From AureoWiki
Jump to navigation Jump to search

NCBI: 06-JUL-2013

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus Newman
  • locus tag: NWMN_0412 [new locus tag: NWMN_RS02325 ]
  • pan locus tag?: SAUPAN002135000
  • symbol: lpl9nm
  • pan gene symbol?: lpl9
  • synonym:
  • product: tandem lipoprotein

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: NWMN_0412 [new locus tag: NWMN_RS02325 ]
  • symbol: lpl9nm
  • product: tandem lipoprotein
  • replicon: chromosome
  • strand: +
  • coordinates: 460936..461748
  • length: 813
  • essential: unknown other strains

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    ATGGAGTATATAAAAAAAATTGCTTTGTACATGAGTGTATTACTTTTAATCATTTTTATT
    GGGGGATGTGGAAATATGAAAGATGAACAGAAAAAAGAGGAACAAACGAATAAAACAGAT
    TCAAAAGAAGAACAAATCAAAAAGAGTTTTGCGAAAACGTTAGATATGTATCCAATTAAG
    AATCTCGAGGATTTATACGACAAAGAAGGATATCGAGATGGTGAGTTTAAAAAAGGCGAT
    AAAGGTACGTGGACTATACTTACAGGTTTTTCAAAAAGTAACAAACCAGGAGTATTAGAT
    GATGAAGGCATGGTGTTATATCTTAATAGAAATACCAAAAAGGCAACAGGTTATTATTTT
    GTAAATAAAGTTTATGATGATATTAGCAAAAATCATAATGAGAAAAAATATCGTGTTGAA
    CTAAAAAATAATAAGATTGTTCTTTTGGATAATGTAGAAGACAAAAAACTTAAACAAAAA
    ATTGAAAATTTTAAATTTTTTAGTCAGTATGCTGATTTTAAAGATTTAAAAAATTATCAA
    GATGGAAATATAACAACTAATGAAAACGTACCGAGCTATGAAGCACAATATAAAATGAAC
    AATAGTGATAAAAATGTAAAAAAACTTAGAGAAATTTATCCAATTACAACTAATAACTCT
    CCAAATCTAAAATTATATATAGATGGTGATATAAAAGGAAGCTCAGTAGGATATAAAAAA
    ATAGAATATAAATTTTCAAAAGATAAAGGTCAAGAGACAACATTAAGAGATTATTTGAAT
    TTTGGACCGTCTGAAGGTGAGAATGTTGAGTAG
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    813

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: NWMN_0412 [new locus tag: NWMN_RS02325 ]
  • symbol: Lpl9nm
  • description: tandem lipoprotein
  • length: 270
  • theoretical pI: 9.22493
  • theoretical MW: 31467.4
  • GRAVY: -0.971852

⊟Function[edit | edit source]

  • TIGRFAM:
    Staphylococcus tandem lipoproteins (TIGR01742; HMM-score: 354.6)
  • TheSEED: data available for COL, N315, NCTC8325, USA300_FPR3757
  • PFAM:
    LolA_LolB (CL0048) DUF576; Csa1 family (PF04507; HMM-score: 383.1)
    and 2 more
    no clan defined FidL_like; FidL-like putative membrane protein (PF15941; HMM-score: 11.1)
    ABC-2 (CL0181) ABC2_membrane_3; ABC-2 family transporter protein (PF12698; HMM-score: 7)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: unknown (no significant prediction)
    • Cytoplasmic Score: 0
    • Cytoplasmic Membrane Score: 3.33
    • Cellwall Score: 3.33
    • Extracellular Score: 3.33
    • Internal Helix: 1
  • LocateP: Lipid anchored
    • Prediction by SwissProt Classification: Extracellular
    • Pathway Prediction: Sec-(SPII)
    • Intracellular possibility: 0.17
    • Signal peptide possibility: -1
    • N-terminally Anchored Score: -1
    • Predicted Cleavage Site: IFIGGCG
  • SignalP: Signal peptide LIPO(Sec/SPII) length 22 aa
    • SP(Sec/SPI): 0.000413
    • TAT(Tat/SPI): 0.000056
    • LIPO(Sec/SPII): 0.999261
    • Cleavage Site: CS pos: 22-23. IGG-CG. Pr: 0.9998
  • predicted transmembrane helices (TMHMM): 1

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MEYIKKIALYMSVLLLIIFIGGCGNMKDEQKKEEQTNKTDSKEEQIKKSFAKTLDMYPIKNLEDLYDKEGYRDGEFKKGDKGTWTILTGFSKSNKPGVLDDEGMVLYLNRNTKKATGYYFVNKVYDDISKNHNEKKYRVELKNNKIVLLDNVEDKKLKQKIENFKFFSQYADFKDLKNYQDGNITTNENVPSYEAQYKMNNSDKNVKKLREIYPITTNNSPNLKLYIDGDIKGSSVGYKKIEYKFSKDKGQETTLRDYLNFGPSEGENVE

⊟Experimental data[edit | edit source]

  • experimentally validated: data available for COL, NCTC8325
  • protein localization: data available for COL
  • quantitative data / protein copy number per cell:
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator: SaeR (activation) regulon
    SaeR(TF)important in Virulence; RegPrecise    transcription unit transferred from N315 data RegPrecise 

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

⊟Relevant publications[edit | edit source]

Retrieved from "http://aureowiki.med.uni-greifswald.de/index.php?title=NWMN_0412&oldid=62022"