PangenomeCOLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 10-JUN-2013

⊟Summary

• organism: Staphylococcus aureus USA300_FPR3757
• locus tag: SAUSA300_0419 [new locus tag: SAUSA300_RS02245 ]
• pan locus tag^?: SAUPAN002135000
• symbol: SAUSA300_0419
• pan gene symbol^?: lpl9
• synonym:
• product: tandem lipoprotein

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: SAUSA300_0419 [new locus tag: SAUSA300_RS02245 ]
• symbol: SAUSA300_0419
• product: tandem lipoprotein
• replicon: chromosome
• strand: +
• coordinates: 471340..472152
• length: 813
• essential: unknown other strains

⊟Accession numbers

• Gene ID: 3912926 NCBI
• RefSeq: YP_493132 NCBI
• BioCyc: see SAUSA300_RS02245
• MicrobesOnline: 1291934 MicrobesOnline

⊟Phenotype

Share your knowledge and add information here. [edit]

⊟DNA sequence

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  ATGGAGTATATAAAAAAAATTGCTTTGTACATGAGTGTATTACTTTTAATCATTTTTATT
  GGGGGATGTGGAAATATGAAAGATGAACAGAAAAAAGAGGAACAAACGAATAAAACAGAT
  TCAAAAGAAGAACAAATCAAAAAGAGTTTTGCGAAAACGTTAGATATGTATCCAATTAAG
  AATCTCGAGGATTTATACGACAAAGAAGGATATCGAGATGGTGAGTTTAAAAAAGGCGAT
  AAAGGTACGTGGACTATACTTACAGGTTTTTCAAAAAGTAACAAACCAGGAGTATTAGAT
  GATGAAGGCATGGTGTTATATCTTAATAGAAATGCCAAAAAGGCAACAGGTTATTATTTT
  GTAAATAAAGTTTATGATGATATTAGCAAAAATCATAATGAGAAAAAATATCGTGTTGAA
  CTAAAAAATAATAAGATTGTTCTTTTGGATAATGTAGAAGACAAAAAACTTAAACAAAAA
  ATTGAAAATTTTAAATTTTTTAGTCAGTATGCTGATTTTAAAGATTTAAAAAATTATCAA
  GATGGAAATATAACAACTAATGAAAACGTACCGAGCTATGAAGCACAATATAAAATGAAC
  AATAGTGATAAAAATGTAAAAAAACTTAGAGAAATTTATCCAATTACAACTAATAACTCT
  CCAAATCTAAAATTATATATAGATGGTGATATAAAAGGAAGCTCAGTAGGATATAAAAAA
  ATAGAATATAAATTTTCAAAAGATAAAGGTCAAGAGACAACATTAAGAGATTATTTGAAT
  TTTGGACCGTCTGAAGGTGAGAATGTTGAGTAG
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⊟Protein

⊟General

• locus tag: SAUSA300_0419 [new locus tag: SAUSA300_RS02245 ]
• symbol: SAUSA300_0419
• description: tandem lipoprotein
• length: 270
• theoretical pI: 9.22493
• theoretical MW: 31437.4
• GRAVY: -0.962593

⊟Function

• TIGRFAM:
  Staphylococcus tandem lipoproteins (TIGR01742; HMM-score: 352.7)
• TheSEED  :
  • Tandem lipoprotein within Pathogenicity island
• PFAM:
  LolA_LolB (CL0048) DUF576; Csa1 family (PF04507; HMM-score: 380.5)
  and 2 more
  no clan defined FidL_like; FidL-like putative membrane protein (PF15941; HMM-score: 11.3)
  ABC-2 (CL0181) ABC2_membrane_3; ABC-2 family transporter protein (PF12698; HMM-score: 6.8)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors:
• effectors:

⊟Localization

• PSORTb: unknown (no significant prediction)
  • Cytoplasmic Score: 0
  • Cytoplasmic Membrane Score: 3.33
  • Cellwall Score: 3.33
  • Extracellular Score: 3.33
  • Internal Helix: 1
• LocateP: Lipid anchored
  • Prediction by SwissProt Classification: Extracellular
  • Pathway Prediction: Sec-(SPII)
  • Intracellular possibility: 0.17
  • Signal peptide possibility: -1
  • N-terminally Anchored Score: -1
  • Predicted Cleavage Site: IFIGGCG
• SignalP: Signal peptide LIPO(Sec/SPII) length 22 aa
  • SP(Sec/SPI): 0.000413
  • TAT(Tat/SPI): 0.000056
  • LIPO(Sec/SPII): 0.999261
  • Cleavage Site: CS pos: 22-23. IGG-CG. Pr: 0.9998
• predicted transmembrane helices (TMHMM): 1

⊟Accession numbers

• GI: 87160015 NCBI
• RefSeq: YP_493132 NCBI
• UniProt: Q2FJJ6 UniProt

⊟Protein sequence

• MEYIKKIALYMSVLLLIIFIGGCGNMKDEQKKEEQTNKTDSKEEQIKKSFAKTLDMYPIKNLEDLYDKEGYRDGEFKKGDKGTWTILTGFSKSNKPGVLDDEGMVLYLNRNAKKATGYYFVNKVYDDISKNHNEKKYRVELKNNKIVLLDNVEDKKLKQKIENFKFFSQYADFKDLKNYQDGNITTNENVPSYEAQYKMNNSDKNVKKLREIYPITTNNSPNLKLYIDGDIKGSSVGYKKIEYKFSKDKGQETTLRDYLNFGPSEGENVE

⊟Experimental data

• experimentally validated: data available for COL, NCTC8325
• protein localization: data available for COL
• quantitative data / protein copy number per cell:
• interaction partners:

⊟Expression & Regulation

⊟Operon

• MicrobesOnline: SAUSA300_0419 > SAUSA300_0420 > SAUSA300_0421 > SAUSA300_0422

⊟Regulation

• regulator: SaeR (activation) regulon

  SaeR

  (TF)

  important in Virulence; RegPrecise    transcription unit transferred from N315 data RegPrecise

⊟Transcription pattern

• S.aureus Expression Data Browser: data available for NCTC8325

⊟Protein synthesis (provided by Aureolib)

• Aureolib: no data available

⊟Protein stability

• half-life: no data available

⊟Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊟Other Information

You are kindly invited to share additional interesting facts.

⊟Literature

⊟References

⊟Relevant publications