTA
  ATGATAACAGAAAATCAATCGGTTAATGCAAAAGGAAAGTATGAAAAAATGAACCGTTTA
  TATGATACAAACAAGTTACATCAATACTATTCAGGACCTAGTTATGAGTTAACAAATGTT
  AGTGGCCAAAGTCAAGGTTATTATGACTCTAACGTTTTGCTTTTTAACCAACAAAATCAA
  AAGTTCCAAGTGTTTTTATTGGGAAAAGATGAAAATAAATACAAAGAAAAAACACATGGT
  TTAGATGTCTTTGCGGTACCAGAATTAGTAGATTTAGATGGAAGAATATTTAGTGTTAGT
  GGTGTAACAAAGAAAAACGTAAAATCAATATTTGAGTCTCTAAGAACGCCGAACTTACTA
  GTTAAAAAAATAGACGATAAAGACGGTTTTTCTATTGATGAATTTTTCTTTATTCAAAAG
  GAAGAAGTGTCATTGAAGGAACTTGATTTTAAAATAAGAAAACTGTTGATTAAAAAATAC
  AAACTGTATGAAGGGTCAGCTGATAAAGGTAGAATTGTTATTAATATGAAAGATGAAAAT
  AAGTATGAAATTGATTTAAGTGATAAATTAGATTTCGAGCGTATGGCAGATGTCATTAAT
  AGTGAACAAATTAAAAACATCGAAGTGAATTTGAAATAA
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  699
  

⊟Protein

• 3R2T
  PDB

Protein Data Bank: 3R2T

⊟General

• locus tag: NWMN_0395 [new locus tag: NWMN_RS02235 ]
• symbol: Set8nm
• description: superantigen-like protein
• length: 232
• theoretical pI: 9.0036
• theoretical MW: 26874.7
• GRAVY: -0.448707

⊟Function

• TIGRFAM:
• TheSEED: data available for N315, NCTC8325, USA300_FPR3757
• PFAM:
  OB_enterotoxin (CL0658) SSL_OB; Staphylococcal superantigen-like OB-fold domain (PF09199; HMM-score: 109.3)
  and 2 more
  Ant-toxin_C (CL0386) Stap_Strp_tox_C; Staphylococcal/Streptococcal toxin, beta-grasp domain (PF02876; HMM-score: 39.9)
  no clan defined AMIN; AMIN domain (PF11741; HMM-score: 13.2)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors:
• effectors:

⊟Localization

• PSORTb: Extracellular
  • Cytoplasmic Score: 0
  • Cytoplasmic Membrane Score: 0
  • Cellwall Score: 0
  • Extracellular Score: 10
  • Internal Helix: 1
• LocateP: N-terminally anchored (No CS)
  • Prediction by SwissProt Classification: Membrane
  • Pathway Prediction: Sec-(SPI)
  • Intracellular possibility: -0.17
  • Signal peptide possibility: 1
  • N-terminally Anchored Score: 2
  • Predicted Cleavage Site: No CleavageSite
• SignalP: Signal peptide SP(Sec/SPI) length 30 aa
  • SP(Sec/SPI): 0.983392
  • TAT(Tat/SPI): 0.002802
  • LIPO(Sec/SPII): 0.004913
  • Cleavage Site: CS pos: 30-31. VNA-KG. Pr: 0.9010
• predicted transmembrane helices (TMHMM): 0

⊟Accession numbers

• GI: 162415895 NCBI
• RefSeq: YP_001331429 NCBI
• UniProt: A0A0H3KEE4 UniProt

⊟Protein sequence

• MKFTVIAKAIFILGILTTSVMITENQSVNAKGKYEKMNRLYDTNKLHQYYSGPSYELTNVSGQSQGYYDSNVLLFNQQNQKFQVFLLGKDENKYKEKTHGLDVFAVPELVDLDGRIFSVSGVTKKNVKSIFESLRTPNLLVKKIDDKDGFSIDEFFFIQKEEVSLKELDFKIRKLLIKKYKLYEGSADKGRIVINMKDENKYEIDLSDKLDFERMADVINSEQIKNIEVNLK

⊟Experimental data

• experimentally validated: data available for NCTC8325
• protein localization:
• quantitative data / protein copy number per cell:
• interaction partners:

⊟Expression & Regulation

⊟Operon

• MicrobesOnline: no polycistronic organisation predicted

⊟Regulation

• regulator:

⊟Transcription pattern

• S.aureus Expression Data Browser: data available for NCTC8325

⊟Protein synthesis (provided by Aureolib)

• Aureolib: no data available

⊟Protein stability

• half-life: no data available

⊟Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊟Other Information

You are kindly invited to share additional interesting facts.

⊟Literature

⊟References

⊟Relevant publications

Stefan Monecke, Peter Slickers, Ralf Ehricht
Assignment of Staphylococcus aureus isolates to clonal complexes based on microarray analysis and pattern recognition.
FEMS Immunol Med Microbiol: 2008, 53(2);237-51
[PubMed:18507678] [WorldCat.org] [DOI] (P p)